Restriction map for
pFN21AB9980
(Circular) (Six-base) MAPSORT of: pFN21AB9980.seq Check: 5647 from: 1 to: 6829
pFN21AB9980 6829 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4172 1937
Size: 2235 4594
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3252 4892 6276 6544 1632
Size: 1620 1640 1384 268 1917
Fragments arranged by size:
1917 1640 1620 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 2851 5728 6705 1203
Size: 1648 2877 977 1327
Fragments arranged by size:
2877 1648 1327 977
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 5558 5558
Size: 6829
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6829
AlwNI CAG_nnn'CTG
Cuts at: 3794 4572 6144 3794
Size: 778 1572 4479
Fragments arranged by size:
4479 1572 778
ApaLI G'TGCA_C
Cuts at: 4886 6042 4886
Size: 1156 5673
ApoI r'AATT_y
Cuts at: 1493 2130 2280 2898 3155 3383 3751 4138 4364
Size: 637 150 618 257 228 368 387 226
Cuts at: 4364 4516 6625 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 637 618 387 368 257 228
226 152 150
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 4154 1870
Size: 73 288 1923 4545
Fragments arranged by size:
4545 1923 288 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2100 2133 2100
Size: 33 6796
BamHI G'GATC_C
Cuts at: 2549 4159 4526 2549
Size: 1610 367 4852
Fragments arranged by size:
4852 1610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4150 5605 618
Size: 325 217 627 2363 1455 1842
Fragments arranged by size:
2363 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4148 1391
Size: 2757 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3492 3969 928
Size: 971 1593 477 3788
Fragments arranged by size:
3788 1593 971 477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5020 5054 1903
Size: 34 3083 34 3678
Fragments arranged by size:
3678 3083 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 2290 1548
Size: 742 6087
BfrBI ATG'CAT
Cuts at: 2893 2893
Size: 6829
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5440 136
Size: 107 122 71 631 605 3768 1525
Fragments arranged by size:
3768 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3295 3593 6824 3295
Size: 298 3231 3300
Fragments arranged by size:
3300 3231 298
BlpI GC'TnA_GC
Cuts at: 4246 4246
Size: 6829
Bme1580I G_kGCm'C
Cuts at: 1790 4890 6046 1790
Size: 3100 1156 2573
Fragments arranged by size:
3100 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4560 5518 442
Size: 416 53 83 399 24 3143 958 1753
Fragments arranged by size:
3143 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3821 5489 1239
Size: 2582 1668 2579
Fragments arranged by size:
2582 2579 1668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 4954 5819 6117 2191
Size: 2763 865 298 2903
Fragments arranged by size:
2903 2763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2602 3777 5492 882
Size: 617 1103 1175 1715 2219
Fragments arranged by size:
2219 1715 1175 1103 617
BsaAI yAC'GTr
Cuts at: 493 1204 4620 493
Size: 711 3416 2702
Fragments arranged by size:
3416 2702 711
BsaBI GATnn'nnATC
Cuts at: 2067 4086 6823 2067
Size: 2019 2737 2073
Fragments arranged by size:
2737 2073 2019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4636 5018 5653 275
Size: 53 83 186 936 3103 382 635 1451
Fragments arranged by size:
3103 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2500 5262 5660 5934 6081 6492 2500
Size: 2762 398 274 147 411 2837
Fragments arranged by size:
2837 2762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2826 2856 1178
Size: 30 165 30 1423 30 5151
Fragments arranged by size:
5151 1423 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3413 3515 3620 3780 1186
Size: 2227 102 105 160 4235
Fragments arranged by size:
4235 2227 160 105 102
BseYI C'CCAG_C
Cuts at: 6032 6528 6032
Size: 496 6333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3356 1163
Size: 573 1620 4636
Fragments arranged by size:
4636 1620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4279 5040 6068 1540
Size: 275 186 2278 761 1028 2301
Fragments arranged by size:
2301 2278 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3589 4890 4975 6046 3589
Size: 1301 85 1071 4372
Fragments arranged by size:
4372 1301 1071 85
BsmI GAATG_Cn'
Cuts at: 4340 4433 4340
Size: 93 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3589 4148 4890 4975 6046 1391
Size: 399 1799 559 742 85 1071 2174
Fragments arranged by size:
2174 1799 1071 742 559 399 85
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 2191 2389 4718 6448 2191
Size: 198 2329 1730 2572
Fragments arranged by size:
2572 2329 1730 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 4184 844
Size: 1390 1950 3489
Fragments arranged by size:
3489 1950 1390
BsrBI CCG'CTC
Cuts at: 1394 1823 4716 1394
Size: 429 2893 3507
Fragments arranged by size:
3507 2893 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5324 5498 65
Size: 1183 4076 174 1396
Fragments arranged by size:
4076 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5473 5660 1700
Size: 110 3663 187 2869
Fragments arranged by size:
3663 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 4889 5901 4889
Size: 1012 5817
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2420 794
Size: 1626 5203
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 2549 3295 3593 4159 4526 4911 4928 6380
Size: 577 746 298 566 367 385 17 1452
Cuts at: 6380 6466 6478 6824 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 746 577 566 385 367 346
298 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2324 513
Size: 552 1259 5018
Fragments arranged by size:
5018 1259 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 3344 4345 5139 5159 3344
Size: 1001 794 20 5014
Fragments arranged by size:
5014 1001 794 20
ClaI AT'CG_AT
Cuts at: 1554 3495 1554
Size: 1941 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2460 2495 529
Size: 35 1896 35 4863
Fragments arranged by size:
4863 1896 35 35
DraI TTT'AAA
Cuts at: 2044 3877 4133 2044
Size: 1833 256 4740
Fragments arranged by size:
4740 1833 256
DraIII CAC_nnn'GTG
Cuts at: 2420 2914 2420
Size: 494 6335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3838 5836 809
Size: 507 2522 1998 1802
Fragments arranged by size:
2522 1998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4276 5165 6794
Size: 54 1425 50 275 18 2446 889 1629
Cuts at: 6794 8
Size: 43
Fragments arranged by size:
2446 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4276 1537
Size: 275 2464 4090
Fragments arranged by size:
4090 2464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2910 3228 3706 3718 3727 3736 3778 4120
Size: 1557 318 478 12 9 9 42 342
Cuts at: 4120 4759 1353
Size: 639 3423
Fragments arranged by size:
3423 1557 639 478 342 318 42 12
9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5416 5790 5936 387
Size: 1064 138 3827 374 146 1280
Fragments arranged by size:
3827 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3252 3821 4892 5489 6276 6544 1239
Size: 393 1620 569 1071 597 787 268 1524
Fragments arranged by size:
1620 1524 1071 787 597 569 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3270 3270
Size: 6829
EcoRI G'AATT_C
Cuts at: 3751 4138 4516 3751
Size: 387 378 6064
Fragments arranged by size:
6064 387 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 6829
FspI TGC'GCA
Cuts at: 5335 5335
Size: 6829
HaeII r_GCGC'y
Cuts at: 1678 5976 1678
Size: 4298 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 3222 3254 3674
Size: 32 135 32 1040 32 578 32 420
Cuts at: 3674 3706 4049 4081 5470 5502 5544 5576 1373
Size: 32 343 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1389 1040 578 420 343 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 4173 4419 669
Size: 988 281 364 1871 246 3079
Fragments arranged by size:
3079 1871 988 364 281 246
HindIII A'AGCT_T
Cuts at: 748 3004 748
Size: 2256 4573
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2786 3417 5943 6127 1305
Size: 1481 631 2526 184 2007
Fragments arranged by size:
2526 2007 1481 631 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5007
Fragments arranged by size:
5007 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2742 3474 4787 5146 5305 518
Size: 648 1576 732 1313 359 159 2042
Fragments arranged by size:
2042 1576 1313 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2782 4245 4922 6070 6315 1606
Size: 1176 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1176 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2324 513
Size: 552 1259 5018
Fragments arranged by size:
5018 1259 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NsiI A_TGCA'T
Cuts at: 2895 2895
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 2855 4187 5732 1132
Size: 97 1626 1332 1545 2229
Fragments arranged by size:
2229 1626 1545 1332 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6829
PciI A'CATG_T
Cuts at: 2851 5728 2851
Size: 2877 3952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6829
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2496 3795 1613
Size: 883 1299 4647
Fragments arranged by size:
4647 1299 883
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PspXI vC'TCGA_Gb
Cuts at: 2231 2231
Size: 6829
PstI C_TGCA'G
Cuts at: 830 2017 4181 830
Size: 1187 2164 3478
Fragments arranged by size:
3478 2164 1187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2782 2782
Size: 6829
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SapI GCTCTTCn'nnn_
Cuts at: 3778 3778
Size: 6829
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 2011 2680 3311 5077 6789 2011
Size: 669 631 1766 1712 2051
Fragments arranged by size:
2051 1766 1712 669 631
SfcI C'TryA_G
Cuts at: 826 1045 2013 3109 4177 5312 5993 6184 826
Size: 219 968 1096 1068 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 1068 968 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2206 2231 4933 5834 6096 820
Size: 197 926 263 25 2702 901 262 1553
Fragments arranged by size:
2702 1553 926 901 263 262 197 25
SnaBI TAC'GTA
Cuts at: 493 4620 493
Size: 4127 2702
SpeI A'CTAG_T
Cuts at: 152 4103 152
Size: 3951 2878
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 2049 2249 4753 5
Size: 47 1997 200 2504 2081
Fragments arranged by size:
2504 2081 1997 200 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6829
StyI C'CwwG_G
Cuts at: 513 1065 2324 2424 4268 513
Size: 552 1259 100 1844 3074
Fragments arranged by size:
3074 1844 1259 552 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2317 3356 4856 4873 5026 5211
Size: 1255 666 71 1039 1500 17 153 185
Cuts at: 5211 5656 325
Size: 445 1498
Fragments arranged by size:
1500 1498 1255 1039 666 445 185 153
71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2009 2678 3309
Size: 274 80 33 51 1443 32 669 631
Cuts at: 3309 3376 5075 6787 96
Size: 67 1699 1712 138
Fragments arranged by size:
1712 1699 1443 669 631 274 138 80
67 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2796 2828 6789 6821 2796
Size: 32 3961 32 2804
Fragments arranged by size:
3961 2804 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 3848 4165 3848
Size: 317 6512
XhoI C'TCGA_G
Cuts at: 1943 2231 1943
Size: 288 6541
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 4958 4958
Size: 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI