Restriction map for
pFN21AB9974
(Circular) (Six-base) MAPSORT of: pFN21AB9974.seq Check: 2745 from: 1 to: 6826
pFN21AB9974 6826 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3996 4636 278
Size: 53 83 186 936 2460 640 2468
Fragments arranged by size:
2468 2460 936 640 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4169 1937
Size: 2232 4594
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 4889 6273 6541 1632
Size: 665 2592 1384 268 1917
Fragments arranged by size:
2592 1917 1384 665 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 2252 2802 5657 2252
Size: 550 2855 3421
Fragments arranged by size:
3421 2855 550
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 2087 2171 2477 3521 1166
Size: 921 84 306 1044 4471
Fragments arranged by size:
4471 1044 921 306 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 6794
AlwNI CAG_nnn'CTG
Cuts at: 2023 4569 6141 2023
Size: 2546 1572 2708
Fragments arranged by size:
2708 2546 1572
ApaI G_GGCC'C
Cuts at: 3291 3662 3797 3853 3291
Size: 371 135 56 6264
Fragments arranged by size:
6264 371 135 56
ApaLI G'TGCA_C
Cuts at: 4883 6039 4883
Size: 1156 5670
ApoI r'AATT_y
Cuts at: 1493 4135 4361 4513 6622 1493
Size: 2642 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2246 2838 2997 3796 3968 4151
Size: 73 226 77 592 159 799 172 183
Cuts at: 4151 1870
Size: 4545
Fragments arranged by size:
4545 799 592 226 183 172 159 77
73
BamHI G'GATC_C
Cuts at: 2376 3931 4156 4523 2376
Size: 1555 225 367 4679
Fragments arranged by size:
4679 1555 367 225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2352 3111 3324 3756 3894
Size: 325 217 627 565 759 213 432 138
Cuts at: 3894 3973 4147 5602 618
Size: 79 174 1455 1842
Fragments arranged by size:
1842 1455 759 627 565 432 325 217
213 174 138 79
BanII G_rGCy'C
Cuts at: 1391 2565 3291 3662 3797 3853 4145 1391
Size: 1174 726 371 135 56 292 4072
Fragments arranged by size:
4072 1174 726 371 292 135 56
BbeI G_GCGC'C
Cuts at: 3115 3760 3898 3115
Size: 645 138 6043
Fragments arranged by size:
6043 645 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 3040 3988 928
Size: 971 501 640 948 3766
Fragments arranged by size:
3766 971 948 640 501
BbvCI CC'TCA_GC
Cuts at: 3472 3472
Size: 6826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2408 2442 2632 2666 5017 5051 1903
Size: 34 471 34 190 34 2351 34 3678
Fragments arranged by size:
3678 2351 471 190 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3173 4718 5928 2550
Size: 623 1545 1210 3448
Fragments arranged by size:
3448 1545 1210 623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3833 5437 136
Size: 107 122 71 631 605 2161 1604 1525
Fragments arranged by size:
2161 1604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6821 6821
Size: 6826
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 3291 3662 3797 3853 4118 4887 6043 1790
Size: 1501 371 135 56 265 769 1156 2573
Fragments arranged by size:
2573 1501 1156 769 371 265 135 56
BmgBI CAC'GTC
Cuts at: 1318 3310 3832 3913 1318
Size: 1992 522 81 4231
Fragments arranged by size:
4231 1992 522 81
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 3745 2737
Size: 1008 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2210 3922 5486 1239
Size: 882 89 1712 1564 2579
Fragments arranged by size:
2579 1712 1564 882 89
Bpu10I CC'TnA_GC
Cuts at: 3351 3472 3351
Size: 121 6705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 4951 5816 6114 2330
Size: 2621 865 298 3042
Fragments arranged by size:
3042 2621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2097 2243 5489 882
Size: 617 598 146 3246 2219
Fragments arranged by size:
3246 2219 617 598 146
BsaAI yAC'GTr
Cuts at: 493 1204 4093 4617 493
Size: 711 2889 524 2702
Fragments arranged by size:
2889 2702 711 524
BsaBI GATnn'nnATC
Cuts at: 6820 6820
Size: 6826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 2459 2607 2649
Size: 53 83 186 936 888 38 148 42
Cuts at: 2649 3112 3570 3757 3802 3895 3993 4633 5015
Size: 463 458 187 45 93 98 640 382
Cuts at: 5015 5650 275
Size: 635 1451
Fragments arranged by size:
1451 936 888 640 635 463 458 382
187 186 148 98 93 83 53 45
42 38
BsaWI w'CCGG_w
Cuts at: 2252 2802 3413 3535 3855 3957 5259 5657 5931
Size: 550 611 122 320 102 1302 398 274
Cuts at: 5931 6078 6489 2252
Size: 147 411 2589
Fragments arranged by size:
2589 1302 611 550 411 398 320 274
147 122 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 2909 2939 3816
Size: 30 165 30 1332 30 144 30 877
Cuts at: 3816 3846 1178
Size: 30 4158
Fragments arranged by size:
4158 1332 877 165 144 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3734 1186
Size: 2548 4278
BseYI C'CCAG_C
Cuts at: 2044 2819 3223 3790 3814 6029 6525 2044
Size: 775 404 567 24 2215 496 2345
Fragments arranged by size:
2345 2215 775 567 496 404 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2719 2791 2893 1163
Size: 573 983 72 102 5096
Fragments arranged by size:
5096 983 573 102 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 2803 2923 2968 3653 4276
Size: 275 186 748 54 120 45 685 623
Cuts at: 4276 5037 6065 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 685 623 275 186
120 54 45
BsiHKAI G_wGCw'C
Cuts at: 2037 2094 2529 4887 4972 6043 2037
Size: 57 435 2358 85 1071 2820
Fragments arranged by size:
2820 2358 1071 435 85 57
BsmI GAATG_Cn'
Cuts at: 2595 4337 4430 2595
Size: 1742 93 4991
Fragments arranged by size:
4991 1742 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2526 2598 2526
Size: 72 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2094 2529 2565 3291 3662 3797
Size: 399 247 57 435 36 726 371 135
Cuts at: 3797 3853 4118 4145 4887 4972 6043 1391
Size: 56 265 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 726 435 399 371 265
247 135 85 57 56 36 27
BspEI T'CCGG_A
Cuts at: 3855 6489 3855
Size: 2634 4192
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4181 844
Size: 3337 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2464 2721 3727 4713 1394
Size: 429 641 257 1006 986 3507
Fragments arranged by size:
3507 1006 986 641 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 2854 5321 5495 65
Size: 1183 1606 2467 174 1396
Fragments arranged by size:
2467 1606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 2802 3648 5470 5657 1700
Size: 110 442 550 846 1822 187 2869
Fragments arranged by size:
2869 1822 846 550 442 187 110
BsrGI T'GTAC_A
Cuts at: 96 2481 3771 96
Size: 2385 1290 3151
Fragments arranged by size:
3151 2385 1290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 3194 4886 5898 3194
Size: 1692 1012 4122
Fragments arranged by size:
4122 1692 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 3841 1184
Size: 2657 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 3952 3952
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2376 3931 4156 4523 4908 4925 6377 6463
Size: 404 1555 225 367 385 17 1452 86
Cuts at: 6463 6475 6821 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1555 1452 404 385 367 346 225
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4119 4119
Size: 6826
BtgI C'CryG_G
Cuts at: 513 1065 2294 2402 2778 2913 3100 3356 3387
Size: 552 1229 108 376 135 187 256 31
Cuts at: 3387 513
Size: 3952
Fragments arranged by size:
3952 1229 552 376 256 187 135 108
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3559 487
Size: 683 56 2333 3754
Fragments arranged by size:
3754 2333 683 56
BtsI GCAGTG_nn'
Cuts at: 3495 4118 4342 5136 5156 3495
Size: 623 224 794 20 5165
Fragments arranged by size:
5165 794 623 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5833 809
Size: 507 4517 1802
Fragments arranged by size:
4517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 3214 3513
Size: 54 1425 50 275 18 951 433 299
Cuts at: 3513 3650 4273 5162 6791 8
Size: 137 623 889 1629 43
Fragments arranged by size:
1629 1425 951 889 623 433 299 275
137 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3650 4273 1537
Size: 275 1838 623 4090
Fragments arranged by size:
4090 1838 623 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2984 4756 1353
Size: 746 885 1772 3423
Fragments arranged by size:
3423 1772 885 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2893 5413 5787 5933 387
Size: 1064 138 1304 2520 374 146 1280
Fragments arranged by size:
2520 1304 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2210 2297 3922 4889 5486 6273
Size: 393 489 89 87 1625 967 597 787
Cuts at: 6273 6541 1239
Size: 268 1524
Fragments arranged by size:
1625 1524 967 787 597 489 393 268
89 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 3068 2642
Size: 426 6400
EcoO109I rG'GnC_Cy
Cuts at: 2207 2305 3287 3288 3464 3488 3658 3659 3849
Size: 98 982 1 176 24 170 1 190
Cuts at: 3849 3850 2207
Size: 1 5183
Fragments arranged by size:
5183 982 190 176 170 98 24 1
1 1
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3178 3760 3178
Size: 582 6244
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 2077 2271 3115 3257 3445 3760 3898 3945
Size: 399 194 844 142 188 315 138 47
Cuts at: 3945 5973 1678
Size: 2028 2531
Fragments arranged by size:
2531 2028 844 399 315 194 188 142
138 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2183 2215 2618
Size: 32 135 32 529 32 50 32 403
Cuts at: 2618 2650 2735 2767 2909 2941 3224 3256 3916
Size: 32 85 32 142 32 283 32 660
Cuts at: 3916 3948 5467 5499 5541 5573 1373
Size: 32 1519 32 42 32 2626
Fragments arranged by size:
2626 1519 660 529 403 283 142 135
85 50 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4170 4416 669
Size: 988 281 2232 246 3079
Fragments arranged by size:
3079 2232 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3737 748
Size: 2989 3837
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KasI G'GCGC_C
Cuts at: 3111 3756 3894 3111
Size: 645 138 6043
Fragments arranged by size:
6043 645 138
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 4425 4425
Size: 6826
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 2778 2952 3389 5940 6124 1305
Size: 1379 94 174 437 2551 184 2007
Fragments arranged by size:
2551 2007 1379 437 184 174 94
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5004
Fragments arranged by size:
5004 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2171 2477 3330 3521 4784 5143
Size: 648 921 84 306 853 191 1263 359
Cuts at: 5143 5302 518
Size: 159 2042
Fragments arranged by size:
2042 1263 921 853 648 359 306 191
159 84
MspA1I CmG'CkG
Cuts at: 1606 2134 2404 2940 3102 3452 3476 3595 3888
Size: 528 270 536 162 350 24 119 293
Cuts at: 3888 4242 4919 6067 6312 1606
Size: 354 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 528 354 350 293
270 245 162 119 24
NaeI GCC'GGC
Cuts at: 3650 3650
Size: 6826
NarI GG'CG_CC
Cuts at: 3112 3757 3895 3112
Size: 645 138 6043
Fragments arranged by size:
6043 645 138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NgoMIV G'CCGG_C
Cuts at: 3648 3648
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 4184 5729 1132
Size: 97 2955 1545 2229
Fragments arranged by size:
2955 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2638 1348
Size: 1290 5536
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3223 1108
Size: 2115 4711
PfoI T'CCnGG_A
Cuts at: 4055 4055
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 6408 6440 4895
Size: 32 1481 32 5281
Fragments arranged by size:
5281 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 3464 3488 2305
Size: 1159 24 5643
Fragments arranged by size:
5643 1159 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PspOMI G'GGCC_C
Cuts at: 3287 3658 3793 3849 3287
Size: 371 135 56 6264
Fragments arranged by size:
6264 371 135 56
PstI C_TGCA'G
Cuts at: 830 4113 4178 830
Size: 3283 65 3478
Fragments arranged by size:
3478 3283 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2134 3452 3476 3595 2134
Size: 1318 24 119 5365
Fragments arranged by size:
5365 1318 119 24
SacII CC_GC'GG
Cuts at: 2405 3103 2405
Size: 698 6128
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SanDI GG'GwC_CC
Cuts at: 2305 2305
Size: 6826
SapI GCTCTTCn'nnn_
Cuts at: 2099 2984 2099
Size: 885 5941
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 3077 3620 5074 6786 3077
Size: 543 1454 1712 3117
Fragments arranged by size:
3117 1712 1454 543
SexAI A'CCwGG_T
Cuts at: 4071 4071
Size: 6826
SfcI C'TryA_G
Cuts at: 826 1045 4109 4174 5309 5990 6181 826
Size: 219 3064 65 1135 681 191 1471
Fragments arranged by size:
3064 1471 1135 681 219 191 65
SfoI GGC'GCC
Cuts at: 3113 3758 3896 3113
Size: 645 138 6043
Fragments arranged by size:
6043 645 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2840 3970 4153 1872
Size: 968 1130 183 4545
Fragments arranged by size:
4545 1130 968 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 4930 5831 6093 820
Size: 197 926 402 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6826
StyI C'CwwG_G
Cuts at: 513 1065 2887 3175 4265 513
Size: 552 1822 288 1090 3074
Fragments arranged by size:
3074 1822 1090 552 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3700 3757 4853 4870 5023 5208 5653
Size: 1255 2120 57 1096 17 153 185 445
Cuts at: 5653 325
Size: 1498
Fragments arranged by size:
2120 1498 1255 1096 445 185 153 57
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 3075 3618
Size: 274 80 33 51 1443 504 594 543
Cuts at: 3618 3771 5072 6784 96
Size: 153 1301 1712 138
Fragments arranged by size:
1712 1443 1301 594 543 504 274 153
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3816 3848 6786 6818 3816
Size: 32 2938 32 3824
Fragments arranged by size:
3824 2938 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 3710 1316
Size: 1185 1209 4432
Fragments arranged by size:
4432 1209 1185
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 2838 3968 4151 1870
Size: 968 1130 183 4545
Fragments arranged by size:
4545 1130 968 183
XmnI GAAnn'nnTTC
Cuts at: 3563 3740 4955 3563
Size: 177 1215 5434
Fragments arranged by size:
5434 1215 177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3994 4634 276
Size: 53 83 186 936 2460 640 2468
Fragments arranged by size:
2468 2460 936 640 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SfiI SrfI SwaI
XcmI