Restriction map for
pFN21AE0025
(Circular) (Six-base) MAPSORT of: pFN21AE0025.seq Check: 5072 from: 1 to: 6427
pFN21AE0025 6427 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4490 5874 6142 1632
Size: 2858 1384 268 1917
Fragments arranged by size:
2858 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 4170 5742 4170
Size: 1572 4855
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 2498 3067 3093 3736 3962 4114 6223 1493
Size: 1005 569 26 643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1005 643 569 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2282 2989 3030 3636 3752 1870
Size: 73 222 117 707 41 606 116 4545
Fragments arranged by size:
4545 707 606 222 117 116 73 41
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 3748 5203 618
Size: 325 217 627 382 1579 1455 1842
Fragments arranged by size:
1842 1579 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2727 3643 3746 1391
Size: 1336 916 103 4072
Fragments arranged by size:
4072 1336 916 103
BbeI G_GCGC'C
Cuts at: 2173 2173
Size: 6427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3491 3525 4618 4652 1903
Size: 34 1554 34 1093 34 3678
Fragments arranged by size:
3678 1554 1093 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4319 5529 3326
Size: 993 1210 4224
Fragments arranged by size:
4224 1210 993
BclI T'GATC_A
Cuts at: 1548 2599 3112 1548
Size: 1051 513 4863
Fragments arranged by size:
4863 1051 513
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5038 136
Size: 107 122 71 631 605 3366 1525
Fragments arranged by size:
3366 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 4488 5644 1790
Size: 2698 1156 2573
Fragments arranged by size:
2698 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 3379 4158
Size: 416 53 83 399 24 765 1197 779
Cuts at: 4158 5116 442
Size: 958 1753
Fragments arranged by size:
1753 1197 958 779 765 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3662
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2835 5087 1239
Size: 1596 2252 2579
Fragments arranged by size:
2579 2252 1596
Bpu10I CC'TnA_GC
Cuts at: 3414 3414
Size: 6427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4552 5417 5715 4552
Size: 865 298 5264
Fragments arranged by size:
5264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 2896 5090 882
Size: 617 666 731 2194 2219
Fragments arranged by size:
2219 2194 731 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 4234 4616 5251
Size: 53 83 186 936 637 2064 382 635
Cuts at: 5251 275
Size: 1451
Fragments arranged by size:
2064 1451 936 637 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2703 4860 5258 5532 5679 6090 2703
Size: 2157 398 274 147 411 3040
Fragments arranged by size:
3040 2157 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 2385 2415 2538
Size: 30 165 30 672 30 280 30 123
Cuts at: 2538 2568 1178
Size: 30 5037
Fragments arranged by size:
5037 672 280 165 123 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 3066 1186
Size: 967 913 4547
Fragments arranged by size:
4547 967 913
BseYI C'CCAG_C
Cuts at: 2116 5630 6126 2116
Size: 3514 496 2417
Fragments arranged by size:
3514 2417 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 1163
Size: 573 942 4912
Fragments arranged by size:
4912 942 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 4488 4573 5644 2130
Size: 2358 85 1071 2913
Fragments arranged by size:
2913 2358 1071 85
BsmI GAATG_Cn'
Cuts at: 2947 3938 4031 2947
Size: 991 93 5343
Fragments arranged by size:
5343 991 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2727 3643 3746 4488 4573 5644
Size: 399 340 597 916 103 742 85 1071
Cuts at: 5644 1391
Size: 2174
Fragments arranged by size:
2174 1071 916 742 597 399 340 103
85
BspEI T'CCGG_A
Cuts at: 2703 6090 2703
Size: 3387 3040
BspHI T'CATG_A
Cuts at: 2596 2694 4316 6046 2596
Size: 98 1622 1730 2977
Fragments arranged by size:
2977 1730 1622 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3782 844
Size: 2938 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 4314 1394
Size: 429 358 2133 3507
Fragments arranged by size:
3507 2133 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 2022 4922 5096 65
Size: 1183 774 2900 174 1396
Fragments arranged by size:
2900 1396 1183 774 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5071 5258 1700
Size: 110 3261 187 2869
Fragments arranged by size:
3261 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2348 3666 96
Size: 2252 1318 2857
Fragments arranged by size:
2857 2252 1318
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2966 3590 794
Size: 2172 624 3631
Fragments arranged by size:
3631 2172 624
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstYI r'GATC_y
Cuts at: 1972 3757 4124 4509 4526 5978 6064 6076 6422
Size: 1785 367 385 17 1452 86 12 346
Cuts at: 6422 1972
Size: 1977
Fragments arranged by size:
1977 1785 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 3943 4737 4757 3943
Size: 794 20 5613
Fragments arranged by size:
5613 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3478 3513 529
Size: 35 2914 35 3443
Fragments arranged by size:
3443 2914 35 35
DraI TTT'AAA
Cuts at: 2956 3280 3731 2956
Size: 324 451 5652
Fragments arranged by size:
5652 451 324
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 3874 4763
Size: 54 1425 50 275 18 243 1801 889
Cuts at: 4763 6392 8
Size: 1629 43
Fragments arranged by size:
1801 1629 1425 889 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2204 2662 4357 1353
Size: 782 69 458 1695 3423
Fragments arranged by size:
3423 1695 782 458 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2835 4490 5087 5874 6142 1239
Size: 393 1203 1655 597 787 268 1524
Fragments arranged by size:
1655 1524 1203 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3413 3413
Size: 6427
EcoO109I rG'GnC_Cy
Cuts at: 2086 3515 2086
Size: 1429 4998
EcoRI G'AATT_C
Cuts at: 2498 3736 4114 2498
Size: 1238 378 4811
Fragments arranged by size:
4811 1238 378
EcoRV GAT'ATC
Cuts at: 2821 2821
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2173 5574 1678
Size: 495 3401 2531
Fragments arranged by size:
3401 2531 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2584 2616 5068
Size: 32 135 32 626 32 354 32 2452
Cuts at: 5068 5100 5142 5174 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2452 626 354 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3043 3771 4017 669
Size: 988 281 1105 728 246 3079
Fragments arranged by size:
3079 1105 988 728 281 246
HindIII A'AGCT_T
Cuts at: 748 2326 3175 748
Size: 1578 849 4000
Fragments arranged by size:
4000 1578 849
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KasI G'GCGC_C
Cuts at: 2169 2169
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2473 5541 5725 1305
Size: 1168 3068 184 2007
Fragments arranged by size:
3068 2007 1168 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 10
Size: 54 1425 343 243 4362
Fragments arranged by size:
4362 1425 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4385 4744 4903 518
Size: 648 3219 359 159 2042
Fragments arranged by size:
3219 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2342 2423 3843 4520 5668 5913 1606
Size: 533 203 81 1420 677 1148 245 2120
Fragments arranged by size:
2120 1420 1148 677 533 245 203 81
NarI GG'CG_CC
Cuts at: 2170 2170
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5875
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3785 5330 1132
Size: 97 2556 1545 2229
Fragments arranged by size:
2556 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3254 1108
Size: 2146 4281
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 3515 2086
Size: 1429 4998
PshAI GACnn'nnGTC
Cuts at: 1613 2538 1613
Size: 925 5502
PsiI TTA'TAA
Cuts at: 2046 3997 2046
Size: 1951 4476
PspXI vC'TCGA_Gb
Cuts at: 2282 3030 3636 2282
Size: 748 606 5073
Fragments arranged by size:
5073 748 606
PstI C_TGCA'G
Cuts at: 830 2508 3779 830
Size: 1678 1271 3478
Fragments arranged by size:
3478 1678 1271
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2342 2423 2342
Size: 81 6346
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SapI GCTCTTCn'nnn_
Cuts at: 2135 2662 2135
Size: 527 5900
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 3036 4675 6387 3036
Size: 1639 1712 3076
Fragments arranged by size:
3076 1712 1639
SfcI C'TryA_G
Cuts at: 826 1045 2504 3775 4910 5591 5782 826
Size: 219 1459 1271 1135 681 191 1471
Fragments arranged by size:
1471 1459 1271 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2171 2171
Size: 6427
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 3030 3636 4531 5432 5694
Size: 197 926 339 748 606 895 901 262
Cuts at: 5694 820
Size: 1553
Fragments arranged by size:
1553 926 901 895 748 606 339 262
197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 2314 3687 4351 5
Size: 47 2262 1373 664 2081
Fragments arranged by size:
2262 2081 1373 664 47
StuI AGG'CCT
Cuts at: 1517 2220 1517
Size: 703 5724
StyI C'CwwG_G
Cuts at: 513 1065 2536 3062 3620 3866 513
Size: 552 1471 526 558 246 3074
Fragments arranged by size:
3074 1471 558 552 526 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4454 4471 4624 4809 5254 325
Size: 1255 2874 17 153 185 445 1498
Fragments arranged by size:
2874 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2348 2796 3034
Size: 274 80 33 51 1443 371 448 238
Cuts at: 3034 3666 4673 6385 96
Size: 632 1007 1712 138
Fragments arranged by size:
1712 1443 1007 632 448 371 274 238
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2385 2417 2433 2465 2538 2570 6387 6419 2385
Size: 32 16 32 73 32 3817 32 2393
Fragments arranged by size:
3817 2393 73 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 2282 3030 3636 1943
Size: 339 748 606 4734
Fragments arranged by size:
4734 748 606 339
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI