Restriction map for
pFN21AB9948
(Circular) (Six-base) MAPSORT of: pFN21AB9948.seq Check: 9238 from: 1 to: 6724
pFN21AB9948 6724 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2414 2414
Size: 6724
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2520 4534 278
Size: 53 83 186 936 984 2014 2468
Fragments arranged by size:
2468 2014 984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4067 1937
Size: 2130 4594
Acc65I G'GTAC_C
Cuts at: 4045 4045
Size: 6724
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3143 3789 4787 6171 6439 1632
Size: 1154 357 646 998 1384 268 1917
Fragments arranged by size:
1917 1384 1154 998 646 357 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 6724
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 2325 5623 6600 1203
Size: 1122 3298 977 1327
Fragments arranged by size:
3298 1327 1122 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 5453 5453
Size: 6724
AleI CACnn'nnGTG
Cuts at: 1166 3167 1166
Size: 2001 4723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3089 1184
Size: 1905 4819
AlwNI CAG_nnn'CTG
Cuts at: 2160 4467 6039 2160
Size: 2307 1572 2845
Fragments arranged by size:
2845 2307 1572
ApaLI G'TGCA_C
Cuts at: 2225 2420 3160 4781 5937 2225
Size: 195 740 1621 1156 3012
Fragments arranged by size:
3012 1621 1156 740 195
ApoI r'AATT_y
Cuts at: 1493 2117 2243 2915 2984 3968 3998 4033 4259
Size: 624 126 672 69 984 30 35 226
Cuts at: 4259 4411 6520 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 984 672 624 226 152 126
69 35 30
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 3533 4049 1870
Size: 73 1590 516 4545
Fragments arranged by size:
4545 1590 516 73
BamHI G'GATC_C
Cuts at: 4054 4421 4054
Size: 367 6357
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2235 3619 4045 5500
Size: 325 217 627 288 160 1384 426 1455
Cuts at: 5500 618
Size: 1842
Fragments arranged by size:
1842 1455 1384 627 426 325 288 217
160
BanII G_rGCy'C
Cuts at: 1391 3105 3327 4043 1391
Size: 1714 222 716 4072
Fragments arranged by size:
4072 1714 716 222
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 6724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2642 2725 928
Size: 971 743 83 4927
Fragments arranged by size:
4927 971 743 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3524 3558 4915 4949 1903
Size: 34 1587 34 1357 34 3678
Fragments arranged by size:
3678 1587 1357 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6724
BfrBI ATG'CAT
Cuts at: 3797 3797
Size: 6724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2457 5335 136
Size: 107 122 71 631 605 785 2878 1525
Fragments arranged by size:
2878 1525 785 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6719 6719
Size: 6724
BlpI GC'TnA_GC
Cuts at: 4141 4141
Size: 6724
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2424 3164 4785 5941 1790
Size: 439 195 740 1621 1156 2573
Fragments arranged by size:
2573 1621 1156 740 439 195
BmgBI CAC'GTC
Cuts at: 1318 3154 1318
Size: 1836 4888
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4455 5413 442
Size: 416 53 83 399 24 3038 958 1753
Fragments arranged by size:
3038 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 3501 5384 1239
Size: 1078 1184 1883 2579
Fragments arranged by size:
2579 1883 1184 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4849 5714 6012 4849
Size: 865 298 5561
Fragments arranged by size:
5561 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3000 5387 882
Size: 617 1501 2387 2219
Fragments arranged by size:
2387 2219 1501 617
BsaAI yAC'GTr
Cuts at: 493 1204 2328 2590 3271 4515 493
Size: 711 1124 262 681 1244 2702
Fragments arranged by size:
2702 1244 1124 711 681 262
BsaBI GATnn'nnATC
Cuts at: 6718 6718
Size: 6724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2517 4531 4913
Size: 53 83 186 936 543 441 2014 382
Cuts at: 4913 5548 275
Size: 635 1451
Fragments arranged by size:
2014 1451 936 635 543 441 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3009 3804 5157 5555 5829 5976 6387 3009
Size: 795 1353 398 274 147 411 3346
Fragments arranged by size:
3346 1353 795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2963 2993 1178
Size: 30 165 30 912 30 618 30 4909
Fragments arranged by size:
4909 912 618 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 5850
BseYI C'CCAG_C
Cuts at: 2447 3046 3216 3611 5927 6423 2447
Size: 599 170 395 2316 496 2748
Fragments arranged by size:
2748 2316 599 496 395 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 1163
Size: 573 410 5741
Fragments arranged by size:
5741 573 410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2485 3313 3895 4174 4935 5963
Size: 275 186 484 828 582 279 761 1028
Cuts at: 5963 1540
Size: 2301
Fragments arranged by size:
2301 1028 828 761 582 484 279 275
186
BsiHKAI G_wGCw'C
Cuts at: 2054 2229 2424 3105 3164 4785 4870 5941 2054
Size: 175 195 681 59 1621 85 1071 2837
Fragments arranged by size:
2837 1621 1071 681 195 175 85 59
BsmI GAATG_Cn'
Cuts at: 4235 4328 4235
Size: 93 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2229 2424 3105 3164 3327 4043
Size: 399 264 175 195 681 59 163 716
Cuts at: 4043 4785 4870 5941 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 681 399 264 195
175 163 85 59
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 4613 6343 4613
Size: 1730 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 4079 844
Size: 1570 1665 3489
Fragments arranged by size:
3489 1665 1570
BsrBI CCG'CTC
Cuts at: 1394 1823 4611 1394
Size: 429 2788 3507
Fragments arranged by size:
3507 2788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5219 5393 65
Size: 1183 3971 174 1396
Fragments arranged by size:
3971 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3312 5368 5555 1700
Size: 110 1502 2056 187 2869
Fragments arranged by size:
2869 2056 1502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 4784 5796 4784
Size: 1012 5712
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2160 3353 794
Size: 1366 1193 4165
Fragments arranged by size:
4165 1366 1193
BstBI TT'CG_AA
Cuts at: 3972 5528 3972
Size: 1556 5168
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2319 2581 2774 3117 3840 4054 4421 4806
Size: 347 262 193 343 723 214 367 385
Cuts at: 4806 4823 6275 6361 6373 6719 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 723 385 367 347 346 343
262 214 193 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2081 2081
Size: 6724
BtgI C'CryG_G
Cuts at: 513 1065 2004 2479 3240 3567 513
Size: 552 939 475 761 327 3670
Fragments arranged by size:
3670 939 761 552 475 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3511 487
Size: 683 56 2285 3700
Fragments arranged by size:
3700 2285 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2363 4240 5034 5054 2158
Size: 205 1877 794 20 3828
Fragments arranged by size:
3828 1877 794 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6689
DraI TTT'AAA
Cuts at: 4005 4028 4005
Size: 23 6701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2516 3941 5731 809
Size: 507 1200 1425 1790 1802
Fragments arranged by size:
1802 1790 1425 1200 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 3310 3711
Size: 54 1425 50 275 18 652 828 401
Cuts at: 3711 4171 5060 6689 8
Size: 460 889 1629 43
Fragments arranged by size:
1629 1425 889 828 652 460 401 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2482 3310 4171 1537
Size: 275 670 828 861 4090
Fragments arranged by size:
4090 861 828 670 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3656 4654 1353
Size: 2303 998 3423
Fragments arranged by size:
3423 2303 998
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5311 5685 5831 387
Size: 1064 138 3722 374 146 1280
Fragments arranged by size:
3722 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3103 3103
Size: 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2786 3143 3501 3789 4787 5384
Size: 393 685 469 357 358 288 998 597
Cuts at: 5384 6171 6439 1239
Size: 787 268 1524
Fragments arranged by size:
1524 998 787 685 597 469 393 358
357 288 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3125 3125
Size: 6724
EcoO109I rG'GnC_Cy
Cuts at: 2950 2950
Size: 6724
EcoRI G'AATT_C
Cuts at: 3968 4033 4411 3968
Size: 65 378 6281
Fragments arranged by size:
6281 378 65
EcoRV GAT'ATC
Cuts at: 3784 3784
Size: 6724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 6724
FspI TGC'GCA
Cuts at: 5230 5230
Size: 6724
HaeII r_GCGC'y
Cuts at: 1678 2079 2271 2431 2479 5871 1678
Size: 401 192 160 48 3392 2531
Fragments arranged by size:
3392 2531 401 192 160 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2596 2628 5365 5397 5439
Size: 32 135 32 1024 32 2737 32 42
Cuts at: 5439 5471 1373
Size: 32 2626
Fragments arranged by size:
2737 2626 1024 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3991 4068 4314 669
Size: 988 281 2053 77 246 3079
Fragments arranged by size:
3079 2053 988 281 246 77
HindIII A'AGCT_T
Cuts at: 748 3864 748
Size: 3116 3608
HpaI GTT'AAC
Cuts at: 3991 4314 3991
Size: 323 6401
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 6724
KpnI G_GTAC'C
Cuts at: 4049 4049
Size: 6724
MfeI C'AATT_G
Cuts at: 4323 4323
Size: 6724
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5838 6022 1305
Size: 4533 184 2007
Fragments arranged by size:
4533 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3713 10
Size: 54 1425 343 1881 3021
Fragments arranged by size:
3021 1881 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3167 4682 5041 5200 518
Size: 648 1242 759 1515 359 159 2042
Fragments arranged by size:
2042 1515 1242 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2481 2695 4140 4817 5965 6210 1606
Size: 875 214 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 875 677 245 214
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 6724
NcoI C'CATG_G
Cuts at: 513 1065 2004 3240 513
Size: 552 939 1236 3997
Fragments arranged by size:
3997 1236 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 2482 4171 2482
Size: 1689 5035
NsiI A_TGCA'T
Cuts at: 3799 3799
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 3466 3797 4082 5627 1132
Size: 97 2237 331 285 1545 2229
Fragments arranged by size:
2237 2229 1545 331 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6724
PciI A'CATG_T
Cuts at: 5623 5623
Size: 6724
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3241 1108
Size: 2133 4591
PfoI T'CCnGG_A
Cuts at: 2952 2952
Size: 6724
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4793 4825 6306 6338 4793
Size: 32 1481 32 5179
Fragments arranged by size:
5179 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2950 2950
Size: 6724
PshAI GACnn'nnGTC
Cuts at: 1613 2277 1613
Size: 664 6060
PsiI TTA'TAA
Cuts at: 3855 4294 3855
Size: 439 6285
PstI C_TGCA'G
Cuts at: 830 2156 3349 4076 830
Size: 1326 1193 727 3478
Fragments arranged by size:
3478 1326 1193 727
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2695 2695
Size: 6724
SacI G_AGCT'C
Cuts at: 3105 3105
Size: 6724
SacII CC_GC'GG
Cuts at: 2482 2482
Size: 6724
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SbfI CC_TGCA'GG
Cuts at: 4076 4076
Size: 6724
ScaI AGT'ACT
Cuts at: 2858 4972 6684 2858
Size: 2114 1712 2898
Fragments arranged by size:
2898 2114 1712
SfcI C'TryA_G
Cuts at: 826 1045 2152 3345 4072 5207 5888 6079 826
Size: 219 1107 1193 727 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 1107 727 681 219 191
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 6724
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 4051 1872
Size: 2179 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4828 5729 5991 820
Size: 197 926 2885 901 262 1553
Fragments arranged by size:
2885 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2590 4515 493
Size: 2097 1925 2702
Fragments arranged by size:
2702 2097 1925
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 3797 4082 1132
Size: 2665 285 3774
Fragments arranged by size:
3774 2665 285
SspI AAT'ATT
Cuts at: 5 52 3955 4648 5
Size: 47 3903 693 2081
Fragments arranged by size:
3903 2081 693 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6724
StyI C'CwwG_G
Cuts at: 513 1065 2004 2946 3240 3699 4163 513
Size: 552 939 942 294 459 464 3074
Fragments arranged by size:
3074 942 939 552 464 459 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3038 3350 3756 3909 4751 4768 4921
Size: 1255 1458 312 406 153 842 17 153
Cuts at: 4921 5106 5551 325
Size: 185 445 1498
Fragments arranged by size:
1498 1458 1255 842 445 406 312 185
153 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 3252 4970
Size: 274 80 33 51 1443 879 396 1718
Cuts at: 4970 6682 96
Size: 1712 138
Fragments arranged by size:
1718 1712 1443 879 396 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2383 2415 3895 3927 6684 6716 2315
Size: 32 36 32 1480 32 2757 32 2323
Fragments arranged by size:
2757 2323 1480 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6724
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3706 3706
Size: 6724
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 4049 1870
Size: 2179 4545
XmnI GAAnn'nnTTC
Cuts at: 2765 4853 2765
Size: 2088 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2518 4532 276
Size: 53 83 186 936 984 2014 2468
Fragments arranged by size:
2468 2014 984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI