Restriction map for
pFN21AE0008
(Circular) (Six-base) MAPSORT of: pFN21AE0008.seq Check: 3107 from: 1 to: 6796
pFN21AE0008 6796 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3539 3539
Size: 6796
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4606 278
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 4139 1937
Size: 1398 804 4594
Fragments arranged by size:
4594 1398 804
Acc65I G'GTAC_C
Cuts at: 4117 4117
Size: 6796
AclI AA'CG_TT
Cuts at: 1563 4923 5296 1563
Size: 3360 373 3063
Fragments arranged by size:
3360 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 4859 6243 6511 1632
Size: 450 2777 1384 268 1917
Fragments arranged by size:
2777 1917 1384 450 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6599
AflIII A'CryG_T
Cuts at: 1203 3310 5695 6672 1203
Size: 2107 2385 977 1327
Fragments arranged by size:
2385 2107 1327 977
AgeI A'CCGG_T
Cuts at: 5627 5627
Size: 6796
AhdI GACnn_n'nnGTC
Cuts at: 2801 5525 2801
Size: 2724 4072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6796
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6796
AlwNI CAG_nnn'CTG
Cuts at: 2750 4539 6111 2750
Size: 1789 1572 3435
Fragments arranged by size:
3435 1789 1572
ApaLI G'TGCA_C
Cuts at: 4853 6009 4853
Size: 1156 5640
ApoI r'AATT_y
Cuts at: 1493 2185 2430 2477 2784 2910 3144 3325 3646
Size: 692 245 47 307 126 234 181 321
Cuts at: 3646 4105 4331 4483 6592 1493
Size: 459 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 459 321 307 245 234
226 181 152 126 47
AseI AT'TA_AT
Cuts at: 160 1032 3681 5350 160
Size: 872 2649 1669 1606
Fragments arranged by size:
2649 1669 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
AvaI C'yCGr_G
Cuts at: 1870 1943 4121 1870
Size: 73 2178 4545
Fragments arranged by size:
4545 2178 73
BamHI G'GATC_C
Cuts at: 4126 4493 4126
Size: 367 6429
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4117 5572 618
Size: 325 217 627 2330 1455 1842
Fragments arranged by size:
2330 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3201 3544 4115 1391
Size: 1810 343 571 4072
Fragments arranged by size:
4072 1810 571 343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 3086 928
Size: 971 901 286 4638
Fragments arranged by size:
4638 971 901 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4987 5021 1903
Size: 34 3050 34 3678
Fragments arranged by size:
3678 3050 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4688 5898 4688
Size: 1210 5586
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6796
BfrBI ATG'CAT
Cuts at: 2092 2092
Size: 6796
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5407 136
Size: 107 122 71 631 605 3735 1525
Fragments arranged by size:
3735 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6791 6791
Size: 6796
BlpI GC'TnA_GC
Cuts at: 4213 4213
Size: 6796
Bme1580I G_kGCm'C
Cuts at: 1790 3519 4857 6013 1790
Size: 1729 1338 1156 2573
Fragments arranged by size:
2573 1729 1338 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6796
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3787 4527 5485
Size: 416 53 83 399 24 2370 740 958
Cuts at: 5485 442
Size: 1753
Fragments arranged by size:
2370 1753 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 5456 1239
Size: 1265 2952 2579
Fragments arranged by size:
2952 2579 1265
Bpu10I CC'TnA_GC
Cuts at: 2152 2152
Size: 6796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3172 4921 5786 6084 3172
Size: 1749 865 298 3884
Fragments arranged by size:
3884 1749 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3380 5459 882
Size: 617 1881 2079 2219
Fragments arranged by size:
2219 2079 1881 617
BsaAI yAC'GTr
Cuts at: 493 1204 2164 3495 4587 493
Size: 711 960 1331 1092 2702
Fragments arranged by size:
2702 1331 1092 960 711
BsaBI GATnn'nnATC
Cuts at: 6790 6790
Size: 6796
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4603 4985 5620 275
Size: 53 83 186 936 3070 382 635 1451
Fragments arranged by size:
3070 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5229 5627 5901 6048 6459 5229
Size: 398 274 147 411 5566
Fragments arranged by size:
5566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3782 3812 1178
Size: 30 165 30 2379 30 4162
Fragments arranged by size:
4162 2379 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6796
BseYI C'CCAG_C
Cuts at: 5999 6495 5999
Size: 496 6300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4246 5007 6035 1540
Size: 275 186 2245 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 4857 4942 6013 2417
Size: 2440 85 1071 3200
Fragments arranged by size:
3200 2440 1071 85
BsmI GAATG_Cn'
Cuts at: 2092 2849 3436 4307 4400 2092
Size: 757 587 871 93 4488
Fragments arranged by size:
4488 871 757 587 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 3201 3519 3544 4115 4857 4942
Size: 399 627 784 318 25 571 742 85
Cuts at: 4942 6013 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 784 742 627 571 399 318
85 25
BspEI T'CCGG_A
Cuts at: 6459 6459
Size: 6796
BspHI T'CATG_A
Cuts at: 4685 6415 4685
Size: 1730 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3539 4151 844
Size: 2695 612 3489
Fragments arranged by size:
3489 2695 612
BsrBI CCG'CTC
Cuts at: 1394 1823 4683 1394
Size: 429 2860 3507
Fragments arranged by size:
3507 2860 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 2502 5291 5465 65
Size: 1183 810 444 2789 174 1396
Fragments arranged by size:
2789 1396 1183 810 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5440 5627 1700
Size: 110 3630 187 2869
Fragments arranged by size:
3630 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3313 96
Size: 3217 3579
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6796
BssSI C'ACGA_G
Cuts at: 4856 5868 4856
Size: 1012 5784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6796
BstBI TT'CG_AA
Cuts at: 5600 5600
Size: 6796
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6796
BstYI r'GATC_y
Cuts at: 1972 3996 4126 4493 4878 4895 6347 6433 6445
Size: 2024 130 367 385 17 1452 86 12
Cuts at: 6445 6791 1972
Size: 346 1977
Fragments arranged by size:
2024 1977 1452 385 367 346 130 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2776 3186 3523 513
Size: 552 1711 410 337 3786
Fragments arranged by size:
3786 1711 552 410 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3918 487
Size: 683 56 2692 3365
Fragments arranged by size:
3365 2692 683 56
BtsI GCAGTG_nn'
Cuts at: 3293 3705 4312 5106 5126 3293
Size: 412 607 794 20 4963
Fragments arranged by size:
4963 794 607 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6761
DraI TTT'AAA
Cuts at: 2347 4100 2347
Size: 1753 5043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5803 809
Size: 507 4487 1802
Fragments arranged by size:
4487 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3909 4243 5132
Size: 54 1425 50 275 18 2079 334 889
Cuts at: 5132 6761 8
Size: 1629 43
Fragments arranged by size:
2079 1629 1425 889 334 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4243 1537
Size: 275 2431 4090
Fragments arranged by size:
4090 2431 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3206 4726 1353
Size: 1853 1520 3423
Fragments arranged by size:
3423 1853 1520
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5383 5757 5903 387
Size: 1064 138 3794 374 146 1280
Fragments arranged by size:
3794 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2504 4859 5456 6243 6511 1239
Size: 393 450 422 2355 597 787 268 1524
Fragments arranged by size:
2355 1524 787 597 450 422 393 268
EcoRI G'AATT_C
Cuts at: 4105 4483 4105
Size: 378 6418
FspI TGC'GCA
Cuts at: 5302 5302
Size: 6796
HaeII r_GCGC'y
Cuts at: 1678 5943 1678
Size: 4265 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2566 2598 2695
Size: 32 135 32 752 32 210 32 97
Cuts at: 2695 2727 2787 2819 2911 2943 5437 5469 5511
Size: 32 60 32 92 32 2494 32 42
Cuts at: 5511 5543 1373
Size: 32 2626
Fragments arranged by size:
2626 2494 752 210 135 97 92 60
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4140 4386 669
Size: 988 281 2202 246 3079
Fragments arranged by size:
3079 2202 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3450 748
Size: 2702 4094
HpaI GTT'AAC
Cuts at: 4386 4386
Size: 6796
KpnI G_GTAC'C
Cuts at: 4121 4121
Size: 6796
MfeI C'AATT_G
Cuts at: 4395 4395
Size: 6796
MluI A'CGCG_T
Cuts at: 6672 6672
Size: 6796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2473 3220 5910 6094 1305
Size: 1168 747 2690 184 2007
Fragments arranged by size:
2690 2007 1168 747 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3911 10
Size: 54 1425 343 2079 2895
Fragments arranged by size:
2895 2079 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 3522 4754 5113 5272 518
Size: 648 1350 1006 1232 359 159 2042
Fragments arranged by size:
2042 1350 1232 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4212 4889 6037 6282 1606
Size: 2606 677 1148 245 2120
Fragments arranged by size:
2606 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3186 3523 513
Size: 552 2121 337 3786
Fragments arranged by size:
3786 2121 552 337
NdeI CA'TA_TG
Cuts at: 387 3378 387
Size: 2991 3805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6796
NotI GC'GGCC_GC
Cuts at: 4243 4243
Size: 6796
NsiI A_TGCA'T
Cuts at: 2094 2094
Size: 6796
NspI r_CATG'y
Cuts at: 1132 1229 2092 3160 3314 3346 4154 5699 1132
Size: 97 863 1068 154 32 808 1545 2229
Fragments arranged by size:
2229 1545 1068 863 808 154 97 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6796
PciI A'CATG_T
Cuts at: 3310 5695 3310
Size: 2385 4411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2295 3140 3211 3918 1108
Size: 1187 845 71 707 3986
Fragments arranged by size:
3986 1187 845 707 71
PmeI GTTT'AAAC
Cuts at: 4100 4100
Size: 6796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4865 4897 6378 6410 4865
Size: 32 1481 32 5251
Fragments arranged by size:
5251 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6796
PsiI TTA'TAA
Cuts at: 4366 4366
Size: 6796
PstI C_TGCA'G
Cuts at: 830 3275 4148 830
Size: 2445 873 3478
Fragments arranged by size:
3478 2445 873
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6796
SalI G'TCGA_C
Cuts at: 1936 4138 1936
Size: 2202 4594
SapI GCTCTTCn'nnn_
Cuts at: 3206 3206
Size: 6796
SbfI CC_TGCA'GG
Cuts at: 4148 4148
Size: 6796
ScaI AGT'ACT
Cuts at: 5044 6756 5044
Size: 1712 5084
SfcI C'TryA_G
Cuts at: 826 1045 3271 4144 5279 5960 6151 826
Size: 219 2226 873 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 873 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6796
SmaI CCC'GGG
Cuts at: 1872 4123 1872
Size: 2251 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3151 4900 5801 6063 820
Size: 197 926 1208 1749 901 262 1553
Fragments arranged by size:
1749 1553 1208 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2164 3495 4587 493
Size: 1671 1331 1092 2702
Fragments arranged by size:
2702 1671 1331 1092
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6796
SphI G_CATG'C
Cuts at: 1132 3346 4154 1132
Size: 2214 808 3774
Fragments arranged by size:
3774 2214 808
SspI AAT'ATT
Cuts at: 5 52 2378 4720 5
Size: 47 2326 2342 2081
Fragments arranged by size:
2342 2326 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6796
StyI C'CwwG_G
Cuts at: 513 1065 3186 3523 3656 4235 513
Size: 552 2121 337 133 579 3074
Fragments arranged by size:
3074 2121 579 552 337 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3649 4823 4840 4993 5178 5623
Size: 1255 1956 113 1174 17 153 185 445
Cuts at: 5623 325
Size: 1498
Fragments arranged by size:
1956 1498 1255 1174 445 185 153 113
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3313 5042 6754
Size: 274 80 33 51 1443 1336 1729 1712
Cuts at: 6754 96
Size: 138
Fragments arranged by size:
1729 1712 1443 1336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3822 3854 3891 3923 6756 6788 3822
Size: 32 37 32 2833 32 3830
Fragments arranged by size:
3830 2833 37 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6796
XbaI T'CTAG_A
Cuts at: 4132 4132
Size: 6796
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6796
XmaI C'CCGG_G
Cuts at: 1870 4121 1870
Size: 2251 4545
XmnI GAAnn'nnTTC
Cuts at: 2597 2797 4925 2597
Size: 200 2128 4468
Fragments arranged by size:
4468 2128 200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4604 276
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI