Restriction map for
pFN21AB9950
(Circular) (Six-base) MAPSORT of: pFN21AB9950.seq Check: 8619 from: 1 to: 6730
pFN21AB9950 6730 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 6730
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4540 278
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4073 1937
Size: 2136 4594
Acc65I G'GTAC_C
Cuts at: 4051 4051
Size: 6730
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2537 2880 3893 4793 6177 6445 1632
Size: 905 343 1013 900 1384 268 1917
Fragments arranged by size:
1917 1384 1013 905 900 343 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6533
AflIII A'CryG_T
Cuts at: 1203 5629 6606 1203
Size: 4426 977 1327
Fragments arranged by size:
4426 1327 977
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 3211 5459 3211
Size: 2248 4482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3081 1184
Size: 1897 4833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 3709 3741 2375
Size: 32 1302 32 5364
Fragments arranged by size:
5364 1302 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2537 3948 4473 6045 2537
Size: 1411 525 1572 3222
Fragments arranged by size:
3222 1572 1411 525
ApaLI G'TGCA_C
Cuts at: 4787 5943 4787
Size: 1156 5574
ApoI r'AATT_y
Cuts at: 1493 2837 2971 3371 3425 4039 4265 4417 6526
Size: 1344 134 400 54 614 226 152 2109
Cuts at: 6526 1493
Size: 1697
Fragments arranged by size:
2109 1697 1344 614 400 226 152 134
54
AseI AT'TA_AT
Cuts at: 160 1032 5284 160
Size: 872 4252 1606
Fragments arranged by size:
4252 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 2526 4055 1870
Size: 73 264 319 1529 4545
Fragments arranged by size:
4545 1529 319 264 73
BamHI G'GATC_C
Cuts at: 2162 3801 4060 4427 2162
Size: 1639 259 367 4465
Fragments arranged by size:
4465 1639 367 259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4051 5506 618
Size: 325 217 627 2264 1455 1842
Fragments arranged by size:
2264 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3356 4049 1391
Size: 1965 693 4072
Fragments arranged by size:
4072 1965 693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2724 3987 928
Size: 971 825 1263 3671
Fragments arranged by size:
3671 1263 971 825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4921 4955 1903
Size: 34 2984 34 3678
Fragments arranged by size:
3678 2984 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 4622 5832 2002
Size: 2620 1210 2900
Fragments arranged by size:
2900 2620 1210
BclI T'GATC_A
Cuts at: 1548 3156 3342 1548
Size: 1608 186 4936
Fragments arranged by size:
4936 1608 186
BfrBI ATG'CAT
Cuts at: 2282 2282
Size: 6730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5341 136
Size: 107 122 71 631 605 3669 1525
Fragments arranged by size:
3669 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3643 6725 3643
Size: 3082 3648
BlpI GC'TnA_GC
Cuts at: 4147 4147
Size: 6730
Bme1580I G_kGCm'C
Cuts at: 1790 4791 5947 1790
Size: 3001 1156 2573
Fragments arranged by size:
3001 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6730
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4461 5419 442
Size: 416 53 83 399 24 3044 958 1753
Fragments arranged by size:
3044 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2893 3729 5390 1239
Size: 1654 836 1661 2579
Fragments arranged by size:
2579 1661 1654 836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4855 5720 6018 4855
Size: 865 298 5567
Fragments arranged by size:
5567 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3883 5393 882
Size: 617 2384 1510 2219
Fragments arranged by size:
2384 2219 1510 617
BsaAI yAC'GTr
Cuts at: 493 1204 3055 4521 493
Size: 711 1851 1466 2702
Fragments arranged by size:
2702 1851 1466 711
BsaBI GATnn'nnATC
Cuts at: 2917 6724 2917
Size: 3807 2923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2219 4537 4919 5554
Size: 53 83 186 936 686 2318 382 635
Cuts at: 5554 275
Size: 1451
Fragments arranged by size:
2318 1451 936 686 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5163 5561 5835 5982 6393 5163
Size: 398 274 147 411 5500
Fragments arranged by size:
5500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 1178
Size: 30 165 30 1194 30 5281
Fragments arranged by size:
5281 1194 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 3523 1186
Size: 1039 1298 4393
Fragments arranged by size:
4393 1298 1039
BseYI C'CCAG_C
Cuts at: 2616 2682 3271 3301 3537 5933 6429 2616
Size: 66 589 30 236 2396 496 2917
Fragments arranged by size:
2917 2396 589 496 236 66 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4180 4941 5969 1540
Size: 275 186 2179 761 1028 2301
Fragments arranged by size:
2301 2179 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4791 4876 5947 4791
Size: 85 1071 5574
Fragments arranged by size:
5574 1071 85
BsmI GAATG_Cn'
Cuts at: 4241 4334 4241
Size: 93 6637
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3356 4049 4791 4876 5947 1391
Size: 399 1566 693 742 85 1071 2174
Fragments arranged by size:
2174 1566 1071 742 693 399 85
BspEI T'CCGG_A
Cuts at: 6393 6393
Size: 6730
BspHI T'CATG_A
Cuts at: 4619 6349 4619
Size: 1730 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 4085 844
Size: 1514 1727 3489
Fragments arranged by size:
3489 1727 1514
BsrBI CCG'CTC
Cuts at: 1394 1823 4617 1394
Size: 429 2794 3507
Fragments arranged by size:
3507 2794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3490 5225 5399 65
Size: 1183 2242 1735 174 1396
Fragments arranged by size:
2242 1735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3900 5374 5561 1700
Size: 110 2090 1474 187 2869
Fragments arranged by size:
2869 2090 1474 187 110
BsrGI T'GTAC_A
Cuts at: 96 2991 3939 96
Size: 2895 948 2887
Fragments arranged by size:
2895 2887 948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6730
BssSI C'ACGA_G
Cuts at: 3953 4790 5802 3953
Size: 837 1012 4881
Fragments arranged by size:
4881 1012 837
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3489 794
Size: 2695 4035
BstBI TT'CG_AA
Cuts at: 5534 5534
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1184 2339 1184
Size: 1155 5575
BstXI CCAn_nnnn'nTGG
Cuts at: 3735 3735
Size: 6730
BstYI r'GATC_y
Cuts at: 1972 2162 3643 3801 4060 4427 4812 4829 6281
Size: 190 1481 158 259 367 385 17 1452
Cuts at: 6281 6367 6379 6725 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1481 1452 385 367 346 259 190
158 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3506 3506
Size: 6730
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5239
Fragments arranged by size:
5239 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5991
Fragments arranged by size:
5991 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 4246 5040 5060 2773
Size: 1473 794 20 4443
Fragments arranged by size:
4443 1473 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6695
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DraIII CAC_nnn'GTG
Cuts at: 3178 3178
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3701 5737 809
Size: 507 2385 2036 1802
Fragments arranged by size:
2385 2036 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4177 5066
Size: 54 1425 50 275 18 1450 897 889
Cuts at: 5066 6695 8
Size: 1629 43
Fragments arranged by size:
1629 1450 1425 897 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4177 1537
Size: 275 2365 4090
Fragments arranged by size:
4090 2365 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2472 2859 3243 4660 1353
Size: 933 186 387 384 1417 3423
Fragments arranged by size:
3423 1417 933 387 384 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3158 5317 5691 5837 387
Size: 1064 138 1569 2159 374 146 1280
Fragments arranged by size:
2159 1569 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2880 2893 3729 3893 4793 5390
Size: 393 905 343 13 836 164 900 597
Cuts at: 5390 6177 6445 1239
Size: 787 268 1524
Fragments arranged by size:
1524 905 900 836 787 597 393 343
268 164 13
EcoO109I rG'GnC_Cy
Cuts at: 3199 3199
Size: 6730
EcoRI G'AATT_C
Cuts at: 2837 3371 4039 4417 2837
Size: 534 668 378 5150
Fragments arranged by size:
5150 668 534 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2051 2108 2282 2051
Size: 57 174 6499
Fragments arranged by size:
6499 174 57
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 5877 1678
Size: 4199 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2287 2319 2421
Size: 32 135 32 428 32 255 32 102
Cuts at: 2421 2453 2543 2575 2579 2611 2645 2677 3197
Size: 32 90 32 4 32 34 32 520
Cuts at: 3197 3229 5371 5403 5445 5477 1373
Size: 32 2142 32 42 32 2626
Fragments arranged by size:
2626 2142 520 428 255 135 102 90
42 34 32 32 32 32 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 4074 4320 669
Size: 988 281 1245 891 246 3079
Fragments arranged by size:
3079 1245 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 3119 748
Size: 2371 4359
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KpnI G_GTAC'C
Cuts at: 4055 4055
Size: 6730
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3694 5844 6028 1305
Size: 2389 2150 184 2007
Fragments arranged by size:
2389 2150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3282 10
Size: 54 1425 343 1450 3458
Fragments arranged by size:
3458 1450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3063 4688 5047 5206 518
Size: 648 1897 1625 359 159 2042
Fragments arranged by size:
2042 1897 1625 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3301 3945 4146 4823 5971 6216 1606
Size: 1695 644 201 677 1148 245 2120
Fragments arranged by size:
2120 1695 1148 677 644 245 201
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5239
Fragments arranged by size:
5239 939 552
NdeI CA'TA_TG
Cuts at: 387 2449 387
Size: 2062 4668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 4177 4177
Size: 6730
NsiI A_TGCA'T
Cuts at: 2284 2284
Size: 6730
NspI r_CATG'y
Cuts at: 1132 1229 2701 4031 4088 5633 1132
Size: 97 1472 1330 57 1545 2229
Fragments arranged by size:
2229 1545 1472 1330 97 57
PasI CC'CwG_GG
Cuts at: 1348 3217 3883 1348
Size: 1869 666 4195
Fragments arranged by size:
4195 1869 666
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 1108
Size: 1245 5485
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PmlI CAC'GTG
Cuts at: 3055 3055
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2022 2054 2916 2948 4799 4831 6312 6344 2022
Size: 32 862 32 1851 32 1481 32 2408
Fragments arranged by size:
2408 1851 1481 862 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3199 3199
Size: 6730
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6730
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 6698
PstI C_TGCA'G
Cuts at: 830 2470 4082 830
Size: 1640 1612 3478
Fragments arranged by size:
3478 1640 1612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 3301 3945 3301
Size: 644 6086
SalI G'TCGA_C
Cuts at: 1936 4072 1936
Size: 2136 4594
SbfI CC_TGCA'GG
Cuts at: 4082 4082
Size: 6730
ScaI AGT'ACT
Cuts at: 4978 6690 4978
Size: 1712 5018
SfcI C'TryA_G
Cuts at: 826 1045 2223 2466 4078 5213 5894 6085 826
Size: 219 1178 243 1612 1135 681 191 1471
Fragments arranged by size:
1612 1471 1178 1135 681 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 4057 1872
Size: 2185 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 4834 5735 5997 820
Size: 197 926 264 2627 901 262 1553
Fragments arranged by size:
2627 1553 926 901 264 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 4654 5
Size: 47 4602 2081
Fragments arranged by size:
4602 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6730
StyI C'CwwG_G
Cuts at: 513 1065 2004 2711 4169 513
Size: 552 939 707 1458 3074
Fragments arranged by size:
3074 1458 939 707 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4757 4774 4927 5112 5557 325
Size: 1255 3177 17 153 185 445 1498
Fragments arranged by size:
3177 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2991 3939 4976
Size: 274 80 33 51 1443 1014 948 1037
Cuts at: 4976 6688 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1037 1014 948 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6690 6722 6690
Size: 32 6698
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6730
XbaI T'CTAG_A
Cuts at: 2023 4066 2023
Size: 2043 4687
XhoI C'TCGA_G
Cuts at: 1943 2207 1943
Size: 264 6466
XmaI C'CCGG_G
Cuts at: 1870 4055 1870
Size: 2185 4545
XmnI GAAnn'nnTTC
Cuts at: 2031 2034 3375 4008 4859 2031
Size: 3 1341 633 851 3902
Fragments arranged by size:
3902 1341 851 633 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4538 276
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI