Restriction map for
pFN21AB9969
(Circular) (Six-base) MAPSORT of: pFN21AB9969.seq Check: 864 from: 1 to: 6361
pFN21AB9969 6361 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2123 2731 2123
Size: 608 5753
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2306 2384 2447 3584 4424 5808 6076
Size: 633 41 78 63 1137 840 1384 268
Cuts at: 6076 1632
Size: 1917
Fragments arranged by size:
1917 1384 1137 840 633 268 78 63
41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 2538 5260 6237 1203
Size: 1335 2722 977 1327
Fragments arranged by size:
2722 1335 1327 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2672 1184
Size: 1488 4873
AlwNI CAG_nnn'CTG
Cuts at: 2671 3508 4104 5676 2671
Size: 837 596 1572 3356
Fragments arranged by size:
3356 1572 837 596
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 3670 3896 4048 6157 1493
Size: 2177 226 152 2109 1697
Fragments arranged by size:
2177 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 3094 3300 3429 3686 1870
Size: 73 903 248 206 129 257 4545
Fragments arranged by size:
4545 903 257 248 206 129 73
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2756 2839 2854 3220 3419
Size: 325 217 627 969 83 15 366 199
Cuts at: 3419 3682 5137 618
Size: 263 1455 1842
Fragments arranged by size:
1842 1455 969 627 366 325 263 217
199 83 15
BanII G_rGCy'C
Cuts at: 1391 2210 3101 3680 1391
Size: 819 891 579 4072
Fragments arranged by size:
4072 891 819 579
BbeI G_GCGC'C
Cuts at: 2760 3423 2760
Size: 663 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3269 928
Size: 971 1370 4020
Fragments arranged by size:
4020 1370 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2680 2780 2922
Size: 107 122 71 631 605 1008 100 142
Cuts at: 2922 3431 3449 4972 136
Size: 509 18 1523 1525
Fragments arranged by size:
1525 1523 1008 631 605 509 142 122
107 100 71 18
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 2421 3002 3778 2421
Size: 581 776 5004
Fragments arranged by size:
5004 776 581
Bme1580I G_kGCm'C
Cuts at: 1790 2844 3492 4422 5578 1790
Size: 1054 648 930 1156 2573
Fragments arranged by size:
2573 1156 1054 930 648
BmgBI CAC'GTC
Cuts at: 1318 3556 1318
Size: 2238 4123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4092 5050 442
Size: 416 53 83 399 24 2675 958 1753
Fragments arranged by size:
2675 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2246 2356 2246
Size: 110 6251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 2339 2549 3137 5021 1239
Size: 1087 13 210 588 1884 2579
Fragments arranged by size:
2579 1884 1087 588 210 13
Bpu10I CC'TnA_GC
Cuts at: 3185 3185
Size: 6361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5024 882
Size: 617 3525 2219
Fragments arranged by size:
3525 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2539 4152 493
Size: 711 1335 1613 2702
Fragments arranged by size:
2702 1613 1335 711
BsaBI GATnn'nnATC
Cuts at: 6355 6355
Size: 6361
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2400 2502 2757
Size: 53 83 186 936 633 234 102 255
Cuts at: 2757 2907 3420 4168 4550 5185 275
Size: 150 513 748 382 635 1451
Fragments arranged by size:
1451 936 748 635 633 513 382 255
234 186 150 102 83 53
BsaWI w'CCGG_w
Cuts at: 2088 4794 5192 5466 5613 6024 2088
Size: 2706 398 274 147 411 2425
Fragments arranged by size:
2706 2425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 2591 2621 2999
Size: 30 165 30 1074 30 84 30 378
Cuts at: 2999 3029 1178
Size: 30 4510
Fragments arranged by size:
4510 1074 378 165 84 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2585 2970 1186
Size: 853 546 385 4577
Fragments arranged by size:
4577 853 546 385
BseYI C'CCAG_C
Cuts at: 2702 2768 2961 3164 5564 6060 2702
Size: 66 193 203 2400 496 3003
Fragments arranged by size:
3003 2400 496 203 193 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2747 3317 1163
Size: 573 1011 570 4207
Fragments arranged by size:
4207 1011 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2891 3101 3627 4422 4507 5578 2891
Size: 210 526 795 85 1071 3674
Fragments arranged by size:
3674 1071 795 526 210 85
BsmI GAATG_Cn'
Cuts at: 3872 3965 3872
Size: 93 6268
BsmBI CGTCTCn'nnnn_
Cuts at: 2217 2217
Size: 6361
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2844 2891 3101 3492 3627 3680
Size: 399 420 634 47 210 391 135 53
Cuts at: 3680 4422 4507 5578 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 420 399 391 210
135 85 53 47
BspEI T'CCGG_A
Cuts at: 2088 6024 2088
Size: 3936 2425
BspHI T'CATG_A
Cuts at: 2898 4250 5980 2898
Size: 1352 1730 3279
Fragments arranged by size:
3279 1730 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2123 2731 3716 844
Size: 1279 608 985 3489
Fragments arranged by size:
3489 1279 985 608
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3130 4856 5030 65
Size: 1183 1882 1726 174 1396
Fragments arranged by size:
1882 1726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2836 3110 5005 5192 1700
Size: 110 224 802 274 1895 187 2869
Fragments arranged by size:
2869 1895 802 274 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6361
BssHII G'CGCG_C
Cuts at: 1925 2719 1925
Size: 794 5567
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 2734 794
Size: 1877 63 4421
Fragments arranged by size:
4421 1877 63
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 3200 1184
Size: 2016 4345
BstXI CCAn_nnnn'nTGG
Cuts at: 3164 3164
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 2259 2709 3691 4058 4443 4460 5912 5998
Size: 287 450 982 367 385 17 1452 86
Cuts at: 5998 6010 6356 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 982 450 385 367 346 287
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3225 3225
Size: 6361
BtgI C'CryG_G
Cuts at: 513 1065 2103 2613 2628 2939 3102 513
Size: 552 1038 510 15 311 163 3772
Fragments arranged by size:
3772 1038 552 510 311 163 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2083 3249 3330 487
Size: 683 56 857 1166 81 3518
Fragments arranged by size:
3518 1166 857 683 81 56
BtsI GCAGTG_nn'
Cuts at: 2612 3328 3877 4671 4691 2612
Size: 716 549 794 20 4282
Fragments arranged by size:
4282 794 716 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5368 809
Size: 507 4052 1802
Fragments arranged by size:
4052 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2616 2745
Size: 54 1425 50 275 18 360 426 129
Cuts at: 2745 3127 3396 3450 3483 3808 4697 6326 8
Size: 382 269 54 33 325 889 1629 43
Fragments arranged by size:
1629 1425 889 426 382 360 325 275
269 129 54 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2438 3350 3652 4291 1353
Size: 884 201 912 302 639 3423
Fragments arranged by size:
3423 912 884 639 302 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2884 3589 4948 5322 5468 387
Size: 1064 138 1295 705 1359 374 146 1280
Fragments arranged by size:
1359 1295 1280 1064 705 374 146 138
EcoICRI GAG'CTC
Cuts at: 3099 3099
Size: 6361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2306 2326 2339 2384 2447 2549
Size: 393 633 41 20 13 45 63 102
Cuts at: 2549 3137 3584 4424 5021 5808 6076 1239
Size: 588 447 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 633 597 588 447 393
268 102 63 45 41 20 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3383 3383
Size: 6361
EcoO109I rG'GnC_Cy
Cuts at: 3471 3505 3471
Size: 34 6327
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 6361
FspI TGC'GCA
Cuts at: 3035 4867 3035
Size: 1832 4529
HaeII r_GCGC'y
Cuts at: 1678 2584 2760 2775 3423 3642 5508 1678
Size: 906 176 15 648 219 1866 2531
Fragments arranged by size:
2531 1866 906 648 219 176 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2999 3031 3073
Size: 32 135 32 679 32 716 32 42
Cuts at: 3073 3105 3526 3558 5002 5034 5076 5108 1373
Size: 32 421 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 716 679 421 135 42 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3705 3951 669
Size: 988 281 1767 246 3079
Fragments arranged by size:
3079 1767 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2432 748
Size: 1684 4677
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KasI G'GCGC_C
Cuts at: 2756 3419 2756
Size: 663 5698
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3960 3960
Size: 6361
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 2817 2832 3042 5475 5659 1305
Size: 1263 249 15 210 2433 184 2007
Fragments arranged by size:
2433 2007 1263 249 210 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2618 2747 3398 10
Size: 54 1425 343 360 426 129 651 2973
Fragments arranged by size:
2973 1425 651 426 360 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 3630 4319 4678 4837 518
Size: 648 936 1528 689 359 159 2042
Fragments arranged by size:
2042 1528 936 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2317 2630 2677 3456 3562 3777 4454
Size: 666 45 313 47 779 106 215 677
Cuts at: 4454 5602 5847 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 779 677 666 313 245 215
106 47 45
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 6361
NarI GG'CG_CC
Cuts at: 2757 3420 2757
Size: 663 5698
NcoI C'CATG_G
Cuts at: 513 1065 2103 3102 513
Size: 552 1038 999 3772
Fragments arranged by size:
3772 1038 999 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 3719 5264 1132
Size: 97 2490 1545 2229
Fragments arranged by size:
2490 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3502 1348
Size: 2154 4207
PciI A'CATG_T
Cuts at: 5260 5260
Size: 6361
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3508 1108
Size: 2400 3961
PfoI T'CCnGG_A
Cuts at: 2712 3378 2712
Size: 666 5695
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 5943 5975 4430
Size: 32 1481 32 4816
Fragments arranged by size:
4816 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2412 1613
Size: 799 5562
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PspXI vC'TCGA_Gb
Cuts at: 3094 3094
Size: 6361
PstI C_TGCA'G
Cuts at: 830 2493 3348 3713 830
Size: 1663 855 365 3478
Fragments arranged by size:
3478 1663 855 365
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 2272 2317 3562 2272
Size: 45 1245 5071
Fragments arranged by size:
5071 1245 45
SacI G_AGCT'C
Cuts at: 3101 3101
Size: 6361
SacII CC_GC'GG
Cuts at: 2631 2631
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SapI GCTCTTCn'nnn_
Cuts at: 2438 3350 2438
Size: 912 5449
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 2474 4609 6321 2474
Size: 2135 1712 2514
Fragments arranged by size:
2514 2135 1712
SexAI A'CCwGG_T
Cuts at: 2796 2796
Size: 6361
SfcI C'TryA_G
Cuts at: 826 1045 2489 2932 3344 3475 3709 4844 5525
Size: 219 1444 443 412 131 234 1135 681
Cuts at: 5525 5716 826
Size: 191 1471
Fragments arranged by size:
1471 1444 1135 681 443 412 234 219
191 131
SfoI GGC'GCC
Cuts at: 2758 3421 2758
Size: 663 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3302 3431 3688 1872
Size: 1430 129 257 4545
Fragments arranged by size:
4545 1430 257 129
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3094 4465 5366 5628 820
Size: 197 926 1151 1371 901 262 1553
Fragments arranged by size:
1553 1371 1151 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 2690 1517
Size: 1173 5188
StyI C'CwwG_G
Cuts at: 513 1065 2103 2643 3102 3604 3800 513
Size: 552 1038 540 459 502 196 3074
Fragments arranged by size:
3074 1038 552 540 502 459 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2908 4388 4405 4558 4743 5188 325
Size: 1255 1328 1480 17 153 185 445 1498
Fragments arranged by size:
1498 1480 1328 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2493 4607
Size: 274 80 33 51 1443 495 21 2114
Cuts at: 4607 6319 96
Size: 1712 138
Fragments arranged by size:
2114 1712 1443 495 274 138 80 51
33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 6321 6353 2591
Size: 32 3698 32 2599
Fragments arranged by size:
3698 2599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 2794
Size: 6361
XhoI C'TCGA_G
Cuts at: 1943 3094 1943
Size: 1151 5210
XmaI C'CCGG_G
Cuts at: 1870 3300 3429 3686 1870
Size: 1430 129 257 4545
Fragments arranged by size:
4545 1430 257 129
XmnI GAAnn'nnTTC
Cuts at: 4490 4490
Size: 6361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspOMI PsrI RsrII SanDI SfiI
SrfI SwaI