Restriction map for
pFN21AB9968
(Circular) (Six-base) MAPSORT of: pFN21AB9968.seq Check: 423 from: 1 to: 6805
pFN21AB9968 6805 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3243 3243
Size: 6805
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4615 278
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2814 3700 4148 1937
Size: 877 886 448 4594
Fragments arranged by size:
4594 886 877 448
Acc65I G'GTAC_C
Cuts at: 4126 4126
Size: 6805
AclI AA'CG_TT
Cuts at: 1563 4932 5305 1563
Size: 3369 373 3063
Fragments arranged by size:
3369 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2194 2966 3989 4868 6252 6520 1632
Size: 562 772 1023 879 1384 268 1917
Fragments arranged by size:
1917 1384 1023 879 772 562 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6608
AflIII A'CryG_T
Cuts at: 1203 3156 3213 5704 6681 1203
Size: 1953 57 2491 977 1327
Fragments arranged by size:
2491 1953 1327 977 57
AgeI A'CCGG_T
Cuts at: 5636 5636
Size: 6805
AhdI GACnn_n'nnGTC
Cuts at: 5534 5534
Size: 6805
AleI CACnn'nnGTG
Cuts at: 1166 3569 3894 1166
Size: 2403 325 4077
Fragments arranged by size:
4077 2403 325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2700 3912 1184
Size: 1516 1212 4077
Fragments arranged by size:
4077 1516 1212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 6773
AlwNI CAG_nnn'CTG
Cuts at: 2143 4548 6120 2143
Size: 2405 1572 2828
Fragments arranged by size:
2828 2405 1572
ApaI G_GGCC'C
Cuts at: 2331 2994 2331
Size: 663 6142
ApaLI G'TGCA_C
Cuts at: 2981 3887 4862 6018 2981
Size: 906 975 1156 3768
Fragments arranged by size:
3768 1156 975 906
ApoI r'AATT_y
Cuts at: 1493 2553 2612 3098 4043 4114 4340 4492 6601
Size: 1060 59 486 945 71 226 152 2109
Cuts at: 6601 1493
Size: 1697
Fragments arranged by size:
2109 1697 1060 945 486 226 152 71
59
AseI AT'TA_AT
Cuts at: 160 1032 5359 160
Size: 872 4327 1606
Fragments arranged by size:
4327 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 3179 4130 1870
Size: 73 182 1054 951 4545
Fragments arranged by size:
4545 1054 951 182 73
BamHI G'GATC_C
Cuts at: 4135 4502 4135
Size: 367 6438
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 3032 3251 4126 5581
Size: 325 217 627 342 903 219 875 1455
Cuts at: 5581 618
Size: 1842
Fragments arranged by size:
1842 1455 903 875 627 342 325 219
217
BanII G_rGCy'C
Cuts at: 1391 2013 2062 2331 2994 3638 4124 1391
Size: 622 49 269 663 644 486 4072
Fragments arranged by size:
4072 663 644 622 486 269 49
BbeI G_GCGC'C
Cuts at: 2133 2133
Size: 6805
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5834
BbvCI CC'TCA_GC
Cuts at: 3932 3932
Size: 6805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2063 2097 4996 5030 1903
Size: 34 126 34 2899 34 3678
Fragments arranged by size:
3678 2899 126 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4697 5907 4697
Size: 1210 5595
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6805
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3030 3059 5416
Size: 107 122 71 631 605 1358 29 2357
Cuts at: 5416 136
Size: 1525
Fragments arranged by size:
2357 1525 1358 631 605 122 107 71
29
BglII A'GATC_T
Cuts at: 6800 6800
Size: 6805
BlpI GC'TnA_GC
Cuts at: 2309 4222 2309
Size: 1913 4892
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2985 2994 3035 3891 4866 6022 1790
Size: 541 654 9 41 856 975 1156 2573
Fragments arranged by size:
2573 1156 975 856 654 541 41 9
BmgBI CAC'GTC
Cuts at: 1318 3214 1318
Size: 1896 4909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4536 5494 442
Size: 416 53 83 399 24 3119 958 1753
Fragments arranged by size:
3119 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6805
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 6805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2680 3245 3302 3556 5465 1239
Size: 1145 296 565 57 254 1909 2579
Fragments arranged by size:
2579 1909 1145 565 296 254 57
Bpu10I CC'TnA_GC
Cuts at: 3932 3932
Size: 6805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2610 2939 4930 5795 6093 2610
Size: 329 1991 865 298 3322
Fragments arranged by size:
3322 1991 865 329 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2069 2887 4003 5468 882
Size: 617 570 818 1116 1465 2219
Fragments arranged by size:
2219 1465 1116 818 617 570
BsaAI yAC'GTr
Cuts at: 493 1204 4596 493
Size: 711 3392 2702
Fragments arranged by size:
3392 2702 711
BsaBI GATnn'nnATC
Cuts at: 2378 6799 2378
Size: 4421 2384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 4612 4994 5629
Size: 53 83 186 936 597 2482 382 635
Cuts at: 5629 275
Size: 1451
Fragments arranged by size:
2482 1451 936 635 597 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2119 2826 3312 5238 5636 5910 6057 6468 2119
Size: 707 486 1926 398 274 147 411 2456
Fragments arranged by size:
2456 1926 707 486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2666 2696 3203
Size: 30 165 30 597 30 636 30 507
Cuts at: 3203 3233 3275 3305 1178
Size: 30 42 30 4678
Fragments arranged by size:
4678 636 597 507 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2168 2171 2294 2381 2384 2429 3299
Size: 979 3 3 123 87 3 45 870
Cuts at: 3299 3302 3761 1186
Size: 3 459 4230
Fragments arranged by size:
4230 979 870 459 123 87 45 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2136 2987 3905 6008 6504 2136
Size: 851 918 2103 496 2437
Fragments arranged by size:
2437 2103 918 851 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2876 3608 3866 1163
Size: 573 1140 732 258 4102
Fragments arranged by size:
4102 1140 732 573 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4255 5016 6044 1540
Size: 275 186 2254 761 1028 2301
Fragments arranged by size:
2301 2254 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2361 2985 3891 4866 4951 6022 2190
Size: 171 624 906 975 85 1071 2973
Fragments arranged by size:
2973 1071 975 906 624 171 85
BsmI GAATG_Cn'
Cuts at: 4316 4409 4316
Size: 93 6712
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2062 2190 2331 2361 2985 2994
Size: 399 223 49 128 141 30 624 9
Cuts at: 2994 3035 3638 3891 4124 4866 4951 6022 1391
Size: 41 603 253 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 624 603 399 253 233
223 141 128 85 49 41 30 9
BspEI T'CCGG_A
Cuts at: 2119 2826 3312 6468 2119
Size: 707 486 3156 2456
Fragments arranged by size:
3156 2456 707 486
BspHI T'CATG_A
Cuts at: 3318 4694 6424 3318
Size: 1376 1730 3699
Fragments arranged by size:
3699 1730 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 3243 3742 4160 844
Size: 1460 939 499 418 3489
Fragments arranged by size:
3489 1460 939 499 418
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 4692 1394
Size: 429 227 2642 3507
Fragments arranged by size:
3507 2642 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 2426 5300 5474 65
Size: 1183 1178 2874 174 1396
Fragments arranged by size:
2874 1396 1183 1178 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3349 5449 5636 1700
Size: 110 1539 2100 187 2869
Fragments arranged by size:
2869 2100 1539 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6805
BssSI C'ACGA_G
Cuts at: 2183 4865 5877 2183
Size: 2682 1012 3111
Fragments arranged by size:
3111 2682 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3244 794
Size: 2450 4355
BstBI TT'CG_AA
Cuts at: 5609 5609
Size: 6805
BstEII G'GTnAC_C
Cuts at: 1184 2316 1184
Size: 1132 5673
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2549 2353
Size: 196 6609
BstYI r'GATC_y
Cuts at: 1972 2373 2384 4135 4502 4887 4904 6356 6442
Size: 401 11 1751 367 385 17 1452 86
Cuts at: 6442 6454 6800 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1751 1452 401 385 367 346 86
17 12 11
BstZ17I GTA'TAC
Cuts at: 3701 3701
Size: 6805
Bsu36I CC'TnA_GG
Cuts at: 2110 2323 2110
Size: 213 6592
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 3781 513
Size: 552 939 348 1429 3537
Fragments arranged by size:
3537 1429 939 552 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3478 3820 487
Size: 683 56 2252 342 3472
Fragments arranged by size:
3472 2252 683 342 56
BtsI GCAGTG_nn'
Cuts at: 2079 3889 3901 4321 5115 5135 2079
Size: 1810 12 420 794 20 3749
Fragments arranged by size:
3749 1810 794 420 20 12
ClaI AT'CG_AT
Cuts at: 1554 3048 1554
Size: 1494 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6770
DraI TTT'AAA
Cuts at: 4109 4109
Size: 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3713 5812 809
Size: 507 2397 2099 1802
Fragments arranged by size:
2397 2099 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3060 3623 4252
Size: 54 1425 50 275 18 1230 563 629
Cuts at: 4252 5141 6770 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1230 889 629 563 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4252 1537
Size: 275 2440 4090
Fragments arranged by size:
4090 2440 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3986 4735 1353
Size: 2633 749 3423
Fragments arranged by size:
3423 2633 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2377 5392 5766 5912 387
Size: 1064 138 788 3015 374 146 1280
Fragments arranged by size:
3015 1280 1064 788 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2194 2384 2680 2966 3245 3302 3556
Size: 393 562 190 296 286 279 57 254
Cuts at: 3556 3989 4868 5465 6252 6520 1239
Size: 433 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 562 433 393 296
286 279 268 254 190 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 6805
EcoO109I rG'GnC_Cy
Cuts at: 2114 2327 2630 2114
Size: 213 303 6289
Fragments arranged by size:
6289 303 213
EcoRI G'AATT_C
Cuts at: 2553 4043 4114 4492 2553
Size: 1490 71 378 4866
Fragments arranged by size:
4866 1490 378 71
EcoRV GAT'ATC
Cuts at: 2498 2498
Size: 6805
FspI TGC'GCA
Cuts at: 5311 5311
Size: 6805
HaeII r_GCGC'y
Cuts at: 1678 2133 3127 5952 1678
Size: 455 994 2825 2531
Fragments arranged by size:
2825 2531 994 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2666 2698 3203 3235 5446
Size: 32 135 32 1094 32 505 32 2211
Cuts at: 5446 5478 5520 5552 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2211 1094 505 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2650 2703 2815 4149 4395 669
Size: 988 281 712 53 112 1334 246 3079
Fragments arranged by size:
3079 1334 988 712 281 246 112 53
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6805
HpaI GTT'AAC
Cuts at: 2650 2703 4395 2650
Size: 53 1692 5060
Fragments arranged by size:
5060 1692 53
KasI G'GCGC_C
Cuts at: 2129 2129
Size: 6805
KpnI G_GTAC'C
Cuts at: 4130 4130
Size: 6805
MfeI C'AATT_G
Cuts at: 4404 4404
Size: 6805
MluI A'CGCG_T
Cuts at: 6681 6681
Size: 6805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5919 6103 1305
Size: 4614 184 2007
Fragments arranged by size:
4614 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 3625 10
Size: 54 1425 343 1230 563 3190
Fragments arranged by size:
3190 1425 1230 563 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2696 2783 2939 3569 3884 3894
Size: 648 1185 345 87 156 630 315 10
Cuts at: 3894 4763 5122 5281 518
Size: 869 359 159 2042
Fragments arranged by size:
2042 1185 869 648 630 359 345 315
159 156 87 10
MspA1I CmG'CkG
Cuts at: 1606 2143 2167 2890 2904 4221 4898 6046 6291
Size: 537 24 723 14 1317 677 1148 245
Cuts at: 6291 1606
Size: 2120
Fragments arranged by size:
2120 1317 1148 723 677 537 245 24
14
NaeI GCC'GGC
Cuts at: 3351 3351
Size: 6805
NarI GG'CG_CC
Cuts at: 2130 2130
Size: 6805
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5314
Fragments arranged by size:
5314 939 552
NdeI CA'TA_TG
Cuts at: 387 4017 387
Size: 3630 3175
NgoMIV G'CCGG_C
Cuts at: 3349 3349
Size: 6805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6805
NotI GC'GGCC_GC
Cuts at: 4252 4252
Size: 6805
NspI r_CATG'y
Cuts at: 1132 1229 4163 5708 1132
Size: 97 2934 1545 2229
Fragments arranged by size:
2934 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3502 1348
Size: 2154 4651
PciI A'CATG_T
Cuts at: 5704 5704
Size: 6805
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6805
PmeI GTTT'AAAC
Cuts at: 4109 4109
Size: 6805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 4874 4906 6387 6419 3275
Size: 32 1567 32 1481 32 3661
Fragments arranged by size:
3661 1567 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2630 2630
Size: 6805
PshAI GACnn'nnGTC
Cuts at: 1613 2693 3308 1613
Size: 1080 615 5110
Fragments arranged by size:
5110 1080 615
PsiI TTA'TAA
Cuts at: 4375 4375
Size: 6805
PspOMI G'GGCC_C
Cuts at: 2327 2990 2327
Size: 663 6142
PspXI vC'TCGA_Gb
Cuts at: 3179 3179
Size: 6805
PstI C_TGCA'G
Cuts at: 830 3240 3896 4157 830
Size: 2410 656 261 3478
Fragments arranged by size:
3478 2410 656 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6805
PvuII CAG'CTG
Cuts at: 2890 2904 2890
Size: 14 6791
SalI G'TCGA_C
Cuts at: 1936 2813 4147 1936
Size: 877 1334 4594
Fragments arranged by size:
4594 1334 877
SbfI CC_TGCA'GG
Cuts at: 4157 4157
Size: 6805
ScaI AGT'ACT
Cuts at: 2744 3185 5053 6765 2744
Size: 441 1868 1712 2784
Fragments arranged by size:
2784 1868 1712 441
SfcI C'TryA_G
Cuts at: 826 1045 3007 3236 3892 4153 5288 5969 6160
Size: 219 1962 229 656 261 1135 681 191
Cuts at: 6160 826
Size: 1471
Fragments arranged by size:
1962 1471 1135 681 656 261 229 219
191
SfoI GGC'GCC
Cuts at: 2131 2131
Size: 6805
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6805
SmaI CCC'GGG
Cuts at: 1872 2127 4132 1872
Size: 255 2005 4545
Fragments arranged by size:
4545 2005 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2625 2954 3179 4909 5810 6072
Size: 197 926 682 329 225 1730 901 262
Cuts at: 6072 820
Size: 1553
Fragments arranged by size:
1730 1553 926 901 682 329 262 225
197
SnaBI TAC'GTA
Cuts at: 493 4596 493
Size: 4103 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6805
SphI G_CATG'C
Cuts at: 1132 4163 1132
Size: 3031 3774
SspI AAT'ATT
Cuts at: 5 52 4729 5
Size: 47 4677 2081
Fragments arranged by size:
4677 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6805
StyI C'CwwG_G
Cuts at: 513 1065 2004 2755 4244 513
Size: 552 939 751 1489 3074
Fragments arranged by size:
3074 1489 939 751 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3275 3515 4832 4849 5002 5187 5632
Size: 1255 1695 240 1317 17 153 185 445
Cuts at: 5632 325
Size: 1498
Fragments arranged by size:
1695 1498 1317 1255 445 240 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 3153 3183
Size: 274 80 33 51 1443 765 411 30
Cuts at: 3183 5051 6763 96
Size: 1868 1712 138
Fragments arranged by size:
1868 1712 1443 765 411 274 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 6765 6797 2000
Size: 32 4733 32 2008
Fragments arranged by size:
4733 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6805
XbaI T'CTAG_A
Cuts at: 4141 4141
Size: 6805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2272
Size: 6805
XhoI C'TCGA_G
Cuts at: 1943 3179 1943
Size: 1236 5569
XmaI C'CCGG_G
Cuts at: 1870 2125 4130 1870
Size: 255 2005 4545
Fragments arranged by size:
4545 2005 255
XmnI GAAnn'nnTTC
Cuts at: 2612 4934 2612
Size: 2322 4483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4613 276
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII
EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI