Restriction map for
pFN21AB9909
(Circular) (Six-base) MAPSORT of: pFN21AB9909.seq Check: 94 from: 1 to: 5473
pFN21AB9909 5473 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3536 4920 5188 1632
Size: 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AlwNI CAG_nnn'CTG
Cuts at: 2242 2461 2512 3216 4788 2242
Size: 219 51 704 1572 2927
Fragments arranged by size:
2927 1572 704 219 51
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2155 2798 1870
Size: 73 130 82 643 4545
Fragments arranged by size:
4545 643 130 82 73
BamHI G'GATC_C
Cuts at: 2730 2803 3170 2730
Size: 73 367 5033
Fragments arranged by size:
5033 367 73
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2450 2486 2794 4249
Size: 325 217 627 390 273 36 308 1455
Cuts at: 4249 618
Size: 1842
Fragments arranged by size:
1842 1455 627 390 325 308 273 217
36
BanII G_rGCy'C
Cuts at: 1391 2266 2289 2523 2792 1391
Size: 875 23 234 269 4072
Fragments arranged by size:
4072 875 269 234 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2584 2618 3664 3698 1903
Size: 34 647 34 1046 34 3678
Fragments arranged by size:
3678 1046 647 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4084 136
Size: 107 122 71 631 605 2412 1525
Fragments arranged by size:
2412 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2060 2375 2890 2060
Size: 315 515 4643
Fragments arranged by size:
4643 515 315
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2445 2491 3534 4690 1790
Size: 310 345 46 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 345 310 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2449 3204 4162
Size: 416 53 83 399 24 1032 755 958
Cuts at: 4162 442
Size: 1753
Fragments arranged by size:
1753 1032 958 755 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4133 1239
Size: 2894 2579
Bpu10I CC'TnA_GC
Cuts at: 2313 2313
Size: 5473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 4463 4761 3598
Size: 865 298 4310
Fragments arranged by size:
4310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3264 493
Size: 711 1518 542 2702
Fragments arranged by size:
2702 1518 711 542
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 3280 3662 4297
Size: 53 83 186 936 503 1244 382 635
Cuts at: 4297 275
Size: 1451
Fragments arranged by size:
1451 1244 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4504
Fragments arranged by size:
4504 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 1186
Size: 1168 4305
BseYI C'CCAG_C
Cuts at: 2021 2192 2235 2292 2383 2472 2500 4676 5172
Size: 171 43 57 91 89 28 2176 496
Cuts at: 5172 2021
Size: 2322
Fragments arranged by size:
2322 2176 496 171 91 89 57 43
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4900
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2211 2923 3684 4712 1540
Size: 275 186 210 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 210 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2523 3534 3619 4690 2259
Size: 264 1011 85 1071 3042
Fragments arranged by size:
3042 1071 1011 264 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2259 2266 2289 2445 2491 2523
Size: 399 310 159 7 23 156 46 32
Cuts at: 2523 2792 3534 3619 4690 1391
Size: 269 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 269 159 156
85 46 32 23 7
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 3360 1394
Size: 429 241 1296 3507
Fragments arranged by size:
3507 1296 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 2550 2591 4117 4304 1700
Size: 110 203 537 41 1526 187 2869
Fragments arranged by size:
2869 1526 537 203 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 2099 3533 4545 2099
Size: 1434 1012 3027
Fragments arranged by size:
3027 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 2457 794
Size: 1427 236 3810
Fragments arranged by size:
3810 1427 236
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 2536 1184
Size: 1352 4121
BstYI r'GATC_y
Cuts at: 1972 2730 2803 3170 3555 3572 5024 5110 5122
Size: 758 73 367 385 17 1452 86 12
Cuts at: 5122 5468 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2446 2466 2532 513
Size: 552 939 442 20 66 3454
Fragments arranged by size:
3454 939 552 442 66 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2215 2989 3783 3803 2215
Size: 774 794 20 3885
Fragments arranged by size:
3885 794 774 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DraIII CAC_nnn'GTG
Cuts at: 2272 2457 2272
Size: 185 5288
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2463 2552
Size: 54 1425 50 275 18 378 255 89
Cuts at: 2552 2920 3809 5438 8
Size: 368 889 1629 43
Fragments arranged by size:
1629 1425 889 378 368 275 255 89
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2208 2920 1537
Size: 275 396 712 4090
Fragments arranged by size:
4090 712 396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3403 1353
Size: 2050 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 4434 4580 387
Size: 1064 138 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2521 2521
Size: 5473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3536 4133 4920 5188 1239
Size: 393 1904 597 787 268 1524
Fragments arranged by size:
1904 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2509 2509
Size: 5473
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 2242 4620 1678
Size: 564 2378 2531
Fragments arranged by size:
2531 2378 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373
Size: 32 135 32 2542 32 42 32 2626
Fragments arranged by size:
2626 2542 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 10
Size: 54 1425 343 633 3018
Fragments arranged by size:
3018 1425 633 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3431 3790 3949 518
Size: 648 2265 359 159 2042
Fragments arranged by size:
2265 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2374 2419 2889 3566 4714 4959 1606
Size: 768 45 470 677 1148 245 2120
Fragments arranged by size:
2120 1148 768 677 470 245 45
NaeI GCC'GGC
Cuts at: 2552 2593 2552
Size: 41 5432
NcoI C'CATG_G
Cuts at: 513 1065 2004 2466 513
Size: 552 939 462 3520
Fragments arranged by size:
3520 939 552 462
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NgoMIV G'CCGG_C
Cuts at: 2550 2591 2550
Size: 41 5432
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2831 4376 1132
Size: 97 1602 1545 2229
Fragments arranged by size:
2229 1602 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2461 2472 1108
Size: 1134 219 11 4109
Fragments arranged by size:
4109 1134 219 11
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 2509
Size: 5473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2142 2517 2825 830
Size: 1312 375 308 3478
Fragments arranged by size:
3478 1312 375 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2374 2419 2374
Size: 45 5428
SacI G_AGCT'C
Cuts at: 2523 2523
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2517 2825 2517
Size: 308 5165
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2138 2513 2821 3956 4637 4828 826
Size: 219 1093 375 308 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 375 308 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2157 2800 1872
Size: 285 643 4545
Fragments arranged by size:
4545 643 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 4478 4740 820
Size: 197 926 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5473
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 2644 2760 2912 513
Size: 552 939 462 178 116 152 3074
Fragments arranged by size:
3074 939 552 462 178 152 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 2468 2474 3500 3517 3670 3855
Size: 1255 726 162 6 1026 17 153 185
Cuts at: 3855 4300 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1026 726 445 185 162 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3719 5431 96
Size: 274 80 33 51 1443 1742 1712 138
Fragments arranged by size:
1742 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5433 5465 5433
Size: 32 5441
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2155 2798 1870
Size: 285 643 4545
Fragments arranged by size:
4545 643 285
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI