Restriction map for
pFN21AB9957
(Circular) (Six-base) MAPSORT of: pFN21AB9957.seq Check: 353 from: 1 to: 6319
pFN21AB9957 6319 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3320 3391 3320
Size: 71 6248
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2959 3169 3662 1937
Size: 1022 210 493 4594
Fragments arranged by size:
4594 1022 493 210
Acc65I G'GTAC_C
Cuts at: 3262 3640 3262
Size: 378 5941
AclI AA'CG_TT
Cuts at: 1563 3112 4446 4819 1563
Size: 1549 1334 373 3063
Fragments arranged by size:
3063 1549 1334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2199 2373 2429 3047 4382 5766 6034
Size: 516 51 174 56 618 1335 1384 268
Cuts at: 6034 1632
Size: 1917
Fragments arranged by size:
1917 1384 1335 618 516 268 174 56
51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 2106 5150 2106
Size: 3044 3275
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3217 1184
Size: 2033 4286
AlwNI CAG_nnn'CTG
Cuts at: 2035 3191 4062 5634 2035
Size: 1156 871 1572 2720
Fragments arranged by size:
2720 1572 1156 871
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2896 3048 3598 3628 3854 4006 6115 1493
Size: 1403 152 550 30 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 550 226 152 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 1870
Size: 73 1701 4545
Fragments arranged by size:
4545 1701 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3033 3262 3640 5095 618
Size: 325 217 627 1246 229 378 1455 1842
Fragments arranged by size:
1842 1455 1246 627 378 325 229 217
BanII G_rGCy'C
Cuts at: 1391 3367 3583 3638 1391
Size: 1976 216 55 4072
Fragments arranged by size:
4072 1976 216 55
BbeI G_GCGC'C
Cuts at: 3037 3037
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3377 3385 928
Size: 971 1478 8 3862
Fragments arranged by size:
3862 1478 971 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2404 2545 6314 2404
Size: 141 3769 2409
Fragments arranged by size:
3769 2409 141
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2446 4380 5536 1790
Size: 656 1934 1156 2573
Fragments arranged by size:
2573 1934 1156 656
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 4050 5008
Size: 416 53 83 399 24 882 1751 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
1753 1751 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 3447 3597 4979 1239
Size: 1472 736 150 1382 2579
Fragments arranged by size:
2579 1472 1382 736 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2541 3017 3410 4982 882
Size: 617 1042 476 393 1572 2219
Fragments arranged by size:
2219 1572 1042 617 476 393
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3034 4126 4508 5143
Size: 53 83 186 936 1501 1092 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1501 1451 1092 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2106 2448 4752 5150 5424 5571 5982 2106
Size: 342 2304 398 274 147 411 2443
Fragments arranged by size:
2443 2304 411 398 342 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6319
BseYI C'CCAG_C
Cuts at: 3325 5522 6018 3325
Size: 2197 496 3626
Fragments arranged by size:
3626 2197 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5746
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2312 3583 4380 4465 5536 2312
Size: 1271 797 85 1071 3095
Fragments arranged by size:
3095 1271 1071 797 85
BsmI GAATG_Cn'
Cuts at: 2364 3830 3923 2364
Size: 1466 93 4760
Fragments arranged by size:
4760 1466 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2446 3367 3583 3638 4380 4465
Size: 399 522 134 921 216 55 742 85
Cuts at: 4465 5536 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 921 742 522 399 216 134
85 55
BspEI T'CCGG_A
Cuts at: 2448 5982 2448
Size: 3534 2785
BspHI T'CATG_A
Cuts at: 2632 4208 5938 2632
Size: 1576 1730 3013
Fragments arranged by size:
3013 1730 1576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 3391 3674 844
Size: 2476 71 283 3489
Fragments arranged by size:
3489 2476 283 71
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3204 3525 4814 4988 65
Size: 1183 1956 321 1289 174 1396
Fragments arranged by size:
1956 1396 1289 1183 321 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 4963 5150 1700
Size: 110 296 2857 187 2869
Fragments arranged by size:
2869 2857 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3065 794
Size: 2271 4048
BstBI TT'CG_AA
Cuts at: 3595 5123 3595
Size: 1528 4791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 3360 3360
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2404 2545 3321 3649 4016 4401 4418 5870
Size: 432 141 776 328 367 385 17 1452
Cuts at: 5870 5956 5968 6314 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 776 432 385 367 346 328
141 86 17 12
BstZ17I GTA'TAC
Cuts at: 2960 3170 2960
Size: 210 6109
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 2341 3623 2341
Size: 1282 5037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284
Size: 54 1425 50 275 18 1936 889 1629
Cuts at: 6284 8
Size: 43
Fragments arranged by size:
1936 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3354 4249 1353
Size: 2001 895 3423
Fragments arranged by size:
3423 2001 895
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3581 3581
Size: 6319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2199 2373 2429 2711 3047 3447
Size: 393 516 51 174 56 282 336 400
Cuts at: 3447 3597 4382 4979 5766 6034 1239
Size: 150 785 597 787 268 1524
Fragments arranged by size:
1524 787 785 597 516 400 393 336
282 268 174 150 56 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 2450
Size: 6319
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 3037 5466 1678
Size: 1359 2429 2531
Fragments arranged by size:
2531 2429 1359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3349 3381 3445 3477 4960
Size: 32 135 32 1777 32 64 32 1483
Cuts at: 4960 4992 5034 5066 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1777 1483 135 64 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2181 748
Size: 1433 4886
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3033 3033
Size: 6319
KpnI G_GTAC'C
Cuts at: 3266 3644 3266
Size: 378 5941
MfeI C'AATT_G
Cuts at: 2592 3918 2592
Size: 1326 4993
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 3534 5433 5617 1305
Size: 1032 1197 1899 184 2007
Fragments arranged by size:
2007 1899 1197 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 4277 4636 4795 518
Size: 648 924 2187 359 159 2042
Fragments arranged by size:
2187 2042 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3034 3034
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2434 3553 3677 5222 1132
Size: 97 1205 1119 124 1545 2229
Fragments arranged by size:
2229 1545 1205 1119 124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3332 1108
Size: 2224 4095
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3078 3889 3078
Size: 811 5508
PstI C_TGCA'G
Cuts at: 830 3196 3671 830
Size: 2366 475 3478
Fragments arranged by size:
3478 2366 475
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SacI G_AGCT'C
Cuts at: 3583 3583
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 2883 4567 6279 2883
Size: 1684 1712 2923
Fragments arranged by size:
2923 1712 1684
SfcI C'TryA_G
Cuts at: 826 1045 2457 2697 3192 3279 3667 4802 5483
Size: 219 1412 240 495 87 388 1135 681
Cuts at: 5483 5674 826
Size: 191 1471
Fragments arranged by size:
1471 1412 1135 681 495 388 240 219
191 87
SfoI GGC'GCC
Cuts at: 3035 3035
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4423 5324 5586 820
Size: 197 926 2480 901 262 1553
Fragments arranged by size:
2480 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 3758 513
Size: 552 939 1754 3074
Fragments arranged by size:
3074 1754 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2881 3182 4565
Size: 274 80 33 51 1443 904 301 1383
Cuts at: 4565 6277 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1383 904 301 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 6279 6311 2205
Size: 32 4042 32 2213
Fragments arranged by size:
4042 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 2736 4448 2736
Size: 1712 4607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI