Restriction map for
pFN21AB9997
(Circular) (Six-base) MAPSORT of: pFN21AB9997.seq Check: 3336 from: 1 to: 8224
pFN21AB9997 8224 bp
With 182 enzymes: SgfI *
March 28, 2012 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5409 5409
Size: 8224
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6034 278
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 5567 1937
Size: 1725 1905 4594
Fragments arranged by size:
4594 1905 1725
Acc65I G'GTAC_C
Cuts at: 4516 5545 4516
Size: 1029 7195
AclI AA'CG_TT
Cuts at: 1563 6351 6724 1563
Size: 4788 373 3063
Fragments arranged by size:
4788 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2487 4076 4772 4976 6287 7671 7939
Size: 846 9 1589 696 204 1311 1384 268
Cuts at: 7939 1632
Size: 1917
Fragments arranged by size:
1917 1589 1384 1311 846 696 268 204
9
AfeI AGC'GCT
Cuts at: 2020 4061 2020
Size: 2041 6183
AflII C'TTAA_G
Cuts at: 820 1017 3346 4076 820
Size: 197 2329 730 4968
Fragments arranged by size:
4968 2329 730 197
AflIII A'CryG_T
Cuts at: 1203 3791 4701 4902 7123 8100 1203
Size: 2588 910 201 2221 977 1327
Fragments arranged by size:
2588 2221 1327 977 910 201
AgeI A'CCGG_T
Cuts at: 7055 7055
Size: 8224
AhdI GACnn_n'nnGTC
Cuts at: 6953 6953
Size: 8224
AleI CACnn'nnGTG
Cuts at: 1166 3485 1166
Size: 2319 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8224
AlwNI CAG_nnn'CTG
Cuts at: 2986 3130 3461 4177 5440 5967 7539 2986
Size: 144 331 716 1263 527 1572 3671
Fragments arranged by size:
3671 1572 1263 716 527 331 144
ApaI G_GGCC'C
Cuts at: 2456 2456
Size: 8224
ApaLI G'TGCA_C
Cuts at: 6281 7437 6281
Size: 1156 7068
ApoI r'AATT_y
Cuts at: 1493 3682 3872 4150 4225 4286 5225 5533 5759
Size: 2189 190 278 75 61 939 308 226
Cuts at: 5759 5911 8020 1493
Size: 152 2109 1697
Fragments arranged by size:
2189 2109 1697 939 308 278 226 190
152 75 61
AseI AT'TA_AT
Cuts at: 160 1032 6778 160
Size: 872 5746 1606
Fragments arranged by size:
5746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
AvaI C'yCGr_G
Cuts at: 1870 1943 2449 4812 5549 1870
Size: 73 506 2363 737 4545
Fragments arranged by size:
4545 2363 737 506 73
BamHI G'GATC_C
Cuts at: 5554 5921 5554
Size: 367 7857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2795 4516 4649 5366
Size: 325 217 627 253 755 1721 133 717
Cuts at: 5366 5397 5545 7000 618
Size: 31 148 1455 1842
Fragments arranged by size:
1842 1721 1455 755 717 627 325 253
217 148 133 31
BanII G_rGCy'C
Cuts at: 1391 2456 3270 3522 3975 4335 5543 1391
Size: 1065 814 252 453 360 1208 4072
Fragments arranged by size:
4072 1208 1065 814 453 360 252
BbeI G_GCGC'C
Cuts at: 2044 2044
Size: 8224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3296 928
Size: 971 1397 5856
Fragments arranged by size:
5856 1397 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 4670 4704 4708 4742 4811
Size: 34 371 34 2328 34 4 34 69
Cuts at: 4811 4845 6415 6449 1903
Size: 34 1570 34 3678
Fragments arranged by size:
3678 2328 1570 371 69 34 34 34
34 34 34 4
BciVI GTATCCnnnnn_n'
Cuts at: 6116 7326 6116
Size: 1210 7014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6835 136
Size: 107 122 71 631 605 5163 1525
Fragments arranged by size:
5163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2496 8219 2496
Size: 5723 2501
BlpI GC'TnA_GC
Cuts at: 2015 5641 2015
Size: 3626 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2456 6285 7441 1790
Size: 666 3829 1156 2573
Fragments arranged by size:
3829 2573 1156 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5955 6913 442
Size: 416 53 83 399 24 4538 958 1753
Fragments arranged by size:
4538 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 3159 4687 2464
Size: 695 1528 6001
Fragments arranged by size:
6001 1528 695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4763 6884 1239
Size: 3524 2121 2579
Fragments arranged by size:
3524 2579 2121
Bpu10I CC'TnA_GC
Cuts at: 4848 4848
Size: 8224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6349 7214 7512 6349
Size: 865 298 7061
Fragments arranged by size:
7061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 5310 6887 882
Size: 617 672 3139 1577 2219
Fragments arranged by size:
3139 2219 1577 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 6015 493
Size: 711 2540 2271 2702
Fragments arranged by size:
2702 2540 2271 711
BsaBI GATnn'nnATC
Cuts at: 3176 8218 3176
Size: 5042 3182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 6031 6413 7048
Size: 53 83 186 936 508 3990 382 635
Cuts at: 7048 275
Size: 1451
Fragments arranged by size:
3990 1451 936 635 508 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4556 4948 6657 7055 7329 7476 7887 4556
Size: 392 1709 398 274 147 411 4893
Fragments arranged by size:
4893 1709 411 398 392 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2416 2446 2732 2762 1178
Size: 30 165 30 1013 30 286 30 6640
Fragments arranged by size:
6640 1013 286 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 4676 5267 1186
Size: 838 2652 591 4143
Fragments arranged by size:
4143 2652 838 591
BseYI C'CCAG_C
Cuts at: 2188 7427 7923 2188
Size: 5239 496 2489
Fragments arranged by size:
5239 2489 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2541 2980 4790 1163
Size: 573 805 439 1810 4597
Fragments arranged by size:
4597 1810 805 573 439
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 5674 6435 7463 1540
Size: 275 186 922 2751 761 1028 2301
Fragments arranged by size:
2751 2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3221 3270 3522 3975 5113 6285 6370 7441 3221
Size: 49 252 453 1138 1172 85 1071 4004
Fragments arranged by size:
4004 1172 1138 1071 453 252 85 49
BsmI GAATG_Cn'
Cuts at: 3805 5735 5828 3805
Size: 1930 93 6201
Fragments arranged by size:
6201 1930 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3782 3782
Size: 8224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 3221 3270 3522 3975 4335 5113
Size: 399 666 765 49 252 453 360 778
Cuts at: 5113 5543 6285 6370 7441 1391
Size: 430 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 765 742 666 453 430
399 360 252 85 49
BspEI T'CCGG_A
Cuts at: 7887 7887
Size: 8224
BspHI T'CATG_A
Cuts at: 5337 6113 7843 5337
Size: 776 1730 5718
Fragments arranged by size:
5718 1730 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2885 4915 5409 5579 844
Size: 2041 2030 494 170 3489
Fragments arranged by size:
3489 2041 2030 494 170
BsrBI CCG'CTC
Cuts at: 1394 1823 3000 4869 5382 6111 1394
Size: 429 1177 1869 513 729 3507
Fragments arranged by size:
3507 1869 1177 729 513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3500 4980 5127 6719 6893 65
Size: 1183 2252 1480 147 1592 174 1396
Fragments arranged by size:
2252 1592 1480 1396 1183 174 147
BsrFI r'CCGG_y
Cuts at: 1700 1810 5430 6868 7055 1700
Size: 110 3620 1438 187 2869
Fragments arranged by size:
3620 2869 1438 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8224
BssSI C'ACGA_G
Cuts at: 3483 6284 7296 3483
Size: 2801 1012 4411
Fragments arranged by size:
4411 2801 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2054 794
Size: 1260 6964
BstBI TT'CG_AA
Cuts at: 7028 7028
Size: 8224
BstEII G'GTnAC_C
Cuts at: 1184 2715 1184
Size: 1531 6693
BstXI CCAn_nnnn'nTGG
Cuts at: 3472 4513 3472
Size: 1041 7183
BstYI r'GATC_y
Cuts at: 1972 2496 5144 5554 5921 6306 6323 7775 7861
Size: 524 2648 410 367 385 17 1452 86
Cuts at: 7861 7873 8219 1972
Size: 12 346 1977
Fragments arranged by size:
2648 1977 1452 524 410 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4645 4896 5415 513
Size: 552 939 2641 251 519 3322
Fragments arranged by size:
3322 2641 939 552 519 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7485
Fragments arranged by size:
7485 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 4214 5740 6534 6554 2245
Size: 1969 1526 794 20 3915
Fragments arranged by size:
3915 1969 1526 794 20
ClaI AT'CG_AT
Cuts at: 1554 2328 1554
Size: 774 7450
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8189
DraI TTT'AAA
Cuts at: 3241 4224 4618 5528 3241
Size: 983 394 910 5937
Fragments arranged by size:
5937 983 910 394
DraIII CAC_nnn'GTG
Cuts at: 3263 3301 3263
Size: 38 8186
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2531 7231 809
Size: 507 1215 4700 1802
Fragments arranged by size:
4700 1802 1215 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2611 4410 4884
Size: 54 1425 50 275 18 781 1799 474
Cuts at: 4884 5262 5671 6560 8189 8
Size: 378 409 889 1629 43
Fragments arranged by size:
1799 1629 1425 889 781 474 409 378
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5671 1537
Size: 275 3859 4090
Fragments arranged by size:
4090 3859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2407 2463 3780 4770 6154 1353
Size: 1054 56 1317 990 1384 3423
Fragments arranged by size:
3423 1384 1317 1054 990 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6811 7185 7331 387
Size: 1064 138 5222 374 146 1280
Fragments arranged by size:
5222 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3268 3520 3973 3268
Size: 252 453 7519
Fragments arranged by size:
7519 453 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2487 4076 4763 4772 4976 6287
Size: 393 846 9 1589 687 9 204 1311
Cuts at: 6287 6884 7671 7939 1239
Size: 597 787 268 1524
Fragments arranged by size:
1589 1524 1311 846 787 687 597 393
268 204 9 9
EcoO109I rG'GnC_Cy
Cuts at: 2120 2147 2453 3057 3458 2120
Size: 27 306 604 401 6886
Fragments arranged by size:
6886 604 401 306 27
EcoRI G'AATT_C
Cuts at: 5533 5911 5533
Size: 378 7846
EcoRV GAT'ATC
Cuts at: 2326 2326
Size: 8224
FspI TGC'GCA
Cuts at: 6730 6730
Size: 8224
HaeII r_GCGC'y
Cuts at: 1678 2022 2044 4063 4325 7371 1678
Size: 344 22 2019 262 3046 2531
Fragments arranged by size:
3046 2531 2019 344 262 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2321 2353 2520
Size: 32 135 32 577 32 140 32 167
Cuts at: 2520 2552 2629 2661 4129 4161 4847 4879 5129
Size: 32 77 32 1468 32 686 32 250
Cuts at: 5129 5161 6865 6897 6939 6971 1373
Size: 32 1704 32 42 32 2626
Fragments arranged by size:
2626 1704 1468 686 577 250 167 140
135 77 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5568 5814 669
Size: 988 281 3630 246 3079
Fragments arranged by size:
3630 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8224
HpaI GTT'AAC
Cuts at: 5814 5814
Size: 8224
KasI G'GCGC_C
Cuts at: 2040 2040
Size: 8224
KpnI G_GTAC'C
Cuts at: 4520 5549 4520
Size: 1029 7195
MfeI C'AATT_G
Cuts at: 2697 5823 2697
Size: 3126 5098
MluI A'CGCG_T
Cuts at: 3791 8100 3791
Size: 4309 3915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2908 3746 5052 7338 7522 1305
Size: 1603 838 1306 2286 184 2007
Fragments arranged by size:
2286 2007 1603 1306 838 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2613 4412 4886 5264 10
Size: 54 1425 343 781 1799 474 378 2970
Fragments arranged by size:
2970 1799 1425 781 474 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3150 3485 4433 4655 5336 5372 6182
Size: 648 1984 335 948 222 681 36 810
Cuts at: 6182 6541 6700 518
Size: 359 159 2042
Fragments arranged by size:
2042 1984 948 810 681 648 359 335
222 159 36
MspA1I CmG'CkG
Cuts at: 1606 2485 4174 5101 5640 6317 7465 7710 1606
Size: 879 1689 927 539 677 1148 245 2120
Fragments arranged by size:
2120 1689 1148 927 879 677 539 245
NarI GG'CG_CC
Cuts at: 2041 2041
Size: 8224
NcoI C'CATG_G
Cuts at: 513 1065 2004 4645 513
Size: 552 939 2641 4092
Fragments arranged by size:
4092 2641 939 552
NdeI CA'TA_TG
Cuts at: 387 3112 387
Size: 2725 5499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8224
NotI GC'GGCC_GC
Cuts at: 5671 5671
Size: 8224
NruI TCG'CGA
Cuts at: 5120 5120
Size: 8224
NspI r_CATG'y
Cuts at: 1132 1229 2356 3908 4705 4906 5455 5582 7127
Size: 97 1127 1552 797 201 549 127 1545
Cuts at: 7127 1132
Size: 2229
Fragments arranged by size:
2229 1552 1545 1127 797 549 201 127
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8224
PciI A'CATG_T
Cuts at: 4701 4902 7123 4701
Size: 201 2221 5802
Fragments arranged by size:
5802 2221 201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 7012
PfoI T'CCnGG_A
Cuts at: 2755 3966 4188 4673 2755
Size: 1211 222 485 6306
Fragments arranged by size:
6306 1211 485 222
PmeI GTTT'AAAC
Cuts at: 5528 5528
Size: 8224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6293 6325 7806 7838 6293
Size: 32 1481 32 6679
Fragments arranged by size:
6679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2147 3057 2147
Size: 910 7314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8224
PsiI TTA'TAA
Cuts at: 5794 5794
Size: 8224
PspOMI G'GGCC_C
Cuts at: 2452 2452
Size: 8224
PstI C_TGCA'G
Cuts at: 830 2707 2780 3375 3702 5576 830
Size: 1877 73 595 327 1874 3478
Fragments arranged by size:
3478 1877 1874 595 327 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8224
PvuII CAG'CTG
Cuts at: 2485 4174 5101 2485
Size: 1689 927 5608
Fragments arranged by size:
5608 1689 927
SacI G_AGCT'C
Cuts at: 3270 3522 3975 3270
Size: 252 453 7519
Fragments arranged by size:
7519 453 252
SalI G'TCGA_C
Cuts at: 1936 5566 1936
Size: 3630 4594
SapI GCTCTTCn'nnn_
Cuts at: 2463 2463
Size: 8224
SbfI CC_TGCA'GG
Cuts at: 5576 5576
Size: 8224
ScaI AGT'ACT
Cuts at: 3030 6472 8184 3030
Size: 3442 1712 3070
Fragments arranged by size:
3442 3070 1712
SfcI C'TryA_G
Cuts at: 826 1045 2703 2776 2851 3371 3663 3698 3984
Size: 219 1658 73 75 520 292 35 286
Cuts at: 3984 5149 5290 5572 6707 7388 7579 826
Size: 1165 141 282 1135 681 191 1471
Fragments arranged by size:
1658 1471 1165 1135 681 520 292 286
282 219 191 141 75 73 35
SfoI GGC'GCC
Cuts at: 2042 2042
Size: 8224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8224
SmaI CCC'GGG
Cuts at: 1872 4814 5551 1872
Size: 2942 737 4545
Fragments arranged by size:
4545 2942 737
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3346 4076 6328 7229 7491 820
Size: 197 926 1403 730 2252 901 262 1553
Fragments arranged by size:
2252 1553 1403 926 901 730 262 197
SnaBI TAC'GTA
Cuts at: 493 6015 493
Size: 5522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8224
SphI G_CATG'C
Cuts at: 1132 3908 5455 5582 1132
Size: 2776 1547 127 3774
Fragments arranged by size:
3774 2776 1547 127
SspI AAT'ATT
Cuts at: 5 52 6148 5
Size: 47 6096 2081
Fragments arranged by size:
6096 2081 47
StuI AGG'CCT
Cuts at: 1517 2477 3195 4100 1517
Size: 960 718 905 5641
Fragments arranged by size:
5641 960 905 718
StyI C'CwwG_G
Cuts at: 513 1065 2004 4239 4645 5663 513
Size: 552 939 2235 406 1018 3074
Fragments arranged by size:
3074 2235 1018 939 552 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2937 3771 6251 6268 6421 6606 7051
Size: 1255 1357 834 2480 17 153 185 445
Cuts at: 7051 325
Size: 1498
Fragments arranged by size:
2480 1498 1357 1255 834 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3028 3710 4337
Size: 274 80 33 51 1443 1051 682 627
Cuts at: 4337 6470 8182 96
Size: 2133 1712 138
Fragments arranged by size:
2133 1712 1443 1051 682 627 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 8184 8216 2416
Size: 32 5736 32 2424
Fragments arranged by size:
5736 2424 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8224
XbaI T'CTAG_A
Cuts at: 5560 5560
Size: 8224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4510 4894 4510
Size: 384 7840
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8224
XmaI C'CCGG_G
Cuts at: 1870 4812 5549 1870
Size: 2942 737 4545
Fragments arranged by size:
4545 2942 737
XmnI GAAnn'nnTTC
Cuts at: 4937 6353 4937
Size: 1416 6808
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6032 276
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoNI FalI FseI FspAI NaeI NgoMIV NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI