Restriction map for
pFN21AB9989
(Circular) (Six-base) MAPSORT of: pFN21AB9989.seq Check: 1946 from: 1 to: 6442
pFN21AB9989 6442 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 4505 5889 6157 1632
Size: 981 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 981 268
AflII C'TTAA_G
Cuts at: 820 1017 3071 820
Size: 197 2054 4191
Fragments arranged by size:
4191 2054 197
AflIII A'CryG_T
Cuts at: 1203 2728 3707 5341 6318 1203
Size: 1525 979 1634 977 1327
Fragments arranged by size:
1634 1525 1327 979 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2334 1184
Size: 1150 5292
AlwNI CAG_nnn'CTG
Cuts at: 4185 5757 4185
Size: 1572 4870
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2844 3143 3504 3751 3977 4129 6238 1493
Size: 1351 299 361 247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1351 361 299 247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3761 1391
Size: 2370 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 3623 928
Size: 971 824 900 3747
Fragments arranged by size:
3747 971 900 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2151 2185 4633 4667 1903
Size: 34 214 34 2448 34 3678
Fragments arranged by size:
3678 2448 214 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BfrBI ATG'CAT
Cuts at: 2417 2561 2417
Size: 144 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5053 136
Size: 107 122 71 631 605 337 3044 1525
Fragments arranged by size:
3044 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 4503 5659 1790
Size: 2713 1156 2573
Fragments arranged by size:
2713 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4173 5131
Size: 416 53 83 399 24 1958 798 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
1958 1753 958 798 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3434 1056
Size: 2378 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 5102 1239
Size: 990 2873 2579
Fragments arranged by size:
2873 2579 990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 3728 5105 882
Size: 617 756 1473 1377 2219
Fragments arranged by size:
2219 1473 1377 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 2439 6436 2439
Size: 3997 2445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2874 4249 4631 5266
Size: 53 83 186 936 1341 1375 382 635
Cuts at: 5266 275
Size: 1451
Fragments arranged by size:
1451 1375 1341 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2176 4875 5273 5547 5694 6105 2176
Size: 2699 398 274 147 411 2513
Fragments arranged by size:
2699 2513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3219 3249 3397 3427 1178
Size: 30 165 30 1816 30 148 30 4193
Fragments arranged by size:
4193 1816 165 148 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 1186
Size: 1540 4902
BseYI C'CCAG_C
Cuts at: 3182 5645 6141 3182
Size: 2463 496 3483
Fragments arranged by size:
3483 2463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2901 3072 3257 3690 1163
Size: 573 1165 171 185 433 3915
Fragments arranged by size:
3915 1165 573 433 185 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3311 3475 4503 4588 5659 3311
Size: 164 1028 85 1071 4094
Fragments arranged by size:
4094 1071 1028 164 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
BsmBI CGTCTCn'nnnn_
Cuts at: 2154 2154
Size: 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3311 3475 3761 4503 4588 5659 1391
Size: 399 1521 164 286 742 85 1071 2174
Fragments arranged by size:
2174 1521 1071 742 399 286 164 85
BspEI T'CCGG_A
Cuts at: 2176 6105 2176
Size: 3929 2513
BspHI T'CATG_A
Cuts at: 3649 3685 4331 6061 3649
Size: 36 646 1730 4030
Fragments arranged by size:
4030 1730 646 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 3797 844
Size: 1904 1049 3489
Fragments arranged by size:
3489 1904 1049
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3215 4937 5111 65
Size: 1183 1967 1722 174 1396
Fragments arranged by size:
1967 1722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5086 5273 1700
Size: 110 3276 187 2869
Fragments arranged by size:
3276 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3394 3533 794
Size: 2600 139 3703
Fragments arranged by size:
3703 2600 139
BstBI TT'CG_AA
Cuts at: 3220 5246 3220
Size: 2026 4416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 3772 4139 4524 4541 5993 6079 6091 6437
Size: 1800 367 385 17 1452 86 12 346
Cuts at: 6437 1972
Size: 1977
Fragments arranged by size:
1977 1800 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2778 2778
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 2588 2928 513
Size: 552 1523 340 4027
Fragments arranged by size:
4027 1523 552 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 2169 3408 3678 3958 4752 4772 2163
Size: 6 1239 270 280 794 20 3833
Fragments arranged by size:
3833 1239 794 280 270 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2583 2618 529
Size: 35 2019 35 4353
Fragments arranged by size:
4353 2019 35 35
DraI TTT'AAA
Cuts at: 2548 3643 3746 2548
Size: 1095 103 5244
Fragments arranged by size:
5244 1095 103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2228 5449 809
Size: 507 912 3221 1802
Fragments arranged by size:
3221 1802 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2905 3889
Size: 54 1425 50 275 18 198 877 984
Cuts at: 3889 4778 6407 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 984 889 877 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 2691 4372 1353
Size: 1278 60 1681 3423
Fragments arranged by size:
3423 1681 1278 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2613 4505 5102 5889 6157 1239
Size: 393 597 384 1892 597 787 268 1524
Fragments arranged by size:
1892 1524 787 597 597 393 384 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3061 3061
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2760 2760
Size: 6442
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
EcoRV GAT'ATC
Cuts at: 3016 3349 3016
Size: 333 6109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2412 2740 2412
Size: 328 6114
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2974 5589 1678
Size: 1296 2615 2531
Fragments arranged by size:
2615 2531 1296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 3397 3429 5083
Size: 32 135 32 436 32 1357 32 1654
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1654 1357 436 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3268 3786 4032 669
Size: 988 281 867 463 518 246 3079
Fragments arranged by size:
3079 988 867 518 463 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5556 5740 1305
Size: 4251 184 2007
Fragments arranged by size:
4251 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2907 10
Size: 54 1425 343 1075 3545
Fragments arranged by size:
3545 1425 1075 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 3684 4400 4759 4918 518
Size: 648 1198 1320 716 359 159 2042
Fragments arranged by size:
2042 1320 1198 716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 3858 4535 5683 5928 1606
Size: 489 1763 677 1148 245 2120
Fragments arranged by size:
2120 1763 1148 677 489 245
NcoI C'CATG_G
Cuts at: 513 1065 2588 2928 513
Size: 552 1523 340 4027
Fragments arranged by size:
4027 1523 552 340
NdeI CA'TA_TG
Cuts at: 387 2407 3519 387
Size: 2020 1112 3310
Fragments arranged by size:
3310 2020 1112
NheI G'CTAG_C
Cuts at: 1052 3430 1052
Size: 2378 4064
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 2419 2563 2419
Size: 144 6298
NspI r_CATG'y
Cuts at: 1132 1229 2732 3438 3711 3800 5345 1132
Size: 97 1503 706 273 89 1545 2229
Fragments arranged by size:
2229 1545 1503 706 273 97 89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 2728 3707 5341 2728
Size: 979 1634 3829
Fragments arranged by size:
3829 1634 979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3445 1108
Size: 2337 4105
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2760 2760
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 3034 3403 3794 830
Size: 2204 369 391 3478
Fragments arranged by size:
3478 2204 391 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SapI GCTCTTCn'nnn_
Cuts at: 2631 2631
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2469 3030 3399 3631 3790 4925 5606
Size: 219 1424 561 369 232 159 1135 681
Cuts at: 5606 5797 826
Size: 191 1471
Fragments arranged by size:
1471 1424 1135 681 561 369 232 219
191 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3071 4546 5447 5709 820
Size: 197 926 1128 1475 901 262 1553
Fragments arranged by size:
1553 1475 1128 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3438 3800 1132
Size: 2306 362 3774
Fragments arranged by size:
3774 2306 362
SspI AAT'ATT
Cuts at: 5 52 2569 4366 5
Size: 47 2517 1797 2081
Fragments arranged by size:
2517 2081 1797 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 2588 2928 3881 513
Size: 552 1523 340 953 3074
Fragments arranged by size:
3074 1523 953 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4469 4486 4639 4824 5269 325
Size: 1255 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3366 4688 6400
Size: 274 80 33 51 1443 1389 1322 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
1712 1443 1389 1322 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 6402 6434 3219
Size: 32 3151 32 3227
Fragments arranged by size:
3227 3151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3409 1316
Size: 2093 4349
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3280 3423 3280
Size: 143 6299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI