Restriction map for
pFN21AB9960
(Circular) (Six-base) MAPSORT of: pFN21AB9960.seq Check: 9808 from: 1 to: 6772
pFN21AB9960 6772 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 4115 1937
Size: 940 1238 4594
Fragments arranged by size:
4594 1238 940
Acc65I G'GTAC_C
Cuts at: 4093 4093
Size: 6772
AclI AA'CG_TT
Cuts at: 1563 4899 5272 1563
Size: 3336 373 3063
Fragments arranged by size:
3336 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2495 2984 3438 3620 3898 4835 6219
Size: 569 294 489 454 182 278 937 1384
Cuts at: 6219 6487 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 937 569 489 454 294 278
268 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 2517 5671 6648 1203
Size: 1314 3154 977 1327
Fragments arranged by size:
3154 1327 1314 977
AgeI A'CCGG_T
Cuts at: 5603 5603
Size: 6772
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2119 1184
Size: 935 5837
AlwNI CAG_nnn'CTG
Cuts at: 2062 4515 6087 2062
Size: 2453 1572 2747
Fragments arranged by size:
2747 2453 1572
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 2927 3341 3537 4081 4307 4459 6568 1493
Size: 1434 414 196 544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1434 544 414 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 2367 3203 5326 160
Size: 872 1335 836 2123 1606
Fragments arranged by size:
2123 1606 1335 872 836
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 4097 1870
Size: 73 2154 4545
Fragments arranged by size:
4545 2154 73
BamHI G'GATC_C
Cuts at: 4102 4469 4102
Size: 367 6405
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4093 5548 618
Size: 325 217 627 2306 1455 1842
Fragments arranged by size:
2306 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 2613 4091 1391
Size: 1102 120 1478 4072
Fragments arranged by size:
4072 1478 1102 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4963 4997 1903
Size: 34 3026 34 3678
Fragments arranged by size:
3678 3026 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2239 4664 5874 2239
Size: 2425 1210 3137
Fragments arranged by size:
3137 2425 1210
BclI T'GATC_A
Cuts at: 1548 2524 3142 3355 1548
Size: 976 618 213 4965
Fragments arranged by size:
4965 976 618 213
BfrBI ATG'CAT
Cuts at: 2891 2891
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5383 136
Size: 107 122 71 631 605 3711 1525
Fragments arranged by size:
3711 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3286 6767 3286
Size: 3481 3291
BlpI GC'TnA_GC
Cuts at: 4189 4189
Size: 6772
Bme1580I G_kGCm'C
Cuts at: 1790 4833 5989 1790
Size: 3043 1156 2573
Fragments arranged by size:
3043 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6772
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4503 5461 442
Size: 416 53 83 399 24 3086 958 1753
Fragments arranged by size:
3086 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3237 3237
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 3171 5432 1239
Size: 1736 196 2261 2579
Fragments arranged by size:
2579 2261 1736 196
Bpu10I CC'TnA_GC
Cuts at: 3708 3708
Size: 6772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4897 5762 6060 4897
Size: 865 298 5609
Fragments arranged by size:
5609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3147 5435 882
Size: 617 1648 2288 2219
Fragments arranged by size:
2288 2219 1648 617
BsaAI yAC'GTr
Cuts at: 493 1204 3077 4563 493
Size: 711 1873 1486 2702
Fragments arranged by size:
2702 1873 1486 711
BsaBI GATnn'nnATC
Cuts at: 3360 6766 3360
Size: 3406 3366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4579 4961 5596 275
Size: 53 83 186 936 3046 382 635 1451
Fragments arranged by size:
3046 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3326 5205 5603 5877 6024 6435 3326
Size: 1879 398 274 147 411 3663
Fragments arranged by size:
3663 1879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3477 3507 1178
Size: 30 165 30 2074 30 4443
Fragments arranged by size:
4443 2074 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6772
BseYI C'CCAG_C
Cuts at: 2032 3761 5975 6471 2032
Size: 1729 2214 496 2333
Fragments arranged by size:
2333 2214 1729 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4222 4983 6011 1540
Size: 275 186 2221 761 1028 2301
Fragments arranged by size:
2301 2221 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 4833 4918 5989 2493
Size: 2340 85 1071 3276
Fragments arranged by size:
3276 2340 1071 85
BsmI GAATG_Cn'
Cuts at: 4283 4376 4283
Size: 93 6679
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2613 4091 4833 4918 5989 1391
Size: 399 703 120 1478 742 85 1071 2174
Fragments arranged by size:
2174 1478 1071 742 703 399 120 85
BspEI T'CCGG_A
Cuts at: 6435 6435
Size: 6772
BspHI T'CATG_A
Cuts at: 4661 6391 4661
Size: 1730 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4127 844
Size: 3283 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4659 1394
Size: 429 2836 3507
Fragments arranged by size:
3507 2836 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5267 5441 65
Size: 1183 4019 174 1396
Fragments arranged by size:
4019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5416 5603 1700
Size: 110 3606 187 2869
Fragments arranged by size:
3606 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 96
Size: 2742 4030
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6772
BssSI C'ACGA_G
Cuts at: 4832 5844 4832
Size: 1012 5760
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6772
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 2600 1184
Size: 1416 5356
BstXI CCAn_nnnn'nTGG
Cuts at: 3311 3311
Size: 6772
BstYI r'GATC_y
Cuts at: 1972 3286 3594 4102 4469 4854 4871 6323 6409
Size: 1314 308 508 367 385 17 1452 86
Cuts at: 6409 6421 6767 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1314 508 385 367 346 308
86 17 12
BstZ17I GTA'TAC
Cuts at: 2878 2878
Size: 6772
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5281
Fragments arranged by size:
5281 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6033
Fragments arranged by size:
6033 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4045 4288 5082 5102 3583
Size: 462 243 794 20 5253
Fragments arranged by size:
5253 794 462 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 3162 3321 4076 3162
Size: 159 755 5858
Fragments arranged by size:
5858 755 159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5779 809
Size: 507 4463 1802
Fragments arranged by size:
4463 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2352 3510
Size: 54 1425 50 275 18 223 299 1158
Cuts at: 3510 3716 4219 5108 6737 8
Size: 206 503 889 1629 43
Fragments arranged by size:
1629 1425 1158 889 503 299 275 223
206 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4219 1537
Size: 275 2407 4090
Fragments arranged by size:
4090 2407 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2655 3585 4702 1353
Size: 1302 930 1117 3423
Fragments arranged by size:
3423 1302 1117 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5359 5733 5879 387
Size: 1064 138 3770 374 146 1280
Fragments arranged by size:
3770 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 2491
Size: 6772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2495 2975 2984 3171 3438 3620
Size: 393 569 294 480 9 187 267 182
Cuts at: 3620 3898 4835 5432 6219 6487 1239
Size: 278 937 597 787 268 1524
Fragments arranged by size:
1524 937 787 597 569 480 393 294
278 268 267 187 182 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 6772
EcoO109I rG'GnC_Cy
Cuts at: 3229 3442 3934 3229
Size: 213 492 6067
Fragments arranged by size:
6067 492 213
EcoRI G'AATT_C
Cuts at: 4081 4459 4081
Size: 378 6394
EcoRV GAT'ATC
Cuts at: 3667 3667
Size: 6772
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 5919 1678
Size: 4241 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 3579 3611 3656
Size: 32 135 32 1091 32 884 32 45
Cuts at: 3656 3688 5413 5445 5487 5519 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 1091 884 135 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4116 4362 669
Size: 988 281 2178 246 3079
Fragments arranged by size:
3079 2178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KpnI G_GTAC'C
Cuts at: 4097 4097
Size: 6772
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5886 6070 1305
Size: 4581 184 2007
Fragments arranged by size:
4581 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 2354 3512 3718 10
Size: 54 1425 343 223 299 1158 206 3064
Fragments arranged by size:
3064 1425 1158 343 299 223 206 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2871 4730 5089 5248 518
Size: 648 1089 616 1859 359 159 2042
Fragments arranged by size:
2042 1859 1089 648 616 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2103 2255 4188 4865 6013 6258 1606
Size: 456 41 152 1933 677 1148 245 2120
Fragments arranged by size:
2120 1933 1148 677 456 245 152 41
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5281
Fragments arranged by size:
5281 939 552
NdeI CA'TA_TG
Cuts at: 387 3269 387
Size: 2882 3890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NsiI A_TGCA'T
Cuts at: 2893 2893
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2521 4130 5675 1132
Size: 97 1292 1609 1545 2229
Fragments arranged by size:
2229 1609 1545 1292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6772
PciI A'CATG_T
Cuts at: 2517 5671 2517
Size: 3154 3618
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3502 1108
Size: 2394 4378
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3229 3442 3229
Size: 213 6559
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2007 2039 2397 2429 2007
Size: 32 358 32 6350
Fragments arranged by size:
6350 358 32 32
PstI C_TGCA'G
Cuts at: 830 2108 4124 830
Size: 1278 2016 3478
Fragments arranged by size:
3478 2016 1278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
PvuII CAG'CTG
Cuts at: 2103 2255 2103
Size: 152 6620
SacI G_AGCT'C
Cuts at: 2493 2493
Size: 6772
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SbfI CC_TGCA'GG
Cuts at: 4124 4124
Size: 6772
ScaI AGT'ACT
Cuts at: 2435 5020 6732 2435
Size: 2585 1712 2475
Fragments arranged by size:
2585 2475 1712
SfcI C'TryA_G
Cuts at: 826 1045 2104 4120 5255 5936 6127 826
Size: 219 1059 2016 1135 681 191 1471
Fragments arranged by size:
2016 1471 1135 1059 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 4099 1872
Size: 2227 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4876 5777 6039 820
Size: 197 926 2933 901 262 1553
Fragments arranged by size:
2933 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 4130 1132
Size: 2998 3774
SspI AAT'ATT
Cuts at: 5 52 2384 4696 5
Size: 47 2332 2312 2081
Fragments arranged by size:
2332 2312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6772
StyI C'CwwG_G
Cuts at: 513 1065 2004 4211 513
Size: 552 939 2207 3074
Fragments arranged by size:
3074 2207 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3639 3956 4799 4816 4969 5154 5599
Size: 1255 2059 317 843 17 153 185 445
Cuts at: 5599 325
Size: 1498
Fragments arranged by size:
2059 1498 1255 843 445 317 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 2838 3307
Size: 274 80 33 51 1443 456 405 469
Cuts at: 3307 5018 6730 96
Size: 1711 1712 138
Fragments arranged by size:
1712 1711 1443 469 456 405 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2535 2567 2573 2605 6732 6764 2535
Size: 32 6 32 4127 32 2543
Fragments arranged by size:
4127 2543 32 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6772
XbaI T'CTAG_A
Cuts at: 3289 4108 3289
Size: 819 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6772
XmaI C'CCGG_G
Cuts at: 1870 4097 1870
Size: 2227 4545
XmnI GAAnn'nnTTC
Cuts at: 2746 3528 4901 2746
Size: 782 1373 4617
Fragments arranged by size:
4617 1373 782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI