Restriction map for
pFN21AB9966
(Circular) (Six-base) MAPSORT of: pFN21AB9966.seq Check: 8961 from: 1 to: 6790
pFN21AB9966 6790 bp
With 182 enzymes: SgfI *
March 30, 2011 18:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4600 278
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 3375 4133 1937
Size: 810 628 758 4594
Fragments arranged by size:
4594 810 758 628
Acc65I G'GTAC_C
Cuts at: 4111 4111
Size: 6790
AclI AA'CG_TT
Cuts at: 1563 4089 4917 5290 1563
Size: 2526 828 373 3063
Fragments arranged by size:
3063 2526 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4008 4853 6237 6505 1632
Size: 2376 845 1384 268 1917
Fragments arranged by size:
2376 1917 1384 845 268
AflII C'TTAA_G
Cuts at: 820 1017 2726 820
Size: 197 1709 4884
Fragments arranged by size:
4884 1709 197
AflIII A'CryG_T
Cuts at: 1203 5689 6666 1203
Size: 4486 977 1327
Fragments arranged by size:
4486 1327 977
AgeI A'CCGG_T
Cuts at: 5621 5621
Size: 6790
AhdI GACnn_n'nnGTC
Cuts at: 2928 2959 5519 2928
Size: 31 2560 4199
Fragments arranged by size:
4199 2560 31
AleI CACnn'nnGTG
Cuts at: 1166 2565 1166
Size: 1399 5391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3572 3746 1184
Size: 2388 174 4228
Fragments arranged by size:
4228 2388 174
AlwNI CAG_nnn'CTG
Cuts at: 4533 6105 4533
Size: 1572 5218
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 2491 3960 4099 4325 4477 6586 1493
Size: 998 1469 139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 998 226 152 139
AseI AT'TA_AT
Cuts at: 160 1032 5344 160
Size: 872 4312 1606
Fragments arranged by size:
4312 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 4115 1870
Size: 73 194 1978 4545
Fragments arranged by size:
4545 1978 194 73
BamHI G'GATC_C
Cuts at: 2691 4120 4487 2691
Size: 1429 367 4994
Fragments arranged by size:
4994 1429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3760 3799 4111 5566 618
Size: 325 217 627 1973 39 312 1455 1842
Fragments arranged by size:
1973 1842 1455 627 325 312 217 39
BanII G_rGCy'C
Cuts at: 1391 2051 4109 1391
Size: 660 2058 4072
Fragments arranged by size:
4072 2058 660
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2958 928
Size: 971 1059 4760
Fragments arranged by size:
4760 1059 971
BbvCI CC'TCA_GC
Cuts at: 2735 2735
Size: 6790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4981 5015 1903
Size: 34 3044 34 3678
Fragments arranged by size:
3678 3044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4682 5892 4682
Size: 1210 5580
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6790
BfrBI ATG'CAT
Cuts at: 3059 3059
Size: 6790
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5401 136
Size: 107 122 71 631 605 3729 1525
Fragments arranged by size:
3729 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2232 6785 2232
Size: 4553 2237
BlpI GC'TnA_GC
Cuts at: 2507 3926 4207 2507
Size: 1419 281 5090
Fragments arranged by size:
5090 1419 281
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4851 6007 1790
Size: 2014 1047 1156 2573
Fragments arranged by size:
2573 2014 1156 1047
BmgBI CAC'GTC
Cuts at: 1318 3034 1318
Size: 1716 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 3327 4521
Size: 416 53 83 399 24 1730 180 1194
Cuts at: 4521 5479 442
Size: 958 1753
Fragments arranged by size:
1753 1730 1194 958 416 399 180 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2958 2958
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2866 5450 1239
Size: 1001 626 2584 2579
Fragments arranged by size:
2584 2579 1001 626
Bpu10I CC'TnA_GC
Cuts at: 2039 2050 2735 3048 2039
Size: 11 685 313 5781
Fragments arranged by size:
5781 685 313 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3779 4915 5780 6078 3779
Size: 1136 865 298 4491
Fragments arranged by size:
4491 1136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2026 5453 882
Size: 617 527 3427 2219
Fragments arranged by size:
3427 2219 617 527
BsaAI yAC'GTr
Cuts at: 493 1204 4581 493
Size: 711 3377 2702
Fragments arranged by size:
3377 2702 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4597 4979 5614 275
Size: 53 83 186 936 3064 382 635 1451
Fragments arranged by size:
3064 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5223 5621 5895 6042 6453 5223
Size: 398 274 147 411 5560
Fragments arranged by size:
5560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6565
Fragments arranged by size:
6565 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3685 1186
Size: 2499 4291
BseYI C'CCAG_C
Cuts at: 2819 5993 6489 2819
Size: 3174 496 3120
Fragments arranged by size:
3174 3120 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3467 1163
Size: 573 1731 4486
Fragments arranged by size:
4486 1731 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4240 5001 6029 1540
Size: 275 186 2239 761 1028 2301
Fragments arranged by size:
2301 2239 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3615 4851 4936 6007 3615
Size: 1236 85 1071 4398
Fragments arranged by size:
4398 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 2033 2149 2570 3059 4301 4394 2033
Size: 116 421 489 1242 93 4429
Fragments arranged by size:
4429 1242 489 421 116 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2842 2842
Size: 6790
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 3615 3804 4109 4851 4936 6007
Size: 399 261 1564 189 305 742 85 1071
Cuts at: 6007 1391
Size: 2174
Fragments arranged by size:
2174 1564 1071 742 399 305 261 189
85
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 4679 6409 4679
Size: 1730 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 3047 3255 4145 844
Size: 1490 713 208 890 3489
Fragments arranged by size:
3489 1490 890 713 208
BsrBI CCG'CTC
Cuts at: 1394 1823 4677 1394
Size: 429 2854 3507
Fragments arranged by size:
3507 2854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2824 3025 3255 5285 5459 65
Size: 1183 1576 201 230 2030 174 1396
Fragments arranged by size:
2030 1576 1396 1183 230 201 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5434 5621 1700
Size: 110 3624 187 2869
Fragments arranged by size:
3624 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6790
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6790
BssSI C'ACGA_G
Cuts at: 4850 5862 4850
Size: 1012 5778
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3427 794
Size: 2633 4157
BstBI TT'CG_AA
Cuts at: 5594 5594
Size: 6790
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6790
BstXI CCAn_nnnn'nTGG
Cuts at: 3433 3472 3724 3433
Size: 39 252 6499
Fragments arranged by size:
6499 252 39
BstYI r'GATC_y
Cuts at: 1972 2067 2232 2691 2936 4120 4487 4872 4889
Size: 95 165 459 245 1184 367 385 17
Cuts at: 4889 6341 6427 6439 6785 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1184 459 385 367 346 245
165 95 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2343 2343
Size: 6790
BtgI C'CryG_G
Cuts at: 513 1065 2004 2647 3561 513
Size: 552 939 643 914 3742
Fragments arranged by size:
3742 939 914 643 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6051
Fragments arranged by size:
6051 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 4015 4306 5100 5120 2074
Size: 1941 291 794 20 3744
Fragments arranged by size:
3744 1941 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6755
DraI TTT'AAA
Cuts at: 4094 4094
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3129 3726 4012
Size: 54 1425 50 275 18 1299 597 286
Cuts at: 4012 4237 5126 6755 8
Size: 225 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 597 286 275 225
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4237 1537
Size: 275 2425 4090
Fragments arranged by size:
4090 2425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 4720 1353
Size: 2674 693 3423
Fragments arranged by size:
3423 2674 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3443 5377 5751 5897 387
Size: 1064 138 1854 1934 374 146 1280
Fragments arranged by size:
1934 1854 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2866 4008 4853 5450 6237 6505
Size: 393 608 626 1142 845 597 787 268
Cuts at: 6505 1239
Size: 1524
Fragments arranged by size:
1524 1142 845 787 626 608 597 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2215 2215
Size: 6790
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
EcoRV GAT'ATC
Cuts at: 3079 3271 3079
Size: 192 6598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2465 2511 2465
Size: 46 6744
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 5937 1678
Size: 4259 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2416 2448 2841
Size: 32 135 32 689 32 123 32 393
Cuts at: 2841 2873 2914 2945 2946 2977 3274 3306 5431
Size: 32 41 31 1 31 297 32 2125
Cuts at: 5431 5463 5505 5537 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2125 689 393 297 135 123 42
41 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 3031 4134 4380 669
Size: 988 281 810 283 1103 246 3079
Fragments arranged by size:
3079 1103 988 810 283 281 246
HindIII A'AGCT_T
Cuts at: 748 2174 2783 748
Size: 1426 609 4755
Fragments arranged by size:
4755 1426 609
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KpnI G_GTAC'C
Cuts at: 4115 4115
Size: 6790
MfeI C'AATT_G
Cuts at: 2481 4389 2481
Size: 1908 4882
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2304 2412 5904 6088 1305
Size: 999 108 3492 184 2007
Fragments arranged by size:
3492 2007 999 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3131 3728 4014 10
Size: 54 1425 343 1299 597 286 2786
Fragments arranged by size:
2786 1425 1299 597 343 286 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 2829 3149 3297 3476 3542 3728
Size: 648 1399 264 320 148 179 66 186
Cuts at: 3728 3812 4748 5107 5266 518
Size: 84 936 359 159 2042
Fragments arranged by size:
2042 1399 936 648 359 320 264 186
179 159 148 84 66
MspA1I CmG'CkG
Cuts at: 1606 3433 3503 3589 4206 4883 6031 6276 1606
Size: 1827 70 86 617 677 1148 245 2120
Fragments arranged by size:
2120 1827 1148 677 617 245 86 70
NcoI C'CATG_G
Cuts at: 513 1065 2004 3561 513
Size: 552 939 1557 3742
Fragments arranged by size:
3742 1557 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6790
NotI GC'GGCC_GC
Cuts at: 4237 4237
Size: 6790
NsiI A_TGCA'T
Cuts at: 3061 3061
Size: 6790
NspI r_CATG'y
Cuts at: 1132 1229 3425 4148 5693 1132
Size: 97 2196 723 1545 2229
Fragments arranged by size:
2229 2196 1545 723 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6790
PciI A'CATG_T
Cuts at: 5689 5689
Size: 6790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3309 4010 1108
Size: 2201 701 3888
Fragments arranged by size:
3888 2201 701
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 6372 6404 4859
Size: 32 1481 32 5245
Fragments arranged by size:
5245 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 6790
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6790
PsiI TTA'TAA
Cuts at: 4360 4360
Size: 6790
PstI C_TGCA'G
Cuts at: 830 4142 830
Size: 3312 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 3433 3503 3589 3433
Size: 70 86 6634
Fragments arranged by size:
6634 86 70
SalI G'TCGA_C
Cuts at: 1936 2746 4132 1936
Size: 810 1386 4594
Fragments arranged by size:
4594 1386 810
SapI GCTCTTCn'nnn_
Cuts at: 4027 4027
Size: 6790
SbfI CC_TGCA'GG
Cuts at: 4142 4142
Size: 6790
ScaI AGT'ACT
Cuts at: 5038 6750 5038
Size: 1712 5078
SfcI C'TryA_G
Cuts at: 826 1045 2968 4138 5273 5954 6145 826
Size: 219 1923 1170 1135 681 191 1471
Fragments arranged by size:
1923 1471 1170 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 4117 1872
Size: 2245 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3794 4894 5795 6057 820
Size: 197 926 783 1068 1100 901 262 1553
Fragments arranged by size:
1553 1100 1068 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6790
SphI G_CATG'C
Cuts at: 1132 3425 4148 1132
Size: 2293 723 3774
Fragments arranged by size:
3774 2293 723
SspI AAT'ATT
Cuts at: 5 52 4714 5
Size: 47 4662 2081
Fragments arranged by size:
4662 2081 47
StuI AGG'CCT
Cuts at: 1517 2348 1517
Size: 831 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 3396 3561 4004 4229 513
Size: 552 939 1392 165 443 225 3074
Fragments arranged by size:
3074 1392 939 552 443 225 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3778 4013 4817 4834 4987 5172 5617
Size: 1255 2198 235 804 17 153 185 445
Cuts at: 5617 325
Size: 1498
Fragments arranged by size:
2198 1498 1255 804 445 235 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5036 6748 96
Size: 274 80 33 51 1443 3059 1712 138
Fragments arranged by size:
3059 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6750 6782 6750
Size: 32 6758
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 2671 4126 2671
Size: 1455 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2654 4007 2654
Size: 1353 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6790
XmaI C'CCGG_G
Cuts at: 1870 4115 1870
Size: 2245 4545
XmnI GAAnn'nnTTC
Cuts at: 4919 4919
Size: 6790
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4598 276
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI