Restriction map for
pFN21AB9928
(Circular) (Six-base) MAPSORT of: pFN21AB9928.seq Check: 1530 from: 1 to: 6583
pFN21AB9928 6583 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2810 2984 3389 3770 4646 6030 6298
Size: 432 746 174 405 381 876 1384 268
Cuts at: 6298 1632
Size: 1917
Fragments arranged by size:
1917 1384 876 746 432 405 381 268
174
AfeI AGC'GCT
Cuts at: 2521 2521
Size: 6583
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 3465 5482 6459 1203
Size: 2262 2017 977 1327
Fragments arranged by size:
2262 2017 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 4326 5898 4326
Size: 1572 5011
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 2428 2806 3892 4118 4270 6379 1493
Size: 935 378 1086 226 152 2109 1697
Fragments arranged by size:
2109 1697 1086 935 378 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 3304 3908 1870
Size: 73 1361 604 4545
Fragments arranged by size:
4545 1361 604 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3471 3904 5359 618
Size: 325 217 627 1684 433 1455 1842
Fragments arranged by size:
1842 1684 1455 627 433 325 217
BanII G_rGCy'C
Cuts at: 1391 3702 3902 1391
Size: 2311 200 4072
Fragments arranged by size:
4072 2311 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 5219
Fragments arranged by size:
5219 971 393
BbvCI CC'TCA_GC
Cuts at: 3383 3446 3383
Size: 63 6520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2754 2788 4774 4808 1903
Size: 34 817 34 1986 34 3678
Fragments arranged by size:
3678 1986 817 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2354 4475 5685 2354
Size: 2121 1210 3252
Fragments arranged by size:
3252 2121 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3075 5194 136
Size: 107 122 71 631 605 1403 2119 1525
Fragments arranged by size:
2119 1525 1403 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 4644 5800 1790
Size: 2854 1156 2573
Fragments arranged by size:
2854 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3466 1318
Size: 2148 4435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3665 3742 4314
Size: 416 53 83 399 24 2248 77 572
Cuts at: 4314 5272 442
Size: 958 1753
Fragments arranged by size:
2248 1753 958 572 416 399 83 77
53 24
BmtI G_CTAG'C
Cuts at: 1056 2520 1056
Size: 1464 5119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2690 2948 3240 3673 3785 3821 5243 1239
Size: 1451 258 292 433 112 36 1422 2579
Fragments arranged by size:
2579 1451 1422 433 292 258 112 36
Bpu10I CC'TnA_GC
Cuts at: 2814 3383 3446 2814
Size: 569 63 5951
Fragments arranged by size:
5951 569 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3338 4708 5573 5871 3338
Size: 1370 865 298 4050
Fragments arranged by size:
4050 1370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3155 3239 3777 5246 882
Size: 617 1656 84 538 1469 2219
Fragments arranged by size:
2219 1656 1469 617 538 84
BsaAI yAC'GTr
Cuts at: 493 1204 2307 4374 493
Size: 711 1103 2067 2702
Fragments arranged by size:
2702 2067 1103 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4390 4772 5407 275
Size: 53 83 186 936 2857 382 635 1451
Fragments arranged by size:
2857 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2326 2464 5016 5414 5688 5835 6246 2326
Size: 138 2552 398 274 147 411 2663
Fragments arranged by size:
2663 2552 411 398 274 147 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 2921 2951 3219
Size: 30 165 30 1266 30 222 30 268
Cuts at: 3219 3249 3434 3464 1178
Size: 30 185 30 4297
Fragments arranged by size:
4297 1266 268 222 185 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 3227 3676 1186
Size: 957 1084 449 4093
Fragments arranged by size:
4093 1084 957 449
BseYI C'CCAG_C
Cuts at: 2688 5786 6282 2688
Size: 3098 496 2989
Fragments arranged by size:
3098 2989 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3022 3752 1163
Size: 573 1286 730 3994
Fragments arranged by size:
3994 1286 730 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 4033 4794 5822 1540
Size: 275 186 1078 954 761 1028 2301
Fragments arranged by size:
2301 1078 1028 954 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 4644 4729 5800 2012
Size: 2632 85 1071 2795
Fragments arranged by size:
2795 2632 1071 85
BsmI GAATG_Cn'
Cuts at: 2060 4094 4187 2060
Size: 2034 93 4456
Fragments arranged by size:
4456 2034 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3702 3902 4644 4729 5800 1391
Size: 399 222 1690 200 742 85 1071 2174
Fragments arranged by size:
2174 1690 1071 742 399 222 200 85
BspEI T'CCGG_A
Cuts at: 2326 6246 2326
Size: 3920 2663
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3938 844
Size: 3094 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4470 1394
Size: 429 2647 3507
Fragments arranged by size:
3507 2647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2443 3570 5078 5252 65
Size: 1183 1195 1127 1508 174 1396
Fragments arranged by size:
1508 1396 1195 1183 1127 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5227 5414 1700
Size: 110 3417 187 2869
Fragments arranged by size:
3417 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 4643 5655 4643
Size: 1012 5571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2256 3190 3315 794
Size: 1462 934 125 4062
Fragments arranged by size:
4062 1462 934 125
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2066 2861 3460 2066
Size: 795 599 5189
Fragments arranged by size:
5189 795 599
BstYI r'GATC_y
Cuts at: 1972 3155 3852 3913 4280 4665 4682 6134 6220
Size: 1183 697 61 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1183 697 385 367 346 86
61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2065 513
Size: 552 1000 5031
Fragments arranged by size:
5031 1000 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5844
Fragments arranged by size:
5844 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 2455 4099 4893 4913 2173
Size: 282 1644 794 20 3843
Fragments arranged by size:
3843 1644 794 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3076 3817 4030
Size: 54 1425 50 275 18 1246 741 213
Cuts at: 4030 4919 6548 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1246 889 741 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 4030 1537
Size: 275 1264 954 4090
Fragments arranged by size:
4090 1264 954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2967 4513 1353
Size: 1614 1546 3423
Fragments arranged by size:
3423 1614 1546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3013 5170 5544 5690 387
Size: 1064 138 1424 2157 374 146 1280
Fragments arranged by size:
2157 1424 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2690 2810 2948 2984 3240 3389
Size: 393 432 626 120 138 36 256 149
Cuts at: 3389 3673 3770 3785 3821 4646 5243 6030 6298
Size: 284 97 15 36 825 597 787 268
Cuts at: 6298 1239
Size: 1524
Fragments arranged by size:
1524 825 787 626 597 432 393 284
268 256 149 138 120 97 36 36
15
EcoNI CCTnn'n_nnAGG
Cuts at: 3710 3710
Size: 6583
EcoO109I rG'GnC_Cy
Cuts at: 3358 3358
Size: 6583
EcoRI G'AATT_C
Cuts at: 2428 3892 4270 2428
Size: 1464 378 4741
Fragments arranged by size:
4741 1464 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 6583
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 2164 2523 5730 1678
Size: 486 359 3207 2531
Fragments arranged by size:
3207 2531 486 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 2807 2839 2893
Size: 32 135 32 547 32 656 32 54
Cuts at: 2893 2925 3219 3251 3434 3466 5224 5256 5298
Size: 32 294 32 183 32 1758 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 656 547 294 183 135 54
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3927 4173 669
Size: 988 281 1168 821 246 3079
Fragments arranged by size:
3079 1168 988 821 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5697 5881 1305
Size: 4392 184 2007
Fragments arranged by size:
4392 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3819 10
Size: 54 1425 343 1987 2774
Fragments arranged by size:
2774 1987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2448 3458 3671 4541 4900 5059
Size: 648 898 384 1010 213 870 359 159
Cuts at: 5059 518
Size: 2042
Fragments arranged by size:
2042 1010 898 870 648 384 359 213
159
MspA1I CmG'CkG
Cuts at: 1606 2857 3999 4676 5824 6069 1606
Size: 1251 1142 677 1148 245 2120
Fragments arranged by size:
2120 1251 1148 1142 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2065 513
Size: 552 1000 5031
Fragments arranged by size:
5031 1000 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 2516 1052
Size: 1464 5119
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2499 3941 5486 1132
Size: 97 1270 1442 1545 2229
Fragments arranged by size:
2229 1545 1442 1270 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6583
PfoI T'CCnGG_A
Cuts at: 2109 2508 3042 2109
Size: 399 534 5650
Fragments arranged by size:
5650 534 399
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PmlI CAC'GTG
Cuts at: 2307 2307
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3358 3358
Size: 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 2550 4153 2550
Size: 1603 4980
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 6551
PstI C_TGCA'G
Cuts at: 830 3195 3935 830
Size: 2365 740 3478
Fragments arranged by size:
3478 2365 740
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2857 2857
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SapI GCTCTTCn'nnn_
Cuts at: 2967 2967
Size: 6583
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 3323 4831 6543 3323
Size: 1508 1712 3363
Fragments arranged by size:
3363 1712 1508
SfcI C'TryA_G
Cuts at: 826 1045 3191 3931 5066 5747 5938 826
Size: 219 2146 740 1135 681 191 1471
Fragments arranged by size:
2146 1471 1135 740 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3353 4687 5588 5850 820
Size: 197 926 1410 1334 901 262 1553
Fragments arranged by size:
1553 1410 1334 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 2499 3941 1132
Size: 1367 1442 3774
Fragments arranged by size:
3774 1442 1367
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 3260 3824 3866 1517
Size: 1743 564 42 4234
Fragments arranged by size:
4234 1743 564 42
StyI C'CwwG_G
Cuts at: 513 1065 2065 3270 3291 4022 513
Size: 552 1000 1205 21 731 3074
Fragments arranged by size:
3074 1205 1000 731 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 4610 4627 4780 4965 5410 325
Size: 1255 2277 753 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 753 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 4829 6541
Size: 274 80 33 51 1443 1344 1508 1712
Cuts at: 6541 96
Size: 138
Fragments arranged by size:
1712 1508 1443 1344 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2921 2953 3455 3487 3782 3814 6543 6575 2921
Size: 32 502 32 295 32 2729 32 2929
Fragments arranged by size:
2929 2729 502 295 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3231 1316
Size: 1915 4668
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2923 2186
Size: 737 5846
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI