Restriction map for
pFN21AE0018
(Circular) (Six-base) MAPSORT of: pFN21AE0018.seq Check: 2760 from: 1 to: 7273
pFN21AE0018 7273 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3708 3708
Size: 7273
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5083 278
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 4616 1937
Size: 349 2330 4594
Fragments arranged by size:
4594 2330 349
Acc65I G'GTAC_C
Cuts at: 4594 4594
Size: 7273
AclI AA'CG_TT
Cuts at: 1563 3009 3018 5400 5773 1563
Size: 1446 9 2382 373 3063
Fragments arranged by size:
3063 2382 1446 373 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3800 3815 5336 6720 6988 1632
Size: 2168 15 1521 1384 268 1917
Fragments arranged by size:
2168 1917 1521 1384 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7076
AflIII A'CryG_T
Cuts at: 1203 4455 4570 6172 7149 1203
Size: 3252 115 1602 977 1327
Fragments arranged by size:
3252 1602 1327 977 115
AgeI A'CCGG_T
Cuts at: 6104 6104
Size: 7273
AhdI GACnn_n'nnGTC
Cuts at: 6002 6002
Size: 7273
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 7241
AlwNI CAG_nnn'CTG
Cuts at: 3663 5016 6588 3663
Size: 1353 1572 4348
Fragments arranged by size:
4348 1572 1353
ApaI G_GGCC'C
Cuts at: 2433 2433
Size: 7273
ApaLI G'TGCA_C
Cuts at: 5330 6486 5330
Size: 1156 6117
ApoI r'AATT_y
Cuts at: 1493 2237 2697 3136 3244 3970 4351 4582 4808
Size: 744 460 439 108 726 381 231 226
Cuts at: 4808 4960 7069 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 744 726 460 439 381 231
226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 3738 5827 160
Size: 872 2706 2089 1606
Fragments arranged by size:
2706 2089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
AvaI C'yCGr_G
Cuts at: 1870 1943 3390 4598 1870
Size: 73 1447 1208 4545
Fragments arranged by size:
4545 1447 1208 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4296 4329 4296
Size: 33 7240
BamHI G'GATC_C
Cuts at: 4603 4970 4603
Size: 367 6906
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4594 6049 618
Size: 325 217 627 2807 1455 1842
Fragments arranged by size:
2807 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2433 4592 1391
Size: 1042 2159 4072
Fragments arranged by size:
4072 2159 1042
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3167 3412 3591 928
Size: 971 1268 245 179 4610
Fragments arranged by size:
4610 1268 971 245 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5464 5498 1903
Size: 34 3527 34 3678
Fragments arranged by size:
3678 3527 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3556 5165 6375 3556
Size: 1609 1210 4454
Fragments arranged by size:
4454 1609 1210
BclI T'GATC_A
Cuts at: 1548 3471 1548
Size: 1923 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5884 136
Size: 107 122 71 631 605 4212 1525
Fragments arranged by size:
4212 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2662 7268 2662
Size: 4606 2667
BlpI GC'TnA_GC
Cuts at: 2837 4690 2837
Size: 1853 5420
Bme1580I G_kGCm'C
Cuts at: 1790 2433 5334 6490 1790
Size: 643 2901 1156 2573
Fragments arranged by size:
2901 2573 1156 643
BmgBI CAC'GTC
Cuts at: 1318 4456 1318
Size: 3138 4135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3598 5004
Size: 416 53 83 399 24 1206 975 1406
Cuts at: 5004 5962 442
Size: 958 1753
Fragments arranged by size:
1753 1406 1206 975 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2586 5933 1239
Size: 1347 3347 2579
Fragments arranged by size:
3347 2579 1347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5398 6263 6561 5398
Size: 865 298 6110
Fragments arranged by size:
6110 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 2707 2765 5936 882
Size: 617 624 584 58 3171 2219
Fragments arranged by size:
3171 2219 624 617 584 58
BsaAI yAC'GTr
Cuts at: 493 1204 5064 493
Size: 711 3860 2702
Fragments arranged by size:
3860 2702 711
BsaBI GATnn'nnATC
Cuts at: 7267 7267
Size: 7273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3417 5080 5462 6097
Size: 53 83 186 936 1884 1663 382 635
Cuts at: 6097 275
Size: 1451
Fragments arranged by size:
1884 1663 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3410 5706 6104 6378 6525 6936 3410
Size: 2296 398 274 147 411 3747
Fragments arranged by size:
3747 2296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2071 2101 4442 4472 1178
Size: 30 165 30 668 30 2341 30 3979
Fragments arranged by size:
3979 2341 668 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7273
BseYI C'CCAG_C
Cuts at: 2035 3283 6476 6972 2035
Size: 1248 3193 496 2336
Fragments arranged by size:
3193 2336 1248 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3831 1163
Size: 573 2095 4605
Fragments arranged by size:
4605 2095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4723 5484 6512 1540
Size: 275 186 2722 761 1028 2301
Fragments arranged by size:
2722 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4058 5334 5419 6490 4058
Size: 1276 85 1071 4841
Fragments arranged by size:
4841 1276 1071 85
BsmI GAATG_Cn'
Cuts at: 2163 3200 4784 4877 2163
Size: 1037 1584 93 4559
Fragments arranged by size:
4559 1584 1037 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2433 4058 4592 5334 5419 6490 1391
Size: 399 643 1625 534 742 85 1071 2174
Fragments arranged by size:
2174 1625 1071 742 643 534 399 85
BspEI T'CCGG_A
Cuts at: 6936 6936
Size: 7273
BspHI T'CATG_A
Cuts at: 5162 6892 5162
Size: 1730 5543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 3107 3708 4628 844
Size: 2086 177 601 920 3489
Fragments arranged by size:
3489 2086 920 601 177
BsrBI CCG'CTC
Cuts at: 1394 1823 4298 5160 1394
Size: 429 2475 862 3507
Fragments arranged by size:
3507 2475 862 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5768 5942 65
Size: 1183 4520 174 1396
Fragments arranged by size:
4520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5917 6104 1700
Size: 110 4107 187 2869
Fragments arranged by size:
4107 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7273
BssSI C'ACGA_G
Cuts at: 3535 5333 6345 3535
Size: 1798 1012 4463
Fragments arranged by size:
4463 1798 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7273
BstBI TT'CG_AA
Cuts at: 6077 6077
Size: 7273
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7273
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2150
Size: 7273
BstYI r'GATC_y
Cuts at: 1972 2662 4603 4970 5355 5372 6824 6910 6922
Size: 690 1941 367 385 17 1452 86 12
Cuts at: 6922 7268 1972
Size: 346 1977
Fragments arranged by size:
1977 1941 1452 690 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2091 2438 2091
Size: 347 6926
BtgI C'CryG_G
Cuts at: 513 1065 2004 2751 2988 513
Size: 552 939 747 237 4798
Fragments arranged by size:
4798 939 747 552 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6534
Fragments arranged by size:
6534 683 56
BtsI GCAGTG_nn'
Cuts at: 3769 4247 4789 5583 5603 3769
Size: 478 542 794 20 5439
Fragments arranged by size:
5439 794 542 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2377 2412 529
Size: 35 1813 35 5390
Fragments arranged by size:
5390 1813 35 35
DraI TTT'AAA
Cuts at: 4577 4577
Size: 7273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2282 6280 809
Size: 507 966 3998 1802
Fragments arranged by size:
3998 1802 966 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4290 4720 5609
Size: 54 1425 50 275 18 2460 430 889
Cuts at: 5609 7238 8
Size: 1629 43
Fragments arranged by size:
2460 1629 1425 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4720 1537
Size: 275 2908 4090
Fragments arranged by size:
4090 2908 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3255 3699 5203 1353
Size: 1451 451 444 1504 3423
Fragments arranged by size:
3423 1504 1451 451 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2709 5860 6234 6380 387
Size: 1064 138 1120 3151 374 146 1280
Fragments arranged by size:
3151 1280 1120 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2586 3800 3815 5336 5933 6720 6988
Size: 393 954 1214 15 1521 597 787 268
Cuts at: 6988 1239
Size: 1524
Fragments arranged by size:
1524 1521 1214 954 787 597 393 268
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2437 2437
Size: 7273
EcoO109I rG'GnC_Cy
Cuts at: 2430 2430
Size: 7273
EcoRI G'AATT_C
Cuts at: 2697 3136 4582 4960 2697
Size: 439 1446 378 5010
Fragments arranged by size:
5010 1446 439 378
EcoRV GAT'ATC
Cuts at: 3154 3154
Size: 7273
FspI TGC'GCA
Cuts at: 5779 5779
Size: 7273
HaeII r_GCGC'y
Cuts at: 1678 6420 1678
Size: 4742 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2706 2732 2738 2764 3179
Size: 32 135 32 1134 26 6 26 415
Cuts at: 3179 3211 3384 3416 5914 5946 5988 6020 1373
Size: 32 173 32 2498 32 42 32 2626
Fragments arranged by size:
2626 2498 1134 415 173 135 42 32
32 32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4617 4863 669
Size: 988 281 2679 246 3079
Fragments arranged by size:
3079 2679 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7273
HpaI GTT'AAC
Cuts at: 4863 4863
Size: 7273
KpnI G_GTAC'C
Cuts at: 4598 4598
Size: 7273
MfeI C'AATT_G
Cuts at: 4872 4872
Size: 7273
MluI A'CGCG_T
Cuts at: 7149 7149
Size: 7273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2528 2766 3073 3412 3418 3429 4086 6387
Size: 1223 238 307 339 6 11 657 2301
Cuts at: 6387 6571 1305
Size: 184 2007
Fragments arranged by size:
2301 2007 1223 657 339 307 238 184
11 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4292 10
Size: 54 1425 343 2460 2991
Fragments arranged by size:
2991 2460 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3277 4530 5231 5590 5749 518
Size: 648 2111 1253 701 359 159 2042
Fragments arranged by size:
2111 2042 1253 701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2953 4689 5366 6514 6759 1606
Size: 1347 1736 677 1148 245 2120
Fragments arranged by size:
2120 1736 1347 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2751 513
Size: 552 939 747 5035
Fragments arranged by size:
5035 939 747 552
NdeI CA'TA_TG
Cuts at: 387 2254 3931 387
Size: 1867 1677 3729
Fragments arranged by size:
3729 1867 1677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7273
NotI GC'GGCC_GC
Cuts at: 4720 4720
Size: 7273
NspI r_CATG'y
Cuts at: 1132 1229 3377 4574 4631 6176 1132
Size: 97 2148 1197 57 1545 2229
Fragments arranged by size:
2229 2148 1545 1197 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7273
PciI A'CATG_T
Cuts at: 4570 6172 4570
Size: 1602 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7273
PmeI GTTT'AAAC
Cuts at: 4577 4577
Size: 7273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5342 5374 6855 6887 5342
Size: 32 1481 32 5728
Fragments arranged by size:
5728 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7273
PsiI TTA'TAA
Cuts at: 4843 4843
Size: 7273
PspOMI G'GGCC_C
Cuts at: 2429 2429
Size: 7273
PstI C_TGCA'G
Cuts at: 830 3705 4242 4625 830
Size: 2875 537 383 3478
Fragments arranged by size:
3478 2875 537 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7273
PvuII CAG'CTG
Cuts at: 2953 2953
Size: 7273
SalI G'TCGA_C
Cuts at: 1936 4615 1936
Size: 2679 4594
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 7273
SbfI CC_TGCA'GG
Cuts at: 4625 4625
Size: 7273
ScaI AGT'ACT
Cuts at: 4430 5521 7233 4430
Size: 1091 1712 4470
Fragments arranged by size:
4470 1712 1091
SexAI A'CCwGG_T
Cuts at: 2520 2520
Size: 7273
SfcI C'TryA_G
Cuts at: 826 1045 3701 4038 4238 4621 5756 6437 6628
Size: 219 2656 337 200 383 1135 681 191
Cuts at: 6628 826
Size: 1471
Fragments arranged by size:
2656 1471 1135 681 383 337 219 200
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7273
SmaI CCC'GGG
Cuts at: 1872 4600 1872
Size: 2728 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5377 6278 6540 820
Size: 197 926 3434 901 262 1553
Fragments arranged by size:
3434 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5064 493
Size: 4571 2702
SpeI A'CTAG_T
Cuts at: 152 2176 152
Size: 2024 5249
SphI G_CATG'C
Cuts at: 1132 3377 4631 1132
Size: 2245 1254 3774
Fragments arranged by size:
3774 2245 1254
SspI AAT'ATT
Cuts at: 5 52 5197 5
Size: 47 5145 2081
Fragments arranged by size:
5145 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 2751 4712 513
Size: 552 939 639 108 1961 3074
Fragments arranged by size:
3074 1961 939 639 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2721 3275 3797 5300 5317 5470 5655
Size: 1255 1141 554 522 1503 17 153 185
Cuts at: 5655 6100 325
Size: 445 1498
Fragments arranged by size:
1503 1498 1255 1141 554 522 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 2828 3088
Size: 274 80 33 51 1443 654 197 260
Cuts at: 3088 4428 5519 7231 96
Size: 1340 1091 1712 138
Fragments arranged by size:
1712 1443 1340 1091 654 274 260 197
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 7233 7265 2071
Size: 32 5130 32 2079
Fragments arranged by size:
5130 2079 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7273
XbaI T'CTAG_A
Cuts at: 3937 4300 4609 3937
Size: 363 309 6601
Fragments arranged by size:
6601 363 309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2782 4489 2782
Size: 1707 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7273
XmaI C'CCGG_G
Cuts at: 1870 4598 1870
Size: 2728 4545
XmnI GAAnn'nnTTC
Cuts at: 3710 4444 5402 3710
Size: 734 958 5581
Fragments arranged by size:
5581 958 734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5081 276
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI