Restriction map for
pFN21AB9910
(Circular) (Six-base) MAPSORT of: pFN21AB9910.seq Check: 6523 from: 1 to: 6475
pFN21AB9910 6475 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3392 3519 3392
Size: 127 6348
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3624 4285 278
Size: 53 83 186 936 2088 661 2468
Fragments arranged by size:
2468 2088 936 661 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 2553 3695 3796 2553
Size: 1142 101 5232
Fragments arranged by size:
5232 1142 101
AclI AA'CG_TT
Cuts at: 1563 3774 4602 4975 1563
Size: 2211 828 373 3063
Fragments arranged by size:
3063 2211 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2436 2514 3173 3308 4538 5922 6190
Size: 590 214 78 659 135 1230 1384 268
Cuts at: 6190 1632
Size: 1917
Fragments arranged by size:
1917 1384 1230 659 590 268 214 135
78
AfeI AGC'GCT
Cuts at: 2804 2804
Size: 6475
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 2712 5374 6351 1203
Size: 1509 2662 977 1327
Fragments arranged by size:
2662 1509 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 2270 2573 3611 1166
Size: 1104 303 1038 4030
Fragments arranged by size:
4030 1104 1038 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2823 1184
Size: 1639 4836
AlwNI CAG_nnn'CTG
Cuts at: 2880 3384 3397 3412 4218 5790 2880
Size: 504 13 15 806 1572 3565
Fragments arranged by size:
3565 1572 806 504 15 13
ApaLI G'TGCA_C
Cuts at: 4532 5688 4532
Size: 1156 5319
ApoI r'AATT_y
Cuts at: 1493 2153 3784 4010 4162 6271 1493
Size: 660 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 2608 2891 3800 1870
Size: 73 220 445 283 909 4545
Fragments arranged by size:
4545 909 445 283 220 73
BamHI G'GATC_C
Cuts at: 3805 4172 3805
Size: 367 6108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 2553 3695 3796 5251
Size: 325 217 627 237 529 1142 101 1455
Cuts at: 5251 618
Size: 1842
Fragments arranged by size:
1842 1455 1142 627 529 325 237 217
101
BanII G_rGCy'C
Cuts at: 1391 2771 2917 3043 3794 1391
Size: 1380 146 126 751 4072
Fragments arranged by size:
4072 1380 751 146 126
BbeI G_GCGC'C
Cuts at: 2028 2028
Size: 6475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3491 928
Size: 971 1592 3912
Fragments arranged by size:
3912 1592 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2885 5086 136
Size: 107 122 71 631 605 1213 2201 1525
Fragments arranged by size:
2201 1525 1213 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6470 6470
Size: 6475
BlpI GC'TnA_GC
Cuts at: 3892 3892
Size: 6475
Bme1580I G_kGCm'C
Cuts at: 1790 2858 4536 5692 1790
Size: 1068 1678 1156 2573
Fragments arranged by size:
2573 1678 1156 1068
BmgBI CAC'GTC
Cuts at: 1318 3460 1318
Size: 2142 4333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4206 5164 442
Size: 416 53 83 399 24 2789 958 1753
Fragments arranged by size:
2789 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2214 3584 3695 5135 1239
Size: 888 87 1370 111 1440 2579
Fragments arranged by size:
2579 1440 1370 888 111 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3494 4600 5465 5763 3494
Size: 1106 865 298 4206
Fragments arranged by size:
4206 1106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2232 2420 3101 5138 882
Size: 617 733 188 681 2037 2219
Fragments arranged by size:
2219 2037 733 681 617 188
BsaAI yAC'GTr
Cuts at: 493 1204 2466 2715 4266 493
Size: 711 1262 249 1551 2702
Fragments arranged by size:
2702 1551 1262 711 249
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2025 2829 3621 4282
Size: 53 83 186 936 492 804 792 661
Cuts at: 4282 4664 5299 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 804 792 661 635 492 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2619 2974 4908 5306 5580 5727 6138 2619
Size: 355 1934 398 274 147 411 2956
Fragments arranged by size:
2956 1934 411 398 355 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 3648 3678 1178
Size: 30 165 30 1296 30 919 30 3975
Fragments arranged by size:
3975 1296 919 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2664 2771 2961 3621 1186
Size: 1478 107 190 660 4040
Fragments arranged by size:
4040 1478 660 190 107
BseYI C'CCAG_C
Cuts at: 2431 3450 5678 6174 2431
Size: 1019 2228 496 2732
Fragments arranged by size:
2732 2228 1019 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2819 3383 1163
Size: 573 615 468 564 4255
Fragments arranged by size:
4255 615 573 564 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3925 4686 5714 1540
Size: 275 186 60 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2619 2712 3242 3447 4536 4621 5692 2619
Size: 93 530 205 1089 85 1071 3402
Fragments arranged by size:
3402 1089 1071 530 205 93 85
BsiWI C'GTAC_G
Cuts at: 3087 3087
Size: 6475
BsmI GAATG_Cn'
Cuts at: 2147 3986 4079 2147
Size: 1839 93 4543
Fragments arranged by size:
4543 1839 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2619 2712 2771 2858 2917 3043 3242
Size: 399 829 93 59 87 59 126 199
Cuts at: 3242 3447 3794 4536 4621 5692 1391
Size: 205 347 742 85 1071 2174
Fragments arranged by size:
2174 1071 829 742 399 347 205 199
126 93 87 85 59 59
BspEI T'CCGG_A
Cuts at: 2619 6138 2619
Size: 3519 2956
BspHI T'CATG_A
Cuts at: 2824 4364 6094 2824
Size: 1540 1730 3205
Fragments arranged by size:
3205 1730 1540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2323 3392 3519 3683 3830 844
Size: 1454 25 1069 127 164 147 3489
Fragments arranged by size:
3489 1454 1069 164 147 127 25
BsrBI CCG'CTC
Cuts at: 1394 1823 4362 1394
Size: 429 2539 3507
Fragments arranged by size:
3507 2539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3282 3624 4970 5144 65
Size: 1183 2034 342 1346 174 1396
Fragments arranged by size:
2034 1396 1346 1183 342 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5119 5306 1700
Size: 110 3309 187 2869
Fragments arranged by size:
3309 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 4535 5547 4535
Size: 1012 5463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 3805 4172 4557 4574 6026 6112 6124 6470
Size: 1833 367 385 17 1452 86 12 346
Cuts at: 6470 1972
Size: 1977
Fragments arranged by size:
1977 1833 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2701 513
Size: 552 939 697 4287
Fragments arranged by size:
4287 939 697 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3673 487
Size: 683 56 2447 3289
Fragments arranged by size:
3289 2447 683 56
BtsI GCAGTG_nn'
Cuts at: 3488 3745 3991 4785 4805 3488
Size: 257 246 794 20 5158
Fragments arranged by size:
5158 794 257 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3031 3779 3031
Size: 748 5727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2936 5482 809
Size: 507 1620 2546 1802
Fragments arranged by size:
2546 1802 1620 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2086 2295
Size: 54 1425 50 275 18 228 28 209
Cuts at: 2295 3922 4811 6440 8
Size: 1627 889 1629 43
Fragments arranged by size:
1629 1627 1425 889 275 228 209 54
50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 3922 1537
Size: 275 246 1864 4090
Fragments arranged by size:
4090 1864 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2357 4405 1353
Size: 845 159 2048 3423
Fragments arranged by size:
3423 2048 845 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5062 5436 5582 387
Size: 1064 138 3473 374 146 1280
Fragments arranged by size:
3473 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2214 2222 2436 2514 3173 3308
Size: 393 495 87 8 214 78 659 135
Cuts at: 3308 3584 3695 4538 5135 5922 6190 1239
Size: 276 111 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 659 597 495 393 276
268 214 135 111 87 78 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2872 2872
Size: 6475
EcoO109I rG'GnC_Cy
Cuts at: 2473 2877 3213 3409 2473
Size: 404 336 196 5539
Fragments arranged by size:
5539 404 336 196
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2678 2678
Size: 6475
FspI TGC'GCA
Cuts at: 2780 4981 2780
Size: 2201 4274
HaeII r_GCGC'y
Cuts at: 1678 2028 2806 5622 1678
Size: 350 778 2816 2531
Fragments arranged by size:
2816 2531 778 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3120 3140 3152 3172 5116
Size: 32 135 32 1548 20 12 20 1944
Cuts at: 5116 5148 5190 5222 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1944 1548 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3819 4065 669
Size: 988 281 1221 660 246 3079
Fragments arranged by size:
3079 1221 988 660 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 3428 748
Size: 1786 894 3795
Fragments arranged by size:
3795 1786 894
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KasI G'GCGC_C
Cuts at: 2024 2024
Size: 6475
KpnI G_GTAC'C
Cuts at: 2557 3699 3800 2557
Size: 1142 101 5232
Fragments arranged by size:
5232 1142 101
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5589 5773 1305
Size: 4284 184 2007
Fragments arranged by size:
4284 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4653
Fragments arranged by size:
4653 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2573 2960 3611 3767 4433 4792
Size: 648 1104 303 387 651 156 666 359
Cuts at: 4792 4951 518
Size: 159 2042
Fragments arranged by size:
2042 1104 666 651 648 387 359 303
159 156
MspA1I CmG'CkG
Cuts at: 1606 2270 2774 2861 3331 3418 3454 3891 4568
Size: 664 504 87 470 87 36 437 677
Cuts at: 4568 5716 5961 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 504 470 437 245
87 87 36
NarI GG'CG_CC
Cuts at: 2025 2025
Size: 6475
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4984
Fragments arranged by size:
4984 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2589 3833 5378 1132
Size: 97 1360 1244 1545 2229
Fragments arranged by size:
2229 1545 1360 1244 97
PasI CC'CwG_GG
Cuts at: 1348 2874 1348
Size: 1526 4949
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3082 1108
Size: 1974 4501
PfoI T'CCnGG_A
Cuts at: 3003 3672 3003
Size: 669 5806
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PmlI CAC'GTG
Cuts at: 2466 2466
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 3409 2473
Size: 936 5539
PshAI GACnn'nnGTC
Cuts at: 1613 2832 1613
Size: 1219 5256
PsiI TTA'TAA
Cuts at: 2484 4045 2484
Size: 1561 4914
PstI C_TGCA'G
Cuts at: 830 3336 3417 3827 830
Size: 2506 81 410 3478
Fragments arranged by size:
3478 2506 410 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2861 3331 3418 3454 2861
Size: 470 87 36 5882
Fragments arranged by size:
5882 470 87 36
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 3332 3413 3823 4958 5639 5830 826
Size: 219 2287 81 410 1135 681 191 1471
Fragments arranged by size:
2287 1471 1135 681 410 219 191 81
SfoI GGC'GCC
Cuts at: 2026 2026
Size: 6475
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 2165 3802 1872
Size: 293 1637 4545
Fragments arranged by size:
4545 1637 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2608 3473 4579 5480 5742 820
Size: 197 926 665 865 1106 901 262 1553
Fragments arranged by size:
1553 1106 926 901 865 665 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 3223 152
Size: 3071 3404
SphI G_CATG'C
Cuts at: 1132 2589 3833 1132
Size: 1457 1244 3774
Fragments arranged by size:
3774 1457 1244
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6475
StyI C'CwwG_G
Cuts at: 513 1065 2004 2850 3317 3914 513
Size: 552 939 846 467 597 3074
Fragments arranged by size:
3074 939 846 597 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3269 3283 4502 4519 4672 4857 5302
Size: 1255 1689 14 1219 17 153 185 445
Cuts at: 5302 325
Size: 1498
Fragments arranged by size:
1689 1498 1255 1219 445 185 153 17
14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3529 4721 6433
Size: 274 80 33 51 1443 1552 1192 1712
Cuts at: 6433 96
Size: 138
Fragments arranged by size:
1712 1552 1443 1192 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 2608 1943
Size: 665 5810
XmaI C'CCGG_G
Cuts at: 1870 2163 3800 1870
Size: 293 1637 4545
Fragments arranged by size:
4545 1637 293
XmnI GAAnn'nnTTC
Cuts at: 3180 4604 3180
Size: 1424 5051
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3622 4283 276
Size: 53 83 186 936 2088 661 2468
Fragments arranged by size:
2468 2088 936 661 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI