Restriction map for
pFN21AB9904
(Circular) (Six-base) MAPSORT of: pFN21AB9904.seq Check: 6264 from: 1 to: 6472
pFN21AB9904 6472 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3300 3815 1937
Size: 1363 515 4594
Fragments arranged by size:
4594 1363 515
Acc65I G'GTAC_C
Cuts at: 2973 3793 2973
Size: 820 5652
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 3418 3758 4535 5919 6187 1632
Size: 1181 605 340 777 1384 268 1917
Fragments arranged by size:
1917 1384 1181 777 605 340 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3527 1184
Size: 2343 4129
AlwNI CAG_nnn'CTG
Cuts at: 3349 4215 5787 3349
Size: 866 1572 4034
Fragments arranged by size:
4034 1572 866
ApaI G_GGCC'C
Cuts at: 2176 2964 3009 3071 2176
Size: 788 45 62 5577
Fragments arranged by size:
5577 788 62 45
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2714 3242 3781 4007 4159 6268 1493
Size: 1221 528 539 226 152 2109 1697
Fragments arranged by size:
2109 1697 1221 539 528 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 3122 3797 1870
Size: 73 82 1097 675 4545
Fragments arranged by size:
4545 1097 675 82 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2973 3096 3793 5248 618
Size: 325 217 627 1186 123 697 1455 1842
Fragments arranged by size:
1842 1455 1186 697 627 325 217 123
BanII G_rGCy'C
Cuts at: 1391 2176 2964 3009 3071 3791 1391
Size: 785 788 45 62 720 4072
Fragments arranged by size:
4072 788 785 720 62 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3193 3202 3414 928
Size: 971 1294 9 212 3986
Fragments arranged by size:
3986 1294 971 212 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2839 4364 5574 2839
Size: 1525 1210 3737
Fragments arranged by size:
3737 1525 1210
BclI T'GATC_A
Cuts at: 1548 2839 1548
Size: 1291 5181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 2741 5083
Size: 107 122 71 631 605 577 492 2342
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
2342 1525 631 605 577 492 122 107
71
BglII A'GATC_T
Cuts at: 2538 3561 6467 2538
Size: 1023 2906 2543
Fragments arranged by size:
2906 2543 1023
BlpI GC'TnA_GC
Cuts at: 3759 3889 3759
Size: 130 6342
Bme1580I G_kGCm'C
Cuts at: 1790 2176 2737 2964 3009 3071 3099 4533 5689
Size: 386 561 227 45 62 28 1434 1156
Cuts at: 5689 1790
Size: 2573
Fragments arranged by size:
2573 1434 1156 561 386 227 62 45
28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 3082 4203
Size: 416 53 83 399 24 886 779 1121
Cuts at: 4203 5161 442
Size: 958 1753
Fragments arranged by size:
1753 1121 958 886 779 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3646 3646
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 3554 3665 3692 5132 1239
Size: 833 1482 111 27 1440 2579
Fragments arranged by size:
2579 1482 1440 833 111 27
Bpu10I CC'TnA_GC
Cuts at: 2178 2178
Size: 6472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 4597 5462 5760 2171
Size: 2426 865 298 2883
Fragments arranged by size:
2883 2426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 4279 4661 5296
Size: 53 83 186 936 508 2238 382 635
Cuts at: 5296 275
Size: 1451
Fragments arranged by size:
2238 1451 936 635 508 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 1178
Size: 30 165 30 682 30 5535
Fragments arranged by size:
5535 682 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2253 3560 3622 1186
Size: 1067 1307 62 4036
Fragments arranged by size:
4036 1307 1067 62
BseYI C'CCAG_C
Cuts at: 2506 2736 3500 3574 5675 6171 2506
Size: 230 764 74 2101 496 2807
Fragments arranged by size:
2807 2101 764 496 230 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3497 1163
Size: 573 1761 4138
Fragments arranged by size:
4138 1761 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2090 2170 3922 4683 5711 1540
Size: 275 186 89 80 1752 761 1028 2301
Fragments arranged by size:
2301 1752 1028 761 275 186 89 80
BsiHKAI G_wGCw'C
Cuts at: 3156 4533 4618 5689 3156
Size: 1377 85 1071 3939
Fragments arranged by size:
3939 1377 1071 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2737 2964 3009 3071 3099 3156
Size: 399 386 561 227 45 62 28 57
Cuts at: 3156 3791 4533 4618 5689 1391
Size: 635 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 561 399 386 227
85 62 57 45 28
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 3246 4361 6091 3246
Size: 1115 1730 3627
Fragments arranged by size:
3627 1730 1115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3827 844
Size: 2983 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 3232 4967 5141 65
Size: 1183 1245 739 1735 174 1396
Fragments arranged by size:
1735 1396 1245 1183 739 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 5116 5303 1700
Size: 110 391 2915 187 2869
Fragments arranged by size:
2915 2869 391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 2501 2885 3056 3538 1184
Size: 1317 384 171 482 4118
Fragments arranged by size:
4118 1317 482 384 171
BstYI r'GATC_y
Cuts at: 1972 2538 3561 3802 4169 4554 4571 6023 6109
Size: 566 1023 241 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1023 566 385 367 346 241
