Restriction map for
pFN21AB9912
(Circular) (Six-base) MAPSORT of: pFN21AB9912.seq Check: 7554 from: 1 to: 6481
pFN21AB9912 6481 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 2497 3051 3162 4544 5928 6196 1632
Size: 837 28 554 111 1382 1384 268 1917
Fragments arranged by size:
1917 1384 1382 837 554 268 111 28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 2650 5210 2650
Size: 2560 3921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2879 2960 4224 5796 2879
Size: 81 1264 1572 3564
Fragments arranged by size:
3564 1572 1264 81
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2614 3790 4016 4168 6277 1493
Size: 1121 1176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3806 1870
Size: 73 1863 4545
Fragments arranged by size:
4545 1863 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3758 3791 3758
Size: 33 6448
BamHI G'GATC_C
Cuts at: 2818 3055 3811 4178 2818
Size: 237 756 367 5121
Fragments arranged by size:
5121 756 367 237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2536 3802 5257 618
Size: 325 217 627 333 416 1266 1455 1842
Fragments arranged by size:
1842 1455 1266 627 416 333 325 217
BanII G_rGCy'C
Cuts at: 1391 3800 1391
Size: 2409 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 4672 4706 1903
Size: 34 1586 34 1115 34 3678
Fragments arranged by size:
3678 1586 1115 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2718 4373 5583 2718
Size: 1655 1210 3616
Fragments arranged by size:
3616 1655 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3333 6476 3333
Size: 3143 3338
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2058 2655 3036
Size: 416 53 83 399 24 641 597 381
Cuts at: 3036 4212 5170 442
Size: 1176 958 1753
Fragments arranged by size:
1753 1176 958 641 597 416 399 381
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3712 3739 3791 5141 1239
Size: 2473 27 52 1350 2579
Fragments arranged by size:
2579 2473 1350 52 27
Bpu10I CC'TnA_GC
Cuts at: 3200 3200
Size: 6481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2987 4606 5471 5769 2987
Size: 1619 865 298 3699
Fragments arranged by size:
3699 1619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2382 2915 4272 493
Size: 711 1178 533 1357 2702
Fragments arranged by size:
2702 1357 1178 711 533
BsaBI GATnn'nnATC
Cuts at: 2880 2961 6475 2880
Size: 81 3514 2886
Fragments arranged by size:
3514 2886 81
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4914 5312 5586 5733 6144 4914
Size: 398 274 147 411 5251
Fragments arranged by size:
5251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2206 2236 2280 2310 3660
Size: 30 165 30 803 30 44 30 1350
Cuts at: 3660 3690 1178
Size: 30 3969
Fragments arranged by size:
3969 1350 803 165 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6481
BseYI C'CCAG_C
Cuts at: 3523 5684 6180 3523
Size: 2161 496 3824
Fragments arranged by size:
3824 2161 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3737 1163
Size: 573 2001 3907
Fragments arranged by size:
3907 2001 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4542 4627 5698 4542
Size: 85 1071 5325
Fragments arranged by size:
5325 1071 85
BsiWI C'GTAC_G
Cuts at: 2403 2403
Size: 6481
BsmI GAATG_Cn'
Cuts at: 2361 3992 4085 2361
Size: 1631 93 4757
Fragments arranged by size:
4757 1631 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3800 4542 4627 5698 1391
Size: 399 2010 742 85 1071 2174
Fragments arranged by size:
2174 2010 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3836 844
Size: 2992 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3248 3709 794
Size: 2454 461 3566
Fragments arranged by size:
3566 2454 461
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 2017 2818 3055 3333 3723 3811 4178 4563
Size: 45 801 237 278 390 88 367 385
Cuts at: 4563 4580 6032 6118 6130 6476 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 801 390 385 367 346 278
237 88 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3722 487
Size: 683 56 2496 3246
Fragments arranged by size:
3246 2496 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 3997 4791 4811 2158
Size: 1839 794 20 3828
Fragments arranged by size:
3828 1839 794 20
ClaI AT'CG_AT
Cuts at: 1554 2884 1554
Size: 1330 5151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 2187 3785 2187
Size: 1598 4883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2668 3928 4817
Size: 54 1425 50 275 18 838 1260 889
Cuts at: 4817 6446 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1260 889 838 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4411 1353
Size: 3058 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2497 3051 3162 3712 3739 3791
Size: 393 837 28 554 111 550 27 52
Cuts at: 3791 4544 5141 5928 6196 1239
Size: 753 597 787 268 1524
Fragments arranged by size:
1524 837 787 753 597 554 550 393
268 111 52 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3718 3718
Size: 6481
EcoO109I rG'GnC_Cy
Cuts at: 3030 3196 3030
Size: 166 6315
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2636 2668 2894 2926 2964
Size: 32 135 32 1064 32 226 32 38
Cuts at: 2964 2996 5122 5154 5196 5228 1373
Size: 32 2126 32 42 32 2626
Fragments arranged by size:
2626 2126 1064 226 135 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3825 4071 669
Size: 988 281 1503 384 246 3079
Fragments arranged by size:
3079 1503 988 384 281 246
HindIII A'AGCT_T
Cuts at: 748 2509 748
Size: 1761 4720
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 2208 4080 2208
Size: 1872 4609
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 3340 5595 5779 1305
Size: 993 1042 2255 184 2007
Fragments arranged by size:
2255 2007 1042 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2670 10
Size: 54 1425 343 838 3821
Fragments arranged by size:
3821 1425 838 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2354 3165 4439 4798 4957 518
Size: 648 843 345 811 1274 359 159 2042
Fragments arranged by size:
2042 1274 843 811 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 3897 4574 5722 5967 1606
Size: 2291 677 1148 245 2120
Fragments arranged by size:
2291 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 2329 2419 387
Size: 1942 90 4449
Fragments arranged by size:
4449 1942 90
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3839 5384 1132
Size: 97 2610 1545 2229
Fragments arranged by size:
2610 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3763 1108
Size: 2655 3826
PfoI T'CCnGG_A
Cuts at: 3476 3476
Size: 6481
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PmlI CAC'GTG
Cuts at: 2915 2915
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 4550 4582 6063 6095 2960
Size: 32 1558 32 1481 32 3346
Fragments arranged by size:
3346 1558 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3030 3196 3030
Size: 166 6315
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 2694 4051 2694
Size: 1357 5124
PstI C_TGCA'G
Cuts at: 830 3241 3402 3833 830
Size: 2411 161 431 3478
Fragments arranged by size:
3478 2411 431 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SexAI A'CCwGG_T
Cuts at: 3777 3777
Size: 6481
SfcI C'TryA_G
Cuts at: 826 1045 2367 3237 3316 3398 3829 4964 5645
Size: 219 1322 870 79 82 431 1135 681
Cuts at: 5645 5836 826
Size: 191 1471
Fragments arranged by size:
1471 1322 1135 870 681 431 219 191
82 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2966 4585 5486 5748 820
Size: 197 926 1023 1619 901 262 1553
Fragments arranged by size:
1619 1553 1023 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 3496 3580 4405 5
Size: 47 3444 84 825 2081
Fragments arranged by size:
3444 2081 825 84 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 2004 3076 3762 3920 513
Size: 552 939 1072 686 158 3074
Fragments arranged by size:
3074 1072 939 686 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2875 3076 3443 4508 4525 4678 4863
Size: 1255 1295 201 367 1065 17 153 185
Cuts at: 4863 5308 325
Size: 445 1498
Fragments arranged by size:
1498 1295 1255 1065 445 367 201 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4727 6439
Size: 274 80 33 51 1443 855 1895 1712
Cuts at: 6439 96
Size: 138
Fragments arranged by size:
1895 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 2899 2931 3472 3504 3633 3665 6441
Size: 32 587 32 541 32 129 32 2776
Cuts at: 6441 6473 2280
Size: 32 2288
Fragments arranged by size:
2776 2288 587 541 129 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3155 1316
Size: 1839 4642
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 2602 4610 2602
Size: 2008 4473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI