Restriction map for
pFN21AB9932
(Circular) (Six-base) MAPSORT of: pFN21AB9932.seq Check: 1820 from: 1 to: 6619
pFN21AB9932 6619 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3208 3220 3208
Size: 12 6607
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3834 3962 1937
Size: 1897 128 4594
Fragments arranged by size:
4594 1897 128
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 2597 4682 6066 6334 1632
Size: 448 517 2085 1384 268 1917
Fragments arranged by size:
2085 1917 1384 517 448 268
AflII C'TTAA_G
Cuts at: 820 1017 3049 820
Size: 197 2032 4390
Fragments arranged by size:
4390 2032 197
AflIII A'CryG_T
Cuts at: 1203 5518 6495 1203
Size: 4315 977 1327
Fragments arranged by size:
4315 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 3258 1166
Size: 2092 4527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AlwNI CAG_nnn'CTG
Cuts at: 2294 4362 5934 2294
Size: 2068 1572 2979
Fragments arranged by size:
2979 2068 1572
ApaLI G'TGCA_C
Cuts at: 2155 2425 4676 5832 2155
Size: 270 2251 1156 2942
Fragments arranged by size:
2942 2251 1156 270
ApoI r'AATT_y
Cuts at: 1493 2889 3494 3928 4154 4306 6415 1493
Size: 1396 605 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 1396 605 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2896 3944 1870
Size: 73 953 1048 4545
Fragments arranged by size:
4545 1048 953 73
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3252 3940 5395 618
Size: 325 217 627 1465 688 1455 1842
Fragments arranged by size:
1842 1465 1455 688 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3107 3938 1391
Size: 1716 831 4072
Fragments arranged by size:
4072 1716 831
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2765 3024 928
Size: 971 866 259 4523
Fragments arranged by size:
4523 971 866 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4810 4844 1903
Size: 34 2873 34 3678
Fragments arranged by size:
3678 2873 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2429 2590 3814 4680 5836 1790
Size: 369 270 161 1224 866 1156 2573
Fragments arranged by size:
2573 1224 1156 866 369 270 161
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4350 5308
Size: 416 53 83 399 24 1727 1206 958
Cuts at: 5308 442
Size: 1753
Fragments arranged by size:
1753 1727 1206 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3287
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 3455 5279 1239
Size: 1413 803 1824 2579
Fragments arranged by size:
2579 1824 1413 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2802 4744 5609 5907 2802
Size: 1942 865 298 3514
Fragments arranged by size:
3514 1942 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5282 882
Size: 617 3783 2219
Fragments arranged by size:
3783 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2490 3563 5052 5450 5724 5871 6282 2490
Size: 1073 1489 398 274 147 411 2827
Fragments arranged by size:
2827 1489 1073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2937 2967 3094
Size: 30 165 30 807 30 697 30 127
Cuts at: 3094 3124 1178
Size: 30 4673
Fragments arranged by size:
4673 807 697 165 127 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2385 2805 2928 1186
Size: 1199 420 123 4877
Fragments arranged by size:
4877 1199 420 123
BseYI C'CCAG_C
Cuts at: 3795 5822 6318 3795
Size: 2027 496 4096
Fragments arranged by size:
4096 2027 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2307 2680 3136 1163
Size: 573 571 373 456 4646
Fragments arranged by size:
4646 573 571 456 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2159 2429 3107 4680 4765 5836 2159
Size: 270 678 1573 85 1071 2942
Fragments arranged by size:
2942 1573 1071 678 270 85
BsmI GAATG_Cn'
Cuts at: 2417 3650 4130 4223 2417
Size: 1233 480 93 4813
Fragments arranged by size:
4813 1233 480 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2429 2590 3107 3814 3938 4680
Size: 399 369 270 161 517 707 124 742
Cuts at: 4680 4765 5836 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 517 399 369 270
161 124 85
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 3448 4508 6238 3448
Size: 1060 1730 3829
Fragments arranged by size:
3829 1730 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 3208 3220 3974 844
Size: 2017 347 12 754 3489
Fragments arranged by size:
3489 2017 754 347 12
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2318 5114 5288 65
Size: 1183 1070 2796 174 1396
Fragments arranged by size:
2796 1396 1183 1070 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5263 5450 1700
Size: 110 3453 187 2869
Fragments arranged by size:
3453 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2121 96
Size: 2025 4594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 3559 1184
Size: 2375 4244
BstYI r'GATC_y
Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614
Size: 1977 367 385 17 1452 86 12 346
Cuts at: 6614 1972
Size: 1977
Fragments arranged by size:
1977 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 3294 3304 3892 4135 4929 4949 2407
Size: 887 10 588 243 794 20 4077
Fragments arranged by size:
4077 887 794 588 243 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3360 3923 3360
Size: 563 6056
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3146 5626 809
Size: 507 1830 2480 1802
Fragments arranged by size:
2480 1830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584
Size: 54 1425 50 275 18 2236 889 1629
Cuts at: 6584 8
Size: 43
Fragments arranged by size:
2236 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2723 3024 3199 4549 1353
Size: 1370 301 175 1350 3423
Fragments arranged by size:
3423 1370 1350 301 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3105 3105
Size: 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2597 2652 3455 4682 5279 6066
Size: 393 448 517 55 803 1227 597 787
Cuts at: 6066 6334 1239
Size: 268 1524
Fragments arranged by size:
1524 1227 803 787 597 517 448 393
268 55
EcoNI CCTnn'n_nnAGG
Cuts at: 3522 3522
Size: 6619
EcoO109I rG'GnC_Cy
Cuts at: 3289 3289
Size: 6619
EcoRI G'AATT_C
Cuts at: 3494 3928 4306 3494
Size: 434 378 5807
Fragments arranged by size:
5807 434 378
EcoRV GAT'ATC
Cuts at: 3454 3659 3454
Size: 205 6414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3064 3064
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 5766 1678
Size: 4088 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2971 3003 5260 5292 5334
Size: 32 135 32 1399 32 2257 32 42
Cuts at: 5334 5366 1373
Size: 32 2626
Fragments arranged by size:
2626 2257 1399 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2905 3963 4209 669
Size: 988 281 967 1058 246 3079
Fragments arranged by size:
3079 1058 988 967 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 2497 4218 2497
Size: 1721 4898
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2666 3098 3160 5733 5917 1305
Size: 1361 432 62 2573 184 2007
Fragments arranged by size:
2573 2007 1361 432 184 62
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4797
Fragments arranged by size:
4797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2975 3258 4577 4936 5095 518
Size: 648 1809 283 1319 359 159 2042
Fragments arranged by size:
2042 1809 1319 648 359 283 159
MspA1I CmG'CkG
Cuts at: 1606 3795 4035 4712 5860 6105 1606
Size: 2189 240 677 1148 245 2120
Fragments arranged by size:
2189 2120 1148 677 245 240
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6067
NdeI CA'TA_TG
Cuts at: 387 2458 387
Size: 2071 4548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 3977 5522 1132
Size: 97 2748 1545 2229
Fragments arranged by size:
2748 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3555 3685 1108
Size: 2447 130 4042
Fragments arranged by size:
4042 2447 130
PfoI T'CCnGG_A
Cuts at: 2518 2518
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3289 3289
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 2352 3669 4189 2352
Size: 1317 520 4782
Fragments arranged by size:
4782 1317 520
PstI C_TGCA'G
Cuts at: 830 3231 3330 3971 830
Size: 2401 99 641 3478
Fragments arranged by size:
3478 2401 641 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 3795 3795
Size: 6619
SacI G_AGCT'C
Cuts at: 3107 3107
Size: 6619
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SbfI CC_TGCA'GG
Cuts at: 3231 3330 3971 3231
Size: 99 641 5879
Fragments arranged by size:
5879 641 99
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SexAI A'CCwGG_T
Cuts at: 3826 3826
Size: 6619
SfcI C'TryA_G
Cuts at: 826 1045 2049 3227 3326 3346 3967 5102 5783
Size: 219 1004 1178 99 20 621 1135 681
Cuts at: 5783 5974 826
Size: 191 1471
Fragments arranged by size:
1471 1178 1135 1004 681 621 219 191
99 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 2898 3946 1872
Size: 1026 1048 4545
Fragments arranged by size:
4545 1048 1026
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2817 3049 4723 5624 5886 820
Size: 197 926 874 232 1674 901 262 1553
Fragments arranged by size:
1674 1553 926 901 874 262 232 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 3604 3622 4543 5
Size: 47 3552 18 921 2081
Fragments arranged by size:
3552 2081 921 47 18
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 2168 4058 513
Size: 552 1103 1890 3074
Fragments arranged by size:
3074 1890 1103 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2181 4646 4663 4816 5001 5446 325
Size: 1255 601 2465 17 153 185 445 1498
Fragments arranged by size:
2465 1498 1255 601 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 2272 2511
Size: 274 80 33 51 1443 144 151 239
Cuts at: 2511 3852 4865 6577 96
Size: 1341 1013 1712 138
Fragments arranged by size:
1712 1443 1341 1013 274 239 151 144
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3676 3708 6579 6611 3020
Size: 32 624 32 2871 32 3028
Fragments arranged by size:
3028 2871 624 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3311 3311
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 2896 3944 1870
Size: 1026 1048 4545
Fragments arranged by size:
4545 1048 1026
XmnI GAAnn'nnTTC
Cuts at: 2618 4748 2618
Size: 2130 4489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI