Restriction map for
pFN21AB9999
(Circular) (Six-base) MAPSORT of: pFN21AB9999.seq Check: 2289 from: 1 to: 8701
pFN21AB9999 8701 bp
With 182 enzymes: SgfI *
November 11, 2011 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3415 5602 3415
Size: 2187 6514
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6511 278
Size: 53 83 186 936 4975 2468
Fragments arranged by size:
4975 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6044 1937
Size: 4107 4594
Acc65I G'GTAC_C
Cuts at: 6022 6022
Size: 8701
AclI AA'CG_TT
Cuts at: 1563 5274 6828 7201 1563
Size: 3711 1554 373 3063
Fragments arranged by size:
3711 3063 1554 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2777 2906 3262 3541 3764 4172 5266
Size: 614 531 129 356 279 223 408 1094
Cuts at: 5266 5924 6764 8148 8416 1632
Size: 658 840 1384 268 1917
Fragments arranged by size:
1917 1384 1094 840 658 614 531 408
356 279 268 223 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8504
AflIII A'CryG_T
Cuts at: 1203 3012 7600 8577 1203
Size: 1809 4588 977 1327
Fragments arranged by size:
4588 1809 1327 977
AgeI A'CCGG_T
Cuts at: 7532 7532
Size: 8701
AhdI GACnn_n'nnGTC
Cuts at: 2249 7430 2249
Size: 5181 3520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 1184
Size: 1170 7531
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 8669
AlwNI CAG_nnn'CTG
Cuts at: 2500 3742 4408 5038 5251 5289 5708 5971 6444
Size: 1242 666 630 213 38 419 263 473
Cuts at: 6444 8016 2500
Size: 1572 3185
Fragments arranged by size:
3185 1572 1242 666 630 473 419 263
213 38
ApaI G_GGCC'C
Cuts at: 4702 4892 5072 4702
Size: 190 180 8331
Fragments arranged by size:
8331 190 180
ApaLI G'TGCA_C
Cuts at: 3016 6758 7914 3016
Size: 3742 1156 3803
Fragments arranged by size:
3803 3742 1156
ApoI r'AATT_y
Cuts at: 1493 6010 6236 6388 8497 1493
Size: 4517 226 152 2109 1697
Fragments arranged by size:
4517 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7255 160
Size: 872 6223 1606
Fragments arranged by size:
6223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 3531 4088 4523 4929 5153 5798
Size: 73 303 1285 557 435 406 224 645
Cuts at: 5798 6026 1870
Size: 228 4545
Fragments arranged by size:
4545 1285 645 557 435 406 303 228
224 73
BamHI G'GATC_C
Cuts at: 2017 2705 6031 6398 2017
Size: 688 3326 367 4320
Fragments arranged by size:
4320 3326 688 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2677 3074 3090 3595
Size: 325 217 627 273 617 397 16 505
Cuts at: 3595 5016 5711 6022 7477 618
Size: 1421 695 311 1455 1842
Fragments arranged by size:
1842 1455 1421 695 627 617 505 397
325 311 273 217 16
BanII G_rGCy'C
Cuts at: 1391 2655 3203 4702 4892 4955 5072 5495 5729
Size: 1264 548 1499 190 63 117 423 234
Cuts at: 5729 5797 6020 1391
Size: 68 223 4072
Fragments arranged by size:
4072 1499 1264 548 423 234 223 190
117 68 63
BbeI G_GCGC'C
Cuts at: 2064 5715 2064
Size: 3651 5050
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2844 5117 928
Size: 971 423 522 2273 4512
Fragments arranged by size:
4512 2273 971 522 423
BbvCI CC'TCA_GC
Cuts at: 3347 3575 4821 5000 3347
Size: 228 1246 179 7048
Fragments arranged by size:
7048 1246 228 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6892 6926 1903
Size: 34 4955 34 3678
Fragments arranged by size:
4955 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 6593 7803 4424
Size: 2169 1210 5322
Fragments arranged by size:
5322 2169 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2931 3536 3980
Size: 107 122 71 631 605 1259 605 444
Cuts at: 3980 4364 7312 136
Size: 384 2948 1525
Fragments arranged by size:
2948 1525 1259 631 605 605 444 384
122 107 71
BglII A'GATC_T
Cuts at: 3963 8696 3963
Size: 4733 3968
BlpI GC'TnA_GC
Cuts at: 3024 4562 6118 3024
Size: 1538 1556 5607
Fragments arranged by size:
5607 1556 1538
Bme1580I G_kGCm'C
Cuts at: 1790 2682 3020 3077 4060 4663 4702 4892 5072
Size: 892 338 57 983 603 39 190 180
Cuts at: 5072 5374 6762 7918 1790
Size: 302 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 983 892 603 338 302
190 180 57 39
BmgBI CAC'GTC
Cuts at: 1318 2989 4258 1318
Size: 1671 1269 5761
Fragments arranged by size:
5761 1671 1269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 5472 6432
Size: 416 53 83 399 24 1083 2972 960
Cuts at: 6432 7390 442
Size: 958 1753
Fragments arranged by size:
2972 1753 1083 960 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2415 3778 2415
Size: 1363 7338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2198 3233 3419 3773 4562 4571 5090
Size: 822 137 1035 186 354 789 9 519
Cuts at: 5090 5282 5371 7361 1239
Size: 192 89 1990 2579
Fragments arranged by size:
2579 1990 1035 822 789 519 354 192
186 137 89 9
Bpu10I CC'TnA_GC
Cuts at: 2139 3105 3197 3327 3347 3575 4821 5000 5658
Size: 966 92 130 20 228 1246 179 658
Cuts at: 5658 2139
Size: 5182
Fragments arranged by size:
5182 1246 966 658 228 179 130 92
20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2816 3809 6826 7691 7989 2675
Size: 141 993 3017 865 298 3387
Fragments arranged by size:
3387 3017 993 865 298 141
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2386 2572 4085 7364 882
Size: 617 887 186 1513 3279 2219
Fragments arranged by size:
3279 2219 1513 887 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 3013 3070 6492 493
Size: 711 1809 57 3422 2702
Fragments arranged by size:
3422 2702 1809 711 57
BsaBI GATnn'nnATC
Cuts at: 8695 8695
Size: 8701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 5684 5712 6508
Size: 53 83 186 936 528 3623 28 796
Cuts at: 6508 6890 7525 275
Size: 382 635 1451
Fragments arranged by size:
3623 1451 936 796 635 528 382 186
83 53 28
BsaWI w'CCGG_w
Cuts at: 3726 7134 7532 7806 7953 8364 3726
Size: 3408 398 274 147 411 4063
Fragments arranged by size:
4063 3408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 2999 3029 3248
Size: 30 165 30 1140 30 426 30 219
Cuts at: 3248 3278 3491 3521 4022 4052 5120 5150 1178
Size: 30 213 30 501 30 1068 30 4729
Fragments arranged by size:
4729 1140 1068 501 426 219 213 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 3422 3794 3812 4226 4241 4250 4253
Size: 1575 661 372 18 414 15 9 3
Cuts at: 4253 4256 4352 4355 1186
Size: 3 96 3 5532
Fragments arranged by size:
5532 1575 661 414 372 96 18 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2025 2667 2929 3853 3984 3989 4301 4312 4345
Size: 642 262 924 131 5 312 11 33
Cuts at: 4345 4871 5212 5621 5665 5729 5930 7904 8400
Size: 526 341 409 44 64 201 1974 496
Cuts at: 8400 2025
Size: 2326
Fragments arranged by size:
2326 1974 924 642 526 496 409 341
312 262 201 131 64 44 33 11
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 2951 3377 3662 4179 4604 4688
Size: 573 614 601 426 285 517 425 84
Cuts at: 4688 4748 5381 1163
Size: 60 633 4483
Fragments arranged by size:
4483 633 614 601 573 517 426 425
285 84 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 6151 6912 7940 1540
Size: 275 186 76 4074 761 1028 2301
Fragments arranged by size:
4074 2301 1028 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2427 2655 3012 3020 4955 5028 5328 5797 6762
Size: 228 357 8 1935 73 300 469 965
Cuts at: 6762 6847 7918 2427
Size: 85 1071 3210
Fragments arranged by size:
3210 1935 1071 965 469 357 300 228
85 73 8
BsmI GAATG_Cn'
Cuts at: 4534 6212 6305 4534
Size: 1678 93 6930
Fragments arranged by size:
6930 1678 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2141 2979 4422 5528 2141
Size: 838 1443 1106 5314
Fragments arranged by size:
5314 1443 1106 838
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2655 2682 3012 3020 3077 3203
Size: 399 637 228 27 330 8 57 126
Cuts at: 3203 4060 4663 4702 4892 4955 5028 5072 5328
Size: 857 603 39 190 63 73 44 256
Cuts at: 5328 5374 5495 5729 5797 6020 6762 6847 7918
Size: 46 121 234 68 223 742 85 1071
Cuts at: 7918 1391
Size: 2174
Fragments arranged by size:
2174 1071 857 742 637 603 399 330
256 234 228 223 190 126 121 85
73 68 63 57 46 44 39 27
8
BspEI T'CCGG_A
Cuts at: 8364 8364
Size: 8701
BspHI T'CATG_A
Cuts at: 6590 8320 6590
Size: 1730 6971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 2404 2823 3415 3858 3878 4800 5297
Size: 1529 31 419 592 443 20 922 497
Cuts at: 5297 5602 5824 6056 844
Size: 305 222 232 3489
Fragments arranged by size:
3489 1529 922 592 497 443 419 305
232 222 31 20
BsrBI CCG'CTC
Cuts at: 1394 1823 3109 4144 4720 6588 1394
Size: 429 1286 1035 576 1868 3507
Fragments arranged by size:
3507 1868 1286 1035 576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2391 2579 2802 7196 7370 65
Size: 1183 879 264 188 223 4394 174 1396
Fragments arranged by size:
4394 1396 1183 879 264 223 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5381 5603 7345 7532 1700
Size: 110 3571 222 1742 187 2869
Fragments arranged by size:
3571 2869 1742 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8701
BssHII G'CGCG_C
Cuts at: 1925 4631 4690 1925
Size: 2706 59 5936
Fragments arranged by size:
5936 2706 59
BssSI C'ACGA_G
Cuts at: 3806 6761 7773 3806
Size: 2955 1012 4734
Fragments arranged by size:
4734 2955 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2677 3799 4408 5023 794
Size: 1883 1122 609 615 4472
Fragments arranged by size:
4472 1883 1122 615 609
BstBI TT'CG_AA
Cuts at: 7505 7505
Size: 8701
BstEII G'GTnAC_C
Cuts at: 1184 4924 5191 1184
Size: 3740 267 4694
Fragments arranged by size:
4694 3740 267
BstXI CCAn_nnnn'nTGG
Cuts at: 2809 2856 4909 5789 2809
Size: 47 2053 880 5721
Fragments arranged by size:
5721 2053 880 47
BstYI r'GATC_y
Cuts at: 1972 2017 2589 2705 3483 3963 4479 5472 6031
Size: 45 572 116 778 480 516 993 559
Cuts at: 6031 6398 6783 6800 8252 8338 8350 8696 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 993 778 572 559 516 480
385 367 346 116 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2163 2849 3941 4884 5808 513
Size: 552 939 159 686 1092 943 924 3406
Fragments arranged by size:
3406 1092 943 939 924 686 552 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3297 487
Size: 683 56 2071 5891
Fragments arranged by size:
5891 2071 683 56
BtsI GCAGTG_nn'
Cuts at: 3018 3553 3699 4815 6217 7011 7031 3018
Size: 535 146 1116 1402 794 20 4688
Fragments arranged by size:
4688 1402 1116 794 535 146 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8666
DraI TTT'AAA
Cuts at: 6005 6005
Size: 8701
DraIII CAC_nnn'GTG
Cuts at: 2974 3640 3679 5023 2974
Size: 666 39 1344 6652
Fragments arranged by size:
6652 1344 666 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7708 809
Size: 507 6392 1802
Fragments arranged by size:
6392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2763 2900
Size: 54 1425 50 275 18 330 603 137
Cuts at: 2900 3117 3429 4027 4738 6148 7037 8666 8
Size: 217 312 598 711 1410 889 1629 43
Fragments arranged by size:
1629 1425 1410 889 711 603 598 330
312 275 217 137 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6148 1537
Size: 275 4336 4090
Fragments arranged by size:
4336 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 4203 5012 5285 5633 5850 6631 1353
Size: 1307 1543 809 273 348 217 781 3423
Fragments arranged by size:
3423 1543 1307 809 781 348 273 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2810 2912 2984 7288 7662 7808
Size: 1064 138 1221 102 72 4304 374 146
Cuts at: 7808 387
Size: 1280
Fragments arranged by size:
4304 1280 1221 1064 374 146 138 102
72
EcoICRI GAG'CTC
Cuts at: 2653 4953 5795 2653
Size: 2300 842 5559
Fragments arranged by size:
5559 2300 842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2198 2246 2777 2906 3233 3262
Size: 393 429 137 48 531 129 327 29
Cuts at: 3262 3419 3541 3764 3773 4172 4562 4571 5090
Size: 157 122 223 9 399 390 9 519
Cuts at: 5090 5266 5282 5371 5924 6764 7361 8148 8416
Size: 176 16 89 553 840 597 787 268
Cuts at: 8416 1239
Size: 1524
Fragments arranged by size:
1524 840 787 597 553 531 519 429
399 393 390 327 268 223 176 157
137 129 122 89 48 29 16 9
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2561 2597 3874 4499 5186 5363 2369
Size: 192 36 1277 625 687 177 5707
Fragments arranged by size:
5707 1277 687 625 192 177 36
EcoO109I rG'GnC_Cy
Cuts at: 2497 2542 2602 4612 4698 4699 5149 5179 5286
Size: 45 60 2010 86 1 450 30 107
Cuts at: 5286 5662 5802 2497
Size: 376 140 5396
Fragments arranged by size:
5396 2010 450 376 140 107 86 60
45 30 1
EcoRI G'AATT_C
Cuts at: 6010 6388 6010
Size: 378 8323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5326 5542 5878 5928 5326
Size: 216 336 50 8099
Fragments arranged by size:
8099 336 216 50
FspI TGC'GCA
Cuts at: 3705 4586 7207 3705
Size: 881 2621 5199
Fragments arranged by size:
5199 2621 881
HaeII r_GCGC'y
Cuts at: 1678 2064 2831 3715 4313 4669 5443 5715 7848
Size: 386 767 884 598 356 774 272 2133
Cuts at: 7848 1678
Size: 2531
Fragments arranged by size:
2531 2133 884 774 767 598 386 356
272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2235 2267 2385
Size: 32 135 32 430 32 201 32 118
Cuts at: 2385 2417 2543 2575 2976 3008 3248 3280 3525
Size: 32 126 32 401 32 240 32 245
Cuts at: 3525 3557 5120 5152 5967 5999 7342 7374 7416
Size: 32 1563 32 815 32 1343 32 42
Cuts at: 7416 7448 1373
Size: 32 2626
Fragments arranged by size:
2626 1563 1343 815 430 401 245 240
201 135 126 118 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3274 6045 6291 669
Size: 988 281 1336 2771 246 3079
Fragments arranged by size:
3079 2771 1336 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8701
HpaI GTT'AAC
Cuts at: 6291 6291
Size: 8701
KasI G'GCGC_C
Cuts at: 2060 5711 2060
Size: 3651 5050
KpnI G_GTAC'C
Cuts at: 6026 6026
Size: 8701
MfeI C'AATT_G
Cuts at: 6300 6300
Size: 8701
MluI A'CGCG_T
Cuts at: 8577 8577
Size: 8701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5430 7815 7999 1305
Size: 4125 2385 184 2007
Fragments arranged by size:
4125 2385 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2902 3431 4029 10
Size: 54 1425 343 1070 529 598 4682
Fragments arranged by size:
4682 1425 1070 598 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6659 7018 7177 518
Size: 648 5493 359 159 2042
Fragments arranged by size:
5493 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2965 3115 4282 4558 4905 5167 5579
Size: 693 666 150 1167 276 347 262 412
Cuts at: 5579 6117 6794 7942 8187 1606
Size: 538 677 1148 245 2120
Fragments arranged by size:
2120 1167 1148 693 677 666 538 412
347 276 262 245 150
NarI GG'CG_CC
Cuts at: 2061 5712 2061
Size: 3651 5050
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7210
Fragments arranged by size:
7210 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8701
NotI GC'GGCC_GC
Cuts at: 6148 6148
Size: 8701
NruI TCG'CGA
Cuts at: 2103 2103
Size: 8701
NspI r_CATG'y
Cuts at: 1132 1229 3325 4882 6059 7604 1132
Size: 97 2096 1557 1177 1545 2229
Fragments arranged by size:
2229 2096 1557 1545 1177 97
PasI CC'CwG_GG
Cuts at: 1348 3610 5428 1348
Size: 2262 1818 4621
Fragments arranged by size:
4621 2262 1818
PciI A'CATG_T
Cuts at: 7600 7600
Size: 8701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4681 5708 1108
Size: 3573 1027 4101
Fragments arranged by size:
4101 3573 1027
PfoI T'CCnGG_A
Cuts at: 4272 4539 4272
Size: 267 8434
PmeI GTTT'AAAC
Cuts at: 6005 6005
Size: 8701
PmlI CAC'GTG
Cuts at: 3013 3013
Size: 8701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 6770 6802 8283 8315 2705
Size: 32 4033 32 1481 32 3091
Fragments arranged by size:
4033 3091 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 4612 5149 5179 5286 5662 5802 2602
Size: 2010 537 30 107 376 140 5501
Fragments arranged by size:
5501 2010 537 376 140 107 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8701
PsiI TTA'TAA
Cuts at: 6271 6271
Size: 8701
PspOMI G'GGCC_C
Cuts at: 4698 4888 5068 4698
Size: 190 180 8331
Fragments arranged by size:
8331 190 180
PstI C_TGCA'G
Cuts at: 830 2343 2496 3216 3738 3838 4065 4299 4449
Size: 1513 153 720 522 100 227 234 150
Cuts at: 4449 5343 5599 5760 5821 6053 830
Size: 894 256 161 61 232 3478
Fragments arranged by size:
3478 1513 894 720 522 256 234 232
227 161 153 150 100 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8701
PvuII CAG'CTG
Cuts at: 2299 2965 3115 4282 4905 5167 2299
Size: 666 150 1167 623 262 5833
Fragments arranged by size:
5833 1167 666 623 262 150
SacI G_AGCT'C
Cuts at: 2655 4955 5797 2655
Size: 2300 842 5559
Fragments arranged by size:
5559 2300 842
SalI G'TCGA_C
Cuts at: 1936 6043 1936
Size: 4107 4594
SanDI GG'GwC_CC
Cuts at: 5802 5802
Size: 8701
SapI GCTCTTCn'nnn_
Cuts at: 2660 5850 2660
Size: 3190 5511
SbfI CC_TGCA'GG
Cuts at: 3216 5821 6053 3216
Size: 2605 232 5864
Fragments arranged by size:
5864 2605 232
ScaI AGT'ACT
Cuts at: 6949 8661 6949
Size: 1712 6989
SexAI A'CCwGG_T
Cuts at: 2970 3636 4494 4920 2970
Size: 666 858 426 6751
Fragments arranged by size:
6751 858 666 426
SfcI C'TryA_G
Cuts at: 826 1045 2278 2339 2492 3212 3734 3834 4061
Size: 219 1233 61 153 720 522 100 227
Cuts at: 4061 4295 4445 5339 5360 5595 5756 5817 6049
Size: 234 150 894 21 235 161 61 232
Cuts at: 6049 7184 7865 8056 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 894 720 681 522 235
234 232 227 219 191 161 153 150
100 61 61 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3980 4364 3980
Size: 384 8317
SfoI GGC'GCC
Cuts at: 2062 5713 2062
Size: 3651 5050
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
SgrAI Cr'CCGG_yG
Cuts at: 1700 5603 1700
Size: 3903 4798
SmaI CCC'GGG
Cuts at: 1872 4931 5800 6028 1872
Size: 3059 869 228 4545
Fragments arranged by size:
4545 3059 869 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 2831 3824 6805 7706 7968
Size: 197 926 747 141 993 2981 901 262
Cuts at: 7968 820
Size: 1553
Fragments arranged by size:
2981 1553 993 926 901 747 262 197
141
SnaBI TAC'GTA
Cuts at: 493 6492 493
Size: 5999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8701
SphI G_CATG'C
Cuts at: 1132 6059 1132
Size: 4927 3774
SspI AAT'ATT
Cuts at: 5 52 6625 5
Size: 47 6573 2081
Fragments arranged by size:
6573 2081 47
StuI AGG'CCT
Cuts at: 1517 3156 3413 3719 5093 1517
Size: 1639 257 306 1374 5125
Fragments arranged by size:
5125 1639 1374 306 257
StyI C'CwwG_G
Cuts at: 513 1065 2004 2955 3151 3267 5229 5310 5466
Size: 552 939 951 196 116 1962 81 156
Cuts at: 5466 6140 513
Size: 674 3074
Fragments arranged by size:
3074 1962 951 939 674 552 196 156
116 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 4304 4673 5321 5501 6728 6745
Size: 1255 2265 459 369 648 180 1227 17
Cuts at: 6745 6898 7083 7528 325
Size: 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 1227 648 459 445 369
185 180 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4935 6947 8659
Size: 274 80 33 51 1443 2958 2012 1712
Cuts at: 8659 96
Size: 138
Fragments arranged by size:
2958 2012 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 3491 3523 3926 3958 4022 4054 5141
Size: 32 460 32 403 32 64 32 1087
Cuts at: 5141 5173 5683 5715 8661 8693 2999
Size: 32 510 32 2946 32 3007
Fragments arranged by size:
3007 2946 1087 510 460 403 64 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5116 1316
Size: 3800 4901
XbaI T'CTAG_A
Cuts at: 6037 6037
Size: 8701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3611 4366 5425 3611
Size: 755 1059 6887
Fragments arranged by size:
6887 1059 755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8701
XmaI C'CCGG_G
Cuts at: 1870 4929 5798 6026 1870
Size: 3059 869 228 4545
Fragments arranged by size:
4545 3059 869 228
XmnI GAAnn'nnTTC
Cuts at: 2717 6830 2717
Size: 4113 4588
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6509 276
Size: 53 83 186 936 4975 2468
Fragments arranged by size:
4975 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI
PsrI RsrII SacII SrfI SwaI