Restriction map for
pFN21AE0034
(Circular) (Six-base) MAPSORT of: pFN21AE0034.seq Check: 8136 from: 1 to: 6193
pFN21AE0034 6193 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 3343 3536 1937
Size: 268 1138 193 4594
Fragments arranged by size:
4594 1138 268 193
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2906 4256 5640 5908 1632
Size: 483 791 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 791 483 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2730 1166
Size: 1564 4629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2182 2339 2770 3299 3502 3728 3880 5989
Size: 689 157 431 529 203 226 152 2109
Cuts at: 5989 1493
Size: 1697
Fragments arranged by size:
2109 1697 689 529 431 226 203 157
152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2554 3152 3518 1870
Size: 73 611 598 366 4545
Fragments arranged by size:
4545 611 598 366 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3514 4969 618
Size: 325 217 627 1727 1455 1842
Fragments arranged by size:
1842 1727 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3330 3512 1391
Size: 1939 182 4072
Fragments arranged by size:
4072 1939 182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 2952 928
Size: 971 645 408 4169
Fragments arranged by size:
4169 971 645 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BfrBI ATG'CAT
Cuts at: 2440 2440
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2479 4804 136
Size: 107 122 71 631 605 807 2325 1525
Fragments arranged by size:
2325 1525 807 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2359 6188 2359
Size: 3829 2364
BlpI GC'TnA_GC
Cuts at: 2366 2758 3219 3610 2366
Size: 392 461 391 4949
Fragments arranged by size:
4949 461 392 391
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 4882 442
Size: 416 53 83 399 24 2507 958 1753
Fragments arranged by size:
2507 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 3179 4853 1239
Size: 965 975 1674 2579
Fragments arranged by size:
2579 1674 975 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 4318 5183 5481 2131
Size: 2187 865 298 2843
Fragments arranged by size:
2843 2187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 3441 4856 882
Size: 617 515 1427 1415 2219
Fragments arranged by size:
2219 1427 1415 617 515
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 2871 6187 2871
Size: 3316 2877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2321 4626 5024 5298 5445 5856 2321
Size: 2305 398 274 147 411 2658
Fragments arranged by size:
2658 2305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2023 2053 1178
Size: 30 165 30 620 30 5318
Fragments arranged by size:
5318 620 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2722 2941 1186
Size: 858 678 219 4438
Fragments arranged by size:
4438 858 678 219
BseYI C'CCAG_C
Cuts at: 2445 2712 3329 5396 5892 2445
Size: 267 617 2067 496 2746
Fragments arranged by size:
2746 2067 617 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2856 1163
Size: 573 1120 4500
Fragments arranged by size:
4500 1120 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4254 4339 5410 4254
Size: 85 1071 5037
Fragments arranged by size:
5037 1071 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3330 3512 4254 4339 5410 1391
Size: 399 1540 182 742 85 1071 2174
Fragments arranged by size:
2174 1540 1071 742 399 182 85
BspEI T'CCGG_A
Cuts at: 2321 5856 2321
Size: 3535 2658
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3548 844
Size: 2704 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4837 5024 1700
Size: 110 3027 187 2869
Fragments arranged by size:
3027 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2801 96
Size: 2705 3488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2105 794
Size: 1311 4882
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2732 2732
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2359 3523 3890 4275 4292 5744 5830 5842
Size: 387 1164 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1164 387 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3344 3344
Size: 6193
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2704 487
Size: 683 56 1478 3976
Fragments arranged by size:
3976 1478 683 56
BtsI GCAGTG_nn'
Cuts at: 2844 3383 3709 4503 4523 2844
Size: 539 326 794 20 4514
Fragments arranged by size:
4514 794 539 326 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 2658 3497 2658
Size: 839 5354
DraIII CAC_nnn'GTG
Cuts at: 3179 3179
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2299 2734 3640
Size: 54 1425 50 275 18 469 435 906
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 906 889 469 435 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3039 3246 4123 1353
Size: 1686 207 877 3423
Fragments arranged by size:
3423 1686 877 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2204 2906 3179 4256 4853 5640
Size: 393 483 89 702 273 1077 597 787
Cuts at: 5640 5908 1239
Size: 268 1524
Fragments arranged by size:
1524 1077 787 702 597 483 393 273
268 89
EcoO109I rG'GnC_Cy
Cuts at: 2527 2527
Size: 6193
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
EcoRV GAT'ATC
Cuts at: 2695 2695
Size: 6193
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 2654 2686 4834
Size: 32 135 32 451 32 599 32 2148
Cuts at: 4834 4866 4908 4940 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2148 599 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2422 3139 748
Size: 1674 717 3802
Fragments arranged by size:
3802 1674 717
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 2678 3792 2678
Size: 1114 5079
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3194 5307 5491 1305
Size: 1889 2113 184 2007
Fragments arranged by size:
2113 2007 1889 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2301 2736 10
Size: 54 1425 343 469 435 3467
Fragments arranged by size:
3467 1425 469 435 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 4151 4510 4669 518
Size: 648 1564 1421 359 159 2042
Fragments arranged by size:
2042 1564 1421 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2730 2740 3609 4286 5434 5679 1606
Size: 1124 10 869 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 869 677 245 10
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 2442 2442
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3551 5096 1132
Size: 97 2322 1545 2229
Fragments arranged by size:
2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 1108
Size: 1382 4811
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3051 3763 3051
Size: 712 5481
PstI C_TGCA'G
Cuts at: 830 2399 3545 830
Size: 1569 1146 3478
Fragments arranged by size:
3478 1569 1146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2740 2740
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 2317 4441 6153 2317
Size: 2124 1712 2357
Fragments arranged by size:
2357 2124 1712
SfcI C'TryA_G
Cuts at: 826 1045 2395 3541 4676 5357 5548 826
Size: 219 1350 1146 1135 681 191 1471
Fragments arranged by size:
1471 1350 1146 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 2554 4297 5198 5460 820
Size: 197 926 167 444 1743 901 262 1553
Fragments arranged by size:
1743 1553 926 901 444 262 197 167
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 3026 3166 4117 5
Size: 47 2974 140 951 2081
Fragments arranged by size:
2974 2081 951 140 47
StuI AGG'CCT
Cuts at: 1517 2116 1517
Size: 599 5594
StyI C'CwwG_G
Cuts at: 513 1065 3461 3632 513
Size: 552 2396 171 3074
Fragments arranged by size:
3074 2396 552 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2131 2315 2801
Size: 274 80 33 51 1443 154 184 486
Cuts at: 2801 4439 6151 96
Size: 1638 1712 138
Fragments arranged by size:
1712 1638 1443 486 274 184 154 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 2041 3373 3529 2041
Size: 1332 156 4705
Fragments arranged by size:
4705 1332 156
XhoI C'TCGA_G
Cuts at: 1943 2554 1943
Size: 611 5582
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI