Restriction map for
pFN21AE0012
(Circular) (Six-base) MAPSORT of: pFN21AE0012.seq Check: 9923 from: 1 to: 6268
pFN21AE0012 6268 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3611 1937
Size: 1674 4594
Acc65I G'GTAC_C
Cuts at: 2080 3589 2080
Size: 1509 4759
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2801 3089 4331 5715 5983 1632
Size: 567 602 288 1242 1384 268 1917
Fragments arranged by size:
1917 1384 1242 602 567 288 268
AflII C'TTAA_G
Cuts at: 820 1017 2189 820
Size: 197 1172 4899
Fragments arranged by size:
4899 1172 197
AflIII A'CryG_T
Cuts at: 1203 2932 5167 6144 1203
Size: 1729 2235 977 1327
Fragments arranged by size:
2235 1729 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2003
Size: 32 6236
AlwNI CAG_nnn'CTG
Cuts at: 4011 5583 4011
Size: 1572 4696
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 3362 3434 3448 3577 3803 3955 6064 1493
Size: 1869 72 14 129 226 152 2109 1697
Fragments arranged by size:
2109 1869 1697 226 152 129 72 14
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3593 1870
Size: 73 1650 4545
Fragments arranged by size:
4545 1650 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2973 3006 2973
Size: 33 6235
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 3589 5044 618
Size: 325 217 627 293 1509 1455 1842
Fragments arranged by size:
1842 1509 1455 627 325 293 217
BanII G_rGCy'C
Cuts at: 1391 3587 1391
Size: 2196 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3095 928
Size: 971 1196 4101
Fragments arranged by size:
4101 1196 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4459 4493 1903
Size: 34 50 34 2438 34 3678
Fragments arranged by size:
3678 2438 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 2329 2596 1548
Size: 781 267 5220
Fragments arranged by size:
5220 781 267
BfrBI ATG'CAT
Cuts at: 2226 2226
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2382 2926 4879
Size: 107 122 71 631 605 710 544 1953
Cuts at: 4879 136
Size: 1525
Fragments arranged by size:
1953 1525 710 631 605 544 122 107
71
BglII A'GATC_T
Cuts at: 3178 6263 3178
Size: 3085 3183
BlpI GC'TnA_GC
Cuts at: 2666 3685 2666
Size: 1019 5249
Bme1580I G_kGCm'C
Cuts at: 1790 2922 4329 5485 1790
Size: 1132 1407 1156 2573
Fragments arranged by size:
2573 1407 1156 1132
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2017 3249 3999
Size: 416 53 83 399 24 600 1232 750
Cuts at: 3999 4957 442
Size: 958 1753
Fragments arranged by size:
1753 1232 958 750 600 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4928 1239
Size: 3689 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 4393 5258 5556 2927
Size: 1466 865 298 3639
Fragments arranged by size:
3639 1466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4931 882
Size: 617 3432 2219
Fragments arranged by size:
3432 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 3062 6262 3062
Size: 3200 3068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4701 5099 5373 5520 5931 4701
Size: 398 274 147 411 5038
Fragments arranged by size:
5038 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3105 3135 1178
Size: 30 165 30 1702 30 4311
Fragments arranged by size:
4311 1702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 5013
BseYI C'CCAG_C
Cuts at: 5471 5967 5471
Size: 496 5772
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5695
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 2378 3779 3872 2378
Size: 1401 93 4774
Fragments arranged by size:
4774 1401 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2922 3587 4329 4414 5485 1391
Size: 399 1132 665 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 742 665 399 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 2866 4157 5887 2866
Size: 1291 1730 3247
Fragments arranged by size:
3247 1730 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2410 3623 844
Size: 1307 259 1213 3489
Fragments arranged by size:
3489 1307 1213 259
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2544 4763 4937 65
Size: 1183 1296 2219 174 1396
Fragments arranged by size:
2219 1396 1296 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2140 3178 3549 3598 3965 4350 4367 5819
Size: 168 1038 371 49 367 385 17 1452
Cuts at: 5819 5905 5917 6263 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1038 385 371 367 346 168
86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 3138 3138
Size: 6268
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 3784 4578 4598 3784
Size: 794 20 5454
Fragments arranged by size:
5454 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 2088 3151 3340 3572 2088
Size: 1063 189 232 4784
Fragments arranged by size:
4784 1063 232 189
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 5275 809
Size: 507 1221 2738 1802
Fragments arranged by size:
2738 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2770 3715
Size: 54 1425 50 275 18 561 379 945
Cuts at: 3715 4604 6233 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 945 889 561 379 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 3429 4198 1353
Size: 1733 343 769 3423
Fragments arranged by size:
3423 1733 769 343
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4855 5229 5375 387
Size: 1064 138 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2801 3089 4331 4928 5715 5983
Size: 393 567 602 288 1242 597 787 268
Cuts at: 5983 1239
Size: 1524
Fragments arranged by size:
1524 1242 787 602 597 567 393 288
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2955 2955
Size: 6268
EcoO109I rG'GnC_Cy
Cuts at: 2495 2495
Size: 6268
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
EcoRV GAT'ATC
Cuts at: 2572 2572
Size: 6268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2204 2458 2204
Size: 254 6014
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 5415 1678
Size: 3737 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4909 4941 4983 5015 1373
Size: 32 135 32 3337 32 42 32 2626
Fragments arranged by size:
3337 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3612 3858 669
Size: 988 281 1674 246 3079
Fragments arranged by size:
3079 1674 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6268
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 2084 3593 2084
Size: 1509 4759
MfeI C'AATT_G
Cuts at: 3320 3867 3320
Size: 547 5721
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 5382 5566 1305
Size: 893 3184 184 2007
Fragments arranged by size:
3184 2007 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 2772 10
Size: 54 1425 343 561 379 3506
Fragments arranged by size:
3506 1425 561 379 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2604 3215 4226 4585 4744 518
Size: 648 1108 330 611 1011 359 159 2042
Fragments arranged by size:
2042 1108 1011 648 611 359 330 159
MspA1I CmG'CkG
Cuts at: 1606 3197 3684 4361 5509 5754 1606
Size: 1591 487 677 1148 245 2120
Fragments arranged by size:
2120 1591 1148 677 487 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5716
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NsiI A_TGCA'T
Cuts at: 2228 2228
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2455 2936 3626 5171 1132
Size: 97 1226 481 690 1545 2229
Fragments arranged by size:
2229 1545 1226 690 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 2932 5167 2932
Size: 2235 4033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2495 2495
Size: 6268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 2244 3838 2244
Size: 1594 4674
PstI C_TGCA'G
Cuts at: 830 3620 830
Size: 2790 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
PvuII CAG'CTG
Cuts at: 3197 3197
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SapI GCTCTTCn'nnn_
Cuts at: 3429 3429
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2827 3616 4751 5432 5623 826
Size: 219 1782 789 1135 681 191 1471
Fragments arranged by size:
1782 1471 1135 789 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2942 4372 5273 5535 820
Size: 197 926 246 753 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 753 262 246 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 2455 3626 1132
Size: 1323 1171 3774
Fragments arranged by size:
3774 1323 1171
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2804 2850 3707 513
Size: 552 1739 46 857 3074
Fragments arranged by size:
3074 1739 857 552 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 4514 6226
Size: 274 80 33 51 1443 846 1691 1712
Cuts at: 6226 96
Size: 138
Fragments arranged by size:
1712 1691 1443 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2514 2514
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 3366 4397 3024
Size: 342 1031 4895
Fragments arranged by size:
4895 1031 342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI