Restriction map for
pFN21AE0003
(Circular) (Six-base) MAPSORT of: pFN21AE0003.seq Check: 1884 from: 1 to: 6247
pFN21AE0003 6247 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2636 4057 278
Size: 53 83 186 936 1100 1421 2468
Fragments arranged by size:
2468 1421 1100 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3435 3590 1937
Size: 1498 155 4594
Fragments arranged by size:
4594 1498 155
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 3546 4374 4747 1563
Size: 1983 828 373 3063
Fragments arranged by size:
3063 1983 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 2516 3354 4310 5694 5962 1632
Size: 694 190 838 956 1384 268 1917
Fragments arranged by size:
1917 1384 956 838 694 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 3138 5146 6123 1203
Size: 1935 2008 977 1327
Fragments arranged by size:
2008 1935 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 3506 3990 5562 3506
Size: 484 1572 4191
Fragments arranged by size:
4191 1572 484
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2255 2780 3556 3782 3934 6043 1493
Size: 762 525 776 226 152 2109 1697
Fragments arranged by size:
2109 1697 776 762 525 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3010 3351 3572 1870
Size: 73 1067 341 221 4545
Fragments arranged by size:
4545 1067 341 221 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3568 5023 618
Size: 325 217 627 1781 1455 1842
Fragments arranged by size:
1842 1781 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3473 3566 1391
Size: 2082 93 4072
Fragments arranged by size:
4072 2082 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3384 3435 928
Size: 971 1485 51 3740
Fragments arranged by size:
3740 1485 971 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 3508 1548
Size: 1960 4287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 4308 5464 1790
Size: 2518 1156 2573
Fragments arranged by size:
2573 2518 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2563 2697
Size: 416 53 83 399 24 682 464 134
Cuts at: 2697 2785 3978 4936 442
Size: 88 1193 958 1753
Fragments arranged by size:
1753 1193 958 682 464 416 399 134
88 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 4907 1239
Size: 976 2692 2579
Fragments arranged by size:
2692 2579 976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3487 4372 5237 5535 3487
Size: 885 865 298 4199
Fragments arranged by size:
4199 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 4910 882
Size: 617 550 2861 2219
Fragments arranged by size:
2861 2219 617 550
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 2811 6241 2811
Size: 3430 2817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2633 4054 4436 5071
Size: 53 83 186 936 1100 1421 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1451 1421 1100 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6022
Fragments arranged by size:
6022 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 1186
Size: 1013 5234
BseYI C'CCAG_C
Cuts at: 2673 5450 5946 2673
Size: 2777 496 2974
Fragments arranged by size:
2974 2777 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3473 4308 4393 5464 3473
Size: 835 85 1071 4256
Fragments arranged by size:
4256 1071 835 85
BsmI GAATG_Cn'
Cuts at: 2723 3758 3851 2723
Size: 1035 93 5119
Fragments arranged by size:
5119 1035 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3473 3566 4308 4393 5464 1391
Size: 399 1683 93 742 85 1071 2174
Fragments arranged by size:
2174 1683 1071 742 399 93 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 2460 2986 4136 5866 2460
Size: 526 1150 1730 2841
Fragments arranged by size:
2841 1730 1150 526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3144 3602 844
Size: 2300 458 3489
Fragments arranged by size:
3489 2300 458
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2729 794
Size: 1935 4312
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2947 3577 3944 4329 4346 5798 5884 5896
Size: 975 630 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 630 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 3763 4557 4577 3763
Size: 794 20 5433
Fragments arranged by size:
5433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3051 3551 3051
Size: 500 5747
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 4177 1353
Size: 1013 1811 3423
Fragments arranged by size:
3423 1811 1013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3471 3471
Size: 6247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2326 2516 3354 4310 4907 5694
Size: 393 583 111 190 838 956 597 787
Cuts at: 5694 5962 1239
Size: 268 1524
Fragments arranged by size:
1524 956 838 787 597 583 393 268
190 111
EcoO109I rG'GnC_Cy
Cuts at: 2101 2481 2101
Size: 380 5867
EcoRI G'AATT_C
Cuts at: 2780 3556 3934 2780
Size: 776 378 5093
Fragments arranged by size:
5093 776 378
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2406 2438 2932
Size: 32 135 32 734 32 68 32 494
Cuts at: 2932 2964 3079 3111 4888 4920 4962 4994 1373
Size: 32 115 32 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 734 494 135 115 68 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6247
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2125 5361 5545 1305
Size: 820 3236 184 2007
Fragments arranged by size:
3236 2007 820 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2522 2991 3302 4205 4564 4723
Size: 648 888 468 469 311 903 359 159
Cuts at: 4723 518
Size: 2042
Fragments arranged by size:
2042 903 888 648 469 468 359 311
159
MspA1I CmG'CkG
Cuts at: 1606 2673 3663 4340 5488 5733 1606
Size: 1067 990 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 990 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 3124 387
Size: 2737 3510
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 2842 3142 3605 5150 1132
Size: 97 1613 300 463 1545 2229
Fragments arranged by size:
2229 1613 1545 463 300 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 3138 5146 3138
Size: 2008 4239
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2481 2101
Size: 380 5867
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 2979 3817 2979
Size: 838 5409
PstI C_TGCA'G
Cuts at: 830 2476 3599 830
Size: 1646 1123 3478
Fragments arranged by size:
3478 1646 1123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2673 2673
Size: 6247
SacI G_AGCT'C
Cuts at: 3473 3473
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 2472 3595 4730 5411 5602 826
Size: 219 1427 1123 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 1123 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3012 3574 1872
Size: 1140 562 4545
Fragments arranged by size:
4545 1140 562
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3351 3466 4351 5252 5514 820
Size: 197 926 1408 115 885 901 262 1553
Fragments arranged by size:
1553 1408 926 901 885 262 197 115
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 3491 152
Size: 3339 2908
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 2091 2973 4171 5
Size: 47 2039 882 1198 2081
Fragments arranged by size:
2081 2039 1198 882 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 3686 513
Size: 552 939 1682 3074
Fragments arranged by size:
3074 1682 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3377 4274 4291 4444 4629 5074 325
Size: 1255 1797 897 17 153 185 445 1498
Fragments arranged by size:
1797 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 3533 4493
Size: 274 80 33 51 1443 667 889 960
Cuts at: 4493 6205 96
Size: 1712 138
Fragments arranged by size:
1712 1443 960 889 667 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6207 6239 6207
Size: 32 6215
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 1316
Size: 1182 5065
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 3351 1943
Size: 1408 4839
XmaI C'CCGG_G
Cuts at: 1870 3010 3572 1870
Size: 1140 562 4545
Fragments arranged by size:
4545 1140 562
XmnI GAAnn'nnTTC
Cuts at: 2870 4376 2870
Size: 1506 4741
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2634 4055 276
Size: 53 83 186 936 1100 1421 2468
Fragments arranged by size:
2468 1421 1100 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI