Restriction map for
pFN21AB9900
(Circular) (Six-base) MAPSORT of: pFN21AB9900.seq Check: 7643 from: 1 to: 5827
pFN21AB9900 5827 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 2955 3090 3148 2955
Size: 135 58 5634
Fragments arranged by size:
5634 135 58
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3019 3890 5274 5542 1632
Size: 1387 871 1384 268 1917
Fragments arranged by size:
1917 1387 1384 871 268
AfeI AGC'GCT
Cuts at: 2435 2435
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2703 4726 5703 1203
Size: 1500 2023 977 1327
Fragments arranged by size:
2023 1500 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 2267 1166
Size: 1101 4726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2038 2077 2469 2680 2968 3570 5142 2038
Size: 39 392 211 288 602 1572 2723
Fragments arranged by size:
2723 1572 602 392 288 211 39
ApaI G_GGCC'C
Cuts at: 2021 2870 2021
Size: 849 4978
ApaLI G'TGCA_C
Cuts at: 2366 3884 5040 2366
Size: 1518 1156 3153
Fragments arranged by size:
3153 1518 1156
ApoI r'AATT_y
Cuts at: 1493 3136 3362 3514 5623 1493
Size: 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 3152 1870
Size: 73 172 1037 4545
Fragments arranged by size:
4545 1037 172 73
AvrII C'CTAG_G
Cuts at: 2138 2138
Size: 5827
BamHI G'GATC_C
Cuts at: 2008 2132 3157 3524 2008
Size: 124 1025 367 4311
Fragments arranged by size:
4311 1025 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2296 2475 2955 3090
Size: 325 217 627 256 253 179 480 135
Cuts at: 3090 3148 4603 618
Size: 58 1455 1842
Fragments arranged by size:
1842 1455 627 480 325 256 253 217
179 135 58
BanII G_rGCy'C
Cuts at: 1391 2021 2179 2870 2939 3146 1391
Size: 630 158 691 69 207 4072
Fragments arranged by size:
4072 691 630 207 158 69
BbeI G_GCGC'C
Cuts at: 2047 2047
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2489 3719 4929 2489
Size: 1230 1210 3387
Fragments arranged by size:
3387 1230 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 2428 2840
Size: 107 122 71 631 605 363 393 412
Cuts at: 2840 4438 136
Size: 1598 1525
Fragments arranged by size:
1598 1525 631 605 412 393 363 122
107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2301 2370 2856 2870 3046 3888 5044
Size: 231 280 69 486 14 176 842 1156
Cuts at: 5044 1790
Size: 2573
Fragments arranged by size:
2573 1156 842 486 280 231 176 69
14
BmgBI CAC'GTC
Cuts at: 1318 2250 2704 1318
Size: 932 454 4441
Fragments arranged by size:
4441 932 454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 2388 3558
Size: 416 53 83 399 24 966 5 1170
Cuts at: 3558 4516 442
Size: 958 1753
Fragments arranged by size:
1753 1170 966 958 416 399 83 53
24 5
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2579 2858 4487 1239
Size: 1078 262 279 1629 2579
Fragments arranged by size:
2579 1629 1078 279 262
Bpu10I CC'TnA_GC
Cuts at: 2993 2993
Size: 5827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 3952 4817 5115 2678
Size: 1274 865 298 3390
Fragments arranged by size:
3390 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2920 3618 493
Size: 711 1716 698 2702
Fragments arranged by size:
2702 1716 711 698
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2907 3634 4016
Size: 53 83 186 936 511 863 727 382
Cuts at: 4016 4651 275
Size: 635 1451
Fragments arranged by size:
1451 936 863 727 635 511 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 2552 2582 2699
Size: 30 165 30 758 30 361 30 117
Cuts at: 2699 2729 2741 2771 1178
Size: 30 12 30 4234
Fragments arranged by size:
4234 758 361 165 117 30 30 30
30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 2462 2863 2870 3039 5030 5526 2462
Size: 401 7 169 1991 496 2763
Fragments arranged by size:
2763 1991 496 401 169 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2310 3277 4038 5066 1540
Size: 275 186 309 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 309 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 3888 3973 5044 2370
Size: 1518 85 1071 3153
Fragments arranged by size:
3153 1518 1071 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2744 3008 2112
Size: 632 264 4931
Fragments arranged by size:
4931 632 264
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2179 2301 2370 2856 2870 2939
Size: 399 231 158 122 69 486 14 69
Cuts at: 2939 3046 3146 3888 3973 5044 1391
Size: 107 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 399 231 158 122
107 100 85 69 69 14
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2967 3182 844
Size: 1268 855 215 3489
Fragments arranged by size:
3489 1268 855 215
BsrBI CCG'CTC
Cuts at: 1394 1823 2593 3714 1394
Size: 429 770 1121 3507
Fragments arranged by size:
3507 1121 770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2472 4471 4658 1700
Size: 110 662 1999 187 2869
Fragments arranged by size:
2869 1999 662 187 110
BsrGI T'GTAC_A
Cuts at: 96 2385 96
Size: 2289 3538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3063 794
Size: 2269 3558
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2947 2947
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2008 2132 2439 3157 3524 3909 3926 5378
Size: 36 124 307 718 367 385 17 1452
Cuts at: 5378 5464 5476 5822 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 307 124
86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2564 2856 513
Size: 552 939 264 296 292 3484
Fragments arranged by size:
3484 939 552 296 292 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 2322 3343 4137 4157 2163
Size: 159 1021 794 20 3833
Fragments arranged by size:
3833 1021 794 159 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2055 2307
Size: 54 1425 50 275 18 192 33 252
Cuts at: 2307 2572 2604 2655 3274 4163 5792 8
Size: 265 32 51 619 889 1629 43
Fragments arranged by size:
1629 1425 889 619 275 265 252 192
54 51 50 43 33 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2307 3274 1537
Size: 275 495 967 4090
Fragments arranged by size:
4090 967 495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3757 1353
Size: 2404 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2579 2858 3019 3890 4487 5274
Size: 393 685 262 279 161 871 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 871 787 685 597 393 279 268
262 161
EcoNI CCTnn'n_nnAGG
Cuts at: 2142 2732 2142
Size: 590 5237
EcoO109I rG'GnC_Cy
Cuts at: 2187 2867 2944 2187
Size: 680 77 5070
Fragments arranged by size:
5070 680 77
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
EcoRV GAT'ATC
Cuts at: 2380 2380
Size: 5827
FspI TGC'GCA
Cuts at: 3104 4333 3104
Size: 1229 4598
HaeII r_GCGC'y
Cuts at: 1678 2047 2437 4974 1678
Size: 369 390 2537 2531
Fragments arranged by size:
2537 2531 390 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2741 2773 4468
Size: 32 135 32 980 32 157 32 1695
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 980 157 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2043 2043
Size: 5827
KpnI G_GTAC'C
Cuts at: 2959 3094 3152 2959
Size: 135 58 5634
Fragments arranged by size:
5634 135 58
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 2661 4941 5125 1305
Size: 1251 105 2280 184 2007
Fragments arranged by size:
2280 2007 1251 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2918 2922 3785 4144 4303 518
Size: 648 1101 651 4 863 359 159 2042
Fragments arranged by size:
2042 1101 863 651 648 359 159 4
MspA1I CmG'CkG
Cuts at: 1606 2049 2074 2193 2283 3039 3243 3920 5068
Size: 443 25 119 90 756 204 677 1148
Cuts at: 5068 5313 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 756 677 443 245 204 119
90 25
NaeI GCC'GGC
Cuts at: 2474 2474
Size: 5827
NarI GG'CG_CC
Cuts at: 2044 2044
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4336
Fragments arranged by size:
4336 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2472 2472
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 5161
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PmlI CAC'GTG
Cuts at: 2920 2920
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2187 2944 2187
Size: 757 5070
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2017 2866 2017
Size: 849 4978
PstI C_TGCA'G
Cuts at: 830 2685 2964 3078 3179 830
Size: 1855 279 114 101 3478
Fragments arranged by size:
3478 1855 279 114 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2074 3039 2074
Size: 965 4862
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SanDI GG'GwC_CC
Cuts at: 2187 2187
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 2685 2964 3179 2685
Size: 279 215 5333
Fragments arranged by size:
5333 279 215
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2681 2960 3074 3175 4310 4991 5182
Size: 219 1636 279 114 101 1135 681 191
Cuts at: 5182 826
Size: 1471
Fragments arranged by size:
1636 1471 1135 681 279 219 191 114
101
SfoI GGC'GCC
Cuts at: 2045 2045
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2693 3931 4832 5094 820
Size: 197 926 750 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 750 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2843 3062 1517
Size: 1326 219 4282
Fragments arranged by size:
4282 1326 219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2138 2733 2940 3266 513
Size: 552 939 134 595 207 326 3074
Fragments arranged by size:
3074 939 595 552 326 207 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2601 2627 3854 3871 4024 4209 4654
Size: 1255 1021 26 1227 17 153 185 445
Cuts at: 4654 325
Size: 1498
Fragments arranged by size:
1498 1255 1227 1021 445 185 153 26
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 4073 5785
Size: 274 80 33 51 1443 408 1688 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1712 1688 1443 408 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 5787 5819 2161
Size: 32 3594 32 2169
Fragments arranged by size:
3594 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2357 2755 1316
Size: 1041 398 4388
Fragments arranged by size:
4388 1041 398
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2037 2614 2011
Size: 26 577 5224
Fragments arranged by size:
5224 577 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI