Restriction map for
pFN21AE0006
(Circular) (Six-base) MAPSORT of: pFN21AE0006.seq Check: 5196 from: 1 to: 6268
pFN21AE0006 6268 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 3456 3611 1937
Size: 139 1380 155 4594
Fragments arranged by size:
4594 1380 155 139
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4331 5715 5983 1632
Size: 2699 1384 268 1917
Fragments arranged by size:
2699 1917 1384 268
AfeI AGC'GCT
Cuts at: 2221 2221
Size: 6268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 3107 1166
Size: 1941 4327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2598 2826 1184
Size: 1414 228 4626
Fragments arranged by size:
4626 1414 228
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 2823 2855 2714
Size: 32 77 32 6127
Fragments arranged by size:
6127 77 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2944 4011 5583 2944
Size: 1067 1572 3629
Fragments arranged by size:
3629 1572 1067
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 2111 2162 3146 3250 3577 3803 3955 6064
Size: 618 51 984 104 327 226 152 2109
Cuts at: 6064 1493
Size: 1697
Fragments arranged by size:
2109 1697 984 618 327 226 152 104
51
AseI AT'TA_AT
Cuts at: 160 1032 2469 4822 160
Size: 872 1437 2353 1606
Fragments arranged by size:
2353 1606 1437 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 2659 3593 1870
Size: 73 716 934 4545
Fragments arranged by size:
4545 934 716 73
BamHI G'GATC_C
Cuts at: 2838 3598 3965 2838
Size: 760 367 5141
Fragments arranged by size:
5141 760 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3589 5044 618
Size: 325 217 627 1802 1455 1842
Fragments arranged by size:
1842 1802 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3587 1391
Size: 2196 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3053 3087 4459 4493 1903
Size: 34 1116 34 1372 34 3678
Fragments arranged by size:
3678 1372 1116 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4879 136
Size: 107 122 71 631 605 3207 1525
Fragments arranged by size:
3207 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 4329 5485 1790
Size: 2539 1156 2573
Fragments arranged by size:
2573 2539 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3999 4957 442
Size: 416 53 83 399 24 2582 958 1753
Fragments arranged by size:
2582 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2238 2238
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 4928 1239
Size: 827 2862 2579
Fragments arranged by size:
2862 2579 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2996 4393 5258 5556 2702
Size: 294 1397 865 298 3414
Fragments arranged by size:
3414 1397 865 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 2671 4931 882
Size: 617 1090 82 2260 2219
Fragments arranged by size:
2260 2219 1090 617 82
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 3279 6262 3279
Size: 2983 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4701 5099 5373 5520 5931 4701
Size: 398 274 147 411 5038
Fragments arranged by size:
5038 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2991 3021 1178
Size: 30 165 30 1588 30 4425
Fragments arranged by size:
4425 1588 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2230 2999 1186
Size: 877 167 769 4455
Fragments arranged by size:
4455 877 769 167
BseYI C'CCAG_C
Cuts at: 2199 2571 5471 5967 2199
Size: 372 2900 496 2500
Fragments arranged by size:
2900 2500 496 372
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5695
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3587 4329 4414 5485 1391
Size: 399 1797 742 85 1071 2174
Fragments arranged by size:
2174 1797 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3623 844
Size: 2779 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2659 3189 4763 4937 65
Size: 1183 1411 530 1574 174 1396
Fragments arranged by size:
1574 1411 1396 1183 530 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3421 96
Size: 3325 2943
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2779 2838 3003 3598 3965 4350 4367 5819
Size: 807 59 165 595 367 385 17 1452
Cuts at: 5819 5905 5917 6263 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 807 595 385 367 346 165
86 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2395 513
Size: 552 939 186 205 4386
Fragments arranged by size:
4386 939 552 205 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 2710 3031 3784 4578 4598 2710
Size: 321 753 794 20 4380
Fragments arranged by size:
4380 794 753 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DraIII CAC_nnn'GTG
Cuts at: 3217 3217
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 3715 4604
Size: 54 1425 50 275 18 498 1387 889
Cuts at: 4604 6233 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1387 889 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2816 3033 4198 1353
Size: 1463 217 1165 3423
Fragments arranged by size:
3423 1463 1165 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2843 4855 5229 5375 387
Size: 1064 138 1254 2012 374 146 1280
Fragments arranged by size:
2012 1280 1254 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 4331 4928 5715 5983 1239
Size: 393 434 2265 597 787 268 1524
Fragments arranged by size:
2265 1524 787 597 434 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2195 2195
Size: 6268
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
EcoRV GAT'ATC
Cuts at: 2527 2527
Size: 6268
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2223 5415 1678
Size: 545 3192 2531
Fragments arranged by size:
3192 2531 545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2764 2796 2846 2878 3152
Size: 32 135 32 1192 32 50 32 274
Cuts at: 3152 3184 4909 4941 4983 5015 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 1192 274 135 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2634 3612 3858 669
Size: 988 281 696 978 246 3079
Fragments arranged by size:
3079 988 978 696 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6268
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2943 5382 5566 1305
Size: 1638 2439 184 2007
Fragments arranged by size:
2439 2007 1638 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 3948
Fragments arranged by size:
3948 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 3065 3107 4226 4585 4744 518
Size: 648 1122 777 42 1119 359 159 2042
Fragments arranged by size:
2042 1122 1119 777 648 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2203 2629 3684 4361 5509 5754 1606
Size: 597 426 1055 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 677 597 426 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2338 2483 2878 3626 5171 1132
Size: 97 1109 145 395 748 1545 2229
Fragments arranged by size:
2229 1545 1109 748 395 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2760 3319 1108
Size: 1652 559 4057
Fragments arranged by size:
4057 1652 559
PfoI T'CCnGG_A
Cuts at: 3255 3312 3255
Size: 57 6211
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2195
Size: 6268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 2495 3838 2495
Size: 1343 4925
PstI C_TGCA'G
Cuts at: 830 2940 3079 3274 3620 830
Size: 2110 139 195 346 3478
Fragments arranged by size:
3478 2110 346 195 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SanDI GG'GwC_CC
Cuts at: 2195 2195
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 2976 4516 6228 2976
Size: 1540 1712 3016
Fragments arranged by size:
3016 1712 1540
SfcI C'TryA_G
Cuts at: 826 1045 2073 2419 2512 2602 2936 3075 3270
Size: 219 1028 346 93 90 334 139 195
Cuts at: 3270 3616 4751 5432 5623 826
Size: 346 1135 681 191 1471
Fragments arranged by size:
1471 1135 1028 681 346 346 334 219
195 191 139 93 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3011 4372 5273 5535 820
Size: 197 926 774 294 1361 901 262 1553
Fragments arranged by size:
1553 1361 926 901 774 294 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 2483 3626 1132
Size: 1351 1143 3774
Fragments arranged by size:
3774 1351 1143
SspI AAT'ATT
Cuts at: 5 52 3503 4192 5
Size: 47 3451 689 2081
Fragments arranged by size:
3451 2081 689 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2004 3707 513
Size: 552 939 1703 3074
Fragments arranged by size:
3074 1703 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2924 2974 3421
Size: 274 80 33 51 1443 947 50 447
Cuts at: 3421 4514 6226 96
Size: 1093 1712 138
Fragments arranged by size:
1712 1443 1093 947 447 274 138 80
51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 2895 4397 2895
Size: 1502 4766
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI