Restriction map for
pFN21AE0015
(Circular) (Six-base) MAPSORT of: pFN21AE0015.seq Check: 9730 from: 1 to: 6658
pFN21AE0015 6658 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4001 1937
Size: 2064 4594
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 3957 4785 5158 1563
Size: 2394 828 373 3063
Fragments arranged by size:
3063 2394 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2442 2608 2621 2793 3081 3126 3138
Size: 645 165 166 13 172 288 45 12
Cuts at: 3138 4721 6105 6373 1632
Size: 1583 1384 268 1917
Fragments arranged by size:
1917 1583 1384 645 288 268 172 166
165 45 13 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6461
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 2028 5387 2028
Size: 3359 3299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AlwNI CAG_nnn'CTG
Cuts at: 3104 3548 4401 5973 3104
Size: 444 853 1572 3789
Fragments arranged by size:
3789 1572 853 444
ApaI G_GGCC'C
Cuts at: 3597 3597
Size: 6658
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 2054 2390 3028 3773 3967 4193 4345 6454
Size: 561 336 638 745 194 226 152 2109
Cuts at: 6454 1493
Size: 1697
Fragments arranged by size:
2109 1697 745 638 561 336 226 194
152
AseI AT'TA_AT
Cuts at: 160 1032 5212 160
Size: 872 4180 1606
Fragments arranged by size:
4180 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 3945 3983 1870
Size: 73 2002 38 4545
Fragments arranged by size:
4545 2002 73 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3543 3576 3543
Size: 33 6625
BamHI G'GATC_C
Cuts at: 3988 4355 3988
Size: 367 6291
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3854 3979 5434 618
Size: 325 217 627 2067 125 1455 1842
Fragments arranged by size:
2067 1842 1455 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 3597 3952 3977 1391
Size: 2206 355 25 4072
Fragments arranged by size:
4072 2206 355 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3126 3523 3714 928
Size: 971 1227 397 191 3872
Fragments arranged by size:
3872 1227 971 397 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4849 4883 1903
Size: 34 50 34 2828 34 3678
Fragments arranged by size:
3678 2828 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4550 5760 4550
Size: 1210 5448
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5269 136
Size: 107 122 71 631 605 3597 1525
Fragments arranged by size:
3597 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2768 3605 6653 2768
Size: 837 3048 2773
Fragments arranged by size:
3048 2773 837
BlpI GC'TnA_GC
Cuts at: 4075 4075
Size: 6658
Bme1580I G_kGCm'C
Cuts at: 1790 3597 4719 5875 1790
Size: 1807 1122 1156 2573
Fragments arranged by size:
2573 1807 1156 1122
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4389 5347 442
Size: 416 53 83 399 24 2972 958 1753
Fragments arranged by size:
2972 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2664 2979 2664
Size: 315 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3699 3893 5318 1239
Size: 2460 194 1425 2579
Fragments arranged by size:
2579 2460 1425 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3276 4783 5648 5946 3276
Size: 1507 865 298 3988
Fragments arranged by size:
3988 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4449 493
Size: 711 3245 2702
Fragments arranged by size:
3245 2702 711
BsaBI GATnn'nnATC
Cuts at: 2946 6652 2946
Size: 3706 2952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4465 4847 5482 275
Size: 53 83 186 936 2932 382 635 1451
Fragments arranged by size:
2932 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5091 5489 5763 5910 6321 5091
Size: 398 274 147 411 5428
Fragments arranged by size:
5428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6433
Fragments arranged by size:
6433 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 2721 2955 1186
Size: 1286 249 234 4889
Fragments arranged by size:
4889 1286 249 234
BseYI C'CCAG_C
Cuts at: 5861 6357 5861
Size: 496 6162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6085
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4108 4869 5897 1540
Size: 275 186 2107 761 1028 2301
Fragments arranged by size:
2301 2107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4719 4804 5875 4719
Size: 85 1071 5502
Fragments arranged by size:
5502 1071 85
BsmI GAATG_Cn'
Cuts at: 2500 4169 4262 2500
Size: 1669 93 4896
Fragments arranged by size:
4896 1669 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3597 3952 3977 4719 4804 5875 1391
Size: 399 1807 355 25 742 85 1071 2174
Fragments arranged by size:
2174 1807 1071 742 399 355 85 25
BspEI T'CCGG_A
Cuts at: 6321 6321
Size: 6658
BspHI T'CATG_A
Cuts at: 4547 6277 4547
Size: 1730 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4013 844
Size: 3169 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4545 1394
Size: 429 2722 3507
Fragments arranged by size:
3507 2722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2345 5153 5327 65
Size: 1183 1097 2808 174 1396
Fragments arranged by size:
2808 1396 1183 1097 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5302 5489 1700
Size: 110 3492 187 2869
Fragments arranged by size:
3492 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 3460 5462 3460
Size: 2002 4656
BstEII G'GTnAC_C
Cuts at: 1184 2504 1184
Size: 1320 5338
BstYI r'GATC_y
Cuts at: 1972 2768 3296 3605 3988 4355 4740 4757 6209
Size: 796 528 309 383 367 385 17 1452
Cuts at: 6209 6295 6307 6653 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 796 528 385 383 367 346
309 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5919
Fragments arranged by size:
5919 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 4174 4968 4988 3058
Size: 1116 794 20 4728
Fragments arranged by size:
4728 1116 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2155 2190 529
Size: 35 1591 35 4997
Fragments arranged by size:
4997 1591 35 35
DraI TTT'AAA
Cuts at: 3214 3962 3214
Size: 748 5910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3895 3917 4105
Size: 54 1425 50 275 18 2065 22 188
Cuts at: 4105 4994 6623 8
Size: 889 1629 43
Fragments arranged by size:
2065 1629 1425 889 275 188 54 50
43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 4105 1537
Size: 275 2293 4090
Fragments arranged by size:
4090 2293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2775 3792 4588 1353
Size: 1422 1017 796 3423
Fragments arranged by size:
3423 1422 1017 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5245 5619 5765 387
Size: 1064 138 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2442 2608 2621 2793 3081 3126
Size: 393 645 165 166 13 172 288 45
Cuts at: 3126 3138 3699 3893 4721 5318 6105 6373 1239
Size: 12 561 194 828 597 787 268 1524
Fragments arranged by size:
1524 828 787 645 597 561 393 288
268 194 172 166 165 45 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2736 2736
Size: 6658
EcoO109I rG'GnC_Cy
Cuts at: 2726 2785 3254 3527 3545 2726
Size: 59 469 273 18 5839
Fragments arranged by size:
5839 469 273 59 18
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3111 3111
Size: 6658
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 5805 1678
Size: 4127 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 2130 2162 3359
Size: 32 135 32 442 32 84 32 1197
Cuts at: 3359 3391 5299 5331 5373 5405 1373
Size: 32 1908 32 42 32 2626
Fragments arranged by size:
2626 1908 1197 442 135 84 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2541 4002 4248 669
Size: 988 281 187 416 1461 246 3079
Fragments arranged by size:
3079 1461 988 416 281 246 187
HindIII A'AGCT_T
Cuts at: 748 2221 3396 748
Size: 1473 1175 4010
Fragments arranged by size:
4010 1473 1175
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5772 5956 1305
Size: 4467 184 2007
Fragments arranged by size:
4467 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3897 10
Size: 54 1425 343 2065 2771
Fragments arranged by size:
2771 2065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 3201 3429 4616 4975 5134 518
Size: 648 1399 636 228 1187 359 159 2042
Fragments arranged by size:
2042 1399 1187 648 636 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 3557 4074 4751 5899 6144 1606
Size: 1951 517 677 1148 245 2120
Fragments arranged by size:
2120 1951 1148 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 4016 5561 1132
Size: 97 2787 1545 2229
Fragments arranged by size:
2787 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6658
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2726 2785 3254 3527 3545 2726
Size: 59 469 273 18 5839
Fragments arranged by size:
5839 469 273 59 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6658
PsiI TTA'TAA
Cuts at: 3309 4228 3309
Size: 919 5739
PspOMI G'GGCC_C
Cuts at: 3593 3593
Size: 6658
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3154 3186 3154
Size: 32 6626
PstI C_TGCA'G
Cuts at: 830 4010 830
Size: 3180 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
PvuII CAG'CTG
Cuts at: 3557 3557
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 3091 4906 6618 3091
Size: 1815 1712 3131
Fragments arranged by size:
3131 1815 1712
SfcI C'TryA_G
Cuts at: 826 1045 2572 3300 4006 5141 5822 6013 826
Size: 219 1527 728 706 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 728 706 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3947 3985 1872
Size: 2075 38 4545
Fragments arranged by size:
4545 2075 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3291 4762 5663 5925 820
Size: 197 926 1348 1471 901 262 1553
Fragments arranged by size:
1553 1471 1348 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 2248 3579 4582 5
Size: 47 2196 1331 1003 2081
Fragments arranged by size:
2196 2081 1331 1003 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 4097 513
Size: 552 3032 3074
Fragments arranged by size:
3074 3032 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4685 4702 4855 5040 5485 325
Size: 1255 3105 17 153 185 445 1498
Fragments arranged by size:
3105 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3089 4904 6616
Size: 274 80 33 51 1443 1112 1815 1712
Cuts at: 6616 96
Size: 138
Fragments arranged by size:
1815 1712 1443 1112 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6618 6650 6618
Size: 32 6626
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 3149 3994 3149
Size: 845 5813
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 3945 3983 1870
Size: 2075 38 4545
Fragments arranged by size:
4545 2075 38
XmnI GAAnn'nnTTC
Cuts at: 4787 4787
Size: 6658
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI