Restriction map for
pFN21AB9959
(Circular) (Six-base) MAPSORT of: pFN21AB9959.seq Check: 4379 from: 1 to: 6319
pFN21AB9959 6319 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2178 2239 2584 2723 3466 2178
Size: 61 345 139 743 5031
Fragments arranged by size:
5031 743 345 139 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2859 4129 278
Size: 53 83 186 936 1323 1270 2468
Fragments arranged by size:
2468 1323 1270 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 2820 3640 2820
Size: 820 5499
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2808 4382 5766 6034 1632
Size: 1176 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1384 1176 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 2678 1166
Size: 1512 4807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 6287
AlwNI CAG_nnn'CTG
Cuts at: 3175 4062 5634 3175
Size: 887 1572 3860
Fragments arranged by size:
3860 1572 887
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2123 2141 2686 3603 3644 1870
Size: 73 67 113 18 545 917 41 4545
Fragments arranged by size:
4545 917 545 113 73 67 41 18
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2680 2820 2906 3332
Size: 325 217 627 360 533 140 86 426
Cuts at: 3332 3442 3640 5095 618
Size: 110 198 1455 1842
Fragments arranged by size:
1842 1455 627 533 426 360 325 217
198 140 110 86
BanII G_rGCy'C
Cuts at: 1391 2098 2364 2394 2877 2939 3638 1391
Size: 707 266 30 483 62 699 4072
Fragments arranged by size:
4072 707 699 483 266 62 30
BbeI G_GCGC'C
Cuts at: 3336 3336
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 3474 928
Size: 971 1305 270 3773
Fragments arranged by size:
3773 1305 971 270
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 4510 4544 1903
Size: 34 98 34 2441 34 3678
Fragments arranged by size:
3678 2441 98 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 3451 4930
Size: 107 122 71 631 605 706 1073 1479
Cuts at: 4930 136
Size: 1525
Fragments arranged by size:
1525 1479 1073 706 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3024 3736 3024
Size: 712 5607
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2685 4380 5536 1790
Size: 360 535 1695 1156 2573
Fragments arranged by size:
2573 1695 1156 535 360
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 2617 4050
Size: 416 53 83 399 24 932 268 1433
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 1433 958 932 416 399 268 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2156 3283 2156
Size: 1127 5192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2911 3224 3269 3432 4979 1239
Size: 1109 563 313 45 163 1547 2579
Fragments arranged by size:
2579 1547 1109 563 313 163 45
Bpu10I CC'TnA_GC
Cuts at: 2502 2846 2502
Size: 344 5975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3293 4444 5309 5607 3293
Size: 1151 865 298 4005
Fragments arranged by size:
4005 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3041 4982 882
Size: 617 1542 1941 2219
Fragments arranged by size:
2219 1941 1542 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2370 2856 3333
Size: 53 83 186 936 515 322 486 477
Cuts at: 3333 4126 4508 5143 275
Size: 793 382 635 1451
Fragments arranged by size:
1451 936 793 635 515 486 477 382
322 186 83 53
BsaWI w'CCGG_w
Cuts at: 2090 4752 5150 5424 5571 5982 2090
Size: 2662 398 274 147 411 2427
Fragments arranged by size:
2662 2427 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 3500 3530 1178
Size: 30 165 30 744 30 1323 30 3967
Fragments arranged by size:
3967 1323 744 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3203 3509 3518 3521 1186
Size: 2017 306 9 3 3984
Fragments arranged by size:
3984 2017 306 9 3
BseYI C'CCAG_C
Cuts at: 2276 2285 2940 3270 5522 6018 2276
Size: 9 655 330 2252 496 2577
Fragments arranged by size:
2577 2252 655 496 330 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2974 1163
Size: 573 1238 4508
Fragments arranged by size:
4508 1238 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3769 4530 5558 1540
Size: 275 186 829 939 761 1028 2301
Fragments arranged by size:
2301 1028 939 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2339 2364 2897 3300 4380 4465 5536 2339
Size: 25 533 403 1080 85 1071 3122
Fragments arranged by size:
3122 1080 1071 533 403 85 25
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 2326 2326
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2150 2339 2364 2394 2685 2877
Size: 399 308 52 189 25 30 291 192
Cuts at: 2877 2897 2939 3300 3638 4380 4465 5536 1391
Size: 20 42 361 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 338 308 291
192 189 85 52 42 30 25 20
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2239 2584 2723 3019 3466 3674 844
Size: 1334 61 345 139 296 447 208 3489
Fragments arranged by size:
3489 1334 447 345 296 208 139 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2308 4206 1394
Size: 429 485 1898 3507
Fragments arranged by size:
3507 1898 485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3111 4814 4988 65
Size: 1183 1863 1703 174 1396
Fragments arranged by size:
1863 1703 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3150 4963 5150 1700
Size: 110 1340 1813 187 2869
Fragments arranged by size:
2869 1813 1340 187 110
BsrGI T'GTAC_A
Cuts at: 96 2193 96
Size: 2097 4222
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 3355 794
Size: 2381 180 3758
Fragments arranged by size:
3758 2381 180
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 2206 2710 1184
Size: 1022 504 4793
Fragments arranged by size:
4793 1022 504
BstXI CCAn_nnnn'nTGG
Cuts at: 3007 3424 3616 3007
Size: 417 192 5710
Fragments arranged by size:
5710 417 192
BstYI r'GATC_y
Cuts at: 1972 2583 3481 3649 4016 4401 4418 5870 5956
Size: 611 898 168 367 385 17 1452 86
Cuts at: 5956 5968 6314 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 898 611 385 367 346 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2902 2902
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2386 2877 3006 3132 513
Size: 552 939 147 235 491 129 126 3700
Fragments arranged by size:
3700 939 552 491 235 147 129 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3085 487
Size: 683 56 1859 3721
Fragments arranged by size:
3721 1859 683 56
BtsI GCAGTG_nn'
Cuts at: 2931 2964 3835 4629 4649 2931
Size: 33 871 794 20 4601
Fragments arranged by size:
4601 871 794 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2959 2959
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2629 5326 809
Size: 507 1313 2697 1802
Fragments arranged by size:
2697 1802 1313 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2250 2643
Size: 54 1425 50 275 18 230 190 393
Cuts at: 2643 2827 3766 4655 6284 8
Size: 184 939 889 1629 43
Fragments arranged by size:
1629 1425 939 889 393 275 230 190
184 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 3766 1537
Size: 275 1015 939 4090
Fragments arranged by size:
4090 1015 939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3281 3507 4249 1353
Size: 1928 226 742 3423
Fragments arranged by size:
3423 1928 742 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 6319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2808 2911 3224 3269 3432 4382
Size: 393 716 460 103 313 45 163 950
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 950 787 716 597 460 393 313
268 163 103 45
EcoO109I rG'GnC_Cy
Cuts at: 2273 2530 2637 3209 3288 3398 3429 2273
Size: 257 107 572 79 110 31 5163
Fragments arranged by size:
5163 572 257 110 107 79 31
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2221 3336 5466 1678
Size: 543 1115 2130 2531
Fragments arranged by size:
2531 2130 1115 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2297 2329 3536
Size: 32 135 32 609 32 84 32 1207
Cuts at: 3536 3568 4960 4992 5034 5066 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1207 609 135 84 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3332 3332
Size: 6319
KpnI G_GTAC'C
Cuts at: 2824 3644 2824
Size: 820 5499
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2244 2678 3440 3614 4277 4636 4795
Size: 648 1078 434 762 174 663 359 159
Cuts at: 4795 518
Size: 2042
Fragments arranged by size:
2042 1078 762 663 648 434 359 174
159
MspA1I CmG'CkG
Cuts at: 1606 2018 2053 2065 2944 3160 3735 4412 5560
Size: 412 35 12 879 216 575 677 1148
Cuts at: 5560 5805 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 879 677 575 412 245 216
35 12
NaeI GCC'GGC
Cuts at: 3152 3152
Size: 6319
NarI GG'CG_CC
Cuts at: 3333 3333
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 3150 3150
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3677 5222 1132
Size: 97 2448 1545 2229
Fragments arranged by size:
2448 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2932 1348
Size: 1584 4735
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 2959 1108
Size: 1140 711 4468
Fragments arranged by size:
4468 1140 711
PfoI T'CCnGG_A
Cuts at: 2082 2185 2364 2082
Size: 103 179 6037
Fragments arranged by size:
6037 179 103
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2273 2530 2637 3209 3398 3429 2273
Size: 257 107 572 189 31 5163
Fragments arranged by size:
5163 572 257 189 107 31
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PstI C_TGCA'G
Cuts at: 830 3180 3671 830
Size: 2350 491 3478
Fragments arranged by size:
3478 2350 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2944 2944
Size: 6319
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 2273 2637 3429 2273
Size: 364 792 5163
Fragments arranged by size:
5163 792 364
SapI GCTCTTCn'nnn_
Cuts at: 3281 3281
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 3176 3556 3667 4802 5483 5674 826
Size: 219 2131 380 111 1135 681 191 1471
Fragments arranged by size:
2131 1471 1135 681 380 219 191 111
SfoI GGC'GCC
Cuts at: 3334 3334
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2012 3605 3646 1872
Size: 140 1593 41 4545
Fragments arranged by size:
4545 1593 140 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3308 4423 5324 5586 820
Size: 197 926 1365 1115 901 262 1553
Fragments arranged by size:
1553 1365 1115 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SrfI GCCC'GGGC
Cuts at: 2012 2012
Size: 6319
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 2676 3381 3758 513
Size: 552 939 438 234 705 377 3074
Fragments arranged by size:
3074 939 705 552 438 377 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 4565 6277
Size: 274 80 33 51 1443 216 2372 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
2372 1712 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 6279 6311 2147
Size: 32 4100 32 2155
Fragments arranged by size:
4100 2155 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3484 3655 3484
Size: 171 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2010 3603 3644 1870
Size: 140 1593 41 4545
Fragments arranged by size:
4545 1593 140 41
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2857 4127 276
Size: 53 83 186 936 1323 1270 2468
Fragments arranged by size:
2468 1323 1270 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SwaI XcmI