Restriction map for
pFN21AB9908
(Circular) (Six-base) MAPSORT of: pFN21AB9908.seq Check: 7329 from: 1 to: 6472
pFN21AB9908 6472 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3643 3643
Size: 6472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2545 2960 3655 4535 5919 6187 1632
Size: 913 415 695 880 1384 268 1917
Fragments arranged by size:
1917 1384 913 880 695 415 268
AfeI AGC'GCT
Cuts at: 3236 3236
Size: 6472
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 3090 5371 6348 1203
Size: 1887 2281 977 1327
Fragments arranged by size:
2281 1887 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6472
AlwNI CAG_nnn'CTG
Cuts at: 2401 2716 3574 4215 5787 2401
Size: 315 858 641 1572 3086
Fragments arranged by size:
3086 1572 858 641 315
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2326 3781 4007 4159 6268 1493
Size: 833 1455 226 152 2109 1697
Fragments arranged by size:
2109 1697 1455 833 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2049 2150 2945 3014 3218 3746
Size: 73 69 37 101 795 69 204 528
Cuts at: 3746 3797 1870
Size: 51 4545
Fragments arranged by size:
4545 795 528 204 101 73 69 69
51 37
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 3044 3793 5248 618
Size: 325 217 627 406 851 749 1455 1842
Fragments arranged by size:
1842 1455 851 749 627 406 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3722 3791 1391
Size: 622 1709 69 4072
Fragments arranged by size:
4072 1709 622 69
BbeI G_GCGC'C
Cuts at: 2197 2197
Size: 6472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2863 928
Size: 971 964 4537
Fragments arranged by size:
4537 971 964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2017 2051 3132 3166 4663 4697 1903
Size: 34 80 34 1081 34 1497 34 3678
Fragments arranged by size:
3678 1497 1081 80 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2191 2847 5083
Size: 107 122 71 631 605 519 656 2236
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
2236 1525 656 631 605 519 122 107
71
BglII A'GATC_T
Cuts at: 2886 6467 2886
Size: 3581 2891
BlpI GC'TnA_GC
Cuts at: 2144 2234 3889 2144
Size: 90 1655 4727
Fragments arranged by size:
4727 1655 90
Bme1580I G_kGCm'C
Cuts at: 1790 3015 3047 3322 3528 4533 5689 1790
Size: 1225 32 275 206 1005 1156 2573
Fragments arranged by size:
2573 1225 1156 1005 275 206 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5161 442
Size: 416 53 83 399 24 2786 958 1753
Fragments arranged by size:
2786 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2423 2738 2921 3407 3497 5132 1239
Size: 851 333 315 183 486 90 1635 2579
Fragments arranged by size:
2579 1635 851 486 333 315 183 90
Bpu10I CC'TnA_GC
Cuts at: 2462 2462
Size: 6472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2801 4597 5462 5760 2027
Size: 774 1796 865 298 2739
Fragments arranged by size:
2739 1796 865 774 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2759 3548 5135 882
Size: 617 1260 789 1587 2219
Fragments arranged by size:
2219 1587 1260 789 617
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2194 4279 4661 5296
Size: 53 83 186 936 661 2085 382 635
Cuts at: 5296 275
Size: 1451
Fragments arranged by size:
2085 1451 936 661 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3064 4905 5303 5577 5724 6135 3064
Size: 1841 398 274 147 411 3401
Fragments arranged by size:
3401 1841 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3545 3575 1178
Size: 30 165 30 2142 30 4075
Fragments arranged by size:
4075 2142 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3203 3236 3263 1186
Size: 2017 33 27 4395
Fragments arranged by size:
4395 2017 33 27
BseYI C'CCAG_C
Cuts at: 2394 3370 3741 5675 6171 2394
Size: 976 371 1934 496 2695
Fragments arranged by size:
2695 1934 976 496 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 2894 3621 1163
Size: 573 638 520 727 4014
Fragments arranged by size:
4014 727 638 573 520
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3922 4683 5711 1540
Size: 275 186 1921 761 1028 2301
Fragments arranged by size:
2301 1921 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 3339 3722 4533 4618 5689 2013
Size: 1326 383 811 85 1071 2796
Fragments arranged by size:
2796 1326 1071 811 383 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 3015 3047 3322 3339 3528 3722
Size: 399 223 1002 32 275 17 189 194
Cuts at: 3722 3791 4533 4618 5689 1391
Size: 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 1002 742 399 275 223 194
189 85 69 32 17
BspEI T'CCGG_A
Cuts at: 3064 6135 3064
Size: 3071 3401
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2916 3643 3827 844
Size: 2072 727 184 3489
Fragments arranged by size:
3489 2072 727 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2179 3199 3255 4359 1394
Size: 429 356 1020 56 1104 3507
Fragments arranged by size:
3507 1104 1020 429 356 56
BsrDI GCAATG_nn'
Cuts at: 65 1248 4967 5141 65
Size: 1183 3719 174 1396
Fragments arranged by size:
3719 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5116 5303 1700
Size: 110 3306 187 2869
Fragments arranged by size:
3306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2928 96
Size: 2832 3640
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 2221 2453 4532 5544 2221
Size: 232 2079 1012 3149
Fragments arranged by size:
3149 2079 1012 232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 2712 2886 3802 4169 4554 4571 6023 6109
Size: 740 174 916 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 916 740 385 367 346 174
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2361 2361
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 2968 3048 513
Size: 552 939 687 277 80 3937
Fragments arranged by size:
3937 939 687 552 277 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 2525 3322 3526 3988 4782 4802 2525
Size: 797 204 462 794 20 4195
Fragments arranged by size:
4195 797 794 462 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3672 5479 809
Size: 507 2356 1807 1802
Fragments arranged by size:
2356 1807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2971 3919
Size: 54 1425 50 275 18 435 706 948
Cuts at: 3919 4808 6437 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 948 889 706 435 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3674 3710 4402 1353
Size: 774 1547 36 692 3423
Fragments arranged by size:
3423 1547 774 692 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5059 5433 5579 387
Size: 1064 138 3470 374 146 1280
Fragments arranged by size:
3470 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 3720 2011
Size: 1709 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2423 2545 2738 2921 2960 3407
Size: 393 458 333 122 193 183 39 447
Cuts at: 3407 3497 3655 4535 5132 5919 6187 1239
Size: 90 158 880 597 787 268 1524
Fragments arranged by size:
1524 880 787 597 458 447 393 333
268 193 183 158 122 90 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 3278 2429
Size: 849 5623
EcoO109I rG'GnC_Cy
Cuts at: 2345 2365 2411 2905 2935 3214 3283 3751 2345
Size: 20 46 494 30 279 69 468 5066
Fragments arranged by size:
5066 494 468 279 69 46 30 20
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2197 2699 3116 3238 5619 1678
Size: 519 502 417 122 2381 2531
Fragments arranged by size:
2531 2381 519 502 417 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5113 5145 5187 5219 1373
Size: 32 135 32 3541 32 42 32 2626
Fragments arranged by size:
3541 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KasI G'GCGC_C
Cuts at: 2193 2193
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5586 5770 1305
Size: 4281 184 2007
Fragments arranged by size:
4281 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4430 4789 4948 518
Size: 648 3264 359 159 2042
Fragments arranged by size:
3264 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2377 2398 3888 4565 5713 5958 1606
Size: 635 136 21 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 677 635 245 136 21
NarI GG'CG_CC
Cuts at: 2194 2194
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 2004 2691 513
Size: 552 939 687 4294
Fragments arranged by size:
4294 939 687 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 2307 3011 3385 3830 5375 1132
Size: 97 1078 704 374 445 1545 2229
Fragments arranged by size:
2229 1545 1078 704 445 374 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 3534 3765 1108
Size: 1293 1133 231 3815
Fragments arranged by size:
3815 1293 1133 231
PfoI T'CCnGG_A
Cuts at: 2253 2430 2715 3567 2253
Size: 177 285 852 5158
Fragments arranged by size:
5158 852 285 177
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2345 2365 2411 2905 2935 3214 3283 2345
Size: 20 46 494 30 279 69 5534
Fragments arranged by size:
5534 494 279 69 46 30 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspXI vC'TCGA_Gb
Cuts at: 2150 2150
Size: 6472
PstI C_TGCA'G
Cuts at: 830 2520 3663 3703 3824 830
Size: 1690 1143 40 121 3478
Fragments arranged by size:
3478 1690 1143 121 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2377 2377
Size: 6472
SacI G_AGCT'C
Cuts at: 2013 3722 2013
Size: 1709 4763
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SanDI GG'GwC_CC
Cuts at: 2411 2411
Size: 6472
SapI GCTCTTCn'nnn_
Cuts at: 2127 3710 2127
Size: 1583 4889
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2516 3659 3699 3820 4955 5636 5827
Size: 219 1471 1143 40 121 1135 681 191
Cuts at: 5827 826
Size: 1471
Fragments arranged by size:
1471 1471 1143 1135 681 219 191 121
40
SfoI GGC'GCC
Cuts at: 2195 2195
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 2051 3799 1872
Size: 179 1748 4545
Fragments arranged by size:
4545 1748 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 2042 2150 2816 4576 5477
Size: 197 926 69 30 108 666 1760 901
Cuts at: 5477 5739 820
Size: 262 1553
Fragments arranged by size:
1760 1553 926 901 666 262 197 108
69 30
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3385 3830 1132
Size: 2253 445 3774
Fragments arranged by size:
3774 2253 445
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2004 2691 2769 3911 513
Size: 552 939 687 78 1142 3074
Fragments arranged by size:
3074 1142 939 687 552 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 3572 4499 4516 4669 4854 5299
Size: 1255 828 1164 927 17 153 185 445
Cuts at: 5299 325
Size: 1498
Fragments arranged by size:
1498 1255 1164 927 828 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2928 4718 6430
Size: 274 80 33 51 1443 951 1790 1712
Cuts at: 6430 96
Size: 138
Fragments arranged by size:
1790 1712 1443 951 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2290 2322 2765 2797 2988 3020 3545 3577 6432
Size: 32 443 32 191 32 525 32 2855
Cuts at: 6432 6464 2290
Size: 32 2298
Fragments arranged by size:
2855 2298 525 443 191 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3615 3615
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 2012 2150 1943
Size: 69 138 6265
Fragments arranged by size:
6265 138 69
XmaI C'CCGG_G
Cuts at: 1870 2049 3797 1870
Size: 179 1748 4545
Fragments arranged by size:
4545 1748 179
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacII SexAI SfiI SrfI SwaI