Restriction map for
pFN21AB9898
(Circular) (Six-base) MAPSORT of: pFN21AB9898.seq Check: 1567 from: 1 to: 10711
pFN21AB9898 10711 bp
With 182 enzymes: SgfI *
February 10, 2017 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5050 5050
Size: 10711
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8521 278
Size: 53 83 186 936 6985 2468
Fragments arranged by size:
6985 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7018 8054 1937
Size: 5081 1036 4594
Fragments arranged by size:
5081 4594 1036
Acc65I G'GTAC_C
Cuts at: 8032 8032
Size: 10711
AclI AA'CG_TT
Cuts at: 1563 8838 9211 1563
Size: 7275 373 3063
Fragments arranged by size:
7275 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2525 3246 3647 3758 4110 4668 5120
Size: 677 216 721 401 111 352 558 452
Cuts at: 5120 5250 6110 6744 7866 8774 10158 10426 1632
Size: 130 860 634 1122 908 1384 268 1917
Fragments arranged by size:
1917 1384 1122 908 860 721 677 634
558 452 401 352 268 216 130 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10514
AflIII A'CryG_T
Cuts at: 1203 3406 9610 10587 1203
Size: 2203 6204 977 1327
Fragments arranged by size:
6204 2203 1327 977
AgeI A'CCGG_T
Cuts at: 9542 9542
Size: 10711
AhdI GACnn_n'nnGTC
Cuts at: 7003 9440 7003
Size: 2437 8274
AleI CACnn'nnGTG
Cuts at: 1166 5664 6073 1166
Size: 4498 409 5804
Fragments arranged by size:
5804 4498 409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3098 6091 1184
Size: 1914 2993 5804
Fragments arranged by size:
5804 2993 1914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6782 6814 6782
Size: 32 10679
AlwNI CAG_nnn'CTG
Cuts at: 2548 2645 4030 4133 5098 5830 6343 6671 8454
Size: 97 1385 103 965 732 513 328 1783
Cuts at: 8454 10026 2548
Size: 1572 3233
Fragments arranged by size:
3233 1783 1572 1385 965 732 513 328
103 97
ApaLI G'TGCA_C
Cuts at: 3983 8768 9924 3983
Size: 4785 1156 4770
Fragments arranged by size:
4785 4770 1156
ApoI r'AATT_y
Cuts at: 1493 3056 3826 4450 5356 6218 6832 8020 8246
Size: 1563 770 624 906 862 614 1188 226
Cuts at: 8246 8398 10507 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1563 1188 906 862 770 624
614 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9265 160
Size: 872 8233 1606
Fragments arranged by size:
8233 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10711
AvaI C'yCGr_G
Cuts at: 1870 1943 7980 8036 1870
Size: 73 6037 56 4545
Fragments arranged by size:
6037 4545 73 56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3887 3920 5358 5391 3887
Size: 33 1438 33 9207
Fragments arranged by size:
9207 1438 33 33
BamHI G'GATC_C
Cuts at: 5833 6695 7570 8041 8408 5833
Size: 862 875 471 367 8136
Fragments arranged by size:
8136 875 862 471 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 5849 6004 6329 8032
Size: 325 217 627 1586 2476 155 325 1703
Cuts at: 8032 9487 618
Size: 1455 1842
Fragments arranged by size:
2476 1842 1703 1586 1455 627 325 325
217 155
BanII G_rGCy'C
Cuts at: 1391 2137 2322 3849 7890 8030 1391
Size: 746 185 1527 4041 140 4072
Fragments arranged by size:
4072 4041 1527 746 185 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6213 928
Size: 971 4314 5426
Fragments arranged by size:
5426 4314 971
BbvCI CC'TCA_GC
Cuts at: 3252 5759 3252
Size: 2507 8204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8902 8936 1903
Size: 34 6965 34 3678
Fragments arranged by size:
6965 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5359 6996 8603 9813 5359
Size: 1637 1607 1210 6257
Fragments arranged by size:
6257 1637 1607 1210
BclI T'GATC_A
Cuts at: 1548 4306 1548
Size: 2758 7953
BfrBI ATG'CAT
Cuts at: 6035 6035
Size: 10711
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3844 7766 9322
Size: 107 122 71 631 605 2172 3922 1556
Cuts at: 9322 136
Size: 1525
Fragments arranged by size:
3922 2172 1556 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3655 4633 10706 3655
Size: 978 6073 3660
Fragments arranged by size:
6073 3660 978
BlpI GC'TnA_GC
Cuts at: 4749 7866 8128 4749
Size: 3117 262 7332
Fragments arranged by size:
7332 3117 262
Bme1580I G_kGCm'C
Cuts at: 1790 3666 3987 8772 9928 1790
Size: 1876 321 4785 1156 2573
Fragments arranged by size:
4785 2573 1876 1156 321
BmgBI CAC'GTC
Cuts at: 1318 6280 1318
Size: 4962 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4088 6952 8442
Size: 416 53 83 399 24 2671 2864 1490
Cuts at: 8442 9400 442
Size: 958 1753
Fragments arranged by size:
2864 2671 1753 1490 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3044 1056
Size: 1988 8723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6475 6475
Size: 10711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 3905 3941 4259 4442 5990 6470 9371
Size: 851 1815 36 318 183 1548 480 2901
Cuts at: 9371 1239
Size: 2579
Fragments arranged by size:
2901 2579 1815 1548 851 480 318 183
36
Bpu10I CC'TnA_GC
Cuts at: 3252 5759 6306 3252
Size: 2507 547 7657
Fragments arranged by size:
7657 2507 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 6795 8836 9701 9999 3524
Size: 3271 2041 865 298 4236
Fragments arranged by size:
4236 3271 2041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5075 6625 9374 882
Size: 617 3576 1550 2749 2219
Fragments arranged by size:
3576 2749 2219 1550 617
BsaAI yAC'GTr
Cuts at: 493 1204 6497 8502 493
Size: 711 5293 2005 2702
Fragments arranged by size:
5293 2702 2005 711
BsaBI GATnn'nnATC
Cuts at: 5690 10705 5690
Size: 5015 5696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8518 8900 9535 275
Size: 53 83 186 936 6985 382 635 1451
Fragments arranged by size:
6985 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 9144 9542 9816 9963 10374 9144
Size: 398 274 147 411 9481
Fragments arranged by size:
9481 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3878 3908 4655 4685 6914
Size: 30 165 30 2475 30 747 30 2229
Cuts at: 6914 6944 7578 7608 7948 7978 1178
Size: 30 634 30 340 30 3911
Fragments arranged by size:
3911 2475 2229 747 634 340 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3222 3225 6326 1186
Size: 2036 3 3101 5571
Fragments arranged by size:
5571 3101 2036 3
BseYI C'CCAG_C
Cuts at: 5239 6579 6588 6618 6636 9914 10410 5239
Size: 1340 9 30 18 3278 496 5540
Fragments arranged by size:
5540 3278 1340 496 30 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 4043 5081 7326 7542 1163
Size: 573 1014 1293 1038 2245 216 4332
Fragments arranged by size:
4332 2245 1293 1038 1014 573 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8161 8922 9950 1540
Size: 275 186 6160 761 1028 2301
Fragments arranged by size:
6160 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2137 3891 3987 6070 6142 8772 8857 9928 2137
Size: 1754 96 2083 72 2630 85 1071 2920
Fragments arranged by size:
2920 2630 2083 1754 1071 96 85 72
BsmI GAATG_Cn'
Cuts at: 8222 8315 8222
Size: 93 10618
BsmBI CGTCTCn'nnnn_
Cuts at: 5747 6709 5747
Size: 962 9749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2322 3666 3849 3891 3987 6070
Size: 399 347 185 1344 183 42 96 2083
Cuts at: 6070 6142 7890 8030 8772 8857 9928 1391
Size: 72 1748 140 742 85 1071 2174
Fragments arranged by size:
2174 2083 1748 1344 1071 742 399 347
185 183 140 96 85 72 42
BspEI T'CCGG_A
Cuts at: 10374 10374
Size: 10711
BspHI T'CATG_A
Cuts at: 7183 8600 10330 7183
Size: 1417 1730 7564
Fragments arranged by size:
7564 1730 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5050 5453 6809 7905 8066 844
Size: 4206 403 1356 1096 161 3489
Fragments arranged by size:
4206 3489 1356 1096 403 161
BsrBI CCG'CTC
Cuts at: 1394 1823 8598 1394
Size: 429 6775 3507
Fragments arranged by size:
6775 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 2937 6910 9206 9380 65
Size: 1183 1473 216 3973 2296 174 1396
Fragments arranged by size:
3973 2296 1473 1396 1183 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5787 9355 9542 1700
Size: 110 3977 3568 187 2869
Fragments arranged by size:
3977 3568 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 7147 96
Size: 7051 3660
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10711
BssSI C'ACGA_G
Cuts at: 3651 8771 9783 3651
Size: 5120 1012 4579
Fragments arranged by size:
5120 4579 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4597 6032 6166 794
Size: 3803 1435 134 5339
Fragments arranged by size:
5339 3803 1435 134
BstBI TT'CG_AA
Cuts at: 9515 9515
Size: 10711
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10711
BstXI CCAn_nnnn'nTGG
Cuts at: 6001 6001
Size: 10711
BstYI r'GATC_y
Cuts at: 1972 3655 4633 5833 5916 6695 6973 7570 7692
Size: 1683 978 1200 83 779 278 597 122
Cuts at: 7692 8041 8408 8793 8810 10262 10348 10360 10706
Size: 349 367 385 17 1452 86 12 346
Cuts at: 10706 1972
Size: 1977
Fragments arranged by size:
1977 1683 1452 1200 978 779 597 385
367 349 346 278 122 86 83 17
12
Bsu36I CC'TnA_GG
Cuts at: 2815 3369 6234 2815
Size: 554 2865 7292
Fragments arranged by size:
7292 2865 554
BtgI C'CryG_G
Cuts at: 513 1065 2004 2655 5085 7593 7837 513
Size: 552 939 651 2430 2508 244 3387
Fragments arranged by size:
3387 2508 2430 939 651 552 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2790 487
Size: 683 56 1564 8408
Fragments arranged by size:
8408 1564 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 2524 2622 2880 3574 3758 5211 5623 5682
Size: 404 98 258 694 184 1453 412 59
Cuts at: 5682 6117 8227 9021 9041 2120
Size: 435 2110 794 20 3790
Fragments arranged by size:
3790 2110 1453 794 694 435 412 404
258 184 98 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10711
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4932 4967 529
Size: 35 4368 35 6273
Fragments arranged by size:
6273 4368 35 35
DraI TTT'AAA
Cuts at: 3117 7662 8015 3117
Size: 4545 353 5813
Fragments arranged by size:
5813 4545 353
DraIII CAC_nnn'GTG
Cuts at: 2163 2672 3982 4034 2163
Size: 509 1310 52 8840
Fragments arranged by size:
8840 1310 509 52
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9718 809
Size: 507 8402 1802
Fragments arranged by size:
8402 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4333 5656 7195
Size: 54 1425 50 275 18 2503 1323 1539
Cuts at: 7195 7590 8158 9047 10676 8
Size: 395 568 889 1629 43
Fragments arranged by size:
2503 1629 1539 1425 1323 889 568 395
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8158 1537
Size: 275 6346 4090
Fragments arranged by size:
6346 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2656 3243 5616 5799 5955 6754 7338 7788
Size: 1303 587 2373 183 156 799 584 450
Cuts at: 7788 8641 1353
Size: 853 3423
Fragments arranged by size:
3423 2373 1303 853 799 587 584 450
183 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9298 9672 9818 387
Size: 1064 138 7709 374 146 1280
Fragments arranged by size:
7709 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2135 2135
Size: 10711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2309 2525 3246 3647 3758 3905
Size: 393 458 219 216 721 401 111 147
Cuts at: 3905 3941 4110 4259 4442 4668 5120 5250 5990
Size: 36 169 149 183 226 452 130 740
Cuts at: 5990 6110 6470 6744 7866 8774 9371 10158 10426
Size: 120 360 274 1122 908 597 787 268
Cuts at: 10426 1239
Size: 1524
Fragments arranged by size:
1524 1122 908 787 740 721 597 458
452 401 393 360 274 268 226 219
216 183 169 149 147 130 120 111
36
EcoNI CCTnn'n_nnAGG
Cuts at: 3264 3264
Size: 10711
EcoO109I rG'GnC_Cy
Cuts at: 3709 5438 5465 7991 3709
Size: 1729 27 2526 6429
Fragments arranged by size:
6429 2526 1729 27
EcoRI G'AATT_C
Cuts at: 3056 6832 8020 8398 3056
Size: 3776 1188 378 5369
Fragments arranged by size:
5369 3776 1188 378
EcoRV GAT'ATC
Cuts at: 6766 6766
Size: 10711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3193 3876 4655 3193
Size: 683 779 9249
Fragments arranged by size:
9249 779 683
FspI TGC'GCA
Cuts at: 4740 9217 4740
Size: 4477 6234
HaeII r_GCGC'y
Cuts at: 1678 9858 1678
Size: 8180 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2122 2154 3379
Size: 32 135 32 502 32 16 32 1225
Cuts at: 3379 3411 3878 3910 4198 4230 4270 4302 4537
Size: 32 467 32 288 32 40 32 235
Cuts at: 4537 4569 4655 4687 5381 5413 6914 6946 6989
Size: 32 86 32 694 32 1501 32 43
Cuts at: 6989 7021 7529 7561 9352 9384 9426 9458 1373
Size: 32 508 32 1791 32 42 32 2626
Fragments arranged by size:
2626 1791 1501 1225 694 508 502 467
288 235 135 86 43 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2417 4730 6676 8055 8301 669
Size: 988 281 479 2313 1946 1379 246 3079
Fragments arranged by size:
3079 2313 1946 1379 988 479 281 246
HindIII A'AGCT_T
Cuts at: 748 3858 4680 6437 748
Size: 3110 822 1757 5022
Fragments arranged by size:
5022 3110 1757 822
HpaI GTT'AAC
Cuts at: 8301 8301
Size: 10711
KpnI G_GTAC'C
Cuts at: 8036 8036
Size: 10711
MfeI C'AATT_G
Cuts at: 3212 8310 3212
Size: 5098 5613
MluI A'CGCG_T
Cuts at: 10587 10587
Size: 10711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3855 7625 9825 10009 1305
Size: 2550 3770 2200 184 2007
Fragments arranged by size:
3770 2550 2200 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4335 5658 7197 10
Size: 54 1425 343 2503 1323 1539 3524
Fragments arranged by size:
3524 2503 1539 1425 1323 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 2726 2751 3409 4773 4779 5664
Size: 648 1501 59 25 658 1364 6 885
Cuts at: 5664 5696 5999 6073 6187 6898 7934 8669 9028
Size: 32 303 74 114 711 1036 735 359
Cuts at: 9028 9187 518
Size: 159 2042
Fragments arranged by size:
2042 1501 1364 1036 885 735 711 658
648 359 303 159 114 74 59 32
25 6
MspA1I CmG'CkG
Cuts at: 1606 2870 3883 5294 5716 6431 8127 8804 9952
Size: 1264 1013 1411 422 715 1696 677 1148
Cuts at: 9952 10197 1606
Size: 245 2120
Fragments arranged by size:
2120 1696 1411 1264 1148 1013 715 677
422 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5085 7837 513
Size: 552 939 3081 2752 3387
Fragments arranged by size:
3387 3081 2752 939 552
NdeI CA'TA_TG
Cuts at: 387 7969 387
Size: 7582 3129
NheI G'CTAG_C
Cuts at: 1052 3040 1052
Size: 1988 8723
NotI GC'GGCC_GC
Cuts at: 8158 8158
Size: 10711
NsiI A_TGCA'T
Cuts at: 6037 6037
Size: 10711
NspI r_CATG'y
Cuts at: 1132 1229 3410 3838 4488 4600 4853 4976 5884
Size: 97 2181 428 650 112 253 123 908
Cuts at: 5884 6126 6169 7321 7372 7649 8069 9614 1132
Size: 242 43 1152 51 277 420 1545 2229
Fragments arranged by size:
2229 2181 1545 1152 908 650 428 420
277 253 242 123 112 97 51 43
PasI CC'CwG_GG
Cuts at: 1348 6860 1348
Size: 5512 5199
PciI A'CATG_T
Cuts at: 3406 9610 3406
Size: 6204 4507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3560 7052 7204 7510 7607 7739 1108
Size: 2452 3492 152 306 97 132 4080
Fragments arranged by size:
4080 3492 2452 306 152 132 97
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 10711
PmeI GTTT'AAAC
Cuts at: 8015 8015
Size: 10711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2295 2327 8780 8812 10293 10325 2295
Size: 32 6453 32 1481 32 2681
Fragments arranged by size:
6453 2681 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5438 5465 5438
Size: 27 10684
PshAI GACnn'nnGTC
Cuts at: 1613 3526 1613
Size: 1913 8798
PsiI TTA'TAA
Cuts at: 8281 8281
Size: 10711
PstI C_TGCA'G
Cuts at: 830 2519 2875 3013 3484 5221 5677 6430 6820
Size: 1689 356 138 471 1737 456 753 390
Cuts at: 6820 8063 830
Size: 1243 3478
Fragments arranged by size:
3478 1737 1689 1243 753 471 456 390
356 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10711
PvuII CAG'CTG
Cuts at: 2870 3883 5294 6431 2870
Size: 1013 1411 1137 7150
Fragments arranged by size:
7150 1411 1137 1013
SacI G_AGCT'C
Cuts at: 2137 2137
Size: 10711
SalI G'TCGA_C
Cuts at: 1936 8053 1936
Size: 6117 4594
SbfI CC_TGCA'GG
Cuts at: 8063 8063
Size: 10711
ScaI AGT'ACT
Cuts at: 8959 10671 8959
Size: 1712 8999
SexAI A'CCwGG_T
Cuts at: 3978 3978
Size: 10711
SfcI C'TryA_G
Cuts at: 826 1045 2463 2515 2637 2871 3009 3480 3578
Size: 219 1418 52 122 234 138 471 98
Cuts at: 3578 4691 5217 5673 6426 6596 6816 7300 8059
Size: 1113 526 456 753 170 220 484 759
Cuts at: 8059 9194 9875 10066 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1418 1135 1113 759 753 681 526
484 471 456 234 220 219 191 170
138 122 98 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10711
SgrAI Cr'CCGG_yG
Cuts at: 1700 5787 1700
Size: 4087 6624
SmaI CCC'GGG
Cuts at: 1872 8038 1872
Size: 6166 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3539 6810 8815 9716 9978 820
Size: 197 926 1596 3271 2005 901 262 1553
Fragments arranged by size:
3271 2005 1596 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 8502 493
Size: 8009 2702
SpeI A'CTAG_T
Cuts at: 152 7498 152
Size: 7346 3365
SphI G_CATG'C
Cuts at: 1132 4488 4853 7649 8069 1132
Size: 3356 365 2796 420 3774
Fragments arranged by size:
3774 3356 2796 420 365
SspI AAT'ATT
Cuts at: 5 52 2702 4604 8635 5
Size: 47 2650 1902 4031 2081
Fragments arranged by size:
4031 2650 2081 1902 47
StuI AGG'CCT
Cuts at: 1517 2888 3442 1517
Size: 1371 554 8786
Fragments arranged by size:
8786 1371 554
StyI C'CwwG_G
Cuts at: 513 1065 2004 3851 3934 5085 5921 7046 7441
Size: 552 939 1847 83 1151 836 1125 395
Cuts at: 7441 7837 8150 513
Size: 396 313 3074
Fragments arranged by size:
3074 1847 1151 1125 939 836 552 396
395 313 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2817 3881 5587 7039 7243 8738 8755
Size: 1255 1237 1064 1706 1452 204 1495 17
Cuts at: 8755 8908 9093 9538 325
Size: 153 185 445 1498
Fragments arranged by size:
1706 1498 1495 1452 1255 1237 1064 445
204 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4429 5883 7147
Size: 274 80 33 51 1443 2452 1454 1264
Cuts at: 7147 8957 10669 96
Size: 1810 1712 138
Fragments arranged by size:
2452 1810 1712 1454 1443 1264 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7578 7610 10671 10703 7578
Size: 32 3061 32 7586
Fragments arranged by size:
7586 3061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2046 6667 7930 1316
Size: 730 4621 1263 4097
Fragments arranged by size:
4621 4097 1263 730
XbaI T'CTAG_A
Cuts at: 7217 8047 7217
Size: 830 9881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 4721 7049 7186 7385 2357
Size: 2364 2328 137 199 5683
Fragments arranged by size:
5683 2364 2328 199 137
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10711
XmaI C'CCGG_G
Cuts at: 1870 8036 1870
Size: 6166 4545
XmnI GAAnn'nnTTC
Cuts at: 4356 8840 4356
Size: 4484 6227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8519 276
Size: 53 83 186 936 6985 2468
Fragments arranged by size:
6985 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI