Restriction map for
pFN21AB9976
(Circular) (Six-base) MAPSORT of: pFN21AB9976.seq Check: 2709 from: 1 to: 6826
pFN21AB9976 6826 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3082 4169 1937
Size: 1145 1087 4594
Fragments arranged by size:
4594 1145 1087
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4889 6273 6541 1632
Size: 3257 1384 268 1917
Fragments arranged by size:
3257 1917 1384 268
AfeI AGC'GCT
Cuts at: 4001 4001
Size: 6826
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 2058 1166
Size: 892 5934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4032 1184
Size: 2848 3978
AlwNI CAG_nnn'CTG
Cuts at: 4569 6141 4569
Size: 1572 5254
ApaI G_GGCC'C
Cuts at: 2428 2585 2609 2781 2796 2428
Size: 157 24 172 15 6458
Fragments arranged by size:
6458 172 157 24 15
ApaLI G'TGCA_C
Cuts at: 4883 6039 4883
Size: 1156 5670
ApoI r'AATT_y
Cuts at: 1493 2939 3254 3893 4135 4361 4513 6622 1493
Size: 1446 315 639 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 639 315 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2155 2419 2489 2578 2584 2668
Size: 73 67 145 264 70 89 6 84
Cuts at: 2668 4151 1870
Size: 1483 4545
Fragments arranged by size:
4545 1483 264 145 89 84 73 70
67 6
BamHI G'GATC_C
Cuts at: 3814 4156 4523 3814
Size: 342 367 6117
Fragments arranged by size:
6117 367 342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 3774 4147 5602 618
Size: 325 217 627 707 1280 373 1455 1842
Fragments arranged by size:
1842 1455 1280 707 627 373 325 217
BanII G_rGCy'C
Cuts at: 1391 2428 2585 2609 2727 2781 2796 4145 1391
Size: 1037 157 24 118 54 15 1349 4072
Fragments arranged by size:
4072 1349 1037 157 118 54 24 15
BbeI G_GCGC'C
Cuts at: 2498 3778 2498
Size: 1280 5546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3371 928
Size: 971 1472 4383
Fragments arranged by size:
4383 1472 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2460 2494 2986 3020 5017 5051 1903
Size: 34 523 34 492 34 1997 34 3678
Fragments arranged by size:
3678 1997 523 492 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4718 5928 4718
Size: 1210 5616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 2166 2647
Size: 107 122 71 631 605 410 84 481
Cuts at: 2647 2791 3804 5437 136
Size: 144 1013 1633 1525
Fragments arranged by size:
1633 1525 1013 631 605 481 410 144
122 107 84 71
BglII A'GATC_T
Cuts at: 2817 3230 3616 6821 2817
Size: 413 386 3205 2822
Fragments arranged by size:
3205 2822 413 386
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 2428 2585 2609 2781 2796 4887 6043 1790
Size: 638 157 24 172 15 2091 1156 2573
Fragments arranged by size:
2573 2091 1156 638 172 157 24 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 6826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2371 2889 4050 5486 1239
Size: 1132 518 1161 1436 2579
Fragments arranged by size:
2579 1436 1161 1132 518
Bpu10I CC'TnA_GC
Cuts at: 2172 2340 2598 2172
Size: 168 258 6400
Fragments arranged by size:
6400 258 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2878 4951 5816 6114 2878
Size: 2073 865 298 3590
Fragments arranged by size:
3590 2073 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5489 882
Size: 617 3990 2219
Fragments arranged by size:
3990 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4617 493
Size: 711 3413 2702
Fragments arranged by size:
3413 2702 711
BsaBI GATnn'nnATC
Cuts at: 3582 6820 3582
Size: 3238 3588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2495 3775 4633 5015
Size: 53 83 186 936 962 1280 858 382
Cuts at: 5015 5650 275
Size: 635 1451
Fragments arranged by size:
1451 1280 962 936 858 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2022 5259 5657 5931 6078 6489 2022
Size: 3237 398 274 147 411 2359
Fragments arranged by size:
3237 2359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2551 3310 3340 1178
Size: 30 165 30 1118 30 759 30 4664
Fragments arranged by size:
4664 1118 759 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2079 2529 2825 2875 3318 3426 3722 3864
Size: 893 450 296 50 443 108 296 142
Cuts at: 3864 1186
Size: 4148
Fragments arranged by size:
4148 893 450 443 296 296 142 108
50
BseYI C'CCAG_C
Cuts at: 3447 6029 6525 3447
Size: 2582 496 3748
Fragments arranged by size:
3748 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6253
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 2803 4276 5037 6065 1540
Size: 275 186 76 726 1473 761 1028 2301
Fragments arranged by size:
2301 1473 1028 761 726 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 4887 4972 6043 4887
Size: 85 1071 5670
Fragments arranged by size:
5670 1071 85
BsmI GAATG_Cn'
Cuts at: 4337 4430 4337
Size: 93 6733
BsmBI CGTCTCn'nnnn_
Cuts at: 3619 3619
Size: 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2428 2585 2609 2727 2781 2796 4145
Size: 399 638 157 24 118 54 15 1349
Cuts at: 4145 4887 4972 6043 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1349 1071 742 638 399 157 118
85 54 24 15
BspEI T'CCGG_A
Cuts at: 2022 6489 2022
Size: 4467 2359
BspHI T'CATG_A
Cuts at: 3586 4715 6445 3586
Size: 1129 1730 3967
Fragments arranged by size:
3967 1730 1129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 4181 844
Size: 2082 1255 3489
Fragments arranged by size:
3489 2082 1255
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2357 4713 1394
Size: 429 343 191 2356 3507
Fragments arranged by size:
3507 2356 429 343 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 3861 5321 5495 65
Size: 1183 2613 1460 174 1396
Fragments arranged by size:
2613 1460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5470 5657 1700
Size: 110 3660 187 2869
Fragments arranged by size:
3660 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6826
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstXI CCAn_nnnn'nTGG
Cuts at: 2060 2060
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2817 3230 3616 3814 3868 4156 4523 4908
Size: 845 413 386 198 54 288 367 385
Cuts at: 4908 4925 6377 6463 6475 6821 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 845 413 386 385 367 346
288 198 86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2541 2541
Size: 6826
BtgI C'CryG_G
Cuts at: 513 1065 2077 2142 2568 2728 3818 513
Size: 552 1012 65 426 160 1090 3521
Fragments arranged by size:
3521 1090 1012 552 426 160 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2905 3496 487
Size: 683 56 1679 591 3817
Fragments arranged by size:
3817 1679 683 591 56
BtsI GCAGTG_nn'
Cuts at: 3304 4342 5136 5156 3304
Size: 1038 794 20 4974
Fragments arranged by size:
4974 1038 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 2839 4130 2839
Size: 1291 5535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5833 809
Size: 507 4517 1802
Fragments arranged by size:
4517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2689 2800
Size: 54 1425 50 275 18 244 615 111
Cuts at: 2800 4273 5162 6791 8
Size: 1473 889 1629 43
Fragments arranged by size:
1629 1473 1425 889 615 275 244 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2074 2800 4273 1537
Size: 275 262 726 1473 4090
Fragments arranged by size:
4090 1473 726 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 2326 3718 3726 4086 4756 1353
Size: 973 1392 8 360 670 3423
Fragments arranged by size:
3423 1392 973 670 360 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2094 2553 3861 5413 5787 5933
Size: 1064 138 505 459 1308 1552 374 146
Cuts at: 5933 387
Size: 1280
Fragments arranged by size:
1552 1308 1280 1064 505 459 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 2889 4050 4889 5486 6273 6541
Size: 393 739 518 1161 839 597 787 268
Cuts at: 6541 1239
Size: 1524
Fragments arranged by size:
1524 1161 839 787 739 597 518 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3906 3906
Size: 6826
EcoO109I rG'GnC_Cy
Cuts at: 2147 2345 2424 2425 2524 2594 2605 2777 2778
Size: 198 79 1 99 70 11 172 1
Cuts at: 2778 4022 4040 4055 2147
Size: 1244 18 15 4918
Fragments arranged by size:
4918 1244 198 172 99 79 70 18
15 11 1 1
EcoRI G'AATT_C
Cuts at: 2939 4135 4513 2939
Size: 1196 378 5252
Fragments arranged by size:
5252 1196 378
EcoRV GAT'ATC
Cuts at: 2926 2926
Size: 6826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3395 3395
Size: 6826
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 2498 3778 4003 5973 1678
Size: 820 1280 225 1970 2531
Fragments arranged by size:
2531 1970 1280 820 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3310 3313 3342 3345 3730
Size: 32 135 32 1738 3 29 3 385
Cuts at: 3730 3762 5467 5499 5541 5573 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1738 1705 385 135 42 32 32
32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3749 4170 4416 669
Size: 988 281 1811 421 246 3079
Fragments arranged by size:
3079 1811 988 421 281 246
HindIII A'AGCT_T
Cuts at: 748 3017 748
Size: 2269 4557
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KasI G'GCGC_C
Cuts at: 2494 3774 2494
Size: 1280 5546
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 3695 4425 3695
Size: 730 6096
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 3144 5940 6124 1305
Size: 1175 664 2796 184 2007
Fragments arranged by size:
2796 2007 1175 664 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5004
Fragments arranged by size:
5004 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2058 2890 3836 4784 5143 5302 518
Size: 648 892 832 946 948 359 159 2042
Fragments arranged by size:
2042 948 946 892 832 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2520 2570 2730 4242 4919 6067 6312
Size: 473 441 50 160 1512 677 1148 245
Cuts at: 6312 1606
Size: 2120
Fragments arranged by size:
2120 1512 1148 677 473 441 245 160
50
NarI GG'CG_CC
Cuts at: 2495 3775 2495
Size: 1280 5546
NcoI C'CATG_G
Cuts at: 513 1065 2142 513
Size: 552 1077 5197
Fragments arranged by size:
5197 1077 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 2074 2800 4273 2074
Size: 726 1473 4627
Fragments arranged by size:
4627 1473 726
NspI r_CATG'y
Cuts at: 1132 1229 3113 3835 3991 4184 5729 1132
Size: 97 1884 722 156 193 1545 2229
Fragments arranged by size:
2229 1884 1545 722 193 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6826
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6826
PfoI T'CCnGG_A
Cuts at: 2359 2359
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 6408 6440 4895
Size: 32 1481 32 5281
Fragments arranged by size:
5281 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2147 2345 2524 4040 2147
Size: 198 179 1516 4933
Fragments arranged by size:
4933 1516 198 179
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PspOMI G'GGCC_C
Cuts at: 2424 2581 2605 2777 2792 2424
Size: 157 24 172 15 6458
Fragments arranged by size:
6458 172 157 24 15
PstI C_TGCA'G
Cuts at: 830 2473 4178 830
Size: 1643 1705 3478
Fragments arranged by size:
3478 1705 1643
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
SacII CC_GC'GG
Cuts at: 2080 2571 2731 2080
Size: 491 160 6175
Fragments arranged by size:
6175 491 160
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SanDI GG'GwC_CC
Cuts at: 2345 2524 2345
Size: 179 6647
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SfcI C'TryA_G
Cuts at: 826 1045 2469 3234 4174 5309 5990 6181 826
Size: 219 1424 765 940 1135 681 191 1471
Fragments arranged by size:
1471 1424 1135 940 765 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2082 2166 2082
Size: 84 6742
SfoI GGC'GCC
Cuts at: 2496 3776 2496
Size: 1280 5546
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2157 2491 2580 2586 2670 4153 1872
Size: 285 334 89 6 84 1483 4545
Fragments arranged by size:
4545 1483 334 285 89 84 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2857 4930 5831 6093 820
Size: 197 926 914 2073 901 262 1553
Fragments arranged by size:
2073 1553 926 914 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SrfI GCCC'GGGC
Cuts at: 2157 2670 2157
Size: 513 6313
SspI AAT'ATT
Cuts at: 5 52 3473 4750 5
Size: 47 3421 1277 2081
Fragments arranged by size:
3421 2081 1277 47
StuI AGG'CCT
Cuts at: 1517 2866 1517
Size: 1349 5477
StyI C'CwwG_G
Cuts at: 513 1065 2142 4265 513
Size: 552 1077 2123 3074
Fragments arranged by size:
3074 2123 1077 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2518 3085 3830 4019 4853 4870 5023
Size: 1255 938 567 745 189 834 17 153
Cuts at: 5023 5208 5653 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 938 834 745 567 445 189
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5072 6784 96
Size: 274 80 33 51 1443 3095 1712 138
Fragments arranged by size:
3095 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6786 6818 6786
Size: 32 6794
Tth111I GACn'n_nGTC
Cuts at: 1316 4038 1316
Size: 2722 4104
XbaI T'CTAG_A
Cuts at: 3026 4162 3026
Size: 1136 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3160 3055
Size: 105 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 2155 2489 2578 2584 2668 4151 1870
Size: 285 334 89 6 84 1483 4545
Fragments arranged by size:
4545 1483 334 285 89 84 6
XmnI GAAnn'nnTTC
Cuts at: 3657 4955 3657
Size: 1298 5528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SapI SexAI SwaI