Restriction map for
pFN21AB9993
(Circular) (Six-base) MAPSORT of: pFN21AB9993.seq Check: 9625 from: 1 to: 6457
pFN21AB9993 6457 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3096 3730 3800 1937
Size: 1159 634 70 4594
Fragments arranged by size:
4594 1159 634 70
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4520 5904 6172 1632
Size: 2888 1384 268 1917
Fragments arranged by size:
2888 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3248 1184
Size: 2064 4393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 6425
AlwNI CAG_nnn'CTG
Cuts at: 3131 4200 5772 3131
Size: 1069 1572 3816
Fragments arranged by size:
3816 1572 1069
ApaI G_GGCC'C
Cuts at: 2136 3342 2136
Size: 1206 5251
ApaLI G'TGCA_C
Cuts at: 3366 4514 5670 3366
Size: 1148 1156 4153
Fragments arranged by size:
4153 1156 1148
ApoI r'AATT_y
Cuts at: 1493 2681 3766 3992 4144 6253 1493
Size: 1188 1085 226 152 2109 1697
Fragments arranged by size:
2109 1697 1188 1085 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2370 2477 3064 3372 3782 1870
Size: 73 427 107 587 308 410 4545
Fragments arranged by size:
4545 587 427 410 308 107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2491 2524 2491
Size: 33 6424
BamHI G'GATC_C
Cuts at: 2803 3787 4154 2803
Size: 984 367 5106
Fragments arranged by size:
5106 984 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 3778 5233 618
Size: 325 217 627 445 1546 1455 1842
Fragments arranged by size:
1842 1546 1455 627 445 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 3342 3468 3776 1391
Size: 745 1206 126 308 4072
Fragments arranged by size:
4072 1206 745 308 126
BbeI G_GCGC'C
Cuts at: 2236 2236
Size: 6457
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5486
BbvCI CC'TCA_GC
Cuts at: 3501 3501
Size: 6457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2873 2907 4648 4682 1903
Size: 34 936 34 1741 34 3678
Fragments arranged by size:
3678 1741 936 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4349 5559 4349
Size: 1210 5247
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 5068 136
Size: 107 122 71 631 605 550 2846 1525
Fragments arranged by size:
2846 1525 631 605 550 122 107 71
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 2651 3007 3447 3485 3874 2651
Size: 356 440 38 389 5234
Fragments arranged by size:
5234 440 389 356 38
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2539 3342 3370 4518 5674 1790
Size: 346 403 803 28 1148 1156 2573
Fragments arranged by size:
2573 1156 1148 803 403 346 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3424 4188 5146
Size: 416 53 83 399 24 2007 764 958
Cuts at: 5146 442
Size: 1753
Fragments arranged by size:
2007 1753 958 764 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2312 2507 2894 3108 5117 1239
Size: 848 225 195 387 214 2009 2579
Fragments arranged by size:
2579 2009 848 387 225 214 195
Bpu10I CC'TnA_GC
Cuts at: 2610 3501 3597 2610
Size: 891 96 5470
Fragments arranged by size:
5470 891 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4582 5447 5745 4582
Size: 865 298 5294
Fragments arranged by size:
5294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5120 882
Size: 617 3621 2219
Fragments arranged by size:
3621 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4248 493
Size: 711 3044 2702
Fragments arranged by size:
3044 2702 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2233 4264 4646 5281
Size: 53 83 186 936 700 2031 382 635
Cuts at: 5281 275
Size: 1451
Fragments arranged by size:
2031 1451 936 700 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2043 2806 4890 5288 5562 5709 6120 2043
Size: 763 2084 398 274 147 411 2380
Fragments arranged by size:
2380 2084 763 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2310 2340 3455 3485 1178
Size: 30 165 30 907 30 1115 30 4150
Fragments arranged by size:
4150 1115 907 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2136 2315 2414 2641 2683 3035 3038
Size: 928 22 179 99 227 42 352 3
Cuts at: 3038 1186
Size: 4605
Fragments arranged by size:
4605 928 352 227 179 99 42 22
3
BseYI C'CCAG_C
Cuts at: 2225 3164 3335 5660 6156 2225
Size: 939 171 2325 496 2526
Fragments arranged by size:
2526 2325 939 496 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3129 1163
Size: 573 1393 4491
Fragments arranged by size:
4491 1393 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3907 4668 5696 1540
Size: 275 186 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2183 3370 3548 3573 4518 4603 5674 2183
Size: 1187 178 25 945 85 1071 2966
Fragments arranged by size:
2966 1187 1071 945 178 85 25
BsmI GAATG_Cn'
Cuts at: 2697 3968 4061 2697
Size: 1271 93 5093
Fragments arranged by size:
5093 1271 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2716 2716
Size: 6457
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2183 2539 3342 3370 3468 3548
Size: 399 346 47 356 803 28 98 80
Cuts at: 3548 3573 3776 4518 4603 5674 1391
Size: 25 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 803 742 399 356 346 203
98 85 80 47 28 25
BspEI T'CCGG_A
Cuts at: 2043 2806 6120 2043
Size: 763 3314 2380
Fragments arranged by size:
3314 2380 763
BspHI T'CATG_A
Cuts at: 2980 4346 6076 2980
Size: 1366 1730 3361
Fragments arranged by size:
3361 1730 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3812 844
Size: 2968 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2650 3122 3748 4344 1394
Size: 429 827 472 626 596 3507
Fragments arranged by size:
3507 827 626 596 472 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3182 4952 5126 65
Size: 1183 1934 1770 174 1396
Fragments arranged by size:
1934 1770 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3489 5101 5288 1700
Size: 110 1679 1612 187 2869
Fragments arranged by size:
2869 1679 1612 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3152 794
Size: 2358 4099
BstBI TT'CG_AA
Cuts at: 3399 5261 3399
Size: 1862 4595
BstEII G'GTnAC_C
Cuts at: 1184 2563 2971 1184
Size: 1379 408 4670
Fragments arranged by size:
4670 1379 408
BstXI CCAn_nnnn'nTGG
Cuts at: 2584 3172 2584
Size: 588 5869
BstYI r'GATC_y
Cuts at: 1972 2008 2803 3211 3787 4154 4539 4556 6008
Size: 36 795 408 576 367 385 17 1452
Cuts at: 6008 6094 6106 6452 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 795 576 408 385 367 346
86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2951 3621 2207
Size: 744 670 5043
Fragments arranged by size:
5043 744 670
BtgI C'CryG_G
Cuts at: 513 1065 2004 2413 3441 3475 513
Size: 552 939 409 1028 34 3495
Fragments arranged by size:
3495 1028 939 552 409 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2046 2743 487
Size: 683 56 820 697 4201
Fragments arranged by size:
4201 820 697 683 56
BtsI GCAGTG_nn'
Cuts at: 3141 3330 3370 3506 3973 4767 4787 3141
Size: 189 40 136 467 794 20 4811
Fragments arranged by size:
4811 794 467 189 136 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2329 2364 529
Size: 35 1765 35 4622
Fragments arranged by size:
4622 1765 35 35
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2736 2917
Size: 54 1425 50 275 18 384 522 181
Cuts at: 2917 3559 3904 4793 6422 8
Size: 642 345 889 1629 43
Fragments arranged by size:
1629 1425 889 642 522 384 345 275
181 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3024 3414 4387 1353
Size: 1671 390 973 3423
Fragments arranged by size:
3423 1671 973 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5044 5418 5564 387
Size: 1064 138 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2312 2507 2894 3108 4520 5117
Size: 393 455 225 195 387 214 1412 597
Cuts at: 5117 5904 6172 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1412 787 597 455 393 387 268
225 214 195
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 3308 2327
Size: 981 5476
EcoO109I rG'GnC_Cy
Cuts at: 2132 2312 2474 2518 3339 3376 2132
Size: 180 162 44 821 37 5213
Fragments arranged by size:
5213 821 180 162 44 37
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3425 3425
Size: 6457
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 2236 5604 1678
Size: 558 3368 2531
Fragments arranged by size:
3368 2531 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 3223 3255 3266
Size: 32 135 32 470 32 1149 32 11
Cuts at: 3266 3298 3628 3660 5098 5130 5172 5204 1373
Size: 32 330 32 1438 32 42 32 2626
Fragments arranged by size:
2626 1438 1149 470 330 135 42 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3292 3801 4047 669
Size: 988 281 1354 509 246 3079
Fragments arranged by size:
3079 1354 988 509 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KasI G'GCGC_C
Cuts at: 2232 2232
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5571 5755 1305
Size: 4266 184 2007
Fragments arranged by size:
4266 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 2919 3561 10
Size: 54 1425 343 384 703 642 2906
Fragments arranged by size:
2906 1425 703 642 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4415 4774 4933 518
Size: 648 3249 359 159 2042
Fragments arranged by size:
3249 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2267 3873 4550 5698 5943 1606
Size: 661 1606 677 1148 245 2120
Fragments arranged by size:
2120 1606 1148 677 661 245
NaeI GCC'GGC
Cuts at: 3491 3491
Size: 6457
NarI GG'CG_CC
Cuts at: 2233 2233
Size: 6457
NcoI C'CATG_G
Cuts at: 513 1065 2004 3441 3475 513
Size: 552 939 1437 34 3495
Fragments arranged by size:
3495 1437 939 552 34
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NgoMIV G'CCGG_C
Cuts at: 3489 3489
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 2776 3815 5360 1132
Size: 97 1547 1039 1545 2229
Fragments arranged by size:
2229 1547 1545 1039 97
PasI CC'CwG_GG
Cuts at: 1348 2128 2237 1348
Size: 780 109 5568
Fragments arranged by size:
5568 780 109
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2872 1108
Size: 1764 4693
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4526 4558 6039 6071 3455
Size: 32 1039 32 1481 32 3841
Fragments arranged by size:
3841 1481 1039 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2474 2518 3376 2474
Size: 44 858 5555
Fragments arranged by size:
5555 858 44
PshAI GACnn'nnGTC
Cuts at: 1613 2163 2561 1613
Size: 550 398 5509
Fragments arranged by size:
5509 550 398
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PspOMI G'GGCC_C
Cuts at: 2132 3338 2132
Size: 1206 5251
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3714 3746 3714
Size: 32 6425
PstI C_TGCA'G
Cuts at: 830 3516 3809 830
Size: 2686 293 3478
Fragments arranged by size:
3478 2686 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2267 2267
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SanDI GG'GwC_CC
Cuts at: 2474 2474
Size: 6457
SapI GCTCTTCn'nnn_
Cuts at: 3024 3414 3024
Size: 390 6067
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 3050 4705 6417 3050
Size: 1655 1712 3090
Fragments arranged by size:
3090 1712 1655
SfcI C'TryA_G
Cuts at: 826 1045 2638 3189 3512 3731 3805 4940 5621
Size: 219 1593 551 323 219 74 1135 681
Cuts at: 5621 5812 826
Size: 191 1471
Fragments arranged by size:
1593 1471 1135 681 551 323 219 219
191 74
SfoI GGC'GCC
Cuts at: 2234 2234
Size: 6457
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 2479 3066 3374 3784 1872
Size: 607 587 308 410 4545
Fragments arranged by size:
4545 607 587 410 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4561 5462 5724 820
Size: 197 926 2618 901 262 1553
Fragments arranged by size:
2618 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 2924 1517
Size: 1407 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 3081 3441 3475 3896 513
Size: 552 939 1077 360 34 421 3074
Fragments arranged by size:
3074 1077 939 552 421 360 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2977 3163 3317 4484 4501 4654 4839
Size: 1255 1397 186 154 1167 17 153 185
Cuts at: 4839 5284 325
Size: 445 1498
Fragments arranged by size:
1498 1397 1255 1167 445 186 185 154
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 3048 4703
Size: 274 80 33 51 1443 54 1017 1655
Cuts at: 4703 6415 96
Size: 1712 138
Fragments arranged by size:
1712 1655 1443 1017 274 138 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6417 6449 6417
Size: 32 6425
Tth111I GACn'n_nGTC
Cuts at: 1316 2465 1316
Size: 1149 5308
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 2477 3064 3372 3782 1870
Size: 607 587 308 410 4545
Fragments arranged by size:
4545 607 587 410 308
XmnI GAAnn'nnTTC
Cuts at: 3395 4586 3395
Size: 1191 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SacI SacII SexAI SfiI SrfI
SwaI XcmI