Restriction map for
pFN21AE0037
(Circular) (Six-base) MAPSORT of: pFN21AE0037.seq Check: 9462 from: 1 to: 6925
pFN21AE0037 6925 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4735 278
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 4268 1937
Size: 1078 1253 4594
Fragments arranged by size:
4594 1253 1078
Acc65I G'GTAC_C
Cuts at: 2715 4246 2715
Size: 1531 5394
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 3597 3673 4155 4988 6372 6640 1632
Size: 1583 382 76 482 833 1384 268 1917
Fragments arranged by size:
1917 1583 1384 833 482 382 268 76
AfeI AGC'GCT
Cuts at: 2261 2261
Size: 6925
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 5824 6801 1203
Size: 4621 977 1327
Fragments arranged by size:
4621 1327 977
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 2275 5654 2275
Size: 3379 3546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2178 2956 3818 1184
Size: 994 778 862 4291
Fragments arranged by size:
4291 994 862 778
AlwNI CAG_nnn'CTG
Cuts at: 2122 2251 2660 3437 3899 4127 4668 6240 2122
Size: 129 409 777 462 228 541 1572 2807
Fragments arranged by size:
2807 1572 777 541 462 409 228 129
ApaI G_GGCC'C
Cuts at: 2369 3832 2369
Size: 1463 5462
ApaLI G'TGCA_C
Cuts at: 3122 4982 6138 3122
Size: 1860 1156 3909
Fragments arranged by size:
3909 1860 1156
ApoI r'AATT_y
Cuts at: 1493 3278 4234 4460 4612 6721 1493
Size: 1785 956 226 152 2109 1697
Fragments arranged by size:
2109 1785 1697 956 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5479 160
Size: 872 4447 1606
Fragments arranged by size:
4447 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 3682 4084 4250 1870
Size: 73 283 1456 402 166 4545
Fragments arranged by size:
4545 1456 402 283 166 73
BamHI G'GATC_C
Cuts at: 2172 3465 4255 4622 2172
Size: 1293 790 367 4475
Fragments arranged by size:
4475 1293 790 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 2715 3149 3203 4054
Size: 325 217 627 774 154 434 54 851
Cuts at: 4054 4246 5701 618
Size: 192 1455 1842
Fragments arranged by size:
1842 1455 851 774 627 434 325 217
192 154 54
BanII G_rGCy'C
Cuts at: 1391 2369 2687 3171 3264 3832 4070 4244 1391
Size: 978 318 484 93 568 238 174 4072
Fragments arranged by size:
4072 978 568 484 318 238 174 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2526 3394 928
Size: 971 627 868 4459
Fragments arranged by size:
4459 971 868 627
BbvCI CC'TCA_GC
Cuts at: 3032 3249 4146 3032
Size: 217 897 5811
Fragments arranged by size:
5811 897 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3208 3242 5116 5150 1903
Size: 34 50 34 1187 34 1874 34 3678
Fragments arranged by size:
3678 1874 1187 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4817 6027 4817
Size: 1210 5715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6925
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5536 136
Size: 107 122 71 631 605 3864 1525
Fragments arranged by size:
3864 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3820 6920 3820
Size: 3100 3825
BlpI GC'TnA_GC
Cuts at: 2681 4163 4342 2681
Size: 1482 179 5264
Fragments arranged by size:
5264 1482 179
Bme1580I G_kGCm'C
Cuts at: 1790 2369 3126 3206 3832 4057 4986 6142 1790
Size: 579 757 80 626 225 929 1156 2573
Fragments arranged by size:
2573 1156 929 757 626 579 225 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2382 2945 4656
Size: 416 53 83 399 24 965 563 1711
Cuts at: 4656 5614 442
Size: 958 1753
Fragments arranged by size:
1753 1711 965 958 563 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2903 3442 2761
Size: 142 539 6244
Fragments arranged by size:
6244 539 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2159 2182 2203 3047 3185 3857 4090
Size: 911 9 23 21 844 138 672 233
Cuts at: 4090 4205 5585 1239
Size: 115 1380 2579
Fragments arranged by size:
2579 1380 911 844 672 233 138 115
23 21 9
Bpu10I CC'TnA_GC
Cuts at: 3032 3249 3446 4146 3032
Size: 217 197 700 5811
Fragments arranged by size:
5811 700 217 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5050 5915 6213 5050
Size: 865 298 5762
Fragments arranged by size:
5762 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 2874 5588 882
Size: 617 636 739 2714 2219
Fragments arranged by size:
2714 2219 739 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 2307 3091 4716 493
Size: 711 1103 784 1625 2702
Fragments arranged by size:
2702 1625 1103 784 711
BsaBI GATnn'nnATC
Cuts at: 6919 6919
Size: 6925
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3211 3380 4732 5114
Size: 53 83 186 936 1678 169 1352 382
Cuts at: 5114 5749 275
Size: 635 1451
Fragments arranged by size:
1678 1451 1352 936 635 382 186 169
83 53
BsaWI w'CCGG_w
Cuts at: 2397 2831 3468 3600 3960 5358 5756 6030 6177
Size: 434 637 132 360 1398 398 274 147
Cuts at: 6177 6588 2397
Size: 411 2734
Fragments arranged by size:
2734 1398 637 434 411 398 360 274
147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 2472 2502 1178
Size: 30 165 30 798 30 241 30 5601
Fragments arranged by size:
5601 798 241 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2480 3266 3562 1186
Size: 1234 60 786 296 4549
Fragments arranged by size:
4549 1234 786 296 60
BseYI C'CCAG_C
Cuts at: 2029 2035 2059 2636 2914 2986 3117 3140 3240
Size: 6 24 577 278 72 131 23 100
Cuts at: 3240 3509 3813 6128 6624 2029
Size: 269 304 2315 496 2330
Fragments arranged by size:
2330 2315 577 496 304 278 269 131
100 72 24 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 3392 1163
Size: 573 528 1128 4696
Fragments arranged by size:
4696 1128 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2295 4375 5136 6164 1540
Size: 275 186 294 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2687 2935 3126 3171 4050 4986 5071 6142 2687
Size: 248 191 45 879 936 85 1071 3470
Fragments arranged by size:
3470 1071 936 879 248 191 85 45
BsiWI C'GTAC_G
Cuts at: 2307 2307
Size: 6925
BsmI GAATG_Cn'
Cuts at: 4436 4529 4436
Size: 93 6832
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2687 2935 3126 3171 3206 3264
Size: 399 579 318 248 191 45 35 58
Cuts at: 3264 3832 4050 4057 4070 4244 4986 5071 6142
Size: 568 218 7 13 174 742 85 1071
Cuts at: 6142 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 579 568 399 318 248
218 191 174 85 58 45 35 13
7
BspEI T'CCGG_A
Cuts at: 2831 3960 6588 2831
Size: 1129 2628 3168
Fragments arranged by size:
3168 2628 1129
BspHI T'CATG_A
Cuts at: 4814 6544 4814
Size: 1730 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2892 3946 4280 844
Size: 1883 165 1054 334 3489
Fragments arranged by size:
3489 1883 1054 334 165
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 4812 1394
Size: 429 1603 1386 3507
Fragments arranged by size:
3507 1603 1386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 3582 5420 5594 65
Size: 1183 1317 1017 1838 174 1396
Fragments arranged by size:
1838 1396 1317 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5569 5756 1700
Size: 110 3759 187 2869
Fragments arranged by size:
3759 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 4372
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 4985 5997 4985
Size: 1012 5913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 3899 794
Size: 1457 1648 3820
Fragments arranged by size:
3820 1648 1457
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6925
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2353 2922 3052 2107
Size: 246 569 130 5980
Fragments arranged by size:
5980 569 246 130
BstYI r'GATC_y
Cuts at: 1972 2133 2172 3420 3465 3621 3820 4130 4255
Size: 161 39 1248 45 156 199 310 125
Cuts at: 4255 4622 5007 5024 6476 6562 6574 6920 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 310 199
161 156 125 86 45 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2728 3365 4205 2728
Size: 637 840 5448
Fragments arranged by size:
5448 840 637
BtgI C'CryG_G
Cuts at: 513 1065 3064 3273 3576 513
Size: 552 1999 209 303 3862
Fragments arranged by size:
3862 1999 552 303 209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6186
Fragments arranged by size:
6186 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 3151 3882 3926 4441 5235 5255 2015
Size: 1136 731 44 515 794 20 3685
Fragments arranged by size:
3685 1136 794 731 515 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6925
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6890
DraI TTT'AAA
Cuts at: 4229 4229
Size: 6925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2292 4372
Size: 54 1425 50 275 18 244 218 2080
Cuts at: 4372 5261 6890 8
Size: 889 1629 43
Fragments arranged by size:
2080 1629 1425 889 275 244 218 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2292 4372 1537
Size: 275 480 2080 4090
Fragments arranged by size:
4090 2080 480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2806 3046 3282 4855 1353
Size: 1453 240 236 1573 3423
Fragments arranged by size:
3423 1573 1453 240 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5512 5886 6032 387
Size: 1064 138 3923 374 146 1280
Fragments arranged by size:
3923 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2685 3169 2685
Size: 484 6441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2159 2182 2203 3047 3185 3215
Size: 393 518 9 23 21 844 138 30
Cuts at: 3215 3597 3673 3857 4090 4155 4205 4988 5585
Size: 382 76 184 233 65 50 783 597
Cuts at: 5585 6372 6640 1239
Size: 787 268 1524
Fragments arranged by size:
1524 844 787 783 597 518 393 382
268 233 184 138 76 65 50 30
23 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4105 4105
Size: 6925
EcoO109I rG'GnC_Cy
Cuts at: 2119 2236 2269 2366 2485 2767 2957 3186 3434
Size: 117 33 97 119 282 190 229 248
Cuts at: 3434 3828 3829 3860 2119
Size: 394 1 31 5184
Fragments arranged by size:
5184 394 282 248 229 190 119 117
97 33 31 1
EcoRI G'AATT_C
Cuts at: 3278 4234 4612 3278
Size: 956 378 5591
Fragments arranged by size:
5591 956 378
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 2263 6072 1678
Size: 585 3809 2531
Fragments arranged by size:
3809 2531 585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 5566 5598 5640
Size: 32 135 32 689 32 3273 32 42
Cuts at: 5640 5672 1373
Size: 32 2626
Fragments arranged by size:
3273 2626 689 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4269 4515 669
Size: 988 281 2331 246 3079
Fragments arranged by size:
3079 2331 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4137 748
Size: 3389 3536
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KpnI G_GTAC'C
Cuts at: 2719 4250 2719
Size: 1531 5394
MfeI C'AATT_G
Cuts at: 4524 4524
Size: 6925
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3069 6039 6223 1305
Size: 1764 2970 184 2007
Fragments arranged by size:
2970 2007 1764 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5103
Fragments arranged by size:
5103 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3344 4172 4883 5242 5401 518
Size: 648 2166 12 828 711 359 159 2042
Fragments arranged by size:
2166 2042 828 711 648 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2158 2248 3117 3719 4341 5018 6166 6411
Size: 552 90 869 602 622 677 1148 245
Cuts at: 6411 1606
Size: 2120
Fragments arranged by size:
2120 1148 869 677 622 602 552 245
90
NcoI C'CATG_G
Cuts at: 513 1065 3576 513
Size: 552 2511 3862
Fragments arranged by size:
3862 2511 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6925
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NspI r_CATG'y
Cuts at: 1132 1229 4283 5828 1132
Size: 97 3054 1545 2229
Fragments arranged by size:
3054 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6925
PciI A'CATG_T
Cuts at: 5824 5824
Size: 6925
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2122 2712 3437 1108
Size: 902 112 590 725 4596
Fragments arranged by size:
4596 902 725 590 112
PfoI T'CCnGG_A
Cuts at: 2136 2175 2868 3162 2136
Size: 39 693 294 5899
Fragments arranged by size:
5899 693 294 39
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2236 2269 2767 2957 3186 2119
Size: 117 33 498 190 229 5858
Fragments arranged by size:
5858 498 229 190 117 33
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 4495 4495
Size: 6925
PspOMI G'GGCC_C
Cuts at: 2365 3828 2365
Size: 1463 5462
PstI C_TGCA'G
Cuts at: 830 2259 3309 3807 3877 3904 3933 3957 4277
Size: 1429 1050 498 70 27 29 24 320
Cuts at: 4277 830
Size: 3478
Fragments arranged by size:
3478 1429 1050 498 320 70 29 27
24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 2158 2248 3117 2158
Size: 90 869 5966
Fragments arranged by size:
5966 869 90
SacI G_AGCT'C
Cuts at: 2687 3171 2687
Size: 484 6441
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SanDI GG'GwC_CC
Cuts at: 2767 2957 3186 2767
Size: 190 229 6506
Fragments arranged by size:
6506 229 190
SbfI CC_TGCA'GG
Cuts at: 3807 3957 4277 3807
Size: 150 320 6455
Fragments arranged by size:
6455 320 150
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SexAI A'CCwGG_T
Cuts at: 2238 2238
Size: 6925
SfcI C'TryA_G
Cuts at: 826 1045 2255 2534 2947 3305 3778 3803 3873
Size: 219 1210 279 413 358 473 25 70
Cuts at: 3873 3900 3929 3953 4036 4273 5408 6089 6280
Size: 27 29 24 83 237 1135 681 191
Cuts at: 6280 826
Size: 1471
Fragments arranged by size:
1471 1210 1135 681 473 413 358 279
237 219 191 83 70 29 27 25
24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 3684 4086 4252 1872
Size: 1812 402 166 4545
Fragments arranged by size:
4545 1812 402 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5029 5930 6192 820
Size: 197 926 3086 901 262 1553
Fragments arranged by size:
3086 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 2283 2300 4210 1517
Size: 766 17 1910 4232
Fragments arranged by size:
4232 1910 766 17
StyI C'CwwG_G
Cuts at: 513 1065 2604 3576 3906 4364 513
Size: 552 1539 972 330 458 3074
Fragments arranged by size:
3074 1539 972 552 458 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3109 3515 4952 4969 5122 5307 5752
Size: 1255 1529 406 1437 17 153 185 445
Cuts at: 5752 325
Size: 1498
Fragments arranged by size:
1529 1498 1437 1255 445 406 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 5171 6883
Size: 274 80 33 51 1443 672 2522 1712
Cuts at: 6883 96
Size: 138
Fragments arranged by size:
2522 1712 1443 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 2472 2504 3196 3228 6885 6917 2201
Size: 32 239 32 692 32 3657 32 2209
Fragments arranged by size:
3657 2209 692 239 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3821 3821
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6925
XmaI C'CCGG_G
Cuts at: 1870 3682 4084 4250 1870
Size: 1812 402 166 4545
Fragments arranged by size:
4545 1812 402 166
XmnI GAAnn'nnTTC
Cuts at: 2700 5054 2700
Size: 2354 4571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4733 276
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SapI SfiI SfoI SrfI SwaI