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2625 2965 3270 513
Size: 552 1560 340 305 3715
Fragments arranged by size:
3715 1560 552 340 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2111 2720 3582 3678 487
Size: 683 56 885 609 862 96 3281
Fragments arranged by size:
3281 885 862 683 609 96 56
BtsI GCAGTG_nn'
Cuts at: 2164 2469 3753 3988 4782 4802 2164
Size: 305 1284 235 794 20 3834
Fragments arranged by size:
3834 1284 794 305 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2857 2892 529
Size: 35 2293 35 4109
Fragments arranged by size:
4109 2293 35 35
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2167 3587
Size: 54 1425 50 275 18 307 30 1420
Cuts at: 3587 3919 4808 6437 8
Size: 332 889 1629 43
Fragments arranged by size:
1629 1425 1420 889 332 307 275 54
50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3919 1537
Size: 275 355 1752 4090
Fragments arranged by size:
4090 1752 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4402 1353
Size: 3049 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 2139 5059 5433 5579 387
Size: 1064 138 456 94 2920 374 146 1280
Fragments arranged by size:
2920 1280 1064 456 374 146 138 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2813 3418 3554 3665 3692 3758
Size: 393 440 741 605 136 111 27 66
Cuts at: 3758 4535 5132 5919 6187 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 741 605 597 440 393
268 136 111 66 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 6472
EcoO109I rG'GnC_Cy
Cuts at: 2173 2326 2960 2961 3006 3067 2173
Size: 153 634 1 45 61 5578
Fragments arranged by size:
5578 634 153 61 45 1
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
EcoRV GAT'ATC
Cuts at: 2770 2770
Size: 6472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2372 2372
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 5619 1678
Size: 3941 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3638 3670 5113 5145 5187
Size: 32 135 32 2066 32 1443 32 42
Cuts at: 5187 5219 1373
Size: 32 2626
Fragments arranged by size:
2626 2066 1443 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 3816 4062 669
Size: 988 281 580 1298 246 3079
Fragments arranged by size:
3079 1298 988 580 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 2977 3797 2977
Size: 820 5652
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3384 5586 5770 1305
Size: 2079 2202 184 2007
Fragments arranged by size:
2202 2079 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2988 4430 4789 4948 518
Size: 648 1822 1442 359 159 2042
Fragments arranged by size:
2042 1822 1442 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2740 3532 3888 4565 5713 5958 1606
Size: 474 660 792 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 792 677 660 474 356 245
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 3270 513
Size: 552 2205 3715
Fragments arranged by size:
3715 2205 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3830 5375 1132
Size: 97 2601 1545 2229
Fragments arranged by size:
2601 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2549 1348
Size: 1201 5271
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6472
PfoI T'CCnGG_A
Cuts at: 2567 3443 2567
Size: 876 5596
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 2172 2960 3005 3067 2172
Size: 788 45 62 5577
Fragments arranged by size:
5577 788 62 45
PstI C_TGCA'G
Cuts at: 830 3824 830
Size: 2994 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 3297 3820 4955 5636 5827 826
Size: 219 2252 523 1135 681 191 1471
Fragments arranged by size:
2252 1471 1135 681 523 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 2201 1700
Size: 501 5971
SmaI CCC'GGG
Cuts at: 1872 2027 3799 1872
Size: 155 1772 4545
Fragments arranged by size:
4545 1772 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4576 5477 5739 820
Size: 197 926 243 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 2750 1517
Size: 849 384 5239
Fragments arranged by size:
5239 849 384
StyI C'CwwG_G
Cuts at: 513 1065 2745 3270 3911 513
Size: 552 1680 525 641 3074
Fragments arranged by size:
3074 1680 641 552 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2520 3210 3557 4499 4516 4669 4854
Size: 1255 940 690 347 942 17 153 185
Cuts at: 4854 5299 325
Size: 445 1498
Fragments arranged by size:
1498 1255 942 940 690 445 347 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4718 6430 96
Size: 274 80 33 51 1443 2741 1712 138
Fragments arranged by size:
2741 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 6432 6464 2085
Size: 32 4315 32 2093
Fragments arranged by size:
4315 2093 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3197 1316
Size: 1881 4591
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2392 3277 2392
Size: 885 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 2025 3797 1870
Size: 155 1772 4545
Fragments arranged by size:
4545 1772 155
XmnI GAAnn'nnTTC
Cuts at: 2828 3605 4601 2828
Size: 777 996 4699
Fragments arranged by size:
4699 996 777
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI