Restriction map for
pFN21AE0027
(Circular) (Six-base) MAPSORT of: pFN21AE0027.seq Check: 3363 from: 1 to: 6415
pFN21AE0027 6415 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2151 4225 278
Size: 53 83 186 936 615 2074 2468
Fragments arranged by size:
2468 2074 936 615 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 4478 5862 6130 1632
Size: 608 2238 1384 268 1917
Fragments arranged by size:
2238 1917 1384 608 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 3230 5314 6291 1203
Size: 2027 2084 977 1327
Fragments arranged by size:
2084 2027 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 2991 1166
Size: 1825 4590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2109 2892 2985 1184
Size: 925 783 93 4614
Fragments arranged by size:
4614 925 783 93
AlwNI CAG_nnn'CTG
Cuts at: 2493 2695 3026 3562 4158 5730 2493
Size: 202 331 536 596 1572 3178
Fragments arranged by size:
3178 1572 596 536 331 202
ApaI G_GGCC'C
Cuts at: 2020 2020
Size: 6415
ApaLI G'TGCA_C
Cuts at: 2867 4472 5628 2867
Size: 1605 1156 3654
Fragments arranged by size:
3654 1605 1156
ApoI r'AATT_y
Cuts at: 1493 3724 3950 4102 6211 1493
Size: 2231 226 152 2109 1697
Fragments arranged by size:
2231 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2128 2933 3740 1870
Size: 73 76 109 805 807 4545
Fragments arranged by size:
4545 807 805 109 76 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2606 2639 2606
Size: 33 6382
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3736 5191 618
Size: 325 217 627 1949 1455 1842
Fragments arranged by size:
1949 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2127 2237 2259 3734 1391
Size: 629 107 110 22 1475 4072
Fragments arranged by size:
4072 1475 629 110 107 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5444
BbvCI CC'TCA_GC
Cuts at: 3257 3257
Size: 6415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4307 5517 4307
Size: 1210 5205
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6415
BfrBI ATG'CAT
Cuts at: 3329 3329
Size: 6415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 5026 136
Size: 107 122 71 631 605 1330 2024 1525
Fragments arranged by size:
2024 1525 1330 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3083 3832 3083
Size: 749 5666
Bme1580I G_kGCm'C
Cuts at: 1790 2020 2144 2871 3532 4476 5632 1790
Size: 230 124 727 661 944 1156 2573
Fragments arranged by size:
2573 1156 944 727 661 230 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3238 3348 3512
Size: 416 53 83 399 24 1821 110 164
Cuts at: 3512 3676 4146 5104 442
Size: 164 470 958 1753
Fragments arranged by size:
1821 1753 958 470 416 399 164 164
110 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2534
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 2600 2657 3036 3126 3402 3648 5075
Size: 1177 184 57 379 90 276 246 1427
Cuts at: 5075 1239
Size: 2579
Fragments arranged by size:
2579 1427 1177 379 276 246 184 90
57
Bpu10I CC'TnA_GC
Cuts at: 3257 3257
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4540 5405 5703 3436
Size: 1104 865 298 4148
Fragments arranged by size:
4148 1104 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 2867 3573 5078 882
Size: 617 681 687 706 1505 2219
Fragments arranged by size:
2219 1505 706 687 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 2659 4206 493
Size: 711 1455 1547 2702
Fragments arranged by size:
2702 1547 1455 711
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 4222 4604 5239
Size: 53 83 186 936 615 2074 382 635
Cuts at: 5239 275
Size: 1451
Fragments arranged by size:
2074 1451 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2551 3489 3519 1178
Size: 30 165 30 1118 30 938 30 4074
Fragments arranged by size:
4074 1118 938 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3195 3420 1186
Size: 2009 225 4181
Fragments arranged by size:
4181 2009 225
BseYI C'CCAG_C
Cuts at: 2098 3606 5618 6114 2098
Size: 1508 2012 496 2399
Fragments arranged by size:
2399 2012 1508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2385 2690 3536 1163
Size: 573 423 226 305 846 4042
Fragments arranged by size:
4042 846 573 423 305 226
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 3865 4626 5654 1540
Size: 275 186 111 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2031 2237 2871 3639 4476 4561 5632 2031
Size: 206 634 768 837 85 1071 2814
Fragments arranged by size:
2814 1071 837 768 634 206 85
BsiWI C'GTAC_G
Cuts at: 2159 2159
Size: 6415
BsmI GAATG_Cn'
Cuts at: 3926 4019 3926
Size: 93 6322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2031 2127 2144 2237 2259 2871
Size: 399 230 11 96 17 93 22 612
Cuts at: 2871 3532 3639 3734 4476 4561 5632 1391
Size: 661 107 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 612 399 230 107
96 95 93 85 22 17 11
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 3387 4304 6034 3387
Size: 917 1730 3768
Fragments arranged by size:
3768 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 2712 2967 3770 844
Size: 1717 151 255 803 3489
Fragments arranged by size:
3489 1717 803 255 151
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4910 5084 65
Size: 1183 3662 174 1396
Fragments arranged by size:
3662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 5059 5246 1700
Size: 110 244 3005 187 2869
Fragments arranged by size:
3005 2869 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 2067 1925
Size: 142 6273
BssSI C'ACGA_G
Cuts at: 2864 3707 4475 5487 2864
Size: 843 768 1012 3792
Fragments arranged by size:
3792 1012 843 768
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6415
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstXI CCAn_nnnn'nTGG
Cuts at: 3619 3619
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 2215 2691 3745 4112 4497 4514 5966 6052
Size: 243 476 1054 367 385 17 1452 86
Cuts at: 6052 6064 6410 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1054 476 385 367 346 243
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3060 3191 513
Size: 552 939 1056 131 3737
Fragments arranged by size:
3737 1056 939 552 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2986 2998 3510 3931 4725 4745 2033
Size: 953 12 512 421 794 20 3703
Fragments arranged by size:
3703 953 794 512 421 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3202 5422 809
Size: 507 1886 2220 1802
Fragments arranged by size:
2220 1886 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2590 3573
Size: 54 1425 50 275 18 349 411 983
Cuts at: 3573 3862 4751 6380 8
Size: 289 889 1629 43
Fragments arranged by size:
1629 1425 983 889 411 349 289 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 3452 3547 4345 1353
Size: 1272 827 95 798 3423
Fragments arranged by size:
3423 1272 827 798 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5002 5376 5522 387
Size: 1064 138 3413 374 146 1280
Fragments arranged by size:
3413 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 6415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2416 2600 2657 3036 3126 3402
Size: 393 608 176 184 57 379 90 276
Cuts at: 3402 3648 4478 5075 5862 6130 1239
Size: 246 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 608 597 393 379 276
268 246 184 176 90 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 2243
Size: 6415
EcoO109I rG'GnC_Cy
Cuts at: 2016 2788 2016
Size: 772 5643
EcoRI G'AATT_C
Cuts at: 3724 4102 3724
Size: 378 6037
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 3607 5562 1678
Size: 1929 1955 2531
Fragments arranged by size:
2531 1955 1929
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2193 2225 2702 2734 3108
Size: 32 135 32 621 32 477 32 374
Cuts at: 3108 3140 5056 5088 5130 5162 1373
Size: 32 1916 32 42 32 2626
Fragments arranged by size:
2626 1916 621 477 374 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 5529 5713 1305
Size: 951 3273 184 2007
Fragments arranged by size:
3273 2007 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 3575 10
Size: 54 1425 343 760 983 2850
Fragments arranged by size:
2850 1425 983 760 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2537 2967 2991 3518 4373 4732
Size: 648 1044 327 430 24 527 855 359
Cuts at: 4732 4891 518
Size: 159 2042
Fragments arranged by size:
2042 1044 855 648 527 430 359 327
159 24
MspA1I CmG'CkG
Cuts at: 1606 3831 4508 5656 5901 1606
Size: 2225 677 1148 245 2120
Fragments arranged by size:
2225 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3060 3191 513
Size: 552 939 1056 131 3737
Fragments arranged by size:
3737 1056 939 552 131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NsiI A_TGCA'T
Cuts at: 3331 3331
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 3082 3213 3279 3333 3773 5318 1132
Size: 97 1853 131 66 54 440 1545 2229
Fragments arranged by size:
2229 1853 1545 440 131 97 66 54
PasI CC'CwG_GG
Cuts at: 1348 3299 1348
Size: 1951 4464
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3192 3250 3524 3562 1108
Size: 2084 58 274 38 3961
Fragments arranged by size:
3961 2084 274 58 38
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PspOMI G'GGCC_C
Cuts at: 2016 2016
Size: 6415
PstI C_TGCA'G
Cuts at: 830 2040 2993 3422 3461 3663 3767 830
Size: 1210 953 429 39 202 104 3478
Fragments arranged by size:
3478 1210 953 429 202 104 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 6415
SbfI CC_TGCA'GG
Cuts at: 3422 3767 3422
Size: 345 6070
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SexAI A'CCwGG_T
Cuts at: 2797 2797
Size: 6415
SfcI C'TryA_G
Cuts at: 826 1045 2036 2260 2472 2989 3006 3418 3457
Size: 219 991 224 212 517 17 412 39
Cuts at: 3457 3659 3763 4898 5579 5770 826
Size: 202 104 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 517 412 224 219
212 202 191 104 39 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 2021 2130 3742 1872
Size: 149 109 1612 4545
Fragments arranged by size:
4545 1612 149 109
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3451 4519 5420 5682 820
Size: 197 926 1508 1068 901 262 1553
Fragments arranged by size:
1553 1508 1068 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3279 3773 1132
Size: 2147 494 3774
Fragments arranged by size:
3774 2147 494
SspI AAT'ATT
Cuts at: 5 52 2762 4339 5
Size: 47 2710 1577 2081
Fragments arranged by size:
2710 2081 1577 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2593 2907 3060 3135 3191
Size: 552 939 114 475 314 153 75 56
Cuts at: 3191 3492 3570 3854 513
Size: 301 78 284 3074
Fragments arranged by size:
3074 939 552 475 314 301 284 153
114 78 75 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 2900 4442 4459 4612 4797 5242
Size: 1255 546 774 1542 17 153 185 445
Cuts at: 5242 325
Size: 1498
Fragments arranged by size:
1542 1498 1255 774 546 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 4661 6373
Size: 274 80 33 51 1443 573 2111 1712
Cuts at: 6373 96
Size: 138
Fragments arranged by size:
2111 1712 1443 573 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3489 3521 6375 6407 3489
Size: 32 2854 32 3497
Fragments arranged by size:
3497 2854 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3020 1316
Size: 1704 4711
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3067
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 2019 2128 3740 1870
Size: 149 109 1612 4545
Fragments arranged by size:
4545 1612 149 109
XmnI GAAnn'nnTTC
Cuts at: 4544 4544
Size: 6415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2149 4223 276
Size: 53 83 186 936 615 2074 2468
Fragments arranged by size:
2468 2074 936 615 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI