Restriction map for
pFN21AE0036
(Circular) (Six-base) MAPSORT of: pFN21AE0036.seq Check: 5551 from: 1 to: 6850
pFN21AE0036 6850 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3113 3113
Size: 6850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4660 278
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4193 1937
Size: 2256 4594
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 4977 5350 1563
Size: 3414 373 3063
Fragments arranged by size:
3414 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4054 4913 6297 6565 1632
Size: 2422 859 1384 268 1917
Fragments arranged by size:
2422 1917 1384 859 268
AfeI AGC'GCT
Cuts at: 2423 3023 2423
Size: 600 6250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6653
AflIII A'CryG_T
Cuts at: 1203 5749 6726 1203
Size: 4546 977 1327
Fragments arranged by size:
4546 1327 977
AgeI A'CCGG_T
Cuts at: 2239 4040 5681 2239
Size: 1801 1641 3408
Fragments arranged by size:
3408 1801 1641
AhdI GACnn_n'nnGTC
Cuts at: 2221 5579 2221
Size: 3358 3492
AleI CACnn'nnGTG
Cuts at: 1166 2351 1166
Size: 1185 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2890 1184
Size: 1706 5144
AlwNI CAG_nnn'CTG
Cuts at: 2397 2503 3954 4593 6165 2397
Size: 106 1451 639 1572 3082
Fragments arranged by size:
3082 1572 1451 639 106
ApaI G_GGCC'C
Cuts at: 3046 3046
Size: 6850
ApaLI G'TGCA_C
Cuts at: 4907 6063 4907
Size: 1156 5694
ApoI r'AATT_y
Cuts at: 1493 3757 3814 4008 4159 4385 4537 6646 1493
Size: 2264 57 194 151 226 152 2109 1697
Fragments arranged by size:
2264 2109 1697 226 194 152 151 57
AseI AT'TA_AT
Cuts at: 160 1032 5404 160
Size: 872 4372 1606
Fragments arranged by size:
4372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 3330 3885 3936 4089 4175 1870
Size: 73 1387 555 51 153 86 4545
Fragments arranged by size:
4545 1387 555 153 86 73 51
BamHI G'GATC_C
Cuts at: 4180 4547 4180
Size: 367 6483
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 4171 5626 618
Size: 325 217 627 1079 1305 1455 1842
Fragments arranged by size:
1842 1455 1305 1079 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2668 3046 3624 3744 3886 3937 4169 1391
Size: 1277 378 578 120 142 51 232 4072
Fragments arranged by size:
4072 1277 578 378 232 142 120 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3391 928
Size: 971 1492 4387
Fragments arranged by size:
4387 1492 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2171 2205 3468 3502 5041
Size: 34 50 34 150 34 1263 34 1539
Cuts at: 5041 5075 1903
Size: 34 3678
Fragments arranged by size:
3678 1539 1263 150 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 4742 5952 4124
Size: 618 1210 5022
Fragments arranged by size:
5022 1210 618
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6850
BfrBI ATG'CAT
Cuts at: 2008 2284 2008
Size: 276 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2738 5461 136
Size: 107 122 71 631 605 1066 2723 1525
Fragments arranged by size:
2723 1525 1066 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6845 6845
Size: 6850
BlpI GC'TnA_GC
Cuts at: 3770 3864 4267 3770
Size: 94 403 6353
Fragments arranged by size:
6353 403 94
Bme1580I G_kGCm'C
Cuts at: 1790 2072 3046 3354 4911 6067 1790
Size: 282 974 308 1557 1156 2573
Fragments arranged by size:
2573 1557 1156 974 308 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6850
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4581 5539 442
Size: 416 53 83 399 24 3164 958 1753
Fragments arranged by size:
3164 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3419 5510 1239
Size: 2180 2091 2579
Fragments arranged by size:
2579 2180 2091
Bpu10I CC'TnA_GC
Cuts at: 4001 4001
Size: 6850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4975 5840 6138 4975
Size: 865 298 5687
Fragments arranged by size:
5687 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 2467 2965 5513 882
Size: 617 594 374 498 2548 2219
Fragments arranged by size:
2548 2219 617 594 498 374
BsaAI yAC'GTr
Cuts at: 493 1204 4641 493
Size: 711 3437 2702
Fragments arranged by size:
3437 2702 711
BsaBI GATnn'nnATC
Cuts at: 6844 6844
Size: 6850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2934 3214 4657 5039
Size: 53 83 186 936 1401 280 1443 382
Cuts at: 5039 5674 275
Size: 635 1451
Fragments arranged by size:
1451 1443 1401 936 635 382 280 186
83 53
BsaWI w'CCGG_w
Cuts at: 2239 2330 2985 4040 5283 5681 5955 6102 6513
Size: 91 655 1055 1243 398 274 147 411
Cuts at: 6513 2239
Size: 2576
Fragments arranged by size:
2576 1243 1055 655 411 398 274 147
91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 3458 3488 1178
Size: 30 165 30 663 30 1362 30 4540
Fragments arranged by size:
4540 1362 663 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2485 3074 3664 1186
Size: 1299 589 590 4372
Fragments arranged by size:
4372 1299 590 589
BseYI C'CCAG_C
Cuts at: 2017 2055 2077 2322 2564 2778 3747 6053 6549
Size: 38 22 245 242 214 969 2306 496
Cuts at: 6549 2017
Size: 2318
Fragments arranged by size:
2318 2306 969 496 245 242 214 38
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3050 1163
Size: 573 1314 4963
Fragments arranged by size:
4963 1314 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 3040 4300 5061 6089 1540
Size: 275 186 655 384 1260 761 1028 2301
Fragments arranged by size:
2301 1260 1028 761 655 384 275 186
BsiHKAI G_wGCw'C
Cuts at: 2404 3189 3624 4911 4996 6067 2404
Size: 785 435 1287 85 1071 3187
Fragments arranged by size:
3187 1287 1071 785 435 85
BsmI GAATG_Cn'
Cuts at: 4361 4454 4361
Size: 93 6757
BsmBI CGTCTCn'nnnn_
Cuts at: 3205 3933 3205
Size: 728 6122
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2404 2668 3046 3189 3354 3624
Size: 399 282 332 264 378 143 165 270
Cuts at: 3624 3744 3886 3937 4169 4911 4996 6067 1391
Size: 120 142 51 232 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 332 282 270
264 232 165 143 142 120 85 51
BspEI T'CCGG_A
Cuts at: 2330 6513 2330
Size: 4183 2667
BspHI T'CATG_A
Cuts at: 4739 6469 4739
Size: 1730 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 3072 3113 4205 844
Size: 1936 292 41 1092 3489
Fragments arranged by size:
3489 1936 1092 292 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2862 3096 4737 1394
Size: 429 1039 234 1641 3507
Fragments arranged by size:
3507 1641 1039 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 2584 5345 5519 65
Size: 1183 1336 2761 174 1396
Fragments arranged by size:
2761 1396 1336 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2239 3192 3630 4040 5494 5681 1700
Size: 110 429 953 438 410 1454 187 2869
Fragments arranged by size:
2869 1454 953 438 429 410 187 110
BsrGI T'GTAC_A
Cuts at: 96 3246 96
Size: 3150 3700
BssHII G'CGCG_C
Cuts at: 1925 3101 1925
Size: 1176 5674
BssSI C'ACGA_G
Cuts at: 4910 5922 4910
Size: 1012 5838
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3111 794
Size: 2317 4533
BstBI TT'CG_AA
Cuts at: 5654 5654
Size: 6850
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6850
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 2212 2393 2998 3613 2140
Size: 72 181 605 615 5377
Fragments arranged by size:
5377 615 605 181 72
BstYI r'GATC_y
Cuts at: 1972 2805 4180 4547 4932 4949 6401 6487 6499
Size: 833 1375 367 385 17 1452 86 12
Cuts at: 6499 6845 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1375 833 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2211 2656 3276 3383 3700 4050 513
Size: 552 1146 445 620 107 317 350 3313
Fragments arranged by size:
3313 1146 620 552 445 350 317 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3844 487
Size: 683 56 2618 3493
Fragments arranged by size:
3493 2618 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2687 2745 2959 4366 5160 5180 2100
Size: 587 58 214 1407 794 20 3770
Fragments arranged by size:
3770 1407 794 587 214 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6815
DraI TTT'AAA
Cuts at: 4154 4154
Size: 6850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5857 809
Size: 507 4541 1802
Fragments arranged by size:
4541 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2659 2730
Size: 54 1425 50 275 18 207 622 71
Cuts at: 2730 3009 3037 3198 3390 3421 3573 4297 5186
Size: 279 28 161 192 31 152 724 889
Cuts at: 5186 6815 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 724 622 279 275 207
192 161 152 71 54 50 43 31
28 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 4297 1537
Size: 275 1225 1260 4090
Fragments arranged by size:
4090 1260 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2301 3903 3925 4780 1353
Size: 812 136 1602 22 855 3423
Fragments arranged by size:
3423 1602 855 812 136 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3541 5437 5811 5957 387
Size: 1064 138 1952 1896 374 146 1280
Fragments arranged by size:
1952 1896 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3622 3622
Size: 6850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3419 4054 4913 5510 6297 6565 1239
Size: 393 1787 635 859 597 787 268 1524
Fragments arranged by size:
1787 1524 859 787 635 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2032 2487 2769 3043 3565 3893 3997 2032
Size: 455 282 274 522 328 104 4885
Fragments arranged by size:
4885 522 455 328 282 274 104
EcoRI G'AATT_C
Cuts at: 4159 4537 4159
Size: 378 6472
FspI TGC'GCA
Cuts at: 5356 5356
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 2425 3025 3733 5997 1678
Size: 747 600 708 2264 2531
Fragments arranged by size:
2531 2264 747 708 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2207 2239 2525
Size: 32 135 32 494 32 109 32 286
Cuts at: 2525 2557 3119 3151 4065 4097 5491 5523 5565
Size: 32 562 32 914 32 1394 32 42
Cuts at: 5565 5597 1373
Size: 32 2626
Fragments arranged by size:
2626 1394 914 562 494 286 135 109
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 4194 4440 669
Size: 988 281 820 1436 246 3079
Fragments arranged by size:
3079 1436 988 820 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 2158 4449 2158
Size: 2291 4559
MluI A'CGCG_T
Cuts at: 6726 6726
Size: 6850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 2637 2676 5964 6148 1305
Size: 1212 120 39 3288 184 2007
Fragments arranged by size:
3288 2007 1212 184 120 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 3011 3200 3392 3423
Size: 54 1425 343 207 972 189 192 31
Cuts at: 3423 3575 10
Size: 152 3285
Fragments arranged by size:
3285 1425 972 343 207 192 189 152
54 31
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2675 2872 3611 4808 5167 5326
Size: 648 1185 324 197 739 1197 359 159
Cuts at: 5326 518
Size: 2042
Fragments arranged by size:
2042 1197 1185 739 648 359 324 197
159
MspA1I CmG'CkG
Cuts at: 1606 2185 2196 2326 2371 2782 3522 4266 4943
Size: 579 11 130 45 411 740 744 677
Cuts at: 4943 6091 6336 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 744 740 677 579 411 245
130 45 11
NcoI C'CATG_G
Cuts at: 513 1065 2211 3276 513
Size: 552 1146 1065 4087
Fragments arranged by size:
4087 1146 1065 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6850
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6850
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NsiI A_TGCA'T
Cuts at: 2010 2286 2010
Size: 276 6574
NspI r_CATG'y
Cuts at: 1132 1229 2288 3494 3685 4208 5753 1132
Size: 97 1059 1206 191 523 1545 2229
Fragments arranged by size:
2229 1545 1206 1059 523 191 97
PasI CC'CwG_GG
Cuts at: 1348 2087 3346 1348
Size: 739 1259 4852
Fragments arranged by size:
4852 1259 739
PciI A'CATG_T
Cuts at: 5749 5749
Size: 6850
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 2707 3796 1108
Size: 1395 204 1089 4162
Fragments arranged by size:
4162 1395 1089 204
PfoI T'CCnGG_A
Cuts at: 2406 3624 2406
Size: 1218 5632
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 4919 4951 6432 6464 3908
Size: 32 979 32 1481 32 4294
Fragments arranged by size:
4294 1481 979 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2769 2032
Size: 737 6113
PshAI GACnn'nnGTC
Cuts at: 1613 2105 3078 1613
Size: 492 973 5385
Fragments arranged by size:
5385 973 492
PsiI TTA'TAA
Cuts at: 4420 4420
Size: 6850
PspOMI G'GGCC_C
Cuts at: 3042 3042
Size: 6850
PspXI vC'TCGA_Gb
Cuts at: 3885 3885
Size: 6850
PstI C_TGCA'G
Cuts at: 830 2429 2752 4109 4202 830
Size: 1599 323 1357 93 3478
Fragments arranged by size:
3478 1599 1357 323 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 2371 2782 3522 2371
Size: 411 740 5699
Fragments arranged by size:
5699 740 411
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 6850
SacI G_AGCT'C
Cuts at: 3624 3624
Size: 6850
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 3925 2165
Size: 1760 5090
SbfI CC_TGCA'GG
Cuts at: 4202 4202
Size: 6850
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SfcI C'TryA_G
Cuts at: 826 1045 2425 2748 3355 4105 4198 5333 6014
Size: 219 1380 323 607 750 93 1135 681
Cuts at: 6014 6205 826
Size: 191 1471
Fragments arranged by size:
1471 1380 1135 750 681 607 323 219
191 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2738 2738
Size: 6850
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 3332 4177 1872
Size: 1460 845 4545
Fragments arranged by size:
4545 1460 845
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3885 4954 5855 6117 820
Size: 197 926 1942 1069 901 262 1553
Fragments arranged by size:
1942 1553 1069 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 4208 1132
Size: 3076 3774
SrfI GCCC'GGGC
Cuts at: 3332 3332
Size: 6850
SspI AAT'ATT
Cuts at: 5 52 4774 5
Size: 47 4722 2081
Fragments arranged by size:
4722 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6850
StyI C'CwwG_G
Cuts at: 513 1065 2211 3276 4289 513
Size: 552 1146 1065 1013 3074
Fragments arranged by size:
3074 1146 1065 1013 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2095 2827 3496 4877 4894 5047 5232
Size: 1255 515 732 669 1381 17 153 185
Cuts at: 5232 5677 325
Size: 445 1498
Fragments arranged by size:
1498 1381 1255 732 669 515 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3246 5096 6808
Size: 274 80 33 51 1443 1269 1850 1712
Cuts at: 6808 96
Size: 138
Fragments arranged by size:
1850 1712 1443 1269 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6810 6842 6810
Size: 32 6818
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6850
XbaI T'CTAG_A
Cuts at: 4186 4186
Size: 6850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2140 2500 3343 2140
Size: 360 843 5647
Fragments arranged by size:
5647 843 360
XhoI C'TCGA_G
Cuts at: 1943 3885 1943
Size: 1942 4908
XmaI C'CCGG_G
Cuts at: 1870 3330 4175 1870
Size: 1460 845 4545
Fragments arranged by size:
4545 1460 845
XmnI GAAnn'nnTTC
Cuts at: 3923 4979 3923
Size: 1056 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4658 276
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI
SacII SanDI SexAI SfoI SwaI