Restriction map for
pFN21AB9956
(Circular) (Six-base) MAPSORT of: pFN21AB9956.seq Check: 7211 from: 1 to: 6757
pFN21AB9956 6757 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4100 1937
Size: 2163 4594
Acc65I G'GTAC_C
Cuts at: 4078 4078
Size: 6757
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2400 2583 3042 3056 3653 3741 4820 6204
Size: 768 183 459 14 597 88 1079 1384
Cuts at: 6204 6472 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1079 768 597 459 268 183
88 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 3213 5656 6633 1203
Size: 2010 2443 977 1327
Fragments arranged by size:
2443 2010 1327 977
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 3333 1166
Size: 2167 4590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 4500 6072 4500
Size: 1572 5185
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 2023 2518 2534 3733 3788 3862 4026 4066
Size: 530 495 16 1199 55 74 164 40
Cuts at: 4066 4292 4444 6553 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 530 495 226 164 152
74 55 40 16
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 4082 1870
Size: 73 2139 4545
Fragments arranged by size:
4545 2139 73
AvrII C'CTAG_G
Cuts at: 2809 2809
Size: 6757
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 4078 5533 618
Size: 325 217 627 353 1938 1455 1842
Fragments arranged by size:
1938 1842 1455 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2303 3279 4076 1391
Size: 654 258 976 797 4072
Fragments arranged by size:
4072 976 797 654 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5786
BbvCI CC'TCA_GC
Cuts at: 3933 3933
Size: 6757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4948 4982 1903
Size: 34 3011 34 3678
Fragments arranged by size:
3678 3011 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3150 3271 4649 5859 3150
Size: 121 1378 1210 4048
Fragments arranged by size:
4048 1378 1210 121
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6757
BfrBI ATG'CAT
Cuts at: 2920 3437 2920
Size: 517 6240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2958 5368 136
Size: 107 122 71 631 605 1286 2410 1525
Fragments arranged by size:
2410 1525 1286 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 4174 4174
Size: 6757
Bme1580I G_kGCm'C
Cuts at: 1790 4818 5974 1790
Size: 3028 1156 2573
Fragments arranged by size:
3028 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 2636 2758
Size: 416 53 83 399 24 833 386 122
Cuts at: 2758 2833 2965 3259 4488 5446 442
Size: 75 132 294 1229 958 1753
Fragments arranged by size:
1753 1229 958 833 416 399 386 294
132 122 83 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 2528 2900 5417 1239
Size: 761 528 372 2517 2579
Fragments arranged by size:
2579 2517 761 528 372
Bpu10I CC'TnA_GC
Cuts at: 3933 3933
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3398 4882 5747 6045 3398
Size: 1484 865 298 4110
Fragments arranged by size:
4110 1484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5420 882
Size: 617 3921 2219
Fragments arranged by size:
3921 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 6751 6751
Size: 6757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4564 4946 5581 275
Size: 53 83 186 936 3031 382 635 1451
Fragments arranged by size:
3031 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5190 5588 5862 6009 6420 5190
Size: 398 274 147 411 5527
Fragments arranged by size:
5527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2193 2223 1178
Size: 30 165 30 790 30 5712
Fragments arranged by size:
5712 790 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2499 1186
Size: 1313 5444
BseYI C'CCAG_C
Cuts at: 2086 2305 3197 5960 6456 2086
Size: 219 892 2763 496 2387
Fragments arranged by size:
2763 2387 892 496 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 4968 5996 1540
Size: 275 186 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2303 3279 4818 4903 5974 2303
Size: 976 1539 85 1071 3086
Fragments arranged by size:
3086 1539 1071 976 85
BsmI GAATG_Cn'
Cuts at: 2335 2810 2920 4268 4361 2335
Size: 475 110 1348 93 4731
Fragments arranged by size:
4731 1348 475 110 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2303 3279 4076 4818 4903 5974
Size: 399 255 258 976 797 742 85 1071
Cuts at: 5974 1391
Size: 2174
Fragments arranged by size:
2174 1071 976 797 742 399 258 255
85
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 4646 6376 4646
Size: 1730 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4112 844
Size: 3268 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4644 1394
Size: 429 2821 3507
Fragments arranged by size:
3507 2821 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2402 2980 5252 5426 65
Size: 1183 1154 578 2272 174 1396
Fragments arranged by size:
2272 1396 1183 1154 578 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5401 5588 1700
Size: 110 3591 187 2869
Fragments arranged by size:
3591 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 96
Size: 2478 4279
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 4817 5829 4817
Size: 1012 5745
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2327 794
Size: 1533 5224
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 3161 1184
Size: 1977 4780
BstYI r'GATC_y
Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752
Size: 2115 367 385 17 1452 86 12 346
Cuts at: 6752 1972
Size: 1977
Fragments arranged by size:
2115 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2385 2385
Size: 6757
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5266
Fragments arranged by size:
5266 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 4273 5067 5087 2788
Size: 1485 794 20 4458
Fragments arranged by size:
4458 1485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 2250 3402 3621 4061 2250
Size: 1152 219 440 4946
Fragments arranged by size:
4946 1152 440 219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2708 4204 5093
Size: 54 1425 50 275 18 878 1496 889
Cuts at: 5093 6722 8
Size: 1629 43
Fragments arranged by size:
1629 1496 1425 889 878 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 4687 1353
Size: 1026 2308 3423
Fragments arranged by size:
3423 2308 1026
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5344 5718 5864 387
Size: 1064 138 3755 374 146 1280
Fragments arranged by size:
3755 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2301 3277 2301
Size: 976 5781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2400 2528 2583 2900 3042 3056
Size: 393 368 400 128 55 317 142 14
Cuts at: 3056 3653 3741 4820 5417 6204 6472 1239
Size: 597 88 1079 597 787 268 1524
Fragments arranged by size:
1524 1079 787 597 597 400 393 368
317 268 142 128 88 55 14
EcoRI G'AATT_C
Cuts at: 4066 4444 4066
Size: 378 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2613 2179
Size: 434 6323
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 5904 1678
Size: 4226 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2908 2940 3098 3130 3737
Size: 32 135 32 1336 32 158 32 607
Cuts at: 3737 3769 5398 5430 5472 5504 1373
Size: 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1629 1336 607 158 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4101 4347 669
Size: 988 281 2163 246 3079
Fragments arranged by size:
3079 2163 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6757
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 4082 4082
Size: 6757
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 2461 5871 6055 1305
Size: 1125 31 3410 184 2007
Fragments arranged by size:
3410 2007 1125 184 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4935
Fragments arranged by size:
4935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 2166 3333 4715 5074 5233 518
Size: 648 952 48 1167 1382 359 159 2042
Fragments arranged by size:
2042 1382 1167 952 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 4173 4850 5998 6243 1606
Size: 2567 677 1148 245 2120
Fragments arranged by size:
2567 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5266
Fragments arranged by size:
5266 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6757
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NsiI A_TGCA'T
Cuts at: 2922 3439 2922
Size: 517 6240
NspI r_CATG'y
Cuts at: 1132 1229 2210 2353 3217 3773 4115 5660 1132
Size: 97 981 143 864 556 342 1545 2229
Fragments arranged by size:
2229 1545 981 864 556 342 143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 3213 5656 3213
Size: 2443 4314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2657 1108
Size: 1549 5208
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2739 4826 4858 6339 6371 2707
Size: 32 2087 32 1481 32 3093
Fragments arranged by size:
3093 2087 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 3149 4327 3149
Size: 1178 5579
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 6725
PstI C_TGCA'G
Cuts at: 830 4109 830
Size: 3279 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
SacI G_AGCT'C
Cuts at: 2303 3279 2303
Size: 976 5781
SalI G'TCGA_C
Cuts at: 1936 4099 1936
Size: 2163 4594
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SfcI C'TryA_G
Cuts at: 826 1045 4105 5240 5921 6112 826
Size: 219 3060 1135 681 191 1471
Fragments arranged by size:
3060 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 4084 1872
Size: 2212 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3377 4861 5762 6024 820
Size: 197 926 1434 1484 901 262 1553
Fragments arranged by size:
1553 1484 1434 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 4115 1132
Size: 2983 3774
SspI AAT'ATT
Cuts at: 5 52 4681 5
Size: 47 4629 2081
Fragments arranged by size:
4629 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6757
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2809 4196 513
Size: 552 939 27 778 1387 3074
Fragments arranged by size:
3074 1387 939 778 552 27
SwaI ATTT'AAAT
Cuts at: 2250 2250
Size: 6757
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 4784 4801 4954 5139 5584 325
Size: 1255 717 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2222 2574 2594
Size: 274 80 33 51 1443 245 352 20
Cuts at: 2594 5003 6715 96
Size: 2409 1712 138
Fragments arranged by size:
2409 1712 1443 352 274 245 138 80
51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6717 6749 6717
Size: 32 6725
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6757
XmaI C'CCGG_G
Cuts at: 1870 4082 1870
Size: 2212 4545
XmnI GAAnn'nnTTC
Cuts at: 4886 4886
Size: 6757
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI XcmI