Restriction map for
pFN21AB9973
(Circular) (Six-base) MAPSORT of: pFN21AB9973.seq Check: 9335 from: 1 to: 6367
pFN21AB9973 6367 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2154 2783 3510 2154
Size: 629 727 5011
Fragments arranged by size:
5011 727 629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4177 278
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2214 3710 1937
Size: 277 1496 4594
Fragments arranged by size:
4594 1496 277
Acc65I G'GTAC_C
Cuts at: 3688 3688
Size: 6367
AclI AA'CG_TT
Cuts at: 1563 4494 4867 1563
Size: 2931 373 3063
Fragments arranged by size:
3063 2931 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 2381 4430 5814 6082 1632
Size: 652 97 2049 1384 268 1917
Fragments arranged by size:
2049 1917 1384 652 268 97
AflII C'TTAA_G
Cuts at: 820 1017 2249 820
Size: 197 1232 4938
Fragments arranged by size:
4938 1232 197
AflIII A'CryG_T
Cuts at: 1203 5266 6243 1203
Size: 4063 977 1327
Fragments arranged by size:
4063 1327 977
AgeI A'CCGG_T
Cuts at: 5198 5198
Size: 6367
AhdI GACnn_n'nnGTC
Cuts at: 5096 5096
Size: 6367
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3323 1184
Size: 2139 4228
AlwNI CAG_nnn'CTG
Cuts at: 2155 2164 2440 2725 4110 5682 2155
Size: 9 276 285 1385 1572 2840
Fragments arranged by size:
2840 1572 1385 285 276 9
ApaI G_GGCC'C
Cuts at: 3119 3119
Size: 6367
ApaLI G'TGCA_C
Cuts at: 4424 5580 4424
Size: 1156 5211
ApoI r'AATT_y
Cuts at: 1493 3676 3902 4054 6163 1493
Size: 2183 226 152 2109 1697
Fragments arranged by size:
2183 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4921 160
Size: 872 3889 1606
Fragments arranged by size:
3889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 3295 3692 1870
Size: 73 927 425 397 4545
Fragments arranged by size:
4545 927 425 397 73
BamHI G'GATC_C
Cuts at: 2737 3464 3697 4064 2737
Size: 727 233 367 5040
Fragments arranged by size:
5040 727 367 233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3508 3688 5143 618
Size: 325 217 627 231 1490 180 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 231 217 180
BanII G_rGCy'C
Cuts at: 1391 2057 3015 3119 3359 3686 1391
Size: 666 958 104 240 327 4072
Fragments arranged by size:
4072 958 666 327 240 104
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 6367
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3167 3487 928
Size: 971 1268 320 3808
Fragments arranged by size:
3808 1268 971 320
BbvCI CC'TCA_GC
Cuts at: 3242 3242
Size: 6367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4558 4592 1903
Size: 34 2621 34 3678
Fragments arranged by size:
3678 2621 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4259 5469 4259
Size: 1210 5157
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 3237 4978
Size: 107 122 71 631 605 985 580 1741
Cuts at: 4978 136
Size: 1525
Fragments arranged by size:
1741 1525 985 631 605 580 122 107
71
BglII A'GATC_T
Cuts at: 6362 6362
Size: 6367
BlpI GC'TnA_GC
Cuts at: 3267 3784 3267
Size: 517 5850
Bme1580I G_kGCm'C
Cuts at: 1790 2906 3119 3513 4428 5584 1790
Size: 1116 213 394 915 1156 2573
Fragments arranged by size:
2573 1156 1116 915 394 213
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2798 3507 4098
Size: 416 53 83 399 24 1381 709 591
Cuts at: 4098 5056 442
Size: 958 1753
Fragments arranged by size:
1753 1381 958 709 591 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 3223 3484 2716
Size: 507 261 5599
Fragments arranged by size:
5599 507 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2462 2524 2952 2965 3344 3454 3467
Size: 1178 45 62 428 13 379 110 13
Cuts at: 3467 5027 1239
Size: 1560 2579
Fragments arranged by size:
2579 1560 1178 428 379 110 62 45
13 13
Bpu10I CC'TnA_GC
Cuts at: 3242 3242
Size: 6367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4492 5357 5655 4492
Size: 865 298 5204
Fragments arranged by size:
5204 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 2792 3183 5030 882
Size: 617 678 615 391 1847 2219
Fragments arranged by size:
2219 1847 678 617 615 391
BsaAI yAC'GTr
Cuts at: 493 1204 4158 493
Size: 711 2954 2702
Fragments arranged by size:
2954 2702 711
BsaBI GATnn'nnATC
Cuts at: 6361 6361
Size: 6367
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 4174 4556 5191
Size: 53 83 186 936 486 2155 382 635
Cuts at: 5191 275
Size: 1451
Fragments arranged by size:
2155 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4800 5198 5472 5619 6030 4800
Size: 398 274 147 411 5137
Fragments arranged by size:
5137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 1178
Size: 30 165 30 771 30 5341
Fragments arranged by size:
5341 771 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 3283 3349 3367 1186
Size: 931 1166 66 18 4186
Fragments arranged by size:
4186 1166 931 66 18
BseYI C'CCAG_C
Cuts at: 2050 3023 5570 6066 2050
Size: 973 2547 496 2351
Fragments arranged by size:
2547 2351 973 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2711 1163
Size: 573 618 357 4819
Fragments arranged by size:
4819 618 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3817 4578 5606 1540
Size: 275 186 1816 761 1028 2301
Fragments arranged by size:
2301 1816 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2774 3341 4428 4513 5584 2774
Size: 567 1087 85 1071 3557
Fragments arranged by size:
3557 1087 1071 567 85
BsmI GAATG_Cn'
Cuts at: 2540 3878 3971 2540
Size: 1338 93 4936
Fragments arranged by size:
4936 1338 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2774 2906 3015 3119 3341 3359
Size: 399 267 717 132 109 104 222 18
Cuts at: 3359 3513 3686 4428 4513 5584 1391
Size: 154 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 399 267 222 173
154 132 109 104 85 18
BspEI T'CCGG_A
Cuts at: 6030 6030
Size: 6367
BspHI T'CATG_A
Cuts at: 4256 5986 4256
Size: 1730 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2783 3510 3722 844
Size: 1310 629 727 212 3489
Fragments arranged by size:
3489 1310 727 629 212
BsrBI CCG'CTC
Cuts at: 1394 1823 3596 4254 1394
Size: 429 1773 658 3507
Fragments arranged by size:
3507 1773 658 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 4862 5036 65
Size: 1183 1656 1958 174 1396
Fragments arranged by size:
1958 1656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3079 5011 5198 1700
Size: 110 1269 1932 187 2869
Fragments arranged by size:
2869 1932 1269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6367
BssSI C'ACGA_G
Cuts at: 2188 4427 5439 2188
Size: 2239 1012 3116
Fragments arranged by size:
3116 2239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2514 2725 794
Size: 1361 359 211 4436
Fragments arranged by size:
4436 1361 359 211
BstBI TT'CG_AA
Cuts at: 5171 5171
Size: 6367
BstEII G'GTnAC_C
Cuts at: 1184 2554 1184
Size: 1370 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 2132 2132
Size: 6367
BstYI r'GATC_y
Cuts at: 1972 2233 2737 3216 3464 3697 4064 4449 4466
Size: 261 504 479 248 233 367 385 17
Cuts at: 4466 5918 6004 6016 6362 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 504 479 385 367 346 261
248 233 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2646 513
Size: 552 1581 4234
Fragments arranged by size:
4234 1581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2308 487
Size: 683 56 1082 4546
Fragments arranged by size:
4546 1082 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 2752 3224 3883 4677 4697 2608
Size: 144 472 659 794 20 4278
Fragments arranged by size:
4278 794 659 472 144 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6332
DraI TTT'AAA
Cuts at: 3671 3671
Size: 6367
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 6367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5374 809
Size: 507 4058 1802
Fragments arranged by size:
4058 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2759 3133 3534
Size: 54 1425 50 275 18 929 374 401
Cuts at: 3534 3814 4703 6332 8
Size: 280 889 1629 43
Fragments arranged by size:
1629 1425 929 889 401 374 280 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3814 1537
Size: 275 2002 4090
Fragments arranged by size:
4090 2002 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2357 2382 4297 1353
Size: 1004 25 1915 3423
Fragments arranged by size:
3423 1915 1004 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3586 4954 5328 5474 387
Size: 1064 138 1997 1368 374 146 1280
Fragments arranged by size:
1997 1368 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2284 2381 2417 2462 2524 2952 2965
Size: 393 652 97 36 45 62 428 13
Cuts at: 2965 3344 3454 3467 4430 5027 5814 6082 1239
Size: 379 110 13 963 597 787 268 1524
Fragments arranged by size:
1524 963 787 652 597 428 393 379
268 110 97 62 45 36 13 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2486 3106 3635 2252
Size: 234 620 529 4984
Fragments arranged by size:
4984 620 529 234
EcoO109I rG'GnC_Cy
Cuts at: 2859 2914 3116 3238 3402 2859
Size: 55 202 122 164 5824
Fragments arranged by size:
5824 202 164 122 55
EcoRI G'AATT_C
Cuts at: 3676 4054 3676
Size: 378 5989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2476 2386
Size: 90 6277
FspI TGC'GCA
Cuts at: 4873 4873
Size: 6367
HaeII r_GCGC'y
Cuts at: 1678 2022 2032 5514 1678
Size: 344 10 3482 2531
Fragments arranged by size:
3482 2531 344 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2158 2190 2597
Size: 32 135 32 521 32 33 32 407
Cuts at: 2597 2629 3154 3158 3186 3190 3440 3472 5008
Size: 32 525 4 28 4 250 32 1536
Cuts at: 5008 5040 5082 5114 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1536 525 521 407 250 135 42
33 32 32 32 32 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3711 3957 669
Size: 988 281 1773 246 3079
Fragments arranged by size:
3079 1773 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6367
HpaI GTT'AAC
Cuts at: 3957 3957
Size: 6367
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 6367
KpnI G_GTAC'C
Cuts at: 3692 3692
Size: 6367
MfeI C'AATT_G
Cuts at: 3966 3966
Size: 6367
MluI A'CGCG_T
Cuts at: 6243 6243
Size: 6367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 3520 5481 5665 1305
Size: 786 1429 1961 184 2007
Fragments arranged by size:
2007 1961 1429 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3135 10
Size: 54 1425 343 1303 3242
Fragments arranged by size:
3242 1425 1303 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4325 4684 4843 518
Size: 648 3159 359 159 2042
Fragments arranged by size:
3159 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2338 2758 3783 4460 5608 5853 1606
Size: 555 177 420 1025 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 555 420 245 177
NaeI GCC'GGC
Cuts at: 3081 3081
Size: 6367
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 6367
NcoI C'CATG_G
Cuts at: 513 1065 2646 513
Size: 552 1581 4234
Fragments arranged by size:
4234 1581 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6367
NgoMIV G'CCGG_C
Cuts at: 3079 3079
Size: 6367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6367
NotI GC'GGCC_GC
Cuts at: 3814 3814
Size: 6367
NspI r_CATG'y
Cuts at: 1132 1229 3004 3725 5270 1132
Size: 97 1775 721 1545 2229
Fragments arranged by size:
2229 1775 1545 721 97
PasI CC'CwG_GG
Cuts at: 1348 3108 3235 1348
Size: 1760 127 4480
Fragments arranged by size:
4480 1760 127
PciI A'CATG_T
Cuts at: 5266 5266
Size: 6367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2046 2440 2551 3505 1108
Size: 938 394 111 954 3970
Fragments arranged by size:
3970 954 938 394 111
PfoI T'CCnGG_A
Cuts at: 3483 3483
Size: 6367
PmeI GTTT'AAAC
Cuts at: 3671 3671
Size: 6367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 5949 5981 4436
Size: 32 1481 32 4822
Fragments arranged by size:
4822 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2914 2914
Size: 6367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6367
PsiI TTA'TAA
Cuts at: 3937 3937
Size: 6367
PspOMI G'GGCC_C
Cuts at: 3115 3115
Size: 6367
PstI C_TGCA'G
Cuts at: 830 2151 2160 2343 3719 830
Size: 1321 9 183 1376 3478
Fragments arranged by size:
3478 1376 1321 183 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6367
PvuII CAG'CTG
Cuts at: 2338 2338
Size: 6367
SalI G'TCGA_C
Cuts at: 1936 3709 1936
Size: 1773 4594
SanDI GG'GwC_CC
Cuts at: 2914 2914
Size: 6367
SapI GCTCTTCn'nnn_
Cuts at: 2357 2382 2357
Size: 25 6342
SbfI CC_TGCA'GG
Cuts at: 3719 3719
Size: 6367
ScaI AGT'ACT
Cuts at: 2117 4615 6327 2117
Size: 2498 1712 2157
Fragments arranged by size:
2498 2157 1712
SfcI C'TryA_G
Cuts at: 826 1045 2119 2147 2156 2339 3556 3715 4850
Size: 219 1074 28 9 183 1217 159 1135
Cuts at: 4850 5531 5722 826
Size: 681 191 1471
Fragments arranged by size:
1471 1217 1135 1074 681 219 191 183
159 28 9
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 6367
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6367
SmaI CCC'GGG
Cuts at: 1872 2872 3694 1872
Size: 1000 822 4545
Fragments arranged by size:
4545 1000 822
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 4471 5372 5634 820
Size: 197 926 306 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 493
Size: 3665 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6367
SphI G_CATG'C
Cuts at: 1132 3004 3725 1132
Size: 1872 721 3774
Fragments arranged by size:
3774 1872 721
SspI AAT'ATT
Cuts at: 5 52 2500 4291 5
Size: 47 2448 1791 2081
Fragments arranged by size:
2448 2081 1791 47
StuI AGG'CCT
Cuts at: 1517 2402 3546 1517
Size: 885 1144 4338
Fragments arranged by size:
4338 1144 885
StyI C'CwwG_G
Cuts at: 513 1065 2376 2646 2959 3313 3606 3806 513
Size: 552 1311 270 313 354 293 200 3074
Fragments arranged by size:
3074 1311 552 354 313 293 270 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 4394 4411 4564 4749 5194 325
Size: 1255 1199 1615 17 153 185 445 1498
Fragments arranged by size:
1615 1498 1255 1199 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 4613 6325
Size: 274 80 33 51 1443 138 2498 1712
Cuts at: 6325 96
Size: 138
Fragments arranged by size:
2498 1712 1443 274 138 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2722 2754 6327 6359 2174
Size: 32 516 32 3573 32 2182
Fragments arranged by size:
3573 2182 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6367
XbaI T'CTAG_A
Cuts at: 3703 3703
Size: 6367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2548 2653 3520 2047
Size: 501 105 867 4894
Fragments arranged by size:
4894 867 501 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6367
XmaI C'CCGG_G
Cuts at: 1870 2870 3692 1870
Size: 1000 822 4545
Fragments arranged by size:
4545 1000 822
XmnI GAAnn'nnTTC
Cuts at: 2501 4496 2501
Size: 1995 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4175 276
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SexAI
SfiI SrfI SwaI