Restriction map for
pFN21AE0016
(Circular) (Six-base) MAPSORT of: pFN21AE0016.seq Check: 1618 from: 1 to: 6928
pFN21AE0016 6928 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2379 4738 278
Size: 53 83 186 936 843 2359 2468
Fragments arranged by size:
2468 2359 936 843 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2224 4271 1937
Size: 287 2047 4594
Fragments arranged by size:
4594 2047 287
Acc65I G'GTAC_C
Cuts at: 4249 4249
Size: 6928
AclI AA'CG_TT
Cuts at: 1563 5055 5428 1563
Size: 3492 373 3063
Fragments arranged by size:
3492 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2800 3150 3936 4038 4991 6375 6643 1632
Size: 1168 350 786 102 953 1384 268 1917
Fragments arranged by size:
1917 1384 1168 953 786 350 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6731
AflIII A'CryG_T
Cuts at: 1203 5827 6804 1203
Size: 4624 977 1327
Fragments arranged by size:
4624 1327 977
AgeI A'CCGG_T
Cuts at: 5759 5759
Size: 6928
AhdI GACnn_n'nnGTC
Cuts at: 5657 5657
Size: 6928
AleI CACnn'nnGTG
Cuts at: 1166 2903 1166
Size: 1737 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6928
AlwNI CAG_nnn'CTG
Cuts at: 3060 4671 6243 3060
Size: 1611 1572 3745
Fragments arranged by size:
3745 1611 1572
ApaI G_GGCC'C
Cuts at: 2888 2888
Size: 6928
ApaLI G'TGCA_C
Cuts at: 2486 4985 6141 2486
Size: 2499 1156 3273
Fragments arranged by size:
3273 2499 1156
ApoI r'AATT_y
Cuts at: 1493 2726 4237 4463 4615 6724 1493
Size: 1233 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 1233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3944 5482 160
Size: 872 2912 1538 1606
Fragments arranged by size:
2912 1606 1538 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
AvaI C'yCGr_G
Cuts at: 1870 1943 2299 3773 4253 1870
Size: 73 356 1474 480 4545
Fragments arranged by size:
4545 1474 480 356 73
BamHI G'GATC_C
Cuts at: 3150 4258 4625 3150
Size: 1108 367 5453
Fragments arranged by size:
5453 1108 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4249 5704 618
Size: 325 217 627 2462 1455 1842
Fragments arranged by size:
2462 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2888 3298 4247 1391
Size: 1497 410 949 4072
Fragments arranged by size:
4072 1497 949 410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 3138 928
Size: 971 472 767 4718
Fragments arranged by size:
4718 971 767 472
BbvCI CC'TCA_GC
Cuts at: 3609 3609
Size: 6928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2483 2517 5119 5153 1903
Size: 34 546 34 2602 34 3678
Fragments arranged by size:
3678 2602 546 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4820 6030 4820
Size: 1210 5718
BclI T'GATC_A
Cuts at: 1548 3955 1548
Size: 2407 4521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 3102 5539
Size: 107 122 71 631 605 772 658 2437
Cuts at: 5539 136
Size: 1525
Fragments arranged by size:
2437 1525 772 658 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3790 6923 3790
Size: 3133 3795
BlpI GC'TnA_GC
Cuts at: 4345 4345
Size: 6928
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2539 2882 2888 4989 6145 1790
Size: 700 49 343 6 2101 1156 2573
Fragments arranged by size:
2573 2101 1156 700 343 49 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6928
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4659 5617 442
Size: 416 53 83 399 24 3242 958 1753
Fragments arranged by size:
3242 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2859 2887 3082 5588 1239
Size: 1620 28 195 2506 2579
Fragments arranged by size:
2579 2506 1620 195 28
Bpu10I CC'TnA_GC
Cuts at: 2795 3299 3450 3609 2795
Size: 504 151 159 6114
Fragments arranged by size:
6114 504 159 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4016 5053 5918 6216 4016
Size: 1037 865 298 4728
Fragments arranged by size:
4728 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5591 882
Size: 617 4092 2219
Fragments arranged by size:
4092 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2860 3805 4719 493
Size: 711 1125 531 945 914 2702
Fragments arranged by size:
2702 1125 945 914 711 531
BsaBI GATnn'nnATC
Cuts at: 2028 3795 6922 2028
Size: 1767 3127 2034
Fragments arranged by size:
3127 2034 1767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2376 2447 3778 4735
Size: 53 83 186 936 843 71 1331 957
Cuts at: 4735 5117 5752 275
Size: 382 635 1451
Fragments arranged by size:
1451 1331 957 936 843 635 382 186
83 71 53
BsaWI w'CCGG_w
Cuts at: 3723 5361 5759 6033 6180 6591 3723
Size: 1638 398 274 147 411 4060
Fragments arranged by size:
4060 1638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2115 2145 2903 2933 3605
Size: 30 165 30 712 30 758 30 672
Cuts at: 3605 3635 1178
Size: 30 4471
Fragments arranged by size:
4471 758 712 672 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2801 3626 3875 1186
Size: 1615 825 249 4239
Fragments arranged by size:
4239 1615 825 249
BseYI C'CCAG_C
Cuts at: 2686 2846 3254 3343 3988 6131 6627 2686
Size: 160 408 89 645 2143 496 2987
Fragments arranged by size:
2987 2143 645 496 408 160 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 2414 2541 3701 1163
Size: 573 594 84 127 1160 4390
Fragments arranged by size:
4390 1160 594 573 127 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2518 4378 5139 6167 1540
Size: 275 186 517 1860 761 1028 2301
Fragments arranged by size:
2301 1860 1028 761 517 275 186
BsiHKAI G_wGCw'C
Cuts at: 2490 3128 3427 3855 4989 5074 6145 2490
Size: 638 299 428 1134 85 1071 3273
Fragments arranged by size:
3273 1134 1071 638 428 299 85
BsmI GAATG_Cn'
Cuts at: 2605 2983 3823 4439 4532 2605
Size: 378 840 616 93 5001
Fragments arranged by size:
5001 840 616 378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2539 2882 2888 3128 3298 3427
Size: 399 700 49 343 6 240 170 129
Cuts at: 3427 3855 4247 4989 5074 6145 1391
Size: 428 392 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 700 428 399 392 343
240 170 129 85 49 6
BspEI T'CCGG_A
Cuts at: 6591 6591
Size: 6928
BspHI T'CATG_A
Cuts at: 2206 4817 6547 2206
Size: 2611 1730 2587
Fragments arranged by size:
2611 2587 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4283 844
Size: 3439 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4815 1394
Size: 429 2992 3507
Fragments arranged by size:
3507 2992 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3327 5423 5597 65
Size: 1183 2079 2096 174 1396
Fragments arranged by size:
2096 2079 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2256 2890 5572 5759 1700
Size: 110 446 634 2682 187 2869
Fragments arranged by size:
2869 2682 634 446 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6928
BssSI C'ACGA_G
Cuts at: 4988 6000 4988
Size: 1012 5916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6928
BstBI TT'CG_AA
Cuts at: 5732 5732
Size: 6928
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6928
BstYI r'GATC_y
Cuts at: 1972 3150 3790 4258 4625 5010 5027 6479 6565
Size: 1178 640 468 367 385 17 1452 86
Cuts at: 6565 6577 6923 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1178 640 468 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 4069 4155 513
Size: 552 939 312 1753 86 3286
Fragments arranged by size:
3286 1753 939 552 312 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6189
Fragments arranged by size:
6189 683 56
BtsI GCAGTG_nn'
Cuts at: 2655 4444 5238 5258 2655
Size: 1789 794 20 4325
Fragments arranged by size:
4325 1789 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6893
DraI TTT'AAA
Cuts at: 2694 4232 2694
Size: 1538 5390
DraIII CAC_nnn'GTG
Cuts at: 2317 2496 2317
Size: 179 6749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5935 809
Size: 507 4619 1802
Fragments arranged by size:
4619 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2457 2515
Size: 54 1425 50 275 18 177 450 58
Cuts at: 2515 2652 3136 4116 4375 5264 6893 8
Size: 137 484 980 259 889 1629 43
Fragments arranged by size:
1629 1425 980 889 484 450 275 259
177 137 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2515 4375 1537
Size: 275 703 1860 4090
Fragments arranged by size:
4090 1860 703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3922 4858 1353
Size: 1466 1103 936 3423
Fragments arranged by size:
3423 1466 1103 936
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3412 5515 5889 6035 387
Size: 1064 138 1823 2103 374 146 1280
Fragments arranged by size:
2103 1823 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2800 2859 2887 3082 3150 3936 4038
Size: 393 1168 59 28 195 68 786 102
Cuts at: 4038 4991 5588 6375 6643 1239
Size: 953 597 787 268 1524
Fragments arranged by size:
1524 1168 953 787 786 597 393 268
195 102 68 59 28
EcoO109I rG'GnC_Cy
Cuts at: 2167 2194 2806 2167
Size: 27 612 6289
Fragments arranged by size:
6289 612 27
EcoRI G'AATT_C
Cuts at: 4237 4615 4237
Size: 378 6550
FspI TGC'GCA
Cuts at: 3383 5434 3383
Size: 2051 4877
HaeII r_GCGC'y
Cuts at: 1678 6075 1678
Size: 4397 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2366 2398 3142
Size: 32 135 32 543 32 219 32 744
Cuts at: 3142 3174 3605 3637 5569 5601 5643 5675 1373
Size: 32 431 32 1932 32 42 32 2626
Fragments arranged by size:
2626 1932 744 543 431 219 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2335 4272 4518 669
Size: 988 281 220 177 1937 246 3079
Fragments arranged by size:
3079 1937 988 281 246 220 177
HindIII A'AGCT_T
Cuts at: 748 4020 748
Size: 3272 3656
HpaI GTT'AAC
Cuts at: 2158 4518 2158
Size: 2360 4568
KpnI G_GTAC'C
Cuts at: 4253 4253
Size: 6928
MfeI C'AATT_G
Cuts at: 4527 4527
Size: 6928
MluI A'CGCG_T
Cuts at: 6804 6804
Size: 6928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 3904 6042 6226 1305
Size: 690 1909 2138 184 2007
Fragments arranged by size:
2138 2007 1909 690 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 4118 10
Size: 54 1425 343 822 1464 2820
Fragments arranged by size:
2820 1464 1425 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2714 2903 2939 3807 4886 5245
Size: 648 843 705 189 36 868 1079 359
Cuts at: 5245 5404 518
Size: 159 2042
Fragments arranged by size:
2042 1079 868 843 705 648 359 189
159 36
MspA1I CmG'CkG
Cuts at: 1606 3335 3387 3503 3656 4344 5021 6169 6414
Size: 1729 52 116 153 688 677 1148 245
Cuts at: 6414 1606
Size: 2120
Fragments arranged by size:
2120 1729 1148 688 677 245 153 116
52
NaeI GCC'GGC
Cuts at: 2892 2892
Size: 6928
NcoI C'CATG_G
Cuts at: 513 1065 2004 4069 513
Size: 552 939 2065 3372
Fragments arranged by size:
3372 2065 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6928
NgoMIV G'CCGG_C
Cuts at: 2890 2890
Size: 6928
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6928
NotI GC'GGCC_GC
Cuts at: 4375 4375
Size: 6928
NspI r_CATG'y
Cuts at: 1132 1229 2794 2938 4286 5831 1132
Size: 97 1565 144 1348 1545 2229
Fragments arranged by size:
2229 1565 1545 1348 144 97
PasI CC'CwG_GG
Cuts at: 1348 2188 2442 1348
Size: 840 254 5834
Fragments arranged by size:
5834 840 254
PciI A'CATG_T
Cuts at: 5827 5827
Size: 6928
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 2619 1108
Size: 1209 302 5417
Fragments arranged by size:
5417 1209 302
PmeI GTTT'AAAC
Cuts at: 4232 4232
Size: 6928
PmlI CAC'GTG
Cuts at: 2329 3805 2329
Size: 1476 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4997 5029 6510 6542 4997
Size: 32 1481 32 5383
Fragments arranged by size:
5383 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 2194 2167
Size: 27 6901
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6928
PsiI TTA'TAA
Cuts at: 4498 4498
Size: 6928
PspOMI G'GGCC_C
Cuts at: 2884 2884
Size: 6928
PstI C_TGCA'G
Cuts at: 830 2406 4280 830
Size: 1576 1874 3478
Fragments arranged by size:
3478 1874 1576
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6928
PvuII CAG'CTG
Cuts at: 3503 3503
Size: 6928
SalI G'TCGA_C
Cuts at: 1936 4270 1936
Size: 2334 4594
SbfI CC_TGCA'GG
Cuts at: 4280 4280
Size: 6928
ScaI AGT'ACT
Cuts at: 5176 6888 5176
Size: 1712 5216
SfcI C'TryA_G
Cuts at: 826 1045 2402 3599 4276 5411 6092 6283 826
Size: 219 1357 1197 677 1135 681 191 1471
Fragments arranged by size:
1471 1357 1197 1135 681 677 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6928
SmaI CCC'GGG
Cuts at: 1872 4255 1872
Size: 2383 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4031 5032 5933 6195 820
Size: 197 926 2088 1001 901 262 1553
Fragments arranged by size:
2088 1553 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4719 493
Size: 4226 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6928
SphI G_CATG'C
Cuts at: 1132 2794 2938 4286 1132
Size: 1662 144 1348 3774
Fragments arranged by size:
3774 1662 1348 144
SspI AAT'ATT
Cuts at: 5 52 4852 5
Size: 47 4800 2081
Fragments arranged by size:
4800 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6928
StyI C'CwwG_G
Cuts at: 513 1065 2004 2560 4069 4367 513
Size: 552 939 556 1509 298 3074
Fragments arranged by size:
3074 1509 939 556 552 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 4955 4972 5125 5310 5755 325
Size: 1255 1031 2344 17 153 185 445 1498
Fragments arranged by size:
2344 1498 1255 1031 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 5174 6886
Size: 274 80 33 51 1443 429 2768 1712
Cuts at: 6886 96
Size: 138
Fragments arranged by size:
2768 1712 1443 429 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6888 6920 6888
Size: 32 6896
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 1316
Size: 813 6115
XbaI T'CTAG_A
Cuts at: 4108 4264 4108
Size: 156 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6928
XmaI C'CCGG_G
Cuts at: 1870 4253 1870
Size: 2383 4545
XmnI GAAnn'nnTTC
Cuts at: 5057 5057
Size: 6928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2377 4736 276
Size: 53 83 186 936 843 2359 2468
Fragments arranged by size:
2468 2359 936 843 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI