Restriction map for
pFN21AB9979
(Circular) (Six-base) MAPSORT of: pFN21AB9979.seq Check: 3958 from: 1 to: 6379
pFN21AB9979 6379 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3128 4442 5826 6094 1632
Size: 1079 417 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 1079 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3017 1184
Size: 1833 4546
AlwNI CAG_nnn'CTG
Cuts at: 3106 3403 4122 5694 3106
Size: 297 719 1572 3791
Fragments arranged by size:
3791 1572 719 297
ApaI G_GGCC'C
Cuts at: 2929 2929
Size: 6379
ApaLI G'TGCA_C
Cuts at: 2904 4436 5592 2904
Size: 1532 1156 3691
Fragments arranged by size:
3691 1532 1156
ApoI r'AATT_y
Cuts at: 1493 2268 2314 3688 3914 4066 6175 1493
Size: 775 46 1374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 775 226 152 46
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2534 3084 3645 3704 1870
Size: 73 591 550 561 59 4545
Fragments arranged by size:
4545 591 561 550 73 59
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2739 3468 3700 5155 618
Size: 325 217 627 952 729 232 1455 1842
Fragments arranged by size:
1842 1455 952 729 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2929 3448 3499 3654 3698 1391
Size: 1538 519 51 155 44 4072
Fragments arranged by size:
4072 1538 519 155 51 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 3150 928
Size: 971 203 1048 4157
Fragments arranged by size:
4157 1048 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2337 4271 5481 2337
Size: 1934 1210 3235
Fragments arranged by size:
3235 1934 1210
BclI T'GATC_A
Cuts at: 1548 3462 1548
Size: 1914 4465
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4990 136
Size: 107 122 71 631 605 3318 1525
Fragments arranged by size:
3318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2023 6374 2023
Size: 4351 2028
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 2908 2929 3428 4440 5596 1790
Size: 1118 21 499 1012 1156 2573
Fragments arranged by size:
2573 1156 1118 1012 499 21
BmgBI CAC'GTC
Cuts at: 1318 2630 1318
Size: 1312 5067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4110 5068 442
Size: 416 53 83 399 24 2693 958 1753
Fragments arranged by size:
2693 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 2776 3002 3083 3522 5039 1239
Size: 1394 143 226 81 439 1517 2579
Fragments arranged by size:
2579 1517 1394 439 226 143 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1995 2394 2835 2999 3057 3657 4504 5369 5667
Size: 399 441 164 58 600 847 865 298
Cuts at: 5667 1995
Size: 2707
Fragments arranged by size:
2707 865 847 600 441 399 298 164
58
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 4170 493
Size: 711 912 2054 2702
Fragments arranged by size:
2702 2054 912 711
BsaBI GATnn'nnATC
Cuts at: 2439 6373 2439
Size: 3934 2445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2546 4186 4568 5203
Size: 53 83 186 936 1013 1640 382 635
Cuts at: 5203 275
Size: 1451
Fragments arranged by size:
1640 1451 1013 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3081 3111 1178
Size: 30 165 30 1678 30 4446
Fragments arranged by size:
4446 1678 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 3467 1186
Size: 1417 864 4098
Fragments arranged by size:
4098 1417 864
BseYI C'CCAG_C
Cuts at: 2094 2747 3352 5582 6078 2094
Size: 653 605 2230 496 2395
Fragments arranged by size:
2395 2230 653 605 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3435 1163
Size: 573 1699 4107
Fragments arranged by size:
4107 1699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3516 3829 4590 5618 1540
Size: 275 186 1515 313 761 1028 2301
Fragments arranged by size:
2301 1515 1028 761 313 275 186
BsiHKAI G_wGCw'C
Cuts at: 2908 3325 3448 3654 4440 4525 5596 2908
Size: 417 123 206 786 85 1071 3691
Fragments arranged by size:
3691 1071 786 417 206 123 85
BsmI GAATG_Cn'
Cuts at: 2828 3890 3983 2828
Size: 1062 93 5224
Fragments arranged by size:
5224 1062 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2908 2929 3325 3428 3448 3499 3654
Size: 399 1118 21 396 103 20 51 155
Cuts at: 3654 3698 4440 4525 5596 1391
Size: 44 742 85 1071 2174
Fragments arranged by size:
2174 1118 1071 742 399 396 155 103
85 51 44 21 20
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3411 3734 844
Size: 2567 323 3489
Fragments arranged by size:
3489 2567 323
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2769 3375 4874 5048 65
Size: 1183 1521 606 1499 174 1396
Fragments arranged by size:
1521 1499 1396 1183 606 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3594 96
Size: 3498 2881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 2556 3563 4439 5451 2556
Size: 1007 876 1012 3484
Fragments arranged by size:
3484 1012 1007 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 3155 3155
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2023 3709 4076 4461 4478 5930 6016 6028
Size: 51 1686 367 385 17 1452 86 12
Cuts at: 6028 6374 1972
Size: 346 1977
Fragments arranged by size:
1977 1686 1452 385 367 346 86 51
17 12
Bsu36I CC'TnA_GG
Cuts at: 2138 2637 2138
Size: 499 5880
BtgI C'CryG_G
Cuts at: 513 1065 2004 3051 3261 513
Size: 552 939 1047 210 3631
Fragments arranged by size:
3631 1047 939 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2826 487
Size: 683 56 1600 4040
Fragments arranged by size:
4040 1600 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 3546 3895 4689 4709 2969
Size: 577 349 794 20 4639
Fragments arranged by size:
4639 794 577 349 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3157 5386 809
Size: 507 1841 2229 1802
Fragments arranged by size:
2229 1841 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3826 4715 6344
Size: 54 1425 50 275 18 1996 889 1629
Cuts at: 6344 8
Size: 43
Fragments arranged by size:
1996 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4309 1353
Size: 2956 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3446 3652 3446
Size: 206 6173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2633 2711 2776 3002 3083 3128 3522
Size: 393 1001 78 65 226 81 45 394
Cuts at: 3522 4442 5039 5826 6094 1239
Size: 920 597 787 268 1524
Fragments arranged by size:
1524 1001 920 787 597 394 393 268
226 81 78 65 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2603 2603
Size: 6379
EcoO109I rG'GnC_Cy
Cuts at: 2925 2926 3159 3170 3400 2925
Size: 1 233 11 230 5904
Fragments arranged by size:
5904 233 230 11 1
EcoRI G'AATT_C
Cuts at: 2314 3688 4066 2314
Size: 1374 378 4627
Fragments arranged by size:
4627 1374 378
EcoRV GAT'ATC
Cuts at: 3079 3079
Size: 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 3040 2417
Size: 623 5756
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 2791 2823 3081
Size: 32 135 32 978 32 209 32 258
Cuts at: 3081 3113 5020 5052 5094 5126 1373
Size: 32 1907 32 42 32 2626
Fragments arranged by size:
2626 1907 978 258 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2210 2593 3723 3969 669
Size: 988 281 272 383 1130 246 3079
Fragments arranged by size:
3079 1130 988 383 281 272 246
HindIII A'AGCT_T
Cuts at: 748 2057 2223 748
Size: 1309 166 4904
Fragments arranged by size:
4904 1309 166
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 2202 3978 2202
Size: 1776 4603
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2210 5493 5677 1305
Size: 905 3283 184 2007
Fragments arranged by size:
3283 2007 905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4557
Fragments arranged by size:
4557 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2502 2628 3536 4337 4696 4855 518
Size: 648 1336 126 908 801 359 159 2042
Fragments arranged by size:
2042 1336 908 801 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2717 3001 3460 3795 4472 5620 5865 1606
Size: 1111 284 459 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 1111 677 459 335 284 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3051 513
Size: 552 939 1047 3841
Fragments arranged by size:
3841 1047 939 552
NdeI CA'TA_TG
Cuts at: 387 2251 387
Size: 1864 4515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 3737 5282 1132
Size: 97 2508 1545 2229
Fragments arranged by size:
2508 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3364 1108
Size: 2256 4123
PfoI T'CCnGG_A
Cuts at: 2127 2339 2910 3499 2127
Size: 212 571 589 5007
Fragments arranged by size:
5007 589 571 212
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PmlI CAC'GTG
Cuts at: 2116 2116
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 4448 4480 5961 5993 3170
Size: 32 1246 32 1481 32 3556
Fragments arranged by size:
3556 1481 1246 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3159 3170 3400 3159
Size: 11 230 6138
Fragments arranged by size:
6138 230 11
PshAI GACnn'nnGTC
Cuts at: 1613 2106 3332 1613
Size: 493 1226 4660
Fragments arranged by size:
4660 1226 493
PsiI TTA'TAA
Cuts at: 2831 3949 2831
Size: 1118 5261
PspOMI G'GGCC_C
Cuts at: 2925 2925
Size: 6379
PstI C_TGCA'G
Cuts at: 830 3408 3731 830
Size: 2578 323 3478
Fragments arranged by size:
3478 2578 323
PvuI CG_AT'CG
Cuts at: 2001 3516 2001
Size: 1515 4864
PvuII CAG'CTG
Cuts at: 2717 3001 3460 2717
Size: 284 459 5636
Fragments arranged by size:
5636 459 284
SacI G_AGCT'C
Cuts at: 3448 3654 3448
Size: 206 6173
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2430 2718 3404 3727 4862 5543 5734
Size: 219 1385 288 686 323 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1471 1385 1135 686 681 323 288 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 2409 2850 3014 3072 3672
Size: 197 926 67 399 441 164 58 600
Cuts at: 3672 4483 5384 5646 820
Size: 811 901 262 1553
Fragments arranged by size:
1553 926 901 811 600 441 399 262
197 164 67 58
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 3051 3818 513
Size: 552 939 1047 767 3074
Fragments arranged by size:
3074 1047 939 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3594 4625 6337
Size: 274 80 33 51 1443 1617 1031 1712
Cuts at: 6337 96
Size: 138
Fragments arranged by size:
1712 1617 1443 1031 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2335 2367 6339 6371 2335
Size: 32 3972 32 2343
Fragments arranged by size:
3972 2343 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI