Restriction map for
pFN21AB9982
(Circular) (Six-base) MAPSORT of: pFN21AB9982.seq Check: 7089 from: 1 to: 6832
pFN21AB9982 6832 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2685 2685
Size: 6832
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2034 4642 278
Size: 53 83 186 936 498 2608 2468
Fragments arranged by size:
2608 2468 936 498 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2068 2217 3206 4175 1937
Size: 131 149 989 969 4594
Fragments arranged by size:
4594 989 969 149 131
Acc65I G'GTAC_C
Cuts at: 3149 4153 3149
Size: 1004 5828
AclI AA'CG_TT
Cuts at: 1563 4959 5332 1563
Size: 3396 373 3063
Fragments arranged by size:
3396 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3196 4089 4895 6279 6547 1632
Size: 1564 893 806 1384 268 1917
Fragments arranged by size:
1917 1564 1384 893 806 268
AfeI AGC'GCT
Cuts at: 2894 2894
Size: 6832
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6635
AflIII A'CryG_T
Cuts at: 1203 2919 5731 6708 1203
Size: 1716 2812 977 1327
Fragments arranged by size:
2812 1716 1327 977
AgeI A'CCGG_T
Cuts at: 5663 5663
Size: 6832
AhdI GACnn_n'nnGTC
Cuts at: 4114 5561 4114
Size: 1447 5385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6832
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6832
AlwNI CAG_nnn'CTG
Cuts at: 2626 4575 6147 2626
Size: 1949 1572 3311
Fragments arranged by size:
3311 1949 1572
ApaI G_GGCC'C
Cuts at: 3019 3019
Size: 6832
ApaLI G'TGCA_C
Cuts at: 2419 3104 4889 6045 2419
Size: 685 1785 1156 3206
Fragments arranged by size:
3206 1785 1156 685
ApoI r'AATT_y
Cuts at: 1493 2342 2450 4141 4367 4519 6628 1493
Size: 849 108 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 849 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 5386 160
Size: 872 4354 1606
Fragments arranged by size:
4354 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
AvaI C'yCGr_G
Cuts at: 1870 1943 4157 1870
Size: 73 2214 4545
Fragments arranged by size:
4545 2214 73
BamHI G'GATC_C
Cuts at: 2782 2927 4121 4162 4529 2782
Size: 145 1194 41 367 5085
Fragments arranged by size:
5085 1194 367 145 41
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 3149 4153 5608 618
Size: 325 217 627 376 986 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 986 627 376 325 217
BanII G_rGCy'C
Cuts at: 1391 3019 3944 4019 4151 1391
Size: 1628 925 75 132 4072
Fragments arranged by size:
4072 1628 925 132 75
BbeI G_GCGC'C
Cuts at: 2167 2167
Size: 6832
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3858 928
Size: 971 1959 3902
Fragments arranged by size:
3902 1959 971
BbvCI CC'TCA_GC
Cuts at: 2402 3804 2402
Size: 1402 5430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3308 3342 5023 5057 1903
Size: 34 1371 34 1681 34 3678
Fragments arranged by size:
3678 1681 1371 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4724 5934 4724
Size: 1210 5622
BclI T'GATC_A
Cuts at: 1548 2772 1548
Size: 1224 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 3578 5443
Size: 107 122 71 631 605 416 1490 1865
Cuts at: 5443 136
Size: 1525
Fragments arranged by size:
1865 1525 1490 631 605 416 122 107
71
BglII A'GATC_T
Cuts at: 3123 3753 6827 3123
Size: 630 3074 3128
Fragments arranged by size:
3128 3074 630
BlpI GC'TnA_GC
Cuts at: 3971 4249 3971
Size: 278 6554
Bme1580I G_kGCm'C
Cuts at: 1790 2423 3009 3019 3108 3200 4893 6049 1790
Size: 633 586 10 89 92 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 633 586 92 89 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6832
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 2410 3013
Size: 416 53 83 399 24 921 72 603
Cuts at: 3013 4563 5521 442
Size: 1550 958 1753
Fragments arranged by size:
1753 1550 958 921 603 416 399 83
72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6832
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 6832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 3089 3499 5492 1239
Size: 800 1050 410 1993 2579
Fragments arranged by size:
2579 1993 1050 800 410
Bpu10I CC'TnA_GC
Cuts at: 2402 3049 3804 2402
Size: 647 755 5430
Fragments arranged by size:
5430 755 647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 3946 4957 5822 6120 2309
Size: 1637 1011 865 298 3021
Fragments arranged by size:
3021 1637 1011 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3179 5495 882
Size: 617 1680 2316 2219
Fragments arranged by size:
2316 2219 1680 617
BsaAI yAC'GTr
Cuts at: 493 1204 4623 493
Size: 711 3419 2702
Fragments arranged by size:
3419 2702 711
BsaBI GATnn'nnATC
Cuts at: 6826 6826
Size: 6832
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2164 4639 5021
Size: 53 83 186 936 498 133 2475 382
Cuts at: 5021 5656 275
Size: 635 1451
Fragments arranged by size:
2475 1451 936 635 498 382 186 133
83 53
BsaWI w'CCGG_w
Cuts at: 5265 5663 5937 6084 6495 5265
Size: 398 274 147 411 5602
Fragments arranged by size:
5602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 5374
Fragments arranged by size:
5374 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 2819 3245 3410 3479 3497 3502 3800
Size: 1436 197 426 165 69 18 5 298
Cuts at: 3800 4063 4066 4069 1186
Size: 263 3 3 3949
Fragments arranged by size:
3949 1436 426 298 263 197 165 69
18 5 3 3
BseYI C'CCAG_C
Cuts at: 2138 4097 6035 6531 2138
Size: 1959 1938 496 2439
Fragments arranged by size:
2439 1959 1938 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 1163
Size: 573 921 5338
Fragments arranged by size:
5338 921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4282 5043 6071 1540
Size: 275 186 2281 761 1028 2301
Fragments arranged by size:
2301 2281 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2423 3108 4019 4893 4978 6049 2423
Size: 685 911 874 85 1071 3206
Fragments arranged by size:
3206 1071 911 874 685 85
BsmI GAATG_Cn'
Cuts at: 3406 4343 4436 3406
Size: 937 93 5802
Fragments arranged by size:
5802 937 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2423 3009 3019 3108 3200 3944 4019
Size: 399 633 586 10 89 92 744 75
Cuts at: 4019 4151 4893 4978 6049 1391
Size: 132 742 85 1071 2174
Fragments arranged by size:
2174 1071 744 742 633 586 399 132
92 89 85 75 10
BspEI T'CCGG_A
Cuts at: 6495 6495
Size: 6832
BspHI T'CATG_A
Cuts at: 2955 4721 6451 2955
Size: 1766 1730 3336
Fragments arranged by size:
3336 1766 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 4187 844
Size: 1841 1502 3489
Fragments arranged by size:
3489 1841 1502
BsrBI CCG'CTC
Cuts at: 1394 1823 3073 4719 1394
Size: 429 1250 1646 3507
Fragments arranged by size:
3507 1646 1250 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 5327 5501 65
Size: 1183 1165 2914 174 1396
Fragments arranged by size:
2914 1396 1183 1165 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5476 5663 1700
Size: 110 3666 187 2869
Fragments arranged by size:
3666 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6832
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6832
BssSI C'ACGA_G
Cuts at: 2634 3144 4892 5904 2634
Size: 510 1748 1012 3562
Fragments arranged by size:
3562 1748 1012 510
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6832
BstBI TT'CG_AA
Cuts at: 5636 5636
Size: 6832
BstEII G'GTnAC_C
Cuts at: 1184 2375 1184
Size: 1191 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2522 2522
Size: 6832
BstYI r'GATC_y
Cuts at: 1972 2655 2703 2782 2927 3123 3387 3730 3753
Size: 683 48 79 145 196 264 343 23
Cuts at: 3753 4121 4162 4529 4914 4931 6383 6469 6481
Size: 368 41 367 385 17 1452 86 12
Cuts at: 6481 6827 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 683 385 368 367 346 343
264 196 145 86 79 48 41 23
17 12
BstZ17I GTA'TAC
Cuts at: 3207 3207
Size: 6832
BtgI C'CryG_G
Cuts at: 513 1065 2004 2182 2586 513
Size: 552 939 178 404 4759
Fragments arranged by size:
4759 939 552 404 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3432 487
Size: 683 56 2206 3887
Fragments arranged by size:
3887 2206 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 4348 5142 5162 2627
Size: 1721 794 20 4297
Fragments arranged by size:
4297 1721 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6832
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6797
DraI TTT'AAA
Cuts at: 4136 4136
Size: 6832
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2384 5839 809
Size: 507 1068 3455 1802
Fragments arranged by size:
3455 1802 1068 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 3579 4279
Size: 54 1425 50 275 18 379 1370 700
Cuts at: 4279 5168 6797 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1370 889 700 379 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4279 1537
Size: 275 2467 4090
Fragments arranged by size:
4090 2467 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3456 4024 4040 4762 1353
Size: 1484 619 568 16 722 3423
Fragments arranged by size:
3423 1484 722 619 568 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 5419 5793 5939 387
Size: 1064 138 996 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 996 374 146 138
EcoICRI GAG'CTC
Cuts at: 4017 4017
Size: 6832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 3089 3196 3499 4089 4895 5492
Size: 393 407 1050 107 303 590 806 597
Cuts at: 5492 6279 6547 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1050 806 787 597 590 407 393
303 268 107
EcoO109I rG'GnC_Cy
Cuts at: 2305 3015 2305
Size: 710 6122
EcoRI G'AATT_C
Cuts at: 2450 4141 4519 2450
Size: 1691 378 4763
Fragments arranged by size:
4763 1691 378
FspI TGC'GCA
Cuts at: 5338 5338
Size: 6832
HaeII r_GCGC'y
Cuts at: 1678 2167 2758 2896 5979 1678
Size: 489 591 138 3083 2531
Fragments arranged by size:
3083 2531 591 489 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2583 2615 3110 3142 3681
Size: 32 135 32 1011 32 495 32 539
Cuts at: 3681 3713 3986 4001 4018 4033 4100 4132 5473
Size: 32 273 15 17 15 67 32 1341
Cuts at: 5473 5505 5547 5579 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 1011 539 495 273 135 67
42 32 32 32 32 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2069 2148 2859 3136 3259 4176
Size: 988 281 131 79 711 277 123 917
Cuts at: 4176 4422 669
Size: 246 3079
Fragments arranged by size:
3079 988 917 711 281 277 246 131
123 79
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6832
HpaI GTT'AAC
Cuts at: 2148 4422 2148
Size: 2274 4558
KasI G'GCGC_C
Cuts at: 2163 2163
Size: 6832
KpnI G_GTAC'C
Cuts at: 3153 4157 3153
Size: 1004 5828
MfeI C'AATT_G
Cuts at: 4431 4431
Size: 6832
MluI A'CGCG_T
Cuts at: 6708 6708
Size: 6832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5946 6130 1305
Size: 4641 184 2007
Fragments arranged by size:
4641 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2211 3581 10
Size: 54 1425 343 379 1370 3261
Fragments arranged by size:
3261 1425 1370 379 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2679 4790 5149 5308 518
Size: 648 1323 190 2111 359 159 2042
Fragments arranged by size:
2111 2042 1323 648 359 190 159
MspA1I CmG'CkG
Cuts at: 1606 2356 3067 4248 4925 6073 6318 1606
Size: 750 711 1181 677 1148 245 2120
Fragments arranged by size:
2120 1181 1148 750 711 677 245
NarI GG'CG_CC
Cuts at: 2164 2164
Size: 6832
NcoI C'CATG_G
Cuts at: 513 1065 2004 2182 513
Size: 552 939 178 5163
Fragments arranged by size:
5163 939 552 178
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6832
NotI GC'GGCC_GC
Cuts at: 4279 4279
Size: 6832
NspI r_CATG'y
Cuts at: 1132 1229 2923 3670 4190 5735 1132
Size: 97 1694 747 520 1545 2229
Fragments arranged by size:
2229 1694 1545 747 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6832
PciI A'CATG_T
Cuts at: 2919 5731 2919
Size: 2812 4020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6832
PfoI T'CCnGG_A
Cuts at: 2610 3390 2610
Size: 780 6052
PmeI GTTT'AAAC
Cuts at: 4136 4136
Size: 6832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4901 4933 6414 6446 4901
Size: 32 1481 32 5287
Fragments arranged by size:
5287 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 6832
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6832
PsiI TTA'TAA
Cuts at: 4402 4402
Size: 6832
PspOMI G'GGCC_C
Cuts at: 3015 3015
Size: 6832
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 6800
PstI C_TGCA'G
Cuts at: 830 2622 4184 830
Size: 1792 1562 3478
Fragments arranged by size:
3478 1792 1562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6832
PvuII CAG'CTG
Cuts at: 2356 2356
Size: 6832
SacI G_AGCT'C
Cuts at: 4019 4019
Size: 6832
SalI G'TCGA_C
Cuts at: 1936 2067 4174 1936
Size: 131 2107 4594
Fragments arranged by size:
4594 2107 131
SapI GCTCTTCn'nnn_
Cuts at: 4024 4024
Size: 6832
SbfI CC_TGCA'GG
Cuts at: 4184 4184
Size: 6832
ScaI AGT'ACT
Cuts at: 2721 4090 5080 6792 2721
Size: 1369 990 1712 2761
Fragments arranged by size:
2761 1712 1369 990
SfcI C'TryA_G
Cuts at: 826 1045 2618 4180 5315 5996 6187 826
Size: 219 1573 1562 1135 681 191 1471
Fragments arranged by size:
1573 1562 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3578 3578
Size: 6832
SfoI GGC'GCC
Cuts at: 2165 2165
Size: 6832
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6832
SmaI CCC'GGG
Cuts at: 1872 4159 1872
Size: 2287 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 3961 4936 5837 6099 820
Size: 197 926 345 1673 975 901 262 1553
Fragments arranged by size:
1673 1553 975 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 4623 493
Size: 4130 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6832
SphI G_CATG'C
Cuts at: 1132 4190 1132
Size: 3058 3774
SspI AAT'ATT
Cuts at: 5 52 4756 5
Size: 47 4704 2081
Fragments arranged by size:
4704 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 5806
StyI C'CwwG_G
Cuts at: 513 1065 2004 2182 2838 3001 3582 4271 513
Size: 552 939 178 656 163 581 689 3074
Fragments arranged by size:
3074 939 689 656 581 552 178 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2524 2528 4859 4876 5029 5214 5659
Size: 1255 944 4 2331 17 153 185 445
Cuts at: 5659 325
Size: 1498
Fragments arranged by size:
2331 1498 1255 944 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 4088 5078
Size: 274 80 33 51 1443 742 1369 990
Cuts at: 5078 6790 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1369 990 742 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 2606 2638 6792 6824 2436
Size: 32 138 32 4154 32 2444
Fragments arranged by size:
4154 2444 138 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6832
XbaI T'CTAG_A
Cuts at: 4168 4168
Size: 6832
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6832
XmaI C'CCGG_G
Cuts at: 1870 4157 1870
Size: 2287 4545
XmnI GAAnn'nnTTC
Cuts at: 4961 4961
Size: 6832
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2032 4640 276
Size: 53 83 186 936 498 2608 2468
Fragments arranged by size:
2608 2468 936 498 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI RsrII SacII SanDI
SexAI SrfI SwaI XcmI