Restriction map for
pFN21AB9901
(Circular) (Six-base) MAPSORT of: pFN21AB9901.seq Check: 1501 from: 1 to: 5473
pFN21AB9901 5473 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2268 3283 278
Size: 53 83 186 936 732 1015 2468
Fragments arranged by size:
2468 1015 936 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2444 3536 4920 5188 1632
Size: 482 330 1092 1384 268 1917
Fragments arranged by size:
1917 1384 1092 482 330 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 2751 1166
Size: 1585 3888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AlwNI CAG_nnn'CTG
Cuts at: 2033 2089 2311 2413 2488 2497 3216 4788 2033
Size: 56 222 102 75 9 719 1572 2718
Fragments arranged by size:
2718 1572 719 222 102 75 56 9
ApaI G_GGCC'C
Cuts at: 2609 2609
Size: 5473
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2798 1870
Size: 73 855 4545
Fragments arranged by size:
4545 855 73
BamHI G'GATC_C
Cuts at: 2803 3170 2803
Size: 367 5106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2577 2609 2792 1391
Size: 1186 32 183 4072
Fragments arranged by size:
4072 1186 183 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 928
Size: 971 396 4106
Fragments arranged by size:
4106 971 396
BbvCI CC'TCA_GC
Cuts at: 2186 2549 2186
Size: 363 5110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3664 3698 1903
Size: 34 1727 34 3678
Fragments arranged by size:
3678 1727 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4084 136
Size: 107 122 71 631 605 2412 1525
Fragments arranged by size:
2412 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2609 3534 4690 1790
Size: 819 925 1156 2573
Fragments arranged by size:
2573 1156 925 819
BmgBI CAC'GTC
Cuts at: 1318 2199 1318
Size: 881 4592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3204 4162 442
Size: 416 53 83 399 24 1787 958 1753
Fragments arranged by size:
1787 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2269 2493 4133 1239
Size: 880 150 224 1640 2579
Fragments arranged by size:
2579 1640 880 224 150
Bpu10I CC'TnA_GC
Cuts at: 2186 2549 2571 2186
Size: 363 22 5088
Fragments arranged by size:
5088 363 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 4463 4761 3598
Size: 865 298 4310
Fragments arranged by size:
4310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2320 4136 882
Size: 617 821 1816 2219
Fragments arranged by size:
2219 1816 821 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2265 3280 3662 4297
Size: 53 83 186 936 732 1015 382 635
Cuts at: 4297 275
Size: 1451
Fragments arranged by size:
1451 1015 936 732 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2491 2521 2559
Size: 30 165 30 897 30 161 30 38
Cuts at: 2559 2589 1178
Size: 30 4062
Fragments arranged by size:
4062 897 165 161 38 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5473
BseYI C'CCAG_C
Cuts at: 2026 2204 4676 5172 2026
Size: 178 2472 496 2327
Fragments arranged by size:
2472 2327 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2509 1163
Size: 573 773 4127
Fragments arranged by size:
4127 773 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2577 3534 3619 4690 2577
Size: 957 85 1071 3360
Fragments arranged by size:
3360 1071 957 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
BsmBI CGTCTCn'nnnn_
Cuts at: 2205 2205
Size: 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2577 2609 2792 3534 3619 4690 1391
Size: 399 787 32 183 742 85 1071 2174
Fragments arranged by size:
2174 1071 787 742 399 183 85 32
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4117 4304 1700
Size: 110 2307 187 2869
Fragments arranged by size:
2869 2307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4178
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468
Size: 831 367 385 17 1452 86 12 346
Cuts at: 5468 1972
Size: 1977
Fragments arranged by size:
1977 1452 831 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3982
Fragments arranged by size:
3982 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2154 487
Size: 683 56 928 3806
Fragments arranged by size:
3806 928 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2425 2989 3783 3803 2334
Size: 91 564 794 20 4004
Fragments arranged by size:
4004 794 564 91 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 4480 809
Size: 507 948 2216 1802
Fragments arranged by size:
2216 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2920 3809
Size: 54 1425 50 275 18 468 622 889
Cuts at: 3809 5438 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 622 468 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3403 1353
Size: 749 1301 3423
Fragments arranged by size:
3423 1301 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 4434 4580 387
Size: 1064 138 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2575 2575
Size: 5473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2119 2269 2444 2493 3536 4133
Size: 393 482 5 150 175 49 1043 597
Cuts at: 4133 4920 5188 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1043 787 597 482 393 268 175
150 49 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 5473
EcoO109I rG'GnC_Cy
Cuts at: 2308 2464 2494 2605 2606 2688 2733 2308
Size: 156 30 111 1 82 45 5048
Fragments arranged by size:
5048 156 111 82 45 30 1
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 2388 4620 1678
Size: 710 2232 2531
Fragments arranged by size:
2531 2232 710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2407 2439 2491
Size: 32 135 32 728 32 75 32 52
Cuts at: 2491 2523 4114 4146 4188 4220 1373
Size: 32 1591 32 42 32 2626
Fragments arranged by size:
2626 1591 728 135 75 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3651
Fragments arranged by size:
3651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2751 3431 3790 3949 518
Size: 648 1060 525 680 359 159 2042
Fragments arranged by size:
2042 1060 680 648 525 359 159
MspA1I CmG'CkG
Cuts at: 1606 2889 3566 4714 4959 1606
Size: 1283 677 1148 245 2120
Fragments arranged by size:
2120 1283 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3982
Fragments arranged by size:
3982 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2225 2831 4376 1132
Size: 97 996 606 1545 2229
Fragments arranged by size:
2229 1545 996 606 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2033 2311 1108
Size: 925 278 4270
Fragments arranged by size:
4270 925 278
PfoI T'CCnGG_A
Cuts at: 2364 2364
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2464 2688 2308
Size: 156 224 5093
Fragments arranged by size:
5093 224 156
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PspOMI G'GGCC_C
Cuts at: 2605 2605
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2493 2658 2825 830
Size: 1663 165 167 3478
Fragments arranged by size:
3478 1663 167 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
SacI G_AGCT'C
Cuts at: 2577 2577
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SanDI GG'GwC_CC
Cuts at: 2688 2688
Size: 5473
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SexAI A'CCwGG_T
Cuts at: 2292 2310 2292
Size: 18 5455
SfcI C'TryA_G
Cuts at: 826 1045 2388 2489 2654 2821 3956 4637 4828
Size: 219 1343 101 165 167 1135 681 191
Cuts at: 4828 826
Size: 1471
Fragments arranged by size:
1471 1343 1135 681 219 191 167 165
101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 4478 4740 820
Size: 197 926 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5473
StyI C'CwwG_G
Cuts at: 513 1065 2004 2912 513
Size: 552 939 908 3074
Fragments arranged by size:
3074 939 908 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2516 3500 3517 3670 3855 4300
Size: 1255 650 286 984 17 153 185 445
Cuts at: 4300 325
Size: 1498
Fragments arranged by size:
1498 1255 984 650 445 286 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 3719 5431
Size: 274 80 33 51 1443 609 1133 1712
Cuts at: 5431 96
Size: 138
Fragments arranged by size:
1712 1443 1133 609 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 2559 2591 5433 5465 2456
Size: 32 71 32 2842 32 2464
Fragments arranged by size:
2842 2464 71 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 1316
Size: 996 4477
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2266 3281 276
Size: 53 83 186 936 732 1015 2468
Fragments arranged by size:
2468 1015 936 732 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SapI
SfiI SfoI SrfI SwaI XcmI