Restriction map for
pFN21AE0032
(Circular) (Six-base) MAPSORT of: pFN21AE0032.seq Check: 2331 from: 1 to: 6526
pFN21AE0032 6526 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2305 2305
Size: 6526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2482 3869 1937
Size: 545 1387 4594
Fragments arranged by size:
4594 1387 545
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2734 3758 3825 4589 5973 6241 1632
Size: 1102 1024 67 764 1384 268 1917
Fragments arranged by size:
1917 1384 1102 1024 764 268 67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 5425 6402 1203
Size: 4222 977 1327
Fragments arranged by size:
4222 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 2551 5255 2551
Size: 2704 3822
AleI CACnn'nnGTG
Cuts at: 1166 2498 2640 2894 3014 1166
Size: 1332 142 254 120 4678
Fragments arranged by size:
4678 1332 254 142 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2452 2484 2452
Size: 32 6494
AlwNI CAG_nnn'CTG
Cuts at: 2143 2320 3172 4269 5841 2143
Size: 177 852 1097 1572 2828
Fragments arranged by size:
2828 1572 1097 852 177
ApaI G_GGCC'C
Cuts at: 3490 3490
Size: 6526
ApaLI G'TGCA_C
Cuts at: 4583 5739 4583
Size: 1156 5370
ApoI r'AATT_y
Cuts at: 1493 2376 3230 3572 3835 4061 4213 6322 1493
Size: 883 854 342 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 883 854 342 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2131 3483 3794 3851 1870
Size: 73 188 1352 311 57 4545
Fragments arranged by size:
4545 1352 311 188 73 57
BamHI G'GATC_C
Cuts at: 2631 3391 3856 4223 2631
Size: 760 465 367 4934
Fragments arranged by size:
4934 760 465 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 3159 3847 5302 618
Size: 325 217 627 558 814 688 1455 1842
Fragments arranged by size:
1842 1455 814 688 627 558 325 217
BanII G_rGCy'C
Cuts at: 1391 2430 3490 3736 3795 3845 1391
Size: 1039 1060 246 59 50 4072
Fragments arranged by size:
4072 1060 1039 246 59 50
BbeI G_GCGC'C
Cuts at: 2349 2349
Size: 6526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2892 3139 928
Size: 971 993 247 4315
Fragments arranged by size:
4315 993 971 247
BbvCI CC'TCA_GC
Cuts at: 2573 3362 2573
Size: 789 5737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 4717 4751 1903
Size: 34 258 34 2488 34 3678
Fragments arranged by size:
3678 2488 258 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2604 3075 3302
Size: 107 122 71 631 605 932 471 227
Cuts at: 3302 3526 3670 5137 136
Size: 224 144 1467 1525
Fragments arranged by size:
1525 1467 932 631 605 471 227 224
144 122 107 71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 2225 2992 3596 3943 2225
Size: 767 604 347 4808
Fragments arranged by size:
4808 767 604 347
Bme1580I G_kGCm'C
Cuts at: 1790 3490 4587 5743 1790
Size: 1700 1097 1156 2573
Fragments arranged by size:
2573 1700 1156 1097
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2791 3628
Size: 416 53 83 399 24 658 716 837
Cuts at: 3628 4257 5215 442
Size: 629 958 1753
Fragments arranged by size:
1753 958 837 716 658 629 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2778 3737 5186 1239
Size: 1539 959 1449 2579
Fragments arranged by size:
2579 1539 1449 959
Bpu10I CC'TnA_GC
Cuts at: 2424 2573 3362 2424
Size: 149 789 5588
Fragments arranged by size:
5588 789 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3515 4651 5516 5814 3515
Size: 1136 865 298 4227
Fragments arranged by size:
4227 1136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 5189 882
Size: 617 675 3015 2219
Fragments arranged by size:
3015 2219 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 4317 493
Size: 711 3113 2702
Fragments arranged by size:
3113 2702 711
BsaBI GATnn'nnATC
Cuts at: 6520 6520
Size: 6526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 4333 4715 5350
Size: 53 83 186 936 813 1987 382 635
Cuts at: 5350 275
Size: 1451
Fragments arranged by size:
1987 1451 936 813 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2295 4959 5357 5631 5778 6189 2295
Size: 2664 398 274 147 411 2632
Fragments arranged by size:
2664 2632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 1178
Size: 30 165 30 1092 30 5179
Fragments arranged by size:
5179 1092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3104 3383 3602 3704 3812 1186
Size: 1918 279 219 102 108 3900
Fragments arranged by size:
3900 1918 279 219 108 102
BseYI C'CCAG_C
Cuts at: 2098 2320 2814 3521 5729 6225 2098
Size: 222 494 707 2208 496 2399
Fragments arranged by size:
2399 2208 707 496 494 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2790 3438 1163
Size: 573 453 601 648 4251
Fragments arranged by size:
4251 648 601 573 453
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3976 4737 5765 1540
Size: 275 186 1975 761 1028 2301
Fragments arranged by size:
2301 1975 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 4587 4672 5743 3795
Size: 792 85 1071 4578
Fragments arranged by size:
4578 1071 792 85
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 3490 3736 3795 3845 4587 4672
Size: 399 640 1060 246 59 50 742 85
Cuts at: 4672 5743 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1060 742 640 399 246 85
59 50
BspEI T'CCGG_A
Cuts at: 2295 6189 2295
Size: 3894 2632
BspHI T'CATG_A
Cuts at: 3255 4415 6145 3255
Size: 1160 1730 3636
Fragments arranged by size:
3636 1730 1160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2622 3366 3708 3881 844
Size: 1461 317 744 342 173 3489
Fragments arranged by size:
3489 1461 744 342 317 173
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 4413 1394
Size: 429 199 2391 3507
Fragments arranged by size:
3507 2391 429 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 2982 5021 5195 65
Size: 1183 1734 2039 174 1396
Fragments arranged by size:
2039 1734 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 2587 2617 2774 5170 5357 1700
Size: 110 356 421 30 157 2396 187 2869
Fragments arranged by size:
2869 2396 421 356 187 157 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 2890 4586 5598 2890
Size: 1696 1012 3818
Fragments arranged by size:
3818 1696 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3700 794
Size: 2906 3620
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 3353 1184
Size: 2169 4357
BstXI CCAn_nnnn'nTGG
Cuts at: 3016 3016
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2631 3261 3391 3783 3856 4223 4608 4625
Size: 659 630 130 392 73 367 385 17
Cuts at: 4625 6077 6163 6175 6521 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 659 630 392 385 367 346
130 86 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2635 2938 3009 513
Size: 552 1570 303 71 4030
Fragments arranged by size:
4030 1570 552 303 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5787
Fragments arranged by size:
5787 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 2505 4042 4836 4856 2116
Size: 389 1537 794 20 3786
Fragments arranged by size:
3786 1537 794 389 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 5533 809
Size: 507 698 3519 1802
Fragments arranged by size:
3519 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2472 3018 3671
Size: 54 1425 50 275 18 642 546 653
Cuts at: 3671 3973 4862 6491 8
Size: 302 889 1629 43
Fragments arranged by size:
1629 1425 889 653 642 546 302 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3579 3801 4456 1353
Size: 1586 640 222 655 3423
Fragments arranged by size:
3423 1586 655 640 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 3446 5113 5487 5633 387
Size: 1064 138 564 1293 1667 374 146 1280
Fragments arranged by size:
1667 1293 1280 1064 564 374 146 138
EcoICRI GAG'CTC
Cuts at: 3793 3793
Size: 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2734 2778 3737 3758 3825 4589 5186
Size: 393 1102 44 959 21 67 764 597
Cuts at: 5186 5973 6241 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1102 959 787 764 597 393 268
67 44 21
EcoO109I rG'GnC_Cy
Cuts at: 2488 3055 3487 2488
Size: 567 432 5527
Fragments arranged by size:
5527 567 432
EcoRI G'AATT_C
Cuts at: 2376 3230 3835 4213 2376
Size: 854 605 378 4689
Fragments arranged by size:
4689 854 605 378
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 2349 3773 5673 1678
Size: 671 1424 1900 2531
Fragments arranged by size:
2531 1900 1424 671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2990 3022 3325
Size: 32 135 32 965 32 421 32 303
Cuts at: 3325 3357 5167 5199 5241 5273 1373
Size: 32 1810 32 42 32 2626
Fragments arranged by size:
2626 1810 965 421 303 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3046 3220 3324 3870 4116 669
Size: 988 281 1108 174 104 546 246 3079
Fragments arranged by size:
3079 1108 988 546 281 246 174 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 3324 4116 3324
Size: 792 5734
KasI G'GCGC_C
Cuts at: 2345 2345
Size: 6526
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 5640 5824 1305
Size: 1263 3072 184 2007
Fragments arranged by size:
3072 2007 1263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3020 10
Size: 54 1425 343 1188 3516
Fragments arranged by size:
3516 1425 1188 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 2598 2640 2894 3014 3443 4484
Size: 648 1332 100 42 254 120 429 1041
Cuts at: 4484 4843 5002 518
Size: 359 159 2042
Fragments arranged by size:
2042 1332 1041 648 429 359 254 159
120 100 42
MspA1I CmG'CkG
Cuts at: 1606 2068 2407 2996 3525 3676 3706 3942 4619
Size: 462 339 589 529 151 30 236 677
Cuts at: 4619 5767 6012 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 529 462 339 245
236 151 30
NaeI GCC'GGC
Cuts at: 2168 2589 2168
Size: 421 6105
NarI GG'CG_CC
Cuts at: 2346 2346
Size: 6526
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5974
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NgoMIV G'CCGG_C
Cuts at: 2166 2587 2166
Size: 421 6105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 3884 5429 1132
Size: 97 2655 1545 2229
Fragments arranged by size:
2655 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6526
PfoI T'CCnGG_A
Cuts at: 3417 3786 3417
Size: 369 6157
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 6526
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 3486 3486
Size: 6526
PspXI vC'TCGA_Gb
Cuts at: 3794 3794
Size: 6526
PstI C_TGCA'G
Cuts at: 830 3315 3705 3878 830
Size: 2485 390 173 3478
Fragments arranged by size:
3478 2485 390 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2996 3525 3706 2996
Size: 529 181 5816
Fragments arranged by size:
5816 529 181
SacI G_AGCT'C
Cuts at: 3795 3795
Size: 6526
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 3617 4774 6486 3617
Size: 1157 1712 3657
Fragments arranged by size:
3657 1712 1157
SfcI C'TryA_G
Cuts at: 826 1045 2483 3311 3701 3713 3874 5009 5690
Size: 219 1438 828 390 12 161 1135 681
Cuts at: 5690 5881 826
Size: 191 1471
Fragments arranged by size:
1471 1438 1135 828 681 390 219 191
161 12
SfoI GGC'GCC
Cuts at: 2347 2347
Size: 6526
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2133 3485 3853 1872
Size: 261 1352 368 4545
Fragments arranged by size:
4545 1352 368 261
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3530 3794 4630 5531 5793 820
Size: 197 926 1587 264 836 901 262 1553
Fragments arranged by size:
1587 1553 926 901 836 264 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 3296 1517
Size: 1779 4747
StyI C'CwwG_G
Cuts at: 513 1065 2093 2694 2926 3249 3965 513
Size: 552 1028 601 232 323 716 3074
Fragments arranged by size:
3074 1028 716 601 552 323 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2135 2480 2723 4553 4570 4723
Size: 1255 470 85 345 243 1830 17 153
Cuts at: 4723 4908 5353 325
Size: 185 445 1498
Fragments arranged by size:
1830 1498 1255 470 445 345 243 185
153 85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3372 3495 3615
Size: 274 80 33 51 1443 1395 123 120
Cuts at: 3615 4772 6484 96
Size: 1157 1712 138
Fragments arranged by size:
1712 1443 1395 1157 274 138 123 120
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2623 2655 6486 6518 2623
Size: 32 3831 32 2631
Fragments arranged by size:
3831 2631 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2858 1316
Size: 1542 4984
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 3794 1943
Size: 1851 4675
XmaI C'CCGG_G
Cuts at: 1870 2131 3483 3851 1870
Size: 261 1352 368 4545
Fragments arranged by size:
4545 1352 368 261
XmnI GAAnn'nnTTC
Cuts at: 4655 4655
Size: 6526
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI