Restriction map for
pFN21AB9967
(Circular) (Six-base) MAPSORT of: pFN21AB9967.seq Check: 1579 from: 1 to: 6358
pFN21AB9967 6358 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2373 4168 278
Size: 53 83 186 936 837 1795 2468
Fragments arranged by size:
2468 1795 936 837 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3701 1937
Size: 436 1328 4594
Fragments arranged by size:
4594 1328 436
Acc65I G'GTAC_C
Cuts at: 3256 3679 3256
Size: 423 5935
AclI AA'CG_TT
Cuts at: 1563 2517 4485 4858 1563
Size: 954 1968 373 3063
Fragments arranged by size:
3063 1968 954 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 2904 2990 4421 5805 6073 1632
Size: 904 368 86 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 904 368 268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 5257 6234 1203
Size: 4054 977 1327
Fragments arranged by size:
4054 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 2387 1166
Size: 1221 5137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 2187 2769 4101 5673 2187
Size: 582 1332 1572 2872
Fragments arranged by size:
2872 1572 1332 582
ApaI G_GGCC'C
Cuts at: 2052 2052
Size: 6358
ApaLI G'TGCA_C
Cuts at: 2710 4415 5571 2710
Size: 1705 1156 3497
Fragments arranged by size:
3497 1705 1156
ApoI r'AATT_y
Cuts at: 1493 2664 3147 3305 3667 3893 4045 6154 1493
Size: 1171 483 158 362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1171 483 362 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 3683 1870
Size: 73 345 1395 4545
Fragments arranged by size:
4545 1395 345 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2428 2797 3256 3679
Size: 325 217 627 446 195 369 459 423
Cuts at: 3679 5134 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 459 446 423 369 325
217 195
BanII G_rGCy'C
Cuts at: 1391 2052 3357 3677 1391
Size: 661 1305 320 4072
Fragments arranged by size:
4072 1305 661 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3334 928
Size: 971 1435 3952
Fragments arranged by size:
3952 1435 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2135 2169 4549 4583 1903
Size: 34 50 34 114 34 2380 34 3678
Fragments arranged by size:
3678 2380 114 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2349 4969 136
Size: 107 122 71 631 605 677 2620 1525
Fragments arranged by size:
2620 1525 677 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2625 3590 6353 2625
Size: 965 2763 2630
Fragments arranged by size:
2763 2630 965
BlpI GC'TnA_GC
Cuts at: 2078 3351 3775 2078
Size: 1273 424 4661
Fragments arranged by size:
4661 1273 424
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2238 2433 2714 4419 5575 1790
Size: 262 186 195 281 1705 1156 2573
Fragments arranged by size:
2573 1705 1156 281 262 195 186
BmgBI CAC'GTC
Cuts at: 1318 2311 1318
Size: 993 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4089 5047 442
Size: 416 53 83 399 24 2672 958 1753
Fragments arranged by size:
2672 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2348 3391 5018 1239
Size: 1076 33 1043 1627 2579
Fragments arranged by size:
2579 1627 1076 1043 33
Bpu10I CC'TnA_GC
Cuts at: 2177 2177
Size: 6358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4483 5348 5646 4483
Size: 865 298 5195
Fragments arranged by size:
5195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5021 882
Size: 617 3522 2219
Fragments arranged by size:
3522 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4149 493
Size: 711 2945 2702
Fragments arranged by size:
2945 2702 711
BsaBI GATnn'nnATC
Cuts at: 3506 6352 3506
Size: 2846 3512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2370 4165 4547
Size: 53 83 186 936 633 204 1795 382
Cuts at: 4547 5182 275
Size: 635 1451
Fragments arranged by size:
1795 1451 936 635 633 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 4791 5189 5463 5610 6021 4791
Size: 398 274 147 411 5128
Fragments arranged by size:
5128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3219 3249 1178
Size: 30 165 30 1816 30 4287
Fragments arranged by size:
4287 1816 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2969 3243 1186
Size: 1270 513 274 4301
Fragments arranged by size:
4301 1270 513 274
BseYI C'CCAG_C
Cuts at: 2352 2580 3298 5561 6057 2352
Size: 228 718 2263 496 2653
Fragments arranged by size:
2653 2263 718 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5785
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2227 3808 4569 5597 1540
Size: 275 186 226 1581 761 1028 2301
Fragments arranged by size:
2301 1581 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2466 2714 3357 4419 4504 5575 2148
Size: 318 248 643 1062 85 1071 2931
Fragments arranged by size:
2931 1071 1062 643 318 248 85
BsmI GAATG_Cn'
Cuts at: 3468 3653 3869 3962 3468
Size: 185 216 93 5864
Fragments arranged by size:
5864 216 185 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2148 2238 2433 2466 2714 3357
Size: 399 262 96 90 195 33 248 643
Cuts at: 3357 3677 4419 4504 5575 1391
Size: 320 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 320 262 248
195 96 90 85 33
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3713 844
Size: 2869 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3423 4853 5027 65
Size: 1183 2175 1430 174 1396
Fragments arranged by size:
2175 1430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2256 5002 5189 1700
Size: 110 446 2746 187 2869
Fragments arranged by size:
2869 2746 446 187 110
BsrGI T'GTAC_A
Cuts at: 96 2539 96
Size: 2443 3915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 4418 5430 4418
Size: 1012 5346
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6358
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 3289 2638
Size: 651 5707
BstYI r'GATC_y
Cuts at: 1972 2625 3590 3688 4055 4440 4457 5909 5995
Size: 653 965 98 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 965 653 385 367 346 98
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2532 3399 513
Size: 552 1467 867 3472
Fragments arranged by size:
3472 1467 867 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 4591
Fragments arranged by size:
4591 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2940 3345 3874 4668 4688 2940
Size: 405 529 794 20 4610
Fragments arranged by size:
4610 794 529 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3034 5365 809
Size: 507 1718 2331 1802
Fragments arranged by size:
2331 1802 1718 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 3805 4694
Size: 54 1425 50 275 18 394 1581 889
Cuts at: 4694 6323 8
Size: 1629 43
Fragments arranged by size:
1629 1581 1425 889 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2224 3805 1537
Size: 275 412 1581 4090
Fragments arranged by size:
4090 1581 412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3248 3552 4288 1353
Size: 1895 304 736 3423
Fragments arranged by size:
3423 1895 736 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2788 4945 5319 5465 387
Size: 1064 138 1199 2157 374 146 1280
Fragments arranged by size:
2157 1280 1199 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3355 3355
Size: 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2348 2536 2904 2990 3391 4421
Size: 393 683 33 188 368 86 401 1030
Cuts at: 4421 5018 5805 6073 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1030 787 683 597 401 393 368
268 188 86 33
EcoO109I rG'GnC_Cy
Cuts at: 2048 2049 2136 2048
Size: 1 87 6270
Fragments arranged by size:
6270 87 1
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
EcoRV GAT'ATC
Cuts at: 2859 2890 2859
Size: 31 6327
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 2062 2085 2187 2288 2792 5505 1678
Size: 384 23 102 101 504 2713 2531
Fragments arranged by size:
2713 2531 504 384 102 101 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3219 3251 4999 5031 5073
Size: 32 135 32 1647 32 1748 32 42
Cuts at: 5073 5105 1373
Size: 32 2626
Fragments arranged by size:
2626 1748 1647 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2982 748
Size: 2234 4124
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3260 3683 3260
Size: 423 5935
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 5472 5656 1305
Size: 1350 2817 184 2007
Fragments arranged by size:
2817 2007 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4536
Fragments arranged by size:
4536 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 3108 3383 3479 4316 4675 4834
Size: 648 1221 721 275 96 837 359 159
Cuts at: 4834 518
Size: 2042
Fragments arranged by size:
2042 1221 837 721 648 359 275 159
96
MspA1I CmG'CkG
Cuts at: 1606 2151 2209 2244 2326 2491 2580 2821 3774
Size: 545 58 35 82 165 89 241 953
Cuts at: 3774 4451 5599 5844 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 545 245 241 165
89 82 58 35
NcoI C'CATG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 4339
Fragments arranged by size:
4339 1467 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 3716 5261 1132
Size: 97 2487 1545 2229
Fragments arranged by size:
2487 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2580 1108
Size: 1472 4886
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2136
Size: 6358
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PspOMI G'GGCC_C
Cuts at: 2048 2048
Size: 6358
PstI C_TGCA'G
Cuts at: 830 2249 2565 3214 3710 830
Size: 1419 316 649 496 3478
Fragments arranged by size:
3478 1419 649 496 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
PvuII CAG'CTG
Cuts at: 2244 2326 2491 2580 2244
Size: 82 165 89 6022
Fragments arranged by size:
6022 165 89 82
SacI G_AGCT'C
Cuts at: 3357 3357
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SanDI GG'GwC_CC
Cuts at: 2136 2136
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 3216 4606 6318 3216
Size: 1390 1712 3256
Fragments arranged by size:
3256 1712 1390
SfcI C'TryA_G
Cuts at: 826 1045 2245 2561 3210 3706 4841 5522 5713
Size: 219 1200 316 649 496 1135 681 191
Cuts at: 5713 826
Size: 1471
Fragments arranged by size:
1471 1200 1135 681 649 496 316 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4462 5363 5625 820
Size: 197 926 2519 901 262 1553
Fragments arranged by size:
2519 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 3016 4282 5
Size: 47 2964 1266 2081
Fragments arranged by size:
2964 2081 1266 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6358
StyI C'CwwG_G
Cuts at: 513 1065 2532 3636 3797 513
Size: 552 1467 1104 161 3074
Fragments arranged by size:
3074 1467 1104 552 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 4385 4402 4555 4740 5185 325
Size: 1255 828 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 828 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2539 3214 4604
Size: 274 80 33 51 1443 562 675 1390
Cuts at: 4604 6316 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1390 675 562 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6318 6350 6318
Size: 32 6326
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2984 3125 3584 4487 2984
Size: 141 459 903 4855
Fragments arranged by size:
4855 903 459 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2371 4166 276
Size: 53 83 186 936 837 1795 2468
Fragments arranged by size:
2468 1795 936 837 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI