Restriction map for
pFN21AB9914
(Circular) (Six-base) MAPSORT of: pFN21AB9914.seq Check: 2944 from: 1 to: 6304
pFN21AB9914 6304 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2613 3539 4114 278
Size: 53 83 186 936 1077 926 575 2468
Fragments arranged by size:
2468 1077 936 926 575 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3625 3625
Size: 6304
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2370 2671 3203 3503 4367 5751 6019
Size: 433 305 301 532 300 864 1384 268
Cuts at: 6019 1632
Size: 1917
Fragments arranged by size:
1917 1384 864 532 433 305 301 300
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 2383 2655 2907 5203 6180 1203
Size: 1180 272 252 2296 977 1327
Fragments arranged by size:
2296 1327 1180 977 272 252
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AlwNI CAG_nnn'CTG
Cuts at: 4047 5619 4047
Size: 1572 4732
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 2212 3145 3613 3839 3991 6100 1493
Size: 719 933 468 226 152 2109 1697
Fragments arranged by size:
2109 1697 933 719 468 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2279 3080 4858 160
Size: 872 1247 801 1778 1606
Fragments arranged by size:
1778 1606 1247 872 801
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 3462 3629 1870
Size: 73 726 793 167 4545
Fragments arranged by size:
4545 793 726 167 73
BamHI G'GATC_C
Cuts at: 2009 3634 4001 2009
Size: 1625 367 4312
Fragments arranged by size:
4312 1625 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 3625 5080 618
Size: 325 217 627 1532 306 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2628 3623 1391
Size: 1237 995 4072
Fragments arranged by size:
4072 1237 995
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3527 928
Size: 971 1628 3705
Fragments arranged by size:
3705 1628 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 3072 1548
Size: 1524 4780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 2639 3721 2639
Size: 1082 5222
Bme1580I G_kGCm'C
Cuts at: 1790 4365 5521 1790
Size: 2575 1156 2573
Fragments arranged by size:
2575 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4035 4993 442
Size: 416 53 83 399 24 2618 958 1753
Fragments arranged by size:
2618 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2851 2332
Size: 519 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2083 2556 2669 2807 2987 4964 1239
Size: 766 78 473 113 138 180 1977 2579
Fragments arranged by size:
2579 1977 766 473 180 138 113 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2302 4429 5294 5592 2302
Size: 2127 865 298 3014
Fragments arranged by size:
3014 2127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2191 2233 2570 4967 882
Size: 617 692 42 337 2397 2219
Fragments arranged by size:
2397 2219 692 617 337 42
BsaAI yAC'GTr
Cuts at: 493 1204 2910 4095 493
Size: 711 1706 1185 2702
Fragments arranged by size:
2702 1706 1185 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 2610 3498 3536
Size: 53 83 186 936 645 432 888 38
Cuts at: 3536 4111 4493 5128 275
Size: 575 382 635 1451
Fragments arranged by size:
1451 936 888 645 635 575 432 382
186 83 53 38
BsaWI w'CCGG_w
Cuts at: 4737 5135 5409 5556 5967 4737
Size: 398 274 147 411 5074
Fragments arranged by size:
5074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2642 2672 3011
Size: 30 165 30 678 30 531 30 339
Cuts at: 3011 3041 1178
Size: 30 4441
Fragments arranged by size:
4441 678 531 339 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 2468 2520 3179 3446 3491 1186
Size: 822 460 52 659 267 45 3999
Fragments arranged by size:
3999 822 659 460 267 52 45
BseYI C'CCAG_C
Cuts at: 5507 6003 5507
Size: 496 5808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5731
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 3754 4515 5543 1540
Size: 275 186 1291 462 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 462 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 3171 4365 4450 5521 2655
Size: 516 1194 85 1071 3438
Fragments arranged by size:
3438 1194 1071 516 85
BsmI GAATG_Cn'
Cuts at: 2064 2324 3815 3908 2064
Size: 260 1491 93 4460
Fragments arranged by size:
4460 1491 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2628 2655 3171 3623 4365 4450 5521
Size: 399 838 27 516 452 742 85 1071
Cuts at: 5521 1391
Size: 2174
Fragments arranged by size:
2174 1071 838 742 516 452 399 85
27
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 2571 3659 844
Size: 1446 281 1088 3489
Fragments arranged by size:
3489 1446 1088 281
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2132 4948 5135 1700
Size: 110 218 104 2816 187 2869
Fragments arranged by size:
2869 2816 218 187 110 104
BsrGI T'GTAC_A
Cuts at: 96 2796 96
Size: 2700 3604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 3107 794
Size: 1297 1016 3991
Fragments arranged by size:
3991 1297 1016
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 3097 2047
Size: 1050 5254
BstYI r'GATC_y
Cuts at: 1972 2009 2997 3057 3634 4001 4386 4403 5855
Size: 37 988 60 577 367 385 17 1452
Cuts at: 5855 5941 5953 6299 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 988 577 385 367 346 86
60 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6304
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2985 3423 513
Size: 552 939 177 804 438 3394
Fragments arranged by size:
3394 939 804 552 438 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2110 2553 487
Size: 683 56 884 443 4238
Fragments arranged by size:
4238 884 683 443 56
BtsI GCAGTG_nn'
Cuts at: 2770 2977 3820 4614 4634 2770
Size: 207 843 794 20 4440
Fragments arranged by size:
4440 843 794 207 20
ClaI AT'CG_AT
Cuts at: 1554 2055 1554
Size: 501 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2183 5311 809
Size: 507 867 3128 1802
Fragments arranged by size:
3128 1802 867 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 2463 2868
Size: 54 1425 50 275 18 417 216 405
Cuts at: 2868 3087 3751 4640 6269 8
Size: 219 664 889 1629 43
Fragments arranged by size:
1629 1425 889 664 417 405 275 219
216 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3422 4234 1353
Size: 2069 812 3423
Fragments arranged by size:
3423 2069 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2065 2083 2370 2556 2669 2671
Size: 393 373 60 18 287 186 113 2
Cuts at: 2671 2807 2987 3203 3503 4367 4964 5751 6019
Size: 136 180 216 300 864 597 787 268
Cuts at: 6019 1239
Size: 1524
Fragments arranged by size:
1524 864 787 597 393 373 300 287
268 216 186 180 136 113 60 18
2
EcoO109I rG'GnC_Cy
Cuts at: 2587 2587
Size: 6304
EcoRI G'AATT_C
Cuts at: 2212 3613 3991 2212
Size: 1401 378 4525
Fragments arranged by size:
4525 1401 378
EcoRV GAT'ATC
Cuts at: 2848 3211 2848
Size: 363 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2620 2095
Size: 525 5779
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4945 4977 5019 5051 1373
Size: 32 135 32 3373 32 42 32 2626
Fragments arranged by size:
3373 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 3648 3894 669
Size: 988 281 588 1122 246 3079
Fragments arranged by size:
3079 1122 988 588 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3629 3629
Size: 6304
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 2383 6180 2383
Size: 3797 2507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3054 3085 5418 5602 1305
Size: 1749 31 2333 184 2007
Fragments arranged by size:
2333 2007 1749 184 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 3089 10
Size: 54 1425 343 417 840 3225
Fragments arranged by size:
3225 1425 840 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3095 4262 4621 4780 518
Size: 648 1929 1167 359 159 2042
Fragments arranged by size:
2042 1929 1167 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2867 2965 3720 4397 5545 5790 1606
Size: 1261 98 755 677 1148 245 2120
Fragments arranged by size:
2120 1261 1148 755 677 245 98
NcoI C'CATG_G
Cuts at: 513 1065 2004 2181 3423 513
Size: 552 939 177 1242 3394
Fragments arranged by size:
3394 1242 939 552 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 2659 3110 3662 5207 1132
Size: 97 1430 451 552 1545 2229
Fragments arranged by size:
2229 1545 1430 552 451 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 2655 5203 2655
Size: 2548 3756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 3044 1108
Size: 1776 160 4368
Fragments arranged by size:
4368 1776 160
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2819 3447 3656 830
Size: 1989 628 209 3478
Fragments arranged by size:
3478 1989 628 209
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
RsrII CG'GwC_CG
Cuts at: 2941 2941
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SapI GCTCTTCn'nnn_
Cuts at: 3422 3422
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 2090 4552 6264 2090
Size: 2462 1712 2130
Fragments arranged by size:
2462 2130 1712
SfcI C'TryA_G
Cuts at: 826 1045 2740 2815 3154 3443 3652 4787 5468
Size: 219 1695 75 339 289 209 1135 681
Cuts at: 5468 5659 826
Size: 191 1471
Fragments arranged by size:
1695 1471 1135 681 339 289 219 209
191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2317 4408 5309 5571 820
Size: 197 926 374 2091 901 262 1553
Fragments arranged by size:
2091 1553 926 901 374 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 3275 4228 5
Size: 47 3223 953 2081
Fragments arranged by size:
3223 2081 953 47
StuI AGG'CCT
Cuts at: 1517 3309 1517
Size: 1792 4512
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2181 3248 3348 3423 3743
Size: 552 939 30 147 1067 100 75 320
Cuts at: 3743 513
Size: 3074
Fragments arranged by size:
3074 1067 939 552 320 147 100 75
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3241 3348 4331 4348 4501 4686 5131
Size: 1255 1661 107 983 17 153 185 445
Cuts at: 5131 325
Size: 1498
Fragments arranged by size:
1661 1498 1255 983 445 185 153 107
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2496 2796
Size: 274 80 33 51 1443 111 408 300
Cuts at: 2796 4550 6262 96
Size: 1754 1712 138
Fragments arranged by size:
1754 1712 1443 408 300 274 138 111
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2391 2423 6264 6296 2391
Size: 32 3841 32 2399
Fragments arranged by size:
3841 2399 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2107
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 3309 4433 3309
Size: 1124 5180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2611 3537 4112 276
Size: 53 83 186 936 1077 926 575 2468
Fragments arranged by size:
2468 1077 936 926 575 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI RsrII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI