Restriction map for
pFN21AE0011
(Circular) (Six-base) MAPSORT of: pFN21AE0011.seq Check: 3676 from: 1 to: 7144
pFN21AE0011 7144 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3762 4954 278
Size: 53 83 186 936 2226 1192 2468
Fragments arranged by size:
2468 2226 1192 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2390 4487 1937
Size: 453 2097 4594
Fragments arranged by size:
4594 2097 453
Acc65I G'GTAC_C
Cuts at: 4465 4465
Size: 7144
AclI AA'CG_TT
Cuts at: 1563 5271 5644 1563
Size: 3708 373 3063
Fragments arranged by size:
3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2652 3044 3683 3920 3977 4337 5207 6591
Size: 1020 392 639 237 57 360 870 1384
Cuts at: 6591 6859 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1020 870 639 392 360 268
237 57
AfeI AGC'GCT
Cuts at: 3288 3288
Size: 7144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6947
AflIII A'CryG_T
Cuts at: 1203 6043 7020 1203
Size: 4840 977 1327
Fragments arranged by size:
4840 1327 977
AgeI A'CCGG_T
Cuts at: 5975 5975
Size: 7144
AhdI GACnn_n'nnGTC
Cuts at: 5873 5873
Size: 7144
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7144
AlwNI CAG_nnn'CTG
Cuts at: 3661 3787 4887 6459 3661
Size: 126 1100 1572 4346
Fragments arranged by size:
4346 1572 1100 126
ApaLI G'TGCA_C
Cuts at: 3941 5201 6357 3941
Size: 1260 1156 4728
Fragments arranged by size:
4728 1260 1156
ApoI r'AATT_y
Cuts at: 1493 4453 4679 4831 6940 1493
Size: 2960 226 152 2109 1697
Fragments arranged by size:
2960 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5698 160
Size: 872 4666 1606
Fragments arranged by size:
4666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
AvaI C'yCGr_G
Cuts at: 1870 1943 3768 4469 1870
Size: 73 1825 701 4545
Fragments arranged by size:
4545 1825 701 73
BamHI G'GATC_C
Cuts at: 4474 4841 4474
Size: 367 6777
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 4465 5920 618
Size: 325 217 627 471 2207 1455 1842
Fragments arranged by size:
2207 1842 1455 627 471 325 217
BanII G_rGCy'C
Cuts at: 1391 2211 2232 3002 3463 3822 4463 1391
Size: 820 21 770 461 359 641 4072
Fragments arranged by size:
4072 820 770 641 461 359 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3482 3654 928
Size: 971 1583 172 4418
Fragments arranged by size:
4418 1583 971 172
BbvCI CC'TCA_GC
Cuts at: 2669 3290 4001 2669
Size: 621 711 5812
Fragments arranged by size:
5812 711 621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3260 3294 3721 3755 5335 5369 1903
Size: 34 1323 34 427 34 1580 34 3678
Fragments arranged by size:
3678 1580 1323 427 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3034 5036 6246 2628
Size: 406 2002 1210 3526
Fragments arranged by size:
3526 2002 1210 406
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5755 136
Size: 107 122 71 631 605 4083 1525
Fragments arranged by size:
4083 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7139 7139
Size: 7144
BlpI GC'TnA_GC
Cuts at: 2891 3905 4561 2891
Size: 1014 656 5474
Fragments arranged by size:
5474 1014 656
Bme1580I G_kGCm'C
Cuts at: 1790 3945 5205 6361 1790
Size: 2155 1260 1156 2573
Fragments arranged by size:
2573 2155 1260 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2892 4875 5833
Size: 416 53 83 399 24 1475 1983 958
Cuts at: 5833 442
Size: 1753
Fragments arranged by size:
1983 1753 1475 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4429 4429
Size: 7144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2483 2818 3435 3740 3893 3914 4399
Size: 818 426 335 617 305 153 21 485
Cuts at: 4399 5804 1239
Size: 1405 2579
Fragments arranged by size:
2579 1405 818 617 485 426 335 305
153 21
Bpu10I CC'TnA_GC
Cuts at: 2669 3290 4001 2669
Size: 621 711 5812
Fragments arranged by size:
5812 711 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3668 4364 5269 6134 6432 3437
Size: 231 696 905 865 298 4149
Fragments arranged by size:
4149 905 865 696 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2646 3411 4355 5807 882
Size: 617 1147 765 944 1452 2219
Fragments arranged by size:
2219 1452 1147 944 765 617
BsaAI yAC'GTr
Cuts at: 493 1204 4935 493
Size: 711 3731 2702
Fragments arranged by size:
3731 2702 711
BsaBI GATnn'nnATC
Cuts at: 7138 7138
Size: 7144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3759 4951 5333 5968
Size: 53 83 186 936 2226 1192 382 635
Cuts at: 5968 275
Size: 1451
Fragments arranged by size:
2226 1451 1192 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2727 2775 5577 5975 6249 6396 6807 2727
Size: 48 2802 398 274 147 411 3064
Fragments arranged by size:
3064 2802 411 398 274 147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 1178
Size: 30 165 30 634 30 6255
Fragments arranged by size:
6255 634 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 2794 3329 3851 3986 4418 4421 1186
Size: 1414 194 535 522 135 432 3 3909
Fragments arranged by size:
3909 1414 535 522 432 194 135 3
BseYI C'CCAG_C
Cuts at: 2663 2876 3858 4063 6347 6843 2663
Size: 213 982 205 2284 496 2964
Fragments arranged by size:
2964 2284 982 496 213 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2725 2837 3581 4117 1163
Size: 573 989 112 744 536 4190
Fragments arranged by size:
4190 989 744 573 536 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4594 5355 6383 1540
Size: 275 186 2593 761 1028 2301
Fragments arranged by size:
2593 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2232 2559 2897 3183 3463 3822 3911 3945
Size: 21 327 338 286 280 359 89 34
Cuts at: 3945 3954 5205 5290 6361 2211
Size: 9 1251 85 1071 2994
Fragments arranged by size:
2994 1251 1071 359 338 327 286 280
89 85 34 21 9
BsmI GAATG_Cn'
Cuts at: 2752 3493 4655 4748 2752
Size: 741 1162 93 5148
Fragments arranged by size:
5148 1162 741 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2075 2075
Size: 7144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2232 2559 2897 3002 3183 3463
Size: 399 421 21 327 338 105 181 280
Cuts at: 3463 3822 3911 3945 3954 4463 5205 5290 6361
Size: 359 89 34 9 509 742 85 1071
Cuts at: 6361 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 509 421 399 359 338
327 280 181 105 89 85 34 21
9
BspEI T'CCGG_A
Cuts at: 2775 6807 2775
Size: 4032 3112
BspHI T'CATG_A
Cuts at: 2145 3463 3507 5033 6763 2145
Size: 1318 44 1526 1730 2526
Fragments arranged by size:
2526 1730 1526 1318 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4499 844
Size: 3655 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2764 3392 5031 1394
Size: 429 941 628 1639 3507
Fragments arranged by size:
3507 1639 941 628 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2220 2917 3241 5639 5813 65
Size: 1183 972 697 324 2398 174 1396
Fragments arranged by size:
2398 1396 1183 972 697 324 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2769 3548 5788 5975 1700
Size: 110 959 779 2240 187 2869
Fragments arranged by size:
2869 2240 959 779 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7144
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7144
BssSI C'ACGA_G
Cuts at: 2321 5204 6216 2321
Size: 2883 1012 3249
Fragments arranged by size:
3249 2883 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2449 3991 794
Size: 1655 1542 3947
Fragments arranged by size:
3947 1655 1542
BstBI TT'CG_AA
Cuts at: 5948 5948
Size: 7144
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7144
BstXI CCAn_nnnn'nTGG
Cuts at: 3034 3217 3034
Size: 183 6961
BstYI r'GATC_y
Cuts at: 1972 3110 3790 4474 4841 5226 5243 6695 6781
Size: 1138 680 684 367 385 17 1452 86
Cuts at: 6781 6793 7139 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1138 684 680 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2394 2508 3543 4344 513
Size: 552 939 390 114 1035 801 3313
Fragments arranged by size:
3313 1035 939 801 552 390 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2908 487
Size: 683 56 1682 4723
Fragments arranged by size:
4723 1682 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 3037 3323 3810 4213 4660 5454 5474 2428
Size: 609 286 487 403 447 794 20 4098
Fragments arranged by size:
4098 794 609 487 447 403 286 20
ClaI AT'CG_AT
Cuts at: 1554 3741 1554
Size: 2187 4957
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7109
DraI TTT'AAA
Cuts at: 4448 4448
Size: 7144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6151 809
Size: 507 4835 1802
Fragments arranged by size:
4835 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2511 2605
Size: 54 1425 50 275 18 285 396 94
Cuts at: 2605 2842 2987 3546 3825 3843 4341 4591 5480
Size: 237 145 559 279 18 498 250 889
Cuts at: 5480 7109 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 559 498 396 285 279
275 250 237 145 94 54 50 43
18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4591 1537
Size: 275 2779 4090
Fragments arranged by size:
4090 2779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 2628 2670 2754 5074 1353
Size: 1130 145 42 84 2320 3423
Fragments arranged by size:
3423 2320 1130 145 84 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5731 6105 6251 387
Size: 1064 138 4142 374 146 1280
Fragments arranged by size:
4142 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2230 3461 3820 2209
Size: 21 1231 359 5533
Fragments arranged by size:
5533 1231 359 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2483 2652 2818 3044 3435 3683
Size: 393 425 426 169 166 226 391 248
Cuts at: 3683 3740 3893 3914 3920 3977 4337 4399 5207
Size: 57 153 21 6 57 360 62 808
Cuts at: 5207 5804 6591 6859 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 808 787 597 426 425 393 391
360 268 248 226 169 166 153 62
57 57 21 6
EcoO109I rG'GnC_Cy
Cuts at: 2095 2611 2828 3405 3889 2095
Size: 516 217 577 484 5350
Fragments arranged by size:
5350 577 516 484 217
EcoRI G'AATT_C
Cuts at: 4453 4831 4453
Size: 378 6766
FspI TGC'GCA
Cuts at: 5650 5650
Size: 7144
HaeII r_GCGC'y
Cuts at: 1678 3290 3350 6291 1678
Size: 1612 60 2941 2531
Fragments arranged by size:
2941 2531 1612 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2270 2289 2302
Size: 32 135 32 661 32 5 19 13
Cuts at: 2302 2321 2348 2380 2417 2449 3102 3134 3775
Size: 19 27 32 37 32 653 32 641
Cuts at: 3775 3807 4043 4075 5785 5817 5859 5891 1373
Size: 32 236 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 661 653 641 236 135 42
37 32 32 32 32 32 32 32
32 32 32 27 19 19 13 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2329 4488 4734 669
Size: 988 281 391 2159 246 3079
Fragments arranged by size:
3079 2159 988 391 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7144
HpaI GTT'AAC
Cuts at: 4734 4734
Size: 7144
KpnI G_GTAC'C
Cuts at: 4469 4469
Size: 7144
MfeI C'AATT_G
Cuts at: 4743 4743
Size: 7144
MluI A'CGCG_T
Cuts at: 7020 7020
Size: 7144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3609 4240 6258 6442 1305
Size: 2304 631 2018 184 2007
Fragments arranged by size:
2304 2018 2007 631 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 3827 4343 10
Size: 54 1425 343 285 1710 516 2811
Fragments arranged by size:
2811 1710 1425 516 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 3257 3446 3827 4428 5102 5461
Size: 648 994 1097 189 381 601 674 359
Cuts at: 5461 5620 518
Size: 159 2042
Fragments arranged by size:
2042 1097 994 674 648 601 381 359
189 159
MspA1I CmG'CkG
Cuts at: 1606 2496 3482 3525 3988 4017 4560 5237 6385
Size: 890 986 43 463 29 543 677 1148
Cuts at: 6385 6630 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 986 890 677 543 463 245
43 29
NaeI GCC'GGC
Cuts at: 3550 3550
Size: 7144
NcoI C'CATG_G
Cuts at: 513 1065 2004 2394 4344 513
Size: 552 939 390 1950 3313
Fragments arranged by size:
3313 1950 939 552 390
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7144
NgoMIV G'CCGG_C
Cuts at: 3548 3548
Size: 7144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7144
NotI GC'GGCC_GC
Cuts at: 4591 4591
Size: 7144
NspI r_CATG'y
Cuts at: 1132 1229 3256 4502 6047 1132
Size: 97 2027 1246 1545 2229
Fragments arranged by size:
2229 2027 1545 1246 97
PasI CC'CwG_GG
Cuts at: 1348 3567 1348
Size: 2219 4925
PciI A'CATG_T
Cuts at: 6043 6043
Size: 7144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3787 1108
Size: 2679 4465
PfoI T'CCnGG_A
Cuts at: 2166 2754 2166
Size: 588 6556
PmeI GTTT'AAAC
Cuts at: 4448 4448
Size: 7144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5213 5245 6726 6758 5213
Size: 32 1481 32 5599
Fragments arranged by size:
5599 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2611 2828 3405 2095
Size: 516 217 577 5834
Fragments arranged by size:
5834 577 516 217
PshAI GACnn'nnGTC
Cuts at: 1613 2366 1613
Size: 753 6391
PsiI TTA'TAA
Cuts at: 4714 4714
Size: 7144
PstI C_TGCA'G
Cuts at: 830 2423 2445 2501 3557 4234 4496 830
Size: 1593 22 56 1056 677 262 3478
Fragments arranged by size:
3478 1593 1056 677 262 56 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7144
PvuII CAG'CTG
Cuts at: 2496 3482 3525 2496
Size: 986 43 6115
Fragments arranged by size:
6115 986 43
SacI G_AGCT'C
Cuts at: 2211 2232 3463 3822 2211
Size: 21 1231 359 5533
Fragments arranged by size:
5533 1231 359 21
SalI G'TCGA_C
Cuts at: 1936 4486 1936
Size: 2550 4594
SapI GCTCTTCn'nnn_
Cuts at: 2483 2754 2483
Size: 271 6873
SbfI CC_TGCA'GG
Cuts at: 4496 4496
Size: 7144
ScaI AGT'ACT
Cuts at: 2285 5392 7104 2285
Size: 3107 1712 2325
Fragments arranged by size:
3107 2325 1712
SexAI A'CCwGG_T
Cuts at: 3936 4269 3936
Size: 333 6811
SfcI C'TryA_G
Cuts at: 826 1045 2419 2441 2497 2807 3553 4230 4492
Size: 219 1374 22 56 310 746 677 262
Cuts at: 4492 5627 6308 6499 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1374 1135 746 681 677 310 262
219 191 56 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7144
SmaI CCC'GGG
Cuts at: 1872 4471 1872
Size: 2599 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3452 3683 4379 5248 6149 6411
Size: 197 926 1509 231 696 869 901 262
Cuts at: 6411 820
Size: 1553
Fragments arranged by size:
1553 1509 926 901 869 696 262 231
197
SnaBI TAC'GTA
Cuts at: 493 4935 493
Size: 4442 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7144
SphI G_CATG'C
Cuts at: 1132 3256 4502 1132
Size: 2124 1246 3774
Fragments arranged by size:
3774 2124 1246
SspI AAT'ATT
Cuts at: 5 52 5068 5
Size: 47 5016 2081
Fragments arranged by size:
5016 2081 47
StuI AGG'CCT
Cuts at: 1517 3500 3932 1517
Size: 1983 432 4729
Fragments arranged by size:
4729 1983 432
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2394 3216 3495 4185 4344
Size: 552 939 114 276 822 279 690 159
Cuts at: 4344 4583 513
Size: 239 3074
Fragments arranged by size:
3074 939 822 690 552 279 276 239
159 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5171 5188 5341 5526 5971 325
Size: 1255 3591 17 153 185 445 1498
Fragments arranged by size:
3591 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3306 5390
Size: 274 80 33 51 1443 306 1023 2084
Cuts at: 5390 7102 96
Size: 1712 138
Fragments arranged by size:
2084 1712 1443 1023 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 3173 3205 3944 3976 7104 7136 2037
Size: 32 1104 32 739 32 3128 32 2045
Fragments arranged by size:
3128 2045 1104 739 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2962 1316
Size: 1646 5498
XbaI T'CTAG_A
Cuts at: 4480 4480
Size: 7144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3721 4267 3721
Size: 546 6598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7144
XmaI C'CCGG_G
Cuts at: 1870 4469 1870
Size: 2599 4545
XmnI GAAnn'nnTTC
Cuts at: 5273 5273
Size: 7144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3760 4952 276
Size: 53 83 186 936 2226 1192 2468
Fragments arranged by size:
2468 2226 1192 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI