Restriction map for
pFN21AB9987
(Circular) (Six-base) MAPSORT of: pFN21AB9987.seq Check: 2627 from: 1 to: 6427
pFN21AB9987 6427 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2282 2143
Size: 139 6288
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3618 3770 1937
Size: 1681 152 4594
Fragments arranged by size:
4594 1681 152
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 3695 4490 5874 6142 1632
Size: 1955 108 795 1384 268 1917
Fragments arranged by size:
1955 1917 1384 795 268 108
AfeI AGC'GCT
Cuts at: 3377 3377
Size: 6427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 4903
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 2089 2316 2782 2926 2991 3161 4170 5742 2089
Size: 227 466 144 65 170 1009 1572 2774
Fragments arranged by size:
2774 1572 1009 466 227 170 144 65
ApaLI G'TGCA_C
Cuts at: 2772 4484 5640 2772
Size: 1712 1156 3559
Fragments arranged by size:
3559 1712 1156
ApoI r'AATT_y
Cuts at: 1493 3281 3541 3736 3962 4114 6223 1493
Size: 1788 260 195 226 152 2109 1697
Fragments arranged by size:
2109 1788 1697 260 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3752 1870
Size: 73 934 875 4545
Fragments arranged by size:
4545 934 875 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 2854 3048 3748 5203
Size: 325 217 627 371 696 194 700 1455
Cuts at: 5203 618
Size: 1842
Fragments arranged by size:
1842 1455 700 696 627 371 325 217
194
BanII G_rGCy'C
Cuts at: 1391 2404 3746 1391
Size: 1013 1342 4072
Fragments arranged by size:
4072 1342 1013
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 928
Size: 971 414 5042
Fragments arranged by size:
5042 971 414
BbvCI CC'TCA_GC
Cuts at: 2013 2530 2013
Size: 517 5910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4618 4652 1903
Size: 34 50 34 2597 34 3678
Fragments arranged by size:
3678 2597 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2269 2772 4319 5529 2269
Size: 503 1547 1210 3167
Fragments arranged by size:
3167 1547 1210 503
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3598 5038 136
Size: 107 122 71 631 605 1926 1440 1525
Fragments arranged by size:
1926 1525 1440 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2776 3116 3594 4488 5644 1790
Size: 986 340 478 894 1156 2573
Fragments arranged by size:
2573 1156 986 894 478 340
BmgBI CAC'GTC
Cuts at: 1318 3439 1318
Size: 2121 4306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4158 5116 442
Size: 416 53 83 399 24 2741 958 1753
Fragments arranged by size:
2741 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2952 3171 5087 1239
Size: 1076 637 219 1916 2579
Fragments arranged by size:
2579 1916 1076 637 219
Bpu10I CC'TnA_GC
Cuts at: 2013 2530 2013
Size: 517 5910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2199 2271 4552 5417 5715 2199
Size: 72 2281 865 298 2911
Fragments arranged by size:
2911 2281 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3121 5090 882
Size: 617 1622 1969 2219
Fragments arranged by size:
2219 1969 1622 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 3404 6421 3404
Size: 3017 3410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2201 4860 5258 5532 5679 6090 2201
Size: 2659 398 274 147 411 2538
Fragments arranged by size:
2659 2538 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 2925 2955 1178
Size: 30 165 30 780 30 712 30 4650
Fragments arranged by size:
4650 780 712 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 3675 1186
Size: 815 1674 3938
Fragments arranged by size:
3938 1674 815
BseYI C'CCAG_C
Cuts at: 2574 2634 2984 3141 3154 5630 6126 2574
Size: 60 350 157 13 2476 496 2875
Fragments arranged by size:
2875 2476 496 350 157 60 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 2936 2981 1163
Size: 573 424 776 45 4609
Fragments arranged by size:
4609 776 573 424 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2363 2776 3094 3564 4488 4573 5644 2363
Size: 413 318 470 924 85 1071 3146
Fragments arranged by size:
3146 1071 924 470 413 318 85
BsmI GAATG_Cn'
Cuts at: 2043 3938 4031 2043
Size: 1895 93 4439
Fragments arranged by size:
4439 1895 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2363 2404 2776 3094 3116 3564 3594
Size: 399 573 41 372 318 22 448 30
Cuts at: 3594 3746 4488 4573 5644 1391
Size: 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 448 399 372 318
152 85 41 30 22
BspEI T'CCGG_A
Cuts at: 2201 6090 2201
Size: 3889 2538
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 2257 2282 3782 844
Size: 1299 114 25 1500 3489
Fragments arranged by size:
3489 1500 1299 114 25
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2905 5071 5258 1700
Size: 110 1095 2166 187 2869
Fragments arranged by size:
2869 2166 1095 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2926 2959 794
Size: 2132 33 4262
Fragments arranged by size:
4262 2132 33
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 2269 1184
Size: 1085 5342
BstXI CCAn_nnnn'nTGG
Cuts at: 2834 2834
Size: 6427
BstYI r'GATC_y
Cuts at: 1972 2602 3757 4124 4509 4526 5978 6064 6076
Size: 630 1155 367 385 17 1452 86 12
Cuts at: 6076 6422 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1155 630 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3383 3383
Size: 6427
BtgI C'CryG_G
Cuts at: 513 1065 2810 3689 513
Size: 552 1745 879 3251
Fragments arranged by size:
3251 1745 879 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 3943 4737 4757 3943
Size: 794 20 5613
Fragments arranged by size:
5613 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DraIII CAC_nnn'GTG
Cuts at: 2854 2959 2854
Size: 105 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2488 2863 3874
Size: 54 1425 50 275 18 658 375 1011
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1011 889 658 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2997 3569 3666 4357 1353
Size: 759 885 572 97 691 3423
Fragments arranged by size:
3423 885 759 691 572 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2952 3171 3587 3695 4490 5087
Size: 393 683 637 219 416 108 795 597
Cuts at: 5087 5874 6142 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 683 637 597 416 393
268 219 108
EcoO109I rG'GnC_Cy
Cuts at: 2673 2707 3695 2673
Size: 34 988 5405
Fragments arranged by size:
5405 988 34
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
EcoRV GAT'ATC
Cuts at: 3358 3358
Size: 6427
FspI TGC'GCA
Cuts at: 2282 4933 2282
Size: 2651 3776
HaeII r_GCGC'y
Cuts at: 1678 3379 3450 5574 1678
Size: 1701 71 2124 2531
Fragments arranged by size:
2531 2124 1701 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2770 2802 5068
Size: 32 135 32 794 32 372 32 2266
Cuts at: 5068 5100 5142 5174 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2266 794 372 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2343 748
Size: 1595 4832
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 3321 4026 3321
Size: 705 5722
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 5541 5725 1305
Size: 1072 3164 184 2007
Fragments arranged by size:
3164 2007 1072 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 2865 10
Size: 54 1425 343 658 375 3572
Fragments arranged by size:
3572 1425 658 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2690 3266 3557 4385 4744 4903
Size: 648 882 642 576 291 828 359 159
Cuts at: 4903 518
Size: 2042
Fragments arranged by size:
2042 882 828 648 642 576 359 291
159
MspA1I CmG'CkG
Cuts at: 1606 2251 2471 2665 3158 3460 3624 3843 4520
Size: 645 220 194 493 302 164 219 677
Cuts at: 4520 5668 5913 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 493 302 245 220
219 194 164
NaeI GCC'GGC
Cuts at: 2907 2907
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 2810 513
Size: 552 1745 4130
Fragments arranged by size:
4130 1745 552
NdeI CA'TA_TG
Cuts at: 387 2860 387
Size: 2473 3954
NgoMIV G'CCGG_C
Cuts at: 2905 2905
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2772 2807 3590 3785 5330 1132
Size: 97 1543 35 783 195 1545 2229
Fragments arranged by size:
2229 1545 1543 783 195 97 35
PasI CC'CwG_GG
Cuts at: 1348 2991 1348
Size: 1643 4784
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 2991 1108
Size: 1452 431 4544
Fragments arranged by size:
4544 1452 431
PfoI T'CCnGG_A
Cuts at: 2833 2833
Size: 6427
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 2707
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspXI vC'TCGA_Gb
Cuts at: 2877 2877
Size: 6427
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 6395
PstI C_TGCA'G
Cuts at: 830 2321 2368 2413 3121 3779 830
Size: 1491 47 45 708 658 3478
Fragments arranged by size:
3478 1491 708 658 47 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2665 3158 3460 3624 2665
Size: 493 302 164 5468
Fragments arranged by size:
5468 493 302 164
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SapI GCTCTTCn'nnn_
Cuts at: 3569 3569
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SexAI A'CCwGG_T
Cuts at: 2850 2850
Size: 6427
SfcI C'TryA_G
Cuts at: 826 1045 2317 2364 2409 3117 3619 3775 4910
Size: 219 1272 47 45 708 502 156 1135
Cuts at: 4910 5591 5782 826
Size: 681 191 1471
Fragments arranged by size:
1471 1272 1135 708 681 502 219 191
156 47 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2214 2286 2877 4531 5432 5694
Size: 197 926 271 72 591 1654 901 262
Cuts at: 5694 820
Size: 1553
Fragments arranged by size:
1654 1553 926 901 591 271 262 197
72
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 2108 4351 5
Size: 47 2056 2243 2081
Fragments arranged by size:
2243 2081 2056 47
StuI AGG'CCT
Cuts at: 1517 2334 2598 2949 1517
Size: 817 264 351 4995
Fragments arranged by size:
4995 817 351 264
StyI C'CwwG_G
Cuts at: 513 1065 2541 2810 3044 3105 3723 3866 513
Size: 552 1476 269 234 61 618 143 3074
Fragments arranged by size:
3074 1476 618 552 269 234 143 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2176 2194 2343 2346 4454 4471 4624
Size: 1255 596 18 149 3 2108 17 153
Cuts at: 4624 4809 5254 325
Size: 185 445 1498
Fragments arranged by size:
2108 1498 1255 596 445 185 153 149
18 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3492 4673 6385
Size: 274 80 33 51 1443 1515 1181 1712
Cuts at: 6385 96
Size: 138
Fragments arranged by size:
1712 1515 1443 1181 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 2925 2957 6387 6419 2183
Size: 32 710 32 3430 32 2191
Fragments arranged by size:
3430 2191 710 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2808 2858 3712 2808
Size: 50 854 5523
Fragments arranged by size:
5523 854 50
XhoI C'TCGA_G
Cuts at: 1943 2877 1943
Size: 934 5493
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI