Restriction map for
pFN21AE0033
(Circular) (Six-base) MAPSORT of: pFN21AE0033.seq Check: 9979 from: 1 to: 6397
pFN21AE0033 6397 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3655 3655
Size: 6397
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4207 278
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3740 1937
Size: 1803 4594
Acc65I G'GTAC_C
Cuts at: 3718 3718
Size: 6397
AclI AA'CG_TT
Cuts at: 1563 4524 4897 1563
Size: 2961 373 3063
Fragments arranged by size:
3063 2961 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 4460 5844 6112 1632
Size: 591 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 591 268
AfeI AGC'GCT
Cuts at: 2059 2059
Size: 6397
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6200
AflIII A'CryG_T
Cuts at: 1203 3676 5296 6273 1203
Size: 2473 1620 977 1327
Fragments arranged by size:
2473 1620 1327 977
AgeI A'CCGG_T
Cuts at: 5228 5228
Size: 6397
AhdI GACnn_n'nnGTC
Cuts at: 5126 5126
Size: 6397
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 5067
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3039 3071 3039
Size: 32 6365
AlwNI CAG_nnn'CTG
Cuts at: 4140 5712 4140
Size: 1572 4825
ApaI G_GGCC'C
Cuts at: 3240 3240
Size: 6397
ApaLI G'TGCA_C
Cuts at: 4454 5610 4454
Size: 1156 5241
ApoI r'AATT_y
Cuts at: 1493 2415 2744 3137 3428 3706 3932 4084 6193
Size: 922 329 393 291 278 226 152 2109
Cuts at: 6193 1493
Size: 1697
Fragments arranged by size:
2109 1697 922 393 329 291 278 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3261 4951 160
Size: 872 2229 1690 1606
Fragments arranged by size:
2229 1690 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
AvaI C'yCGr_G
Cuts at: 1870 1943 3627 3722 1870
Size: 73 1684 95 4545
Fragments arranged by size:
4545 1684 95 73
BamHI G'GATC_C
Cuts at: 3727 4094 3727
Size: 367 6030
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3718 5173 618
Size: 325 217 627 1931 1455 1842
Fragments arranged by size:
1931 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2318 3240 3628 3716 1391
Size: 927 922 388 88 4072
Fragments arranged by size:
4072 927 922 388 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5426
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4588 4622 1903
Size: 34 2651 34 3678
Fragments arranged by size:
3678 2651 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4289 5499 4289
Size: 1210 5187
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 5008 136
Size: 107 122 71 631 605 397 2939 1525
Fragments arranged by size:
2939 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 2646 6392 2646
Size: 3746 2651
BlpI GC'TnA_GC
Cuts at: 2054 2976 3814 2054
Size: 922 838 4637
Fragments arranged by size:
4637 922 838
Bme1580I G_kGCm'C
Cuts at: 1790 3240 4458 5614 1790
Size: 1450 1218 1156 2573
Fragments arranged by size:
2573 1450 1218 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6397
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2719 3298 4128
Size: 416 53 83 399 24 1302 579 830
Cuts at: 4128 5086 442
Size: 958 1753
Fragments arranged by size:
1753 1302 958 830 579 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3099 5057 1239
Size: 1860 1958 2579
Fragments arranged by size:
2579 1958 1860
Bpu10I CC'TnA_GC
Cuts at: 3600 3600
Size: 6397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4522 5387 5685 4522
Size: 865 298 5234
Fragments arranged by size:
5234 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5060 882
Size: 617 3561 2219
Fragments arranged by size:
3561 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4188 493
Size: 711 2984 2702
Fragments arranged by size:
2984 2702 711
BsaBI GATnn'nnATC
Cuts at: 6391 6391
Size: 6397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4204 4586 5221 275
Size: 53 83 186 936 2671 382 635 1451
Fragments arranged by size:
2671 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4830 5228 5502 5649 6060 4830
Size: 398 274 147 411 5167
Fragments arranged by size:
5167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 3039 3069 3447
Size: 30 165 30 955 30 651 30 378
Cuts at: 3447 3477 1178
Size: 30 4098
Fragments arranged by size:
4098 955 651 378 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3463 1186
Size: 2277 4120
BseYI C'CCAG_C
Cuts at: 5600 6096 5600
Size: 496 5901
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3527 1163
Size: 573 1791 4033
Fragments arranged by size:
4033 1791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3847 4608 5636 1540
Size: 275 186 1846 761 1028 2301
Fragments arranged by size:
2301 1846 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4458 4543 5614 4458
Size: 85 1071 5241
Fragments arranged by size:
5241 1071 85
BsmI GAATG_Cn'
Cuts at: 2639 3022 3908 4001 2639
Size: 383 886 93 5035
Fragments arranged by size:
5035 886 383 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2318 3240 3628 3716 4458 4543 5614
Size: 399 528 922 388 88 742 85 1071
Cuts at: 5614 1391
Size: 2174
Fragments arranged by size:
2174 1071 922 742 528 399 388 88
85
BspEI T'CCGG_A
Cuts at: 6060 6060
Size: 6397
BspHI T'CATG_A
Cuts at: 2728 4286 6016 2728
Size: 1558 1730 3109
Fragments arranged by size:
3109 1730 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3655 3752 844
Size: 2811 97 3489
Fragments arranged by size:
3489 2811 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4284 1394
Size: 429 2461 3507
Fragments arranged by size:
3507 2461 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3444 4892 5066 65
Size: 1183 2196 1448 174 1396
Fragments arranged by size:
2196 1448 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5041 5228 1700
Size: 110 3231 187 2869
Fragments arranged by size:
3231 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2158 2357 3223 96
Size: 2062 199 866 3270
Fragments arranged by size:
3270 2062 866 199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6397
BssSI C'ACGA_G
Cuts at: 4457 5469 4457
Size: 1012 5385
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3278 3514 794
Size: 2484 236 3677
Fragments arranged by size:
3677 2484 236
BstBI TT'CG_AA
Cuts at: 5201 5201
Size: 6397
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6397
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 6397
BstYI r'GATC_y
Cuts at: 1972 2539 2646 3594 3727 4094 4479 4496 5948
Size: 567 107 948 133 367 385 17 1452
Cuts at: 5948 6034 6046 6392 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 948 567 385 367 346 133
107 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4906
Fragments arranged by size:
4906 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5658
Fragments arranged by size:
5658 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3048 3913 4707 4727 2025
Size: 1023 865 794 20 3695
Fragments arranged by size:
3695 1023 865 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 3662
Fragments arranged by size:
3662 2665 35 35
DraI TTT'AAA
Cuts at: 2223 3701 2223
Size: 1478 4919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3364 5404 809
Size: 507 2048 2040 1802
Fragments arranged by size:
2048 2040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 3844 4733
Size: 54 1425 50 275 18 240 1774 889
Cuts at: 4733 6362 8
Size: 1629 43
Fragments arranged by size:
1774 1629 1425 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3844 1537
Size: 275 2032 4090
Fragments arranged by size:
4090 2032 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4327 1353
Size: 2974 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4984 5358 5504 387
Size: 1064 138 3395 374 146 1280
Fragments arranged by size:
3395 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 3099 4460 5057 5844 6112 1239
Size: 393 591 876 1361 597 787 268 1524
Fragments arranged by size:
1524 1361 876 787 597 591 393 268
EcoRI G'AATT_C
Cuts at: 2415 3706 4084 2415
Size: 1291 378 4728
Fragments arranged by size:
4728 1291 378
EcoRV GAT'ATC
Cuts at: 3129 3129
Size: 6397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2542 3297 2539
Size: 3 755 5639
Fragments arranged by size:
5639 755 3
FspI TGC'GCA
Cuts at: 4903 4903
Size: 6397
HaeII r_GCGC'y
Cuts at: 1678 2061 5544 1678
Size: 383 3483 2531
Fragments arranged by size:
3483 2531 383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5038 5070 5112 5144 1373
Size: 32 135 32 3466 32 42 32 2626
Fragments arranged by size:
3466 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3741 3987 669
Size: 988 281 1803 246 3079
Fragments arranged by size:
3079 1803 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6397
HpaI GTT'AAC
Cuts at: 3987 3987
Size: 6397
KpnI G_GTAC'C
Cuts at: 3722 3722
Size: 6397
MfeI C'AATT_G
Cuts at: 3996 3996
Size: 6397
MluI A'CGCG_T
Cuts at: 6273 6273
Size: 6397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 3397 5511 5695 1305
Size: 844 1248 2114 184 2007
Fragments arranged by size:
2114 2007 1248 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4575
Fragments arranged by size:
4575 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 4355 4714 4873 518
Size: 648 912 2277 359 159 2042
Fragments arranged by size:
2277 2042 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 3813 4490 5638 5883 1606
Size: 447 1760 677 1148 245 2120
Fragments arranged by size:
2120 1760 1148 677 447 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4906
Fragments arranged by size:
4906 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6397
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6397
NotI GC'GGCC_GC
Cuts at: 3844 3844
Size: 6397
NspI r_CATG'y
Cuts at: 1132 1229 3680 3755 5300 1132
Size: 97 2451 75 1545 2229
Fragments arranged by size:
2451 2229 1545 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6397
PciI A'CATG_T
Cuts at: 3676 5296 3676
Size: 1620 4777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6397
PmeI GTTT'AAAC
Cuts at: 3701 3701
Size: 6397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3039 3071 4466 4498 5979 6011 3039
Size: 32 1395 32 1481 32 3425
Fragments arranged by size:
3425 1481 1395 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6397
PsiI TTA'TAA
Cuts at: 3967 3967
Size: 6397
PspOMI G'GGCC_C
Cuts at: 3236 3236
Size: 6397
PstI C_TGCA'G
Cuts at: 830 2102 3749 830
Size: 1272 1647 3478
Fragments arranged by size:
3478 1647 1272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6397
PvuII CAG'CTG
Cuts at: 2053 2053
Size: 6397
SalI G'TCGA_C
Cuts at: 1936 3739 1936
Size: 1803 4594
SbfI CC_TGCA'GG
Cuts at: 3749 3749
Size: 6397
ScaI AGT'ACT
Cuts at: 4645 6357 4645
Size: 1712 4685
SfcI C'TryA_G
Cuts at: 826 1045 2098 2758 3745 4880 5561 5752 826
Size: 219 1053 660 987 1135 681 191 1471
Fragments arranged by size:
1471 1135 1053 987 681 660 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6397
SmaI CCC'GGG
Cuts at: 1872 3724 1872
Size: 1852 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4501 5402 5664 820
Size: 197 926 2558 901 262 1553
Fragments arranged by size:
2558 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4188 493
Size: 3695 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6397
SphI G_CATG'C
Cuts at: 1132 3755 1132
Size: 2623 3774
SspI AAT'ATT
Cuts at: 5 52 4321 5
Size: 47 4269 2081
Fragments arranged by size:
4269 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6397
StyI C'CwwG_G
Cuts at: 513 1065 2004 3836 513
Size: 552 939 1832 3074
Fragments arranged by size:
3074 1832 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2730 4424 4441 4594 4779 5224 325
Size: 1255 1150 1694 17 153 185 445 1498
Fragments arranged by size:
1694 1498 1255 1150 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2158 2357 3223
Size: 274 80 33 51 1443 181 199 866
Cuts at: 3223 4643 6355 96
Size: 1420 1712 138
Fragments arranged by size:
1712 1443 1420 866 274 199 181 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 2608 2640 6357 6389 2416
Size: 32 160 32 3717 32 2424
Fragments arranged by size:
3717 2424 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6397
XbaI T'CTAG_A
Cuts at: 2771 3476 3733 2771
Size: 705 257 5435
Fragments arranged by size:
5435 705 257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6397
XmaI C'CCGG_G
Cuts at: 1870 3722 1870
Size: 1852 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 3054 4526 2522
Size: 532 1472 4393
Fragments arranged by size:
4393 1472 532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4205 276
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI