Restriction map for
pFN21AB9936
(Circular) (Six-base) MAPSORT of: pFN21AB9936.seq Check: 2384 from: 1 to: 6607
pFN21AB9936 6607 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3765 4417 278
Size: 53 83 186 936 2229 652 2468
Fragments arranged by size:
2468 2229 936 652 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3950 1937
Size: 2013 4594
Acc65I G'GTAC_C
Cuts at: 3253 3928 3253
Size: 675 5932
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4670 6054 6322 1632
Size: 3038 1384 268 1917
Fragments arranged by size:
3038 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 3462 5506 6483 1203
Size: 2259 2044 977 1327
Fragments arranged by size:
2259 2044 1327 977
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 3034 5336 3034
Size: 2302 4305
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3516 1184
Size: 2332 4275
AlwNI CAG_nnn'CTG
Cuts at: 2155 3052 3652 4350 5922 2155
Size: 897 600 698 1572 2840
Fragments arranged by size:
2840 1572 897 698 600
ApaI G_GGCC'C
Cuts at: 2168 2168
Size: 6607
ApaLI G'TGCA_C
Cuts at: 2528 4664 5820 2528
Size: 2136 1156 3315
Fragments arranged by size:
3315 2136 1156
ApoI r'AATT_y
Cuts at: 1493 2030 3916 4142 4294 6403 1493
Size: 537 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2263 2795 3571 3932 1870
Size: 73 320 532 776 361 4545
Fragments arranged by size:
4545 776 532 361 320 73
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2292 2405 2879 3166
Size: 325 217 627 438 67 113 474 287
Cuts at: 3166 3253 3325 3928 5383 618
Size: 87 72 603 1455 1842
Fragments arranged by size:
1842 1455 627 603 474 438 325 287
217 113 87 72 67
BanII G_rGCy'C
Cuts at: 1391 2168 2805 3083 3422 3578 3926 1391
Size: 777 637 278 339 156 348 4072
Fragments arranged by size:
4072 777 637 348 339 278 156
BbeI G_GCGC'C
Cuts at: 2883 3170 3329 2883
Size: 287 159 6161
Fragments arranged by size:
6161 287 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 928
Size: 971 734 4902
Fragments arranged by size:
4902 971 734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4499 5709 3650
Size: 849 1210 4548
Fragments arranged by size:
4548 1210 849
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2337 2403 2931
Size: 107 122 71 631 605 665 66 528
Cuts at: 2931 3494 3618 3672 5218 136
Size: 563 124 54 1546 1525
Fragments arranged by size:
1546 1525 665 631 605 563 528 124
122 107 71 66 54
BglII A'GATC_T
Cuts at: 6602 6602
Size: 6607
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2532 4668 5824 1790
Size: 378 364 2136 1156 2573
Fragments arranged by size:
2573 2136 1156 378 364
BmgBI CAC'GTC
Cuts at: 1318 2527 1318
Size: 1209 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 2171 3265
Size: 416 53 83 399 24 726 28 1094
Cuts at: 3265 3662 4338 5296 442
Size: 397 676 958 1753
Fragments arranged by size:
1753 1094 958 726 676 416 399 397
83 53 28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2123 2461 2812 3062 3538 3539 5267
Size: 878 6 338 351 250 476 1 1728
Cuts at: 5267 1239
Size: 2579
Fragments arranged by size:
2579 1728 878 476 351 338 250 6
1
Bpu10I CC'TnA_GC
Cuts at: 2108 2951 2108
Size: 843 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3788 4732 5597 5895 3788
Size: 944 865 298 4500
Fragments arranged by size:
4500 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 5270 882
Size: 617 1068 2703 2219
Fragments arranged by size:
2703 2219 1068 617
BsaAI yAC'GTr
Cuts at: 493 1204 4398 493
Size: 711 3194 2702
Fragments arranged by size:
3194 2702 711
BsaBI GATnn'nnATC
Cuts at: 3308 3314 6601 3308
Size: 6 3287 3314
Fragments arranged by size:
3314 3287 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 2880 2934 2968
Size: 53 83 186 936 953 394 54 34
Cuts at: 2968 3030 3167 3326 3762 4414 4796 5431 275
Size: 62 137 159 436 652 382 635 1451
Fragments arranged by size:
1451 953 936 652 635 436 394 382
186 159 137 83 62 54 53 34
BsaWI w'CCGG_w
Cuts at: 3891 5040 5438 5712 5859 6270 3891
Size: 1149 398 274 147 411 4228
Fragments arranged by size:
4228 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 5002
Fragments arranged by size:
5002 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2105 2813 2899 3111 1186
Size: 901 18 708 86 212 4682
Fragments arranged by size:
4682 901 708 212 86 18
BseYI C'CCAG_C
Cuts at: 2168 2926 3084 3105 3135 3203 3716 3871 5810
Size: 758 158 21 30 68 513 155 1939
Cuts at: 5810 6306 2168
Size: 496 2469
Fragments arranged by size:
2469 1939 758 513 496 158 155 68
30 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2453 2799 3818 1163
Size: 573 300 417 346 1019 3952
Fragments arranged by size:
3952 1019 573 417 346 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4057 4818 5846 1540
Size: 275 186 2056 761 1028 2301
Fragments arranged by size:
2301 2056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2480 2532 2900 2957 3422 4668 4753 5824 2480
Size: 52 368 57 465 1246 85 1071 3263
Fragments arranged by size:
3263 1246 1071 465 368 85 57 52
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2069
Size: 6607
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2480 2532 2805 2900 2957 3083
Size: 399 378 312 52 273 95 57 126
Cuts at: 3083 3422 3578 3926 4668 4753 5824 1391
Size: 339 156 348 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 348 339 312
273 156 126 95 85 57 52
BspEI T'CCGG_A
Cuts at: 3891 6270 3891
Size: 2379 4228
BspHI T'CATG_A
Cuts at: 2097 4496 6226 2097
Size: 2399 1730 2478
Fragments arranged by size:
2478 2399 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2244 3962 844
Size: 1319 81 1718 3489
Fragments arranged by size:
3489 1718 1319 81
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 4494 1394
Size: 429 953 1718 3507
Fragments arranged by size:
3507 1718 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2331 2352 2837 2882 3328 3356 5251
Size: 110 521 21 485 45 446 28 1895
Cuts at: 5251 5438 1700
Size: 187 2869
Fragments arranged by size:
2869 1895 521 485 446 187 110 45
28 21
BsrGI T'GTAC_A
Cuts at: 96 3564 96
Size: 3468 3139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 3219 4667 5679 3219
Size: 1448 1012 4147
Fragments arranged by size:
4147 1448 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3052 794
Size: 2258 4349
BstBI TT'CG_AA
Cuts at: 2015 5411 2015
Size: 3396 3211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 3745 2186
Size: 1559 5048
BstYI r'GATC_y
Cuts at: 1972 3937 4304 4689 4706 6158 6244 6256 6602
Size: 1965 367 385 17 1452 86 12 346
Cuts at: 6602 1972
Size: 1977
Fragments arranged by size:
1977 1965 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 2229 2296 2427 3024 3625
Size: 552 939 20 205 67 131 597 601
Cuts at: 3625 513
Size: 3495
Fragments arranged by size:
3495 939 601 597 552 205 131 67
20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2964 3301 487
Size: 683 56 1738 337 3793
Fragments arranged by size:
3793 1738 683 337 56
BtsI GCAGTG_nn'
Cuts at: 2518 4123 4917 4937 2518
Size: 1605 794 20 4188
Fragments arranged by size:
4188 1605 794 20
ClaI AT'CG_AT
Cuts at: 1554 3302 1554
Size: 1748 4859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3706 5614 809
Size: 507 2390 1908 1802
Fragments arranged by size:
2390 1908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 2850 2884
Size: 54 1425 50 275 18 499 521 34
Cuts at: 2884 3292 3366 3486 3600 3747 3864 4054 4943
Size: 408 74 120 114 147 117 190 889
Cuts at: 4943 6572 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 521 499 408 275 190
147 120 117 114 74 54 50 43
34 18
EagI C'GGCC_G
Cuts at: 1537 1812 4054 1537
Size: 275 2242 4090
Fragments arranged by size:
4090 2242 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 4537 1353
Size: 1212 1972 3423
Fragments arranged by size:
3423 1972 1212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 2452 3164 5194 5568 5714
Size: 1064 138 495 368 712 2030 374 146
Cuts at: 5714 387
Size: 1280
Fragments arranged by size:
2030 1280 1064 712 495 374 368 146
138
EcoICRI GAG'CTC
Cuts at: 3420 3420
Size: 6607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2123 2461 2812 3062 3538 3539
Size: 393 485 6 338 351 250 476 1
Cuts at: 3539 4670 5267 6054 6322 1239
Size: 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 787 597 485 476 393 351
338 268 250 6 1
EcoO109I rG'GnC_Cy
Cuts at: 2104 2165 2193 2517 2656 2736 2104
Size: 61 28 324 139 80 5975
Fragments arranged by size:
5975 324 139 80 61 28
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2883 3170 3210 3329 3357 5754 1678
Size: 1205 287 40 119 28 2397 2531
Fragments arranged by size:
2531 2397 1205 287 119 40 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 3020 3052 3305
Size: 32 135 32 992 32 424 32 253
Cuts at: 3305 3337 3727 3759 5248 5280 5322 5354 1373
Size: 32 390 32 1489 32 42 32 2626
Fragments arranged by size:
2626 1489 992 424 390 253 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 3951 4197 669
Size: 988 281 615 1398 246 3079
Fragments arranged by size:
3079 1398 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 2553 4197 2553
Size: 1644 4963
KasI G'GCGC_C
Cuts at: 2879 3166 3325 2879
Size: 287 159 6161
Fragments arranged by size:
6161 287 159
KpnI G_GTAC'C
Cuts at: 3257 3932 3257
Size: 675 5932
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 3462 6483 3462
Size: 3021 3586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3801 5721 5905 1305
Size: 2496 1920 184 2007
Fragments arranged by size:
2496 2007 1920 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3294 10
Size: 54 1425 343 1462 3323
Fragments arranged by size:
3323 1462 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4565 4924 5083 518
Size: 648 3399 359 159 2042
Fragments arranged by size:
3399 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2394 2429 2439 2788 2930 2998 3052
Size: 420 368 35 10 349 142 68 54
Cuts at: 3052 3517 4023 4700 5848 6093 1606
Size: 465 506 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 506 465 420 368 349
245 142 68 54 35 10
NaeI GCC'GGC
Cuts at: 2333 2354 2884 3330 3358 2333
Size: 21 530 446 28 5582
Fragments arranged by size:
5582 530 446 28 21
NarI GG'CG_CC
Cuts at: 2880 3167 3326 2880
Size: 287 159 6161
Fragments arranged by size:
6161 287 159
NcoI C'CATG_G
Cuts at: 513 1065 2004 2229 2296 3024 513
Size: 552 939 225 67 728 4096
Fragments arranged by size:
4096 939 728 552 225 67
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NgoMIV G'CCGG_C
Cuts at: 2331 2352 2882 3328 3356 2331
Size: 21 530 446 28 5582
Fragments arranged by size:
5582 530 446 28 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 3841 3965 5510 1132
Size: 97 2612 124 1545 2229
Fragments arranged by size:
2612 2229 1545 124 97
PasI CC'CwG_GG
Cuts at: 1348 3413 3442 1348
Size: 2065 29 4513
Fragments arranged by size:
4513 2065 29
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 3517 1108
Size: 1194 1215 4198
Fragments arranged by size:
4198 1215 1194
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2193 2517 2656 2736 2104
Size: 89 324 139 80 5975
Fragments arranged by size:
5975 324 139 89 80
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspOMI G'GGCC_C
Cuts at: 2164 2164
Size: 6607
PstI C_TGCA'G
Cuts at: 830 3339 3959 830
Size: 2509 620 3478
Fragments arranged by size:
3478 2509 620
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2394 2439 2930 3052 3517 2394
Size: 45 491 122 465 5484
Fragments arranged by size:
5484 491 465 122 45
SacI G_AGCT'C
Cuts at: 3422 3422
Size: 6607
SacII CC_GC'GG
Cuts at: 2027 2430 2027
Size: 403 6204
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 2193 2517 2736 2193
Size: 324 219 6064
Fragments arranged by size:
6064 324 219
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 2183 4855 6567 2183
Size: 2672 1712 2223
Fragments arranged by size:
2672 2223 1712
SfcI C'TryA_G
Cuts at: 826 1045 2700 3018 3335 3955 5090 5771 5962
Size: 219 1655 318 317 620 1135 681 191
Cuts at: 5962 826
Size: 1471
Fragments arranged by size:
1655 1471 1135 681 620 318 317 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3494 3494
Size: 6607
SfoI GGC'GCC
Cuts at: 2881 3168 3327 2881
Size: 287 159 6161
Fragments arranged by size:
6161 287 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3573 3934 1872
Size: 1701 361 4545
Fragments arranged by size:
4545 1701 361
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3803 4711 5612 5874 820
Size: 197 926 1860 908 901 262 1553
Fragments arranged by size:
1860 1553 926 908 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SrfI GCCC'GGGC
Cuts at: 3573 3573
Size: 6607
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6607
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2296 3024 4046 513
Size: 552 939 225 67 728 1022 3074
Fragments arranged by size:
3074 1022 939 728 552 225 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 3019 3086 4634 4651 4804 4989
Size: 1255 1155 284 67 1548 17 153 185
Cuts at: 4989 5434 325
Size: 445 1498
Fragments arranged by size:
1548 1498 1255 1155 445 284 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3564 4853
Size: 274 80 33 51 1443 204 1383 1289
Cuts at: 4853 6565 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1383 1289 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 6567 6599 2753
Size: 32 3782 32 2761
Fragments arranged by size:
3782 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2632 1316
Size: 1316 5291
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2219 2219
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3571 3932 1870
Size: 1701 361 4545
Fragments arranged by size:
4545 1701 361
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3763 4415 276
Size: 53 83 186 936 2229 652 2468
Fragments arranged by size:
2468 2229 936 652 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SapI SexAI SwaI