Restriction map for
pFN21AB9939
(Circular) (Six-base) MAPSORT of: pFN21AB9939.seq Check: 9403 from: 1 to: 6286
pFN21AB9939 6286 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3181 3607 3181
Size: 426 5860
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 4349 5733 6001 1632
Size: 455 2262 1384 268 1917
Fragments arranged by size:
2262 1917 1384 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 3282 4029 5601 3282
Size: 747 1572 3967
Fragments arranged by size:
3967 1572 747
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2318 2356 2489 2585 3167 3216 3595 3821
Size: 825 38 133 96 582 49 379 226
Cuts at: 3821 3973 6082 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 825 582 379 226 152 133
96 49 38
AseI AT'TA_AT
Cuts at: 160 1032 2151 2778 4840 160
Size: 872 1119 627 2062 1606
Fragments arranged by size:
2062 1606 1119 872 627
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 3109 3616 3983 3109
Size: 507 367 5412
Fragments arranged by size:
5412 507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3181 3607 5062 618
Size: 325 217 627 221 1173 426 1455 1842
Fragments arranged by size:
1842 1455 1173 627 426 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2745 3605 1391
Size: 1354 860 4072
Fragments arranged by size:
4072 1354 860
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2819 3238 3261 928
Size: 971 920 419 23 3953
Fragments arranged by size:
3953 971 920 419 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3429 4178 5388 3429
Size: 749 1210 4327
Fragments arranged by size:
4327 1210 749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 4897 136
Size: 107 122 71 631 605 345 2880 1525
Fragments arranged by size:
2880 1525 631 605 345 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4347 5503 1790
Size: 1639 918 1156 2573
Fragments arranged by size:
2573 1639 1156 918
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3377 4017 4975
Size: 416 53 83 399 24 1960 640 958
Cuts at: 4975 442
Size: 1753
Fragments arranged by size:
1960 1753 958 640 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4946 1239
Size: 3707 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4093 4475 5110
Size: 53 83 186 936 476 2084 382 635
Cuts at: 5110 275
Size: 1451
Fragments arranged by size:
2084 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2452 4719 5117 5391 5538 5949 2452
Size: 2267 398 274 147 411 2789
Fragments arranged by size:
2789 2267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2439 2858 1186
Size: 1253 419 4614
Fragments arranged by size:
4614 1253 419
BseYI C'CCAG_C
Cuts at: 5489 5985 5489
Size: 496 5790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 2970 3295 1163
Size: 573 598 636 325 4154
Fragments arranged by size:
4154 636 598 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 4347 4432 5503 2256
Size: 2091 85 1071 3039
Fragments arranged by size:
3039 2091 1071 85
BsmI GAATG_Cn'
Cuts at: 2228 3488 3797 3890 2228
Size: 1260 309 93 4624
Fragments arranged by size:
4624 1260 309 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2745 3429 3605 4347 4432 5503
Size: 399 466 489 684 176 742 85 1071
Cuts at: 5503 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 684 489 466 399 176
85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 3641 844
Size: 2119 678 3489
Fragments arranged by size:
3489 2119 678
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 4173 1394
Size: 429 194 2156 3507
Fragments arranged by size:
3507 2156 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 3441 4781 4955 65
Size: 1183 2193 1340 174 1396
Fragments arranged by size:
2193 1396 1340 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 3227 96
Size: 2202 929 3155
Fragments arranged by size:
3155 2202 929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3282 794
Size: 2488 3798
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstXI CCAn_nnnn'nTGG
Cuts at: 3390 3390
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2599 3109 3616 3983 4368 4385 5837 5923
Size: 627 510 507 367 385 17 1452 86
Cuts at: 5923 5935 6281 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 627 510 507 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 513
Size: 552 939 64 4731
Fragments arranged by size:
4731 939 552 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4596 4616 3802
Size: 794 20 5472
Fragments arranged by size:
5472 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251
Size: 54 1425 50 275 18 1903 889 1629
Cuts at: 6251 8
Size: 43
Fragments arranged by size:
1903 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2835 4216 1353
Size: 753 729 1381 3423
Fragments arranged by size:
3423 1381 753 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 4873 5247 5393 387
Size: 1064 138 440 2844 374 146 1280
Fragments arranged by size:
2844 1280 1064 440 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 4349 4946 5733 6001 1239
Size: 393 455 2262 597 787 268 1524
Fragments arranged by size:
2262 1524 787 597 455 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2424 2792 2424
Size: 368 5918
EcoRI G'AATT_C
Cuts at: 3167 3216 3595 3973 3167
Size: 49 379 378 5480
Fragments arranged by size:
5480 379 378 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 3355 2188
Size: 1167 5119
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 2012 2036 5433 1678
Size: 334 24 3397 2531
Fragments arranged by size:
3397 2531 334 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2449 2481 3403 3435 4927
Size: 32 135 32 877 32 922 32 1492
Cuts at: 4927 4959 5001 5033 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1492 922 877 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3012 748
Size: 2264 4022
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6286
KpnI G_GTAC'C
Cuts at: 3185 3611 3185
Size: 426 5860
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 2868 2975 5400 5584 1305
Size: 1336 227 107 2425 184 2007
Fragments arranged by size:
2425 2007 1336 227 184 107
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4464
Fragments arranged by size:
4464 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 2472 2715 4244 4603 4762 518
Size: 648 1267 39 243 1529 359 159 2042
Fragments arranged by size:
2042 1529 1267 648 359 243 159 39
MspA1I CmG'CkG
Cuts at: 1606 3702 4379 5527 5772 1606
Size: 2096 677 1148 245 2120
Fragments arranged by size:
2120 2096 1148 677 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6286
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4795
Fragments arranged by size:
4795 939 552
NdeI CA'TA_TG
Cuts at: 387 2992 3397 387
Size: 2605 405 3276
Fragments arranged by size:
3276 2605 405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2670 3644 5189 1132
Size: 97 1441 974 1545 2229
Fragments arranged by size:
2229 1545 1441 974 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3518 1108
Size: 2410 3876
PfoI T'CCnGG_A
Cuts at: 2217 3112 2217
Size: 895 5391
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2424 2792 2424
Size: 368 5918
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SapI GCTCTTCn'nnn_
Cuts at: 2106 2106
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 2752 3224 4534 6246 2752
Size: 472 1310 1712 2792
Fragments arranged by size:
2792 1712 1310 472
SexAI A'CCwGG_T
Cuts at: 2892 3097 2892
Size: 205 6081
SfcI C'TryA_G
Cuts at: 826 1045 2247 3634 4769 5450 5641 826
Size: 219 1202 1387 1135 681 191 1471
Fragments arranged by size:
1471 1387 1202 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6286
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4390 5291 5553 820
Size: 197 926 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 2670 3644 1132
Size: 1538 974 3774
Fragments arranged by size:
3774 1538 974
SspI AAT'ATT
Cuts at: 5 52 2776 4210 5
Size: 47 2724 1434 2081
Fragments arranged by size:
2724 2081 1434 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 2004 3134 3346 3725 513
Size: 552 939 1130 212 379 3074
Fragments arranged by size:
3074 1130 939 552 379 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2749 4313 4330 4483 4668 5113 325
Size: 1255 1169 1564 17 153 185 445 1498
Fragments arranged by size:
1564 1498 1255 1169 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 2525 2750
Size: 274 80 33 51 1443 321 227 225
Cuts at: 2750 3222 3227 4532 6244 96
Size: 472 5 1305 1712 138
Fragments arranged by size:
1712 1443 1305 472 321 274 227 225
138 80 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2100 2913 4415 2100
Size: 813 1502 3971
Fragments arranged by size:
3971 1502 813
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SrfI SwaI XcmI