Restriction map for
pFN21AB9945
(Circular) (Six-base) MAPSORT of: pFN21AB9945.seq Check: 8691 from: 1 to: 6295
pFN21AB9945 6295 bp
With 182 enzymes: SgfI *
April 1, 2011 16:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 3251 3638 1937
Size: 142 1172 387 4594
Fragments arranged by size:
4594 1172 387 142
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 3594 4422 4795 1563
Size: 2031 828 373 3063
Fragments arranged by size:
3063 2031 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 2661 2850 3137 3593 4358 5742 6010
Size: 972 57 189 287 456 765 1384 268
Cuts at: 6010 1632
Size: 1917
Fragments arranged by size:
1917 1384 972 765 456 287 268 189
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 3279 5194 6171 1203
Size: 2076 1915 977 1327
Fragments arranged by size:
2076 1915 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 3317 1166
Size: 2151 4144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 2516 4038 5610 2516
Size: 1522 1572 3201
Fragments arranged by size:
3201 1572 1522
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2276 2791 3085 3604 3830 3982 6091 1493
Size: 783 515 294 519 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 519 515 294 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 3620 1870
Size: 73 130 1547 4545
Fragments arranged by size:
4545 1547 130 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3616 5071 618
Size: 325 217 627 1829 1455 1842
Fragments arranged by size:
1842 1829 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3614 1391
Size: 2223 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3504 928
Size: 971 1605 3719
Fragments arranged by size:
3719 1605 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3060 4187 5397 3060
Size: 1127 1210 3958
Fragments arranged by size:
3958 1210 1127
BclI T'GATC_A
Cuts at: 1548 2845 3309 1548
Size: 1297 464 4534
Fragments arranged by size:
4534 1297 464
BfrBI ATG'CAT
Cuts at: 2315 3123 2315
Size: 808 5487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 4356 5512 1790
Size: 2566 1156 2573
Fragments arranged by size:
2573 2566 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 4955 1239
Size: 807 2909 2579
Fragments arranged by size:
2909 2579 807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3441 4420 5285 5583 3441
Size: 979 865 298 4153
Fragments arranged by size:
4153 979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 4958 882
Size: 617 523 2936 2219
Fragments arranged by size:
2936 2219 617 523
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 2262 3465 6289 2262
Size: 1203 2824 2268
Fragments arranged by size:
2824 2268 1203
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3291 3321 1178
Size: 30 165 30 1888 30 4152
Fragments arranged by size:
4152 1888 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 1186
Size: 1312 4983
BseYI C'CCAG_C
Cuts at: 5498 5994 5498
Size: 496 5799
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5722
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4356 4441 5512 4356
Size: 85 1071 5139
Fragments arranged by size:
5139 1071 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3614 4356 4441 5512 1391
Size: 399 1824 742 85 1071 2174
Fragments arranged by size:
2174 1824 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 2146 4184 5914 2146
Size: 2038 1730 2527
Fragments arranged by size:
2527 2038 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 3650 844
Size: 1716 1090 3489
Fragments arranged by size:
3489 1716 1090
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2398 3499 4790 4964 65
Size: 1183 1150 1101 1291 174 1396
Fragments arranged by size:
1396 1291 1183 1150 1101 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 3446 3625 3992 4377 4394 5846 5932 5944
Size: 1474 179 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1474 1452 385 367 346 179 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 3811 4605 4625 3811
Size: 794 20 5481
Fragments arranged by size:
5481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 3742 4631
Size: 54 1425 50 275 18 236 1676 889
Cuts at: 4631 6260 8
Size: 1629 43
Fragments arranged by size:
1676 1629 1425 889 275 236 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2637 2703 2858 3543 4225 1353
Size: 1284 66 155 685 682 3423
Fragments arranged by size:
3423 1284 685 682 155 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2604 2661 2850 3137 3593 4358
Size: 393 414 558 57 189 287 456 765
Cuts at: 4358 4955 5742 6010 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 765 597 558 456 414 393
287 268 189 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2541 2541
Size: 6295
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 4936 4968 5010
Size: 32 135 32 447 32 2885 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2885 2626 447 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2288 2416 3639 3885 669
Size: 988 281 350 128 1223 246 3079
Fragments arranged by size:
3079 1223 988 350 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2068 10
Size: 54 1425 343 236 4237
Fragments arranged by size:
4237 1425 343 236 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3317 4253 4612 4771 518
Size: 648 2022 129 936 359 159 2042
Fragments arranged by size:
2042 2022 936 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 3711 4388 5536 5781 1606
Size: 2105 677 1148 245 2120
Fragments arranged by size:
2120 2105 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5743
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NruI TCG'CGA
Cuts at: 2013 2013
Size: 6295
NsiI A_TGCA'T
Cuts at: 2317 3125 2317
Size: 808 5487
NspI r_CATG'y
Cuts at: 1132 1229 3074 3283 3653 5198 1132
Size: 97 1845 209 370 1545 2229
Fragments arranged by size:
2229 1845 1545 370 209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 3279 5194 3279
Size: 1915 4380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspXI vC'TCGA_Gb
Cuts at: 2073 2073
Size: 6295
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2070 2102 2070
Size: 32 6263
PstI C_TGCA'G
Cuts at: 830 2557 3647 830
Size: 1727 1090 3478
Fragments arranged by size:
3478 1727 1090
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2553 2559 3643 4778 5459 5650 826
Size: 219 1508 6 1084 1135 681 191 1471
Fragments arranged by size:
1508 1471 1135 1084 681 219 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 3456 4399 5300 5562 820
Size: 197 926 130 1383 943 901 262 1553
Fragments arranged by size:
1553 1383 943 926 901 262 197 130
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 2348 152
Size: 2196 4099
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 3131 1517
Size: 1614 4681
StyI C'CwwG_G
Cuts at: 513 1065 3132 3734 513
Size: 552 2067 602 3074
Fragments arranged by size:
3074 2067 602 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 3329 4322 4339 4492 4677 5122
Size: 1255 545 1204 993 17 153 185 445
Cuts at: 5122 325
Size: 1498
Fragments arranged by size:
1498 1255 1204 993 545 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4541 6253 96
Size: 274 80 33 51 1443 2564 1712 138
Fragments arranged by size:
2564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3291 3323 6255 6287 3291
Size: 32 2932 32 3299
Fragments arranged by size:
3299 2932 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 2073 1943
Size: 130 6165
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 3281 4424 3281
Size: 1143 5152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